Ectomycorrhizal community structure in a xeric Quercus woodland based on rDNA sequence analysis of sporocarps and pooled roots.
Identifieur interne : 002F40 ( Main/Curation ); précédent : 002F39; suivant : 002F41Ectomycorrhizal community structure in a xeric Quercus woodland based on rDNA sequence analysis of sporocarps and pooled roots.
Auteurs : Matthew E. Smith [États-Unis] ; Greg W. Douhan ; David M. RizzoSource :
- The New phytologist [ 0028-646X ] ; 2007.
Descripteurs français
- KwdFr :
- ADN des plantes (génétique), ADN ribosomique (génétique), Aliment pour animaux (MeSH), Animaux (MeSH), Bovins (MeSH), Californie (MeSH), Climat (MeSH), Mycorhizes (génétique), Mycorhizes (isolement et purification), Polymorphisme de restriction (MeSH), Quercus (génétique), Quercus (microbiologie), Quercus (physiologie), Racines de plante (génétique), Racines de plante (physiologie), Réaction de polymérisation en chaîne (MeSH), Région méditerranéenne (MeSH), Variation génétique (MeSH).
- MESH :
- génétique : ADN des plantes, ADN ribosomique, Mycorhizes, Quercus, Racines de plante.
- isolement et purification : Mycorhizes.
- microbiologie : Quercus.
- physiologie : Quercus, Racines de plante.
- Aliment pour animaux, Animaux, Bovins, Californie, Climat, Polymorphisme de restriction, Réaction de polymérisation en chaîne, Région méditerranéenne, Variation génétique.
English descriptors
- KwdEn :
- Animal Feed (MeSH), Animals (MeSH), California (MeSH), Cattle (MeSH), Climate (MeSH), DNA, Plant (genetics), DNA, Ribosomal (genetics), Genetic Variation (MeSH), Mediterranean Region (MeSH), Mycorrhizae (genetics), Mycorrhizae (isolation & purification), Plant Roots (genetics), Plant Roots (physiology), Polymerase Chain Reaction (MeSH), Polymorphism, Restriction Fragment Length (MeSH), Quercus (genetics), Quercus (microbiology), Quercus (physiology).
- MESH :
- chemical , genetics : DNA, Plant, DNA, Ribosomal.
- geographic : California, Mediterranean Region.
- genetics : Mycorrhizae, Plant Roots, Quercus.
- isolation & purification : Mycorrhizae.
- microbiology : Quercus.
- physiology : Plant Roots, Quercus.
- Animal Feed, Animals, Cattle, Climate, Genetic Variation, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length.
Abstract
Quercus woodlands are key components of California's wild landscapes, yet little is known about ectomycorrhizal (EM) fungi in these ecosystems. We examined the EM community associated with Quercus douglasii using sporocarp surveys and by pooling EM roots and subjecting them to DNA extraction, polymerase chain reaction (PCR), cloning, restriction fragment length polymorphism (RFLP) screening and DNA sequencing. Ectomycorrhizal root symbionts were sampled four times in 2003-04. During this time, the below-ground community structure was relatively stable; we found no evidence of taxa adapted to winter or spring conditions and only one species varied widely in occurrence between years. The EM community from sporocarps and roots was diverse (161 species), rich in Ascomycota (46 species), and dominated by fungi with cryptic sporocarps. This included a large number of resupinate and hypogeous taxa, many of which were detected both above- and below-ground.
DOI: 10.1111/j.1469-8137.2007.02040.x
PubMed: 17504467
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We examined the EM community associated with Quercus douglasii using sporocarp surveys and by pooling EM roots and subjecting them to DNA extraction, polymerase chain reaction (PCR), cloning, restriction fragment length polymorphism (RFLP) screening and DNA sequencing. Ectomycorrhizal root symbionts were sampled four times in 2003-04. During this time, the below-ground community structure was relatively stable; we found no evidence of taxa adapted to winter or spring conditions and only one species varied widely in occurrence between years. The EM community from sporocarps and roots was diverse (161 species), rich in Ascomycota (46 species), and dominated by fungi with cryptic sporocarps. This included a large number of resupinate and hypogeous taxa, many of which were detected both above- and below-ground.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17504467</PMID><DateCompleted><Year>2007</Year><Month>08</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0028-646X</ISSN><JournalIssue CitedMedium="Print"><Volume>174</Volume><Issue>4</Issue><PubDate><Year>2007</Year></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Ectomycorrhizal community structure in a xeric Quercus woodland based on rDNA sequence analysis of sporocarps and pooled roots.</ArticleTitle><Pagination><MedlinePgn>847-63</MedlinePgn></Pagination><Abstract><AbstractText>Quercus woodlands are key components of California's wild landscapes, yet little is known about ectomycorrhizal (EM) fungi in these ecosystems. 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