Investigation of Plant Interactions Across Common Mycorrhizal Networks Using Rotated Cores.
Identifieur interne : 000488 ( Main/Exploration ); précédent : 000487; suivant : 000489Investigation of Plant Interactions Across Common Mycorrhizal Networks Using Rotated Cores.
Auteurs : Joanna Weremijewicz [Oman] ; David P. JanosSource :
- Journal of visualized experiments : JoVE [ 1940-087X ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Plant, Racines de plante.
- microbiologie : Plant, Racines de plante.
- physiologie : Mycorhizes.
- Interactions hôte-pathogène, Matières plastiques, Écosystème.
English descriptors
- KwdEn :
- MESH :
- chemical : Plastics.
- growth & development : Plant Roots, Seedlings.
- microbiology : Plant Roots, Seedlings.
- physiology : Mycorrhizae.
- Ecosystem, Host-Pathogen Interactions.
Abstract
Arbuscular mycorrhizal (AM) fungi influence plant mineral nutrient uptake and growth, hence, they have the potential to influence plant interactions. The power of their influence is in extraradical mycelia that spread beyond nutrient depletion zones found near roots to ultimately interconnect individuals within a common mycorrhizal network (CMN). Most experiments, however, have investigated the role of AM fungi in plant interactions by growing plants with versus without mycorrhizal fungi, a method that fails to explicitly address the role of CMNs. Here, we propose a method that manipulates CMNs to investigate their role in plant interactions. Our method uses modified containers with conical bottoms with a nylon mesh and/or hydrophobic material covering slotted openings, 15N fertilizer, and a nutrient-poor interstitial sand. CMNs are left either intact between interacting individuals, severed by rotation of containers, or prevented from forming by a solid barrier. Our findings suggest that rotating containers is sufficient to disrupt CMNs and prevent their effects on plant interactions across CMNs. Our approach is advantageous because it mimics aspects of nature, such as seedlings tapping into already established CMNs and the use of a suite of AM fungi that may provide diverse benefits. Although our experiment is limited to investigating plants at the seedling stage, plant interactions across CMNs can be detected using our approach which therefore can be applied to investigate biological questions about the functioning of CMNs in ecosystems.
DOI: 10.3791/59338
PubMed: 30985758
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Investigation of Plant Interactions Across Common Mycorrhizal Networks Using Rotated Cores.</title><author><name sortKey="Weremijewicz, Joanna" sort="Weremijewicz, Joanna" uniqKey="Weremijewicz J" first="Joanna" last="Weremijewicz">Joanna Weremijewicz</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, North Central College; Department of Biology, University of Miami; jweremi@gmail.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Department of Biology, North Central College; Department of Biology</wicri:regionArea><wicri:noRegion>North Central College; Department of Biology</wicri:noRegion></affiliation></author><author><name sortKey="Janos, David P" sort="Janos, David P" uniqKey="Janos D" first="David P" last="Janos">David P. Janos</name><affiliation><nlm:affiliation>Department of Biology, University of Miami.</nlm:affiliation><wicri:noCountry code="subField">University of Miami</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30985758</idno><idno type="pmid">30985758</idno><idno type="doi">10.3791/59338</idno><idno type="wicri:Area/Main/Corpus">000493</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000493</idno><idno type="wicri:Area/Main/Curation">000493</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000493</idno><idno type="wicri:Area/Main/Exploration">000493</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Investigation of Plant Interactions Across Common Mycorrhizal Networks Using Rotated Cores.</title><author><name sortKey="Weremijewicz, Joanna" sort="Weremijewicz, Joanna" uniqKey="Weremijewicz J" first="Joanna" last="Weremijewicz">Joanna Weremijewicz</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, North Central College; Department of Biology, University of Miami; jweremi@gmail.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Department of Biology, North Central College; Department of Biology</wicri:regionArea><wicri:noRegion>North Central College; Department of Biology</wicri:noRegion></affiliation></author><author><name sortKey="Janos, David P" sort="Janos, David P" uniqKey="Janos D" first="David P" last="Janos">David P. Janos</name><affiliation><nlm:affiliation>Department of Biology, University of Miami.</nlm:affiliation><wicri:noCountry code="subField">University of Miami</wicri:noCountry></affiliation></author></analytic><series><title level="j">Journal of visualized experiments : JoVE</title><idno type="eISSN">1940-087X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ecosystem (MeSH)</term><term>Host-Pathogen Interactions (MeSH)</term><term>Mycorrhizae (physiology)</term><term>Plant Roots (growth & development)</term><term>Plant Roots (microbiology)</term><term>Plastics (MeSH)</term><term>Seedlings (growth & development)</term><term>Seedlings (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Interactions hôte-pathogène (MeSH)</term><term>Matières plastiques (MeSH)</term><term>Mycorhizes (physiologie)</term><term>Plant (croissance et développement)</term><term>Plant (microbiologie)</term><term>Racines de plante (croissance et développement)</term><term>Racines de plante (microbiologie)</term><term>Écosystème (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Plastics</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Plant</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Roots</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Plant</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Roots</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Ecosystem</term><term>Host-Pathogen Interactions</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Interactions hôte-pathogène</term><term>Matières plastiques</term><term>Écosystème</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Arbuscular mycorrhizal (AM) fungi influence plant mineral nutrient uptake and growth, hence, they have the potential to influence plant interactions. The power of their influence is in extraradical mycelia that spread beyond nutrient depletion zones found near roots to ultimately interconnect individuals within a common mycorrhizal network (CMN). Most experiments, however, have investigated the role of AM fungi in plant interactions by growing plants with versus without mycorrhizal fungi, a method that fails to explicitly address the role of CMNs. Here, we propose a method that manipulates CMNs to investigate their role in plant interactions. Our method uses modified containers with conical bottoms with a nylon mesh and/or hydrophobic material covering slotted openings, <sup>15</sup>N fertilizer, and a nutrient-poor interstitial sand. CMNs are left either intact between interacting individuals, severed by rotation of containers, or prevented from forming by a solid barrier. Our findings suggest that rotating containers is sufficient to disrupt CMNs and prevent their effects on plant interactions across CMNs. Our approach is advantageous because it mimics aspects of nature, such as seedlings tapping into already established CMNs and the use of a suite of AM fungi that may provide diverse benefits. Although our experiment is limited to investigating plants at the seedling stage, plant interactions across CMNs can be detected using our approach which therefore can be applied to investigate biological questions about the functioning of CMNs in ecosystems.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">30985758</PMID><DateCompleted><Year>2020</Year><Month>02</Month><Day>05</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>05</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1940-087X</ISSN><JournalIssue CitedMedium="Internet"><Issue>145</Issue><PubDate><Year>2019</Year><Month>03</Month><Day>26</Day></PubDate></JournalIssue><Title>Journal of visualized experiments : JoVE</Title><ISOAbbreviation>J Vis Exp</ISOAbbreviation></Journal><ArticleTitle>Investigation of Plant Interactions Across Common Mycorrhizal Networks Using Rotated Cores.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.3791/59338</ELocationID><Abstract><AbstractText>Arbuscular mycorrhizal (AM) fungi influence plant mineral nutrient uptake and growth, hence, they have the potential to influence plant interactions. The power of their influence is in extraradical mycelia that spread beyond nutrient depletion zones found near roots to ultimately interconnect individuals within a common mycorrhizal network (CMN). Most experiments, however, have investigated the role of AM fungi in plant interactions by growing plants with versus without mycorrhizal fungi, a method that fails to explicitly address the role of CMNs. Here, we propose a method that manipulates CMNs to investigate their role in plant interactions. Our method uses modified containers with conical bottoms with a nylon mesh and/or hydrophobic material covering slotted openings, <sup>15</sup>N fertilizer, and a nutrient-poor interstitial sand. CMNs are left either intact between interacting individuals, severed by rotation of containers, or prevented from forming by a solid barrier. Our findings suggest that rotating containers is sufficient to disrupt CMNs and prevent their effects on plant interactions across CMNs. Our approach is advantageous because it mimics aspects of nature, such as seedlings tapping into already established CMNs and the use of a suite of AM fungi that may provide diverse benefits. Although our experiment is limited to investigating plants at the seedling stage, plant interactions across CMNs can be detected using our approach which therefore can be applied to investigate biological questions about the functioning of CMNs in ecosystems.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Weremijewicz</LastName><ForeName>Joanna</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Biology, North Central College; Department of Biology, University of Miami; jweremi@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Janos</LastName><ForeName>David P</ForeName><Initials>DP</Initials><AffiliationInfo><Affiliation>Department of Biology, University of Miami.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType><PublicationType UI="D059040">Video-Audio Media</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>03</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Vis Exp</MedlineTA><NlmUniqueID>101313252</NlmUniqueID><ISSNLinking>1940-087X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010969">Plastics</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017753" MajorTopicYN="N">Ecosystem</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="Y">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010969" MajorTopicYN="N">Plastics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D036226" MajorTopicYN="N">Seedlings</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>4</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>2</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30985758</ArticleId><ArticleId IdType="doi">10.3791/59338</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Oman</li></country></list><tree><noCountry><name sortKey="Janos, David P" sort="Janos, David P" uniqKey="Janos D" first="David P" last="Janos">David P. Janos</name></noCountry><country name="Oman"><noRegion><name sortKey="Weremijewicz, Joanna" sort="Weremijewicz, Joanna" uniqKey="Weremijewicz J" first="Joanna" last="Weremijewicz">Joanna Weremijewicz</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MycorrhizaeV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000488 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000488 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MycorrhizaeV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30985758
|texte= Investigation of Plant Interactions Across Common Mycorrhizal Networks Using Rotated Cores.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30985758" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MycorrhizaeV1
| This area was generated with Dilib version V0.6.37. |  |