Endophytic fungal strains of Fusarium solani, from Apodytes dimidiata E. Mey. ex Arn (Icacinaceae) produce camptothecin, 10-hydroxycamptothecin and 9-methoxycamptothecin.
Identifieur interne : 002815 ( Main/Curation ); précédent : 002814; suivant : 002816Endophytic fungal strains of Fusarium solani, from Apodytes dimidiata E. Mey. ex Arn (Icacinaceae) produce camptothecin, 10-hydroxycamptothecin and 9-methoxycamptothecin.
Auteurs : S. Shweta [Inde] ; S. Zuehlke ; B T Ramesha ; V. Priti ; P. Mohana Kumar ; G. Ravikanth ; M. Spiteller ; R. Vasudeva ; R. Uma ShaankerSource :
- Phytochemistry [ 1873-3700 ] ; 2010.
Descripteurs français
- KwdFr :
- ADN ribosomique (MeSH), Antinéoplasiques d'origine végétale (isolement et purification), Arbres (MeSH), Camptothécine (analogues et dérivés), Camptothécine (isolement et purification), Fusarium (composition chimique), Fusarium (génétique), Fusarium (isolement et purification), Magnoliopsida (microbiologie), Mycorhizes (composition chimique), Séquence nucléotidique (MeSH).
- MESH :
- analogues et dérivés : Camptothécine.
- composition chimique : Fusarium, Mycorhizes.
- génétique : Fusarium.
- isolement et purification : Antinéoplasiques d'origine végétale, Camptothécine, Fusarium.
- microbiologie : Magnoliopsida.
- ADN ribosomique, Arbres, Séquence nucléotidique.
English descriptors
- KwdEn :
- Antineoplastic Agents, Phytogenic (isolation & purification), Base Sequence (MeSH), Camptothecin (analogs & derivatives), Camptothecin (isolation & purification), DNA, Ribosomal (MeSH), Fusarium (chemistry), Fusarium (genetics), Fusarium (isolation & purification), Magnoliopsida (microbiology), Mycorrhizae (chemistry), Trees (MeSH).
- MESH :
- chemical , analogs & derivatives : Camptothecin.
- chemical , isolation & purification : Antineoplastic Agents, Phytogenic, Camptothecin.
- chemistry : Fusarium, Mycorrhizae.
- genetics : Fusarium.
- isolation & purification : Fusarium.
- microbiology : Magnoliopsida.
- Base Sequence, DNA, Ribosomal, Trees.
Abstract
Camptothecin and 10-hydroxycamptothecin are two important precursors for the synthesis of the clinically useful anticancer drugs, topotecan and irinotecan. In recent years, efforts have been made to identify novel plant and endophytic fungal sources of camptothecin and 10-hydroxycamptothecin. In this study we have isolated endophytic fungi strains from Apodytes dimidiata (Icacinaceae), a medium sized tree from the Western Ghats, India. The fungi were identified as Fusarium solani using both ITS rDNA sequencing and spore morphology. Two strains, MTCC 9667 and MTCC 9668 were isolated, both of which produced camptothecin and 9-methoxycamptothecin in their mycelia; one of the strains, MTCC 9668 also produced 10-hydroxycamptothecin, though in small amounts. The yields of camptothecin in MTCC 9667 and MTCC 9668 were 37 and 53 microg/100g, respectively, after 4 days of incubation in broth culture. The yields of 10-hydroxycamptothecin and 9-methoxycamptothecin in MTCC 9668 were 8.2 and 44.9 microg/100g, respectively. Further research in optimizing the culture conditions of these fungal strains might permit their application for the production of camptothecin and 10-hydroxycamptothecin.
DOI: 10.1016/j.phytochem.2009.09.030
PubMed: 19863979
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Zuehlke</name></author><author><name sortKey="Ramesha, B T" sort="Ramesha, B T" uniqKey="Ramesha B" first="B T" last="Ramesha">B T Ramesha</name></author><author><name sortKey="Priti, V" sort="Priti, V" uniqKey="Priti V" first="V" last="Priti">V. Priti</name></author><author><name sortKey="Mohana Kumar, P" sort="Mohana Kumar, P" uniqKey="Mohana Kumar P" first="P" last="Mohana Kumar">P. Mohana Kumar</name></author><author><name sortKey="Ravikanth, G" sort="Ravikanth, G" uniqKey="Ravikanth G" first="G" last="Ravikanth">G. Ravikanth</name></author><author><name sortKey="Spiteller, M" sort="Spiteller, M" uniqKey="Spiteller M" first="M" last="Spiteller">M. Spiteller</name></author><author><name sortKey="Vasudeva, R" sort="Vasudeva, R" uniqKey="Vasudeva R" first="R" last="Vasudeva">R. Vasudeva</name></author><author><name sortKey="Uma Shaanker, R" sort="Uma Shaanker, R" uniqKey="Uma Shaanker R" first="R" last="Uma Shaanker">R. 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Shweta</name><affiliation wicri:level="1"><nlm:affiliation>School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bangalore 560065, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bangalore 560065</wicri:regionArea></affiliation></author><author><name sortKey="Zuehlke, S" sort="Zuehlke, S" uniqKey="Zuehlke S" first="S" last="Zuehlke">S. Zuehlke</name></author><author><name sortKey="Ramesha, B T" sort="Ramesha, B T" uniqKey="Ramesha B" first="B T" last="Ramesha">B T Ramesha</name></author><author><name sortKey="Priti, V" sort="Priti, V" uniqKey="Priti V" first="V" last="Priti">V. Priti</name></author><author><name sortKey="Mohana Kumar, P" sort="Mohana Kumar, P" uniqKey="Mohana Kumar P" first="P" last="Mohana Kumar">P. Mohana Kumar</name></author><author><name sortKey="Ravikanth, G" sort="Ravikanth, G" uniqKey="Ravikanth G" first="G" last="Ravikanth">G. Ravikanth</name></author><author><name sortKey="Spiteller, M" sort="Spiteller, M" uniqKey="Spiteller M" first="M" last="Spiteller">M. Spiteller</name></author><author><name sortKey="Vasudeva, R" sort="Vasudeva, R" uniqKey="Vasudeva R" first="R" last="Vasudeva">R. Vasudeva</name></author><author><name sortKey="Uma Shaanker, R" sort="Uma Shaanker, R" uniqKey="Uma Shaanker R" first="R" last="Uma Shaanker">R. Uma Shaanker</name></author></analytic><series><title level="j">Phytochemistry</title><idno type="eISSN">1873-3700</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antineoplastic Agents, Phytogenic (isolation & purification)</term><term>Base Sequence (MeSH)</term><term>Camptothecin (analogs & derivatives)</term><term>Camptothecin (isolation & purification)</term><term>DNA, Ribosomal (MeSH)</term><term>Fusarium (chemistry)</term><term>Fusarium (genetics)</term><term>Fusarium (isolation & purification)</term><term>Magnoliopsida (microbiology)</term><term>Mycorrhizae (chemistry)</term><term>Trees (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ribosomique (MeSH)</term><term>Antinéoplasiques d'origine végétale (isolement et purification)</term><term>Arbres (MeSH)</term><term>Camptothécine (analogues et dérivés)</term><term>Camptothécine (isolement et purification)</term><term>Fusarium (composition chimique)</term><term>Fusarium (génétique)</term><term>Fusarium (isolement et purification)</term><term>Magnoliopsida (microbiologie)</term><term>Mycorhizes (composition chimique)</term><term>Séquence nucléotidique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Camptothecin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antineoplastic Agents, Phytogenic</term><term>Camptothecin</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Camptothécine</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Fusarium</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Fusarium</term><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antinéoplasiques d'origine végétale</term><term>Camptothécine</term><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Magnoliopsida</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Magnoliopsida</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>DNA, Ribosomal</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN ribosomique</term><term>Arbres</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Camptothecin and 10-hydroxycamptothecin are two important precursors for the synthesis of the clinically useful anticancer drugs, topotecan and irinotecan. In recent years, efforts have been made to identify novel plant and endophytic fungal sources of camptothecin and 10-hydroxycamptothecin. In this study we have isolated endophytic fungi strains from Apodytes dimidiata (Icacinaceae), a medium sized tree from the Western Ghats, India. The fungi were identified as Fusarium solani using both ITS rDNA sequencing and spore morphology. Two strains, MTCC 9667 and MTCC 9668 were isolated, both of which produced camptothecin and 9-methoxycamptothecin in their mycelia; one of the strains, MTCC 9668 also produced 10-hydroxycamptothecin, though in small amounts. The yields of camptothecin in MTCC 9667 and MTCC 9668 were 37 and 53 microg/100g, respectively, after 4 days of incubation in broth culture. The yields of 10-hydroxycamptothecin and 9-methoxycamptothecin in MTCC 9668 were 8.2 and 44.9 microg/100g, respectively. Further research in optimizing the culture conditions of these fungal strains might permit their application for the production of camptothecin and 10-hydroxycamptothecin.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19863979</PMID><DateCompleted><Year>2010</Year><Month>07</Month><Day>27</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-3700</ISSN><JournalIssue CitedMedium="Internet"><Volume>71</Volume><Issue>1</Issue><PubDate><Year>2010</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Phytochemistry</Title><ISOAbbreviation>Phytochemistry</ISOAbbreviation></Journal><ArticleTitle>Endophytic fungal strains of Fusarium solani, from Apodytes dimidiata E. Mey. ex Arn (Icacinaceae) produce camptothecin, 10-hydroxycamptothecin and 9-methoxycamptothecin.</ArticleTitle><Pagination><MedlinePgn>117-22</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.phytochem.2009.09.030</ELocationID><Abstract><AbstractText>Camptothecin and 10-hydroxycamptothecin are two important precursors for the synthesis of the clinically useful anticancer drugs, topotecan and irinotecan. In recent years, efforts have been made to identify novel plant and endophytic fungal sources of camptothecin and 10-hydroxycamptothecin. In this study we have isolated endophytic fungi strains from Apodytes dimidiata (Icacinaceae), a medium sized tree from the Western Ghats, India. The fungi were identified as Fusarium solani using both ITS rDNA sequencing and spore morphology. Two strains, MTCC 9667 and MTCC 9668 were isolated, both of which produced camptothecin and 9-methoxycamptothecin in their mycelia; one of the strains, MTCC 9668 also produced 10-hydroxycamptothecin, though in small amounts. The yields of camptothecin in MTCC 9667 and MTCC 9668 were 37 and 53 microg/100g, respectively, after 4 days of incubation in broth culture. The yields of 10-hydroxycamptothecin and 9-methoxycamptothecin in MTCC 9668 were 8.2 and 44.9 microg/100g, respectively. Further research in optimizing the culture conditions of these fungal strains might permit their application for the production of camptothecin and 10-hydroxycamptothecin.</AbstractText><CopyrightInformation>2009. Published by Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shweta</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bangalore 560065, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zuehlke</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Ramesha</LastName><ForeName>B T</ForeName><Initials>BT</Initials></Author><Author ValidYN="Y"><LastName>Priti</LastName><ForeName>V</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Mohana Kumar</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Ravikanth</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Spiteller</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Vasudeva</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Uma Shaanker</LastName><ForeName>R</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>10</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochemistry</MedlineTA><NlmUniqueID>0151434</NlmUniqueID><ISSNLinking>0031-9422</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000972">Antineoplastic Agents, Phytogenic</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004275">DNA, Ribosomal</NameOfSubstance></Chemical><Chemical><RegistryNumber>19685-10-0</RegistryNumber><NameOfSubstance UI="C025724">9-methoxycamptothecin</NameOfSubstance></Chemical><Chemical><RegistryNumber>9Z01632KRV</RegistryNumber><NameOfSubstance UI="C028098">10-hydroxycamptothecin</NameOfSubstance></Chemical><Chemical><RegistryNumber>XT3Z54Z28A</RegistryNumber><NameOfSubstance UI="D002166">Camptothecin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000972" MajorTopicYN="N">Antineoplastic Agents, Phytogenic</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002166" MajorTopicYN="N">Camptothecin</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="Y">analogs & derivatives</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004275" MajorTopicYN="N">DNA, Ribosomal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005670" MajorTopicYN="N">Fusarium</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019684" MajorTopicYN="Y">Magnoliopsida</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>02</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2009</Year><Month>08</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>09</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>10</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>10</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>7</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19863979</ArticleId><ArticleId IdType="pii">S0031-9422(09)00420-8</ArticleId><ArticleId IdType="doi">10.1016/j.phytochem.2009.09.030</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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