Laser microdissection reveals that transcripts for five plant and one fungal phosphate transporter genes are contemporaneously present in arbusculated cells.
Identifieur interne : 002E43 ( Main/Curation ); précédent : 002E42; suivant : 002E44Laser microdissection reveals that transcripts for five plant and one fungal phosphate transporter genes are contemporaneously present in arbusculated cells.
Auteurs : Raffaella Balestrini [Italie] ; Jorge G Mez-Ariza ; Luisa Lanfranco ; Paola BonfanteSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2007.
Descripteurs français
- KwdFr :
- ARN des plantes (génétique), ARN des plantes (métabolisme), ARN fongique (génétique), ARN fongique (métabolisme), ARN messager (génétique), ARN messager (métabolisme), Lasers (MeSH), Lycopersicon esculentum (cytologie), Lycopersicon esculentum (microbiologie), Microdissection (MeSH), Mycorhizes (cytologie), Mycorhizes (métabolisme), Protéines de transport du phosphate (génétique), Protéines fongiques (génétique), Protéines végétales (génétique), Racines de plante (cytologie), Régulation de l'expression des gènes fongiques (MeSH), Régulation de l'expression des gènes végétaux (MeSH).
- MESH :
- cytologie : Lycopersicon esculentum, Mycorhizes, Racines de plante.
- génétique : ARN des plantes, ARN fongique, ARN messager, Protéines de transport du phosphate, Protéines fongiques, Protéines végétales.
- microbiologie : Lycopersicon esculentum.
- métabolisme : ARN des plantes, ARN fongique, ARN messager, Mycorhizes.
- Lasers, Microdissection, Régulation de l'expression des gènes fongiques, Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- Fungal Proteins (genetics), Gene Expression Regulation, Fungal (MeSH), Gene Expression Regulation, Plant (MeSH), Lasers (MeSH), Lycopersicon esculentum (cytology), Lycopersicon esculentum (microbiology), Microdissection (MeSH), Mycorrhizae (cytology), Mycorrhizae (metabolism), Phosphate Transport Proteins (genetics), Plant Proteins (genetics), Plant Roots (cytology), RNA, Fungal (genetics), RNA, Fungal (metabolism), RNA, Messenger (genetics), RNA, Messenger (metabolism), RNA, Plant (genetics), RNA, Plant (metabolism).
- MESH :
- chemical , genetics : Fungal Proteins, Phosphate Transport Proteins, Plant Proteins, RNA, Fungal, RNA, Messenger, RNA, Plant.
- cytology : Lycopersicon esculentum, Mycorrhizae, Plant Roots.
- metabolism : Mycorrhizae, RNA, Fungal, RNA, Messenger, RNA, Plant.
- microbiology : Lycopersicon esculentum.
- Gene Expression Regulation, Fungal, Gene Expression Regulation, Plant, Lasers, Microdissection.
Abstract
The establishment of a symbiotic interaction between plant roots and arbuscular mycorrhizal (AM) fungi requires both partners to undergo significant morphological and physiological modifications which eventually lead to reciprocal beneficial effects. Extensive changes in gene expression profiles recently have been described in transcriptomic studies that have analyzed the whole mycorrhizal root. However, because root colonization by AM fungi involves different cell types, a cell-specific gene expression pattern is likely to occur. We have applied the laser microdissection (LMD) technology to investigate expression profiles of both plant and fungal genes in Lycopersicon esculentum roots colonized by Glomus mosseae. A protocol to harvest arbuscule-containing cells from paraffin sections of mycorrhizal roots has been developed using a Leica AS LMD system. RNA of satisfactory quantity and quality has been extracted for molecular analysis. Transcripts for plant phosphate transporters (LePTs), selected as molecular markers for a functional symbiosis, have been detected by reverse-transcriptase polymerase chain reaction assays and associated to distinct cell types, leading to novel insights into the distribution of LePT mRNAs. In fact, the transcripts of the five phosphate transporters (PTs) have been detected contemporaneously in the same arbusculated cell population, unlike from the neighboring noncolonized cells. In addition, fungal H(+)ATPase (GmHA5) and phosphate transporter (GmosPT) mRNAs were found exclusively in arbusculated cells. The discovery that five plant and one fungal PT genes are consistently expressed inside the arbusculated cells provides a new scenario for plant-fungus nutrient exchanges.
DOI: 10.1094/MPMI-20-9-1055
PubMed: 17849708
Links toward previous steps (curation, corpus...)
- to stream Main, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002E43
Links to Exploration step
pubmed:17849708Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Laser microdissection reveals that transcripts for five plant and one fungal phosphate transporter genes are contemporaneously present in arbusculated cells.</title><author><name sortKey="Balestrini, Raffaella" sort="Balestrini, Raffaella" uniqKey="Balestrini R" first="Raffaella" last="Balestrini">Raffaella Balestrini</name><affiliation wicri:level="1"><nlm:affiliation>Istituto Protezione Piante, CNR and Dipartimento di Biologia Vegetale, Università di Torino, Viale Mattioli, 25-10125 Torino, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto Protezione Piante, CNR and Dipartimento di Biologia Vegetale, Università di Torino, Viale Mattioli, 25-10125 Torino</wicri:regionArea></affiliation></author><author><name sortKey="G Mez Ariza, Jorge" sort="G Mez Ariza, Jorge" uniqKey="G Mez Ariza J" first="Jorge" last="G Mez-Ariza">Jorge G Mez-Ariza</name></author><author><name sortKey="Lanfranco, Luisa" sort="Lanfranco, Luisa" uniqKey="Lanfranco L" first="Luisa" last="Lanfranco">Luisa Lanfranco</name></author><author><name sortKey="Bonfante, Paola" sort="Bonfante, Paola" uniqKey="Bonfante P" first="Paola" last="Bonfante">Paola Bonfante</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17849708</idno><idno type="pmid">17849708</idno><idno type="doi">10.1094/MPMI-20-9-1055</idno><idno type="wicri:Area/Main/Corpus">002E43</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002E43</idno><idno type="wicri:Area/Main/Curation">002E43</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002E43</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Laser microdissection reveals that transcripts for five plant and one fungal phosphate transporter genes are contemporaneously present in arbusculated cells.</title><author><name sortKey="Balestrini, Raffaella" sort="Balestrini, Raffaella" uniqKey="Balestrini R" first="Raffaella" last="Balestrini">Raffaella Balestrini</name><affiliation wicri:level="1"><nlm:affiliation>Istituto Protezione Piante, CNR and Dipartimento di Biologia Vegetale, Università di Torino, Viale Mattioli, 25-10125 Torino, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto Protezione Piante, CNR and Dipartimento di Biologia Vegetale, Università di Torino, Viale Mattioli, 25-10125 Torino</wicri:regionArea></affiliation></author><author><name sortKey="G Mez Ariza, Jorge" sort="G Mez Ariza, Jorge" uniqKey="G Mez Ariza J" first="Jorge" last="G Mez-Ariza">Jorge G Mez-Ariza</name></author><author><name sortKey="Lanfranco, Luisa" sort="Lanfranco, Luisa" uniqKey="Lanfranco L" first="Luisa" last="Lanfranco">Luisa Lanfranco</name></author><author><name sortKey="Bonfante, Paola" sort="Bonfante, Paola" uniqKey="Bonfante P" first="Paola" last="Bonfante">Paola Bonfante</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fungal Proteins (genetics)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Lasers (MeSH)</term><term>Lycopersicon esculentum (cytology)</term><term>Lycopersicon esculentum (microbiology)</term><term>Microdissection (MeSH)</term><term>Mycorrhizae (cytology)</term><term>Mycorrhizae (metabolism)</term><term>Phosphate Transport Proteins (genetics)</term><term>Plant Proteins (genetics)</term><term>Plant Roots (cytology)</term><term>RNA, Fungal (genetics)</term><term>RNA, Fungal (metabolism)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>RNA, Plant (genetics)</term><term>RNA, Plant (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (génétique)</term><term>ARN des plantes (métabolisme)</term><term>ARN fongique (génétique)</term><term>ARN fongique (métabolisme)</term><term>ARN messager (génétique)</term><term>ARN messager (métabolisme)</term><term>Lasers (MeSH)</term><term>Lycopersicon esculentum (cytologie)</term><term>Lycopersicon esculentum (microbiologie)</term><term>Microdissection (MeSH)</term><term>Mycorhizes (cytologie)</term><term>Mycorhizes (métabolisme)</term><term>Protéines de transport du phosphate (génétique)</term><term>Protéines fongiques (génétique)</term><term>Protéines végétales (génétique)</term><term>Racines de plante (cytologie)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>Phosphate Transport Proteins</term><term>Plant Proteins</term><term>RNA, Fungal</term><term>RNA, Messenger</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Lycopersicon esculentum</term><term>Mycorhizes</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Lycopersicon esculentum</term><term>Mycorrhizae</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term><term>ARN fongique</term><term>ARN messager</term><term>Protéines de transport du phosphate</term><term>Protéines fongiques</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Mycorrhizae</term><term>RNA, Fungal</term><term>RNA, Messenger</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lycopersicon esculentum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lycopersicon esculentum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN des plantes</term><term>ARN fongique</term><term>ARN messager</term><term>Mycorhizes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Fungal</term><term>Gene Expression Regulation, Plant</term><term>Lasers</term><term>Microdissection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Lasers</term><term>Microdissection</term><term>Régulation de l'expression des gènes fongiques</term><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The establishment of a symbiotic interaction between plant roots and arbuscular mycorrhizal (AM) fungi requires both partners to undergo significant morphological and physiological modifications which eventually lead to reciprocal beneficial effects. Extensive changes in gene expression profiles recently have been described in transcriptomic studies that have analyzed the whole mycorrhizal root. However, because root colonization by AM fungi involves different cell types, a cell-specific gene expression pattern is likely to occur. We have applied the laser microdissection (LMD) technology to investigate expression profiles of both plant and fungal genes in Lycopersicon esculentum roots colonized by Glomus mosseae. A protocol to harvest arbuscule-containing cells from paraffin sections of mycorrhizal roots has been developed using a Leica AS LMD system. RNA of satisfactory quantity and quality has been extracted for molecular analysis. Transcripts for plant phosphate transporters (LePTs), selected as molecular markers for a functional symbiosis, have been detected by reverse-transcriptase polymerase chain reaction assays and associated to distinct cell types, leading to novel insights into the distribution of LePT mRNAs. In fact, the transcripts of the five phosphate transporters (PTs) have been detected contemporaneously in the same arbusculated cell population, unlike from the neighboring noncolonized cells. In addition, fungal H(+)ATPase (GmHA5) and phosphate transporter (GmosPT) mRNAs were found exclusively in arbusculated cells. The discovery that five plant and one fungal PT genes are consistently expressed inside the arbusculated cells provides a new scenario for plant-fungus nutrient exchanges.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17849708</PMID><DateCompleted><Year>2007</Year><Month>10</Month><Day>16</Day></DateCompleted><DateRevised><Year>2007</Year><Month>09</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>20</Volume><Issue>9</Issue><PubDate><Year>2007</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Laser microdissection reveals that transcripts for five plant and one fungal phosphate transporter genes are contemporaneously present in arbusculated cells.</ArticleTitle><Pagination><MedlinePgn>1055-62</MedlinePgn></Pagination><Abstract><AbstractText>The establishment of a symbiotic interaction between plant roots and arbuscular mycorrhizal (AM) fungi requires both partners to undergo significant morphological and physiological modifications which eventually lead to reciprocal beneficial effects. Extensive changes in gene expression profiles recently have been described in transcriptomic studies that have analyzed the whole mycorrhizal root. However, because root colonization by AM fungi involves different cell types, a cell-specific gene expression pattern is likely to occur. We have applied the laser microdissection (LMD) technology to investigate expression profiles of both plant and fungal genes in Lycopersicon esculentum roots colonized by Glomus mosseae. A protocol to harvest arbuscule-containing cells from paraffin sections of mycorrhizal roots has been developed using a Leica AS LMD system. RNA of satisfactory quantity and quality has been extracted for molecular analysis. Transcripts for plant phosphate transporters (LePTs), selected as molecular markers for a functional symbiosis, have been detected by reverse-transcriptase polymerase chain reaction assays and associated to distinct cell types, leading to novel insights into the distribution of LePT mRNAs. In fact, the transcripts of the five phosphate transporters (PTs) have been detected contemporaneously in the same arbusculated cell population, unlike from the neighboring noncolonized cells. In addition, fungal H(+)ATPase (GmHA5) and phosphate transporter (GmosPT) mRNAs were found exclusively in arbusculated cells. The discovery that five plant and one fungal PT genes are consistently expressed inside the arbusculated cells provides a new scenario for plant-fungus nutrient exchanges.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Balestrini</LastName><ForeName>Raffaella</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Istituto Protezione Piante, CNR and Dipartimento di Biologia Vegetale, Università di Torino, Viale Mattioli, 25-10125 Torino, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gómez-Ariza</LastName><ForeName>Jorge</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Lanfranco</LastName><ForeName>Luisa</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Bonfante</LastName><ForeName>Paola</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D028061">Phosphate Transport Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012331">RNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007834" MajorTopicYN="Y">Lasers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042282" MajorTopicYN="N">Microdissection</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028061" MajorTopicYN="N">Phosphate Transport Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012331" MajorTopicYN="N">RNA, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>9</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>10</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>9</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17849708</ArticleId><ArticleId IdType="doi">10.1094/MPMI-20-9-1055</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MycorrhizaeV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002E43 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 002E43 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MycorrhizaeV1
|flux= Main
|étape= Curation
|type= RBID
|clé= pubmed:17849708
|texte= Laser microdissection reveals that transcripts for five plant and one fungal phosphate transporter genes are contemporaneously present in arbusculated cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:17849708" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \
| NlmPubMed2Wicri -a MycorrhizaeV1
| This area was generated with Dilib version V0.6.37. |  |