Phylogenetic species delimitation in ectomycorrhizal fungi and implications for barcoding: the case of the Tricholoma scalpturatum complex (Basidiomycota).
Identifieur interne : 002507 ( Main/Curation ); précédent : 002506; suivant : 002508Phylogenetic species delimitation in ectomycorrhizal fungi and implications for barcoding: the case of the Tricholoma scalpturatum complex (Basidiomycota).
Auteurs : Patricia Jargeat [France] ; Florent Martos ; Fabian Carriconde ; Hervé Gryta ; Pierre-Arthur Moreau ; Monique GardesSource :
- Molecular ecology [ 1365-294X ] ; 2010.
Descripteurs français
- KwdFr :
- ADN fongique (génétique), Analyse de séquence d'ADN (MeSH), Codage à barres de l'ADN pour la taxonomie (MeSH), Espaceur de l'ADN ribosomique (génétique), Europe (MeSH), Haplotypes (MeSH), Mycorhizes (classification), Mycorhizes (génétique), Noyau de la cellule (génétique), Phylogenèse (MeSH), Tricholoma (classification), Tricholoma (génétique), Variation génétique (MeSH).
- MESH :
English descriptors
- KwdEn :
- Cell Nucleus (genetics), DNA Barcoding, Taxonomic (MeSH), DNA, Fungal (genetics), DNA, Ribosomal Spacer (genetics), Europe (MeSH), Genetic Variation (MeSH), Haplotypes (MeSH), Mycorrhizae (classification), Mycorrhizae (genetics), Phylogeny (MeSH), Sequence Analysis, DNA (MeSH), Tricholoma (classification), Tricholoma (genetics).
- MESH :
- chemical , genetics : DNA, Fungal, DNA, Ribosomal Spacer.
- geographic : Europe.
- classification : Mycorrhizae, Tricholoma.
- genetics : Cell Nucleus, Mycorrhizae, Tricholoma.
- DNA Barcoding, Taxonomic, Genetic Variation, Haplotypes, Phylogeny, Sequence Analysis, DNA.
Abstract
Population studies have revealed that the fungal ectomycorrhizal morphospecies Tricholoma scalpturatum consists of at least two genetically distinct groups that occur sympatrically in several geographical areas. This discovery prompted us to examine species boundaries and relationships between members formerly assigned to T. scalpturatum and allied taxa using phylogenetic analyses. Sequence data were obtained from three nuclear DNA regions [internal transcribed spacer (ITS), gpd and tef], from 101 carpophores collected over a large geographical range in Western Europe, and some reference sequences from public databases. The ITS was also tested for its applicability as DNA barcode for species delimitation. Four highly supported phylogenetic clades were detected. The two previously detected genetic groups of T. scalpturatum were assigned to the phylospecies Tricholoma argyraceum and T. scalpturatum. The two remaining clades were referred to as Tricholoma cingulatum and Tricholoma inocybeoides. Unexpectedly, T. cingulatum showed an accelerated rate of evolution that we attributed to narrow host specialization. This study also reveals recombinant ITS sequences in T. inocybeoides, suggesting a hybrid origin. The ITS was a useful tool for the determination of species boundaries: the mean value of intraspecific genetic distances in the entire ITS region (including 5.8S rDNA) was <0.2%, whereas interspecific divergence estimates ranged from 1.78% to 4.22%. Apart from giving insights into the evolution of the T. scalpturatum complex, this study contributes to the establishment of a library of taxonomically verified voucher specimens, an a posteriori correlation between phenotype and genotype, and DNA barcoding of ectomycorrhizal fungi.
DOI: 10.1111/j.1365-294X.2010.04863.x
PubMed: 21044190
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118 route de Narbonne, F-31062 Toulouse, France. jargeat@cict.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique); 118 route de Narbonne, F-31062 Toulouse</wicri:regionArea></affiliation></author><author><name sortKey="Martos, Florent" sort="Martos, Florent" uniqKey="Martos F" first="Florent" last="Martos">Florent Martos</name></author><author><name sortKey="Carriconde, Fabian" sort="Carriconde, Fabian" uniqKey="Carriconde F" first="Fabian" last="Carriconde">Fabian Carriconde</name></author><author><name sortKey="Gryta, Herve" sort="Gryta, Herve" uniqKey="Gryta H" first="Hervé" last="Gryta">Hervé Gryta</name></author><author><name sortKey="Moreau, Pierre Arthur" sort="Moreau, Pierre Arthur" uniqKey="Moreau P" first="Pierre-Arthur" last="Moreau">Pierre-Arthur Moreau</name></author><author><name sortKey="Gardes, Monique" sort="Gardes, Monique" uniqKey="Gardes M" first="Monique" last="Gardes">Monique Gardes</name></author></analytic><series><title level="j">Molecular ecology</title><idno type="eISSN">1365-294X</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Nucleus (genetics)</term><term>DNA Barcoding, Taxonomic (MeSH)</term><term>DNA, Fungal (genetics)</term><term>DNA, Ribosomal Spacer (genetics)</term><term>Europe (MeSH)</term><term>Genetic Variation (MeSH)</term><term>Haplotypes (MeSH)</term><term>Mycorrhizae (classification)</term><term>Mycorrhizae (genetics)</term><term>Phylogeny (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Tricholoma (classification)</term><term>Tricholoma (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Codage à barres de l'ADN pour la taxonomie (MeSH)</term><term>Espaceur de l'ADN ribosomique (génétique)</term><term>Europe (MeSH)</term><term>Haplotypes (MeSH)</term><term>Mycorhizes (classification)</term><term>Mycorhizes (génétique)</term><term>Noyau de la cellule (génétique)</term><term>Phylogenèse (MeSH)</term><term>Tricholoma (classification)</term><term>Tricholoma (génétique)</term><term>Variation génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Fungal</term><term>DNA, Ribosomal Spacer</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Europe</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Mycorrhizae</term><term>Tricholoma</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Nucleus</term><term>Mycorrhizae</term><term>Tricholoma</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term><term>Espaceur de l'ADN ribosomique</term><term>Mycorhizes</term><term>Noyau de la cellule</term><term>Tricholoma</term></keywords><keywords scheme="MESH" xml:lang="en"><term>DNA Barcoding, Taxonomic</term><term>Genetic Variation</term><term>Haplotypes</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Codage à barres de l'ADN pour la taxonomie</term><term>Europe</term><term>Haplotypes</term><term>Phylogenèse</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Population studies have revealed that the fungal ectomycorrhizal morphospecies Tricholoma scalpturatum consists of at least two genetically distinct groups that occur sympatrically in several geographical areas. This discovery prompted us to examine species boundaries and relationships between members formerly assigned to T. scalpturatum and allied taxa using phylogenetic analyses. Sequence data were obtained from three nuclear DNA regions [internal transcribed spacer (ITS), gpd and tef], from 101 carpophores collected over a large geographical range in Western Europe, and some reference sequences from public databases. The ITS was also tested for its applicability as DNA barcode for species delimitation. Four highly supported phylogenetic clades were detected. The two previously detected genetic groups of T. scalpturatum were assigned to the phylospecies Tricholoma argyraceum and T. scalpturatum. The two remaining clades were referred to as Tricholoma cingulatum and Tricholoma inocybeoides. Unexpectedly, T. cingulatum showed an accelerated rate of evolution that we attributed to narrow host specialization. This study also reveals recombinant ITS sequences in T. inocybeoides, suggesting a hybrid origin. The ITS was a useful tool for the determination of species boundaries: the mean value of intraspecific genetic distances in the entire ITS region (including 5.8S rDNA) was <0.2%, whereas interspecific divergence estimates ranged from 1.78% to 4.22%. Apart from giving insights into the evolution of the T. scalpturatum complex, this study contributes to the establishment of a library of taxonomically verified voucher specimens, an a posteriori correlation between phenotype and genotype, and DNA barcoding of ectomycorrhizal fungi.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21044190</PMID><DateCompleted><Year>2011</Year><Month>02</Month><Day>17</Day></DateCompleted><DateRevised><Year>2010</Year><Month>11</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-294X</ISSN><JournalIssue CitedMedium="Internet"><Volume>19</Volume><Issue>23</Issue><PubDate><Year>2010</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Molecular ecology</Title><ISOAbbreviation>Mol Ecol</ISOAbbreviation></Journal><ArticleTitle>Phylogenetic species delimitation in ectomycorrhizal fungi and implications for barcoding: the case of the Tricholoma scalpturatum complex (Basidiomycota).</ArticleTitle><Pagination><MedlinePgn>5216-30</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1365-294X.2010.04863.x</ELocationID><Abstract><AbstractText>Population studies have revealed that the fungal ectomycorrhizal morphospecies Tricholoma scalpturatum consists of at least two genetically distinct groups that occur sympatrically in several geographical areas. This discovery prompted us to examine species boundaries and relationships between members formerly assigned to T. scalpturatum and allied taxa using phylogenetic analyses. Sequence data were obtained from three nuclear DNA regions [internal transcribed spacer (ITS), gpd and tef], from 101 carpophores collected over a large geographical range in Western Europe, and some reference sequences from public databases. The ITS was also tested for its applicability as DNA barcode for species delimitation. Four highly supported phylogenetic clades were detected. The two previously detected genetic groups of T. scalpturatum were assigned to the phylospecies Tricholoma argyraceum and T. scalpturatum. The two remaining clades were referred to as Tricholoma cingulatum and Tricholoma inocybeoides. Unexpectedly, T. cingulatum showed an accelerated rate of evolution that we attributed to narrow host specialization. This study also reveals recombinant ITS sequences in T. inocybeoides, suggesting a hybrid origin. The ITS was a useful tool for the determination of species boundaries: the mean value of intraspecific genetic distances in the entire ITS region (including 5.8S rDNA) was <0.2%, whereas interspecific divergence estimates ranged from 1.78% to 4.22%. Apart from giving insights into the evolution of the T. scalpturatum complex, this study contributes to the establishment of a library of taxonomically verified voucher specimens, an a posteriori correlation between phenotype and genotype, and DNA barcoding of ectomycorrhizal fungi.</AbstractText><CopyrightInformation>© 2010 Blackwell Publishing Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jargeat</LastName><ForeName>Patricia</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Université de Toulouse, UPS, UMR5174 EDB (Laboratoire Evolution et Diversité Biologique); 118 route de Narbonne, F-31062 Toulouse, France. jargeat@cict.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martos</LastName><ForeName>Florent</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Carriconde</LastName><ForeName>Fabian</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Gryta</LastName><ForeName>Hervé</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Moreau</LastName><ForeName>Pierre-Arthur</ForeName><Initials>PA</Initials></Author><Author ValidYN="Y"><LastName>Gardes</LastName><ForeName>Monique</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>11</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Ecol</MedlineTA><NlmUniqueID>9214478</NlmUniqueID><ISSNLinking>0962-1083</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021903">DNA, Ribosomal Spacer</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058893" MajorTopicYN="N">DNA Barcoding, Taxonomic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021903" MajorTopicYN="N">DNA, Ribosomal Spacer</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005060" MajorTopicYN="N" Type="Geographic">Europe</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="N">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006239" MajorTopicYN="N">Haplotypes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="Y">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055439" MajorTopicYN="N">Tricholoma</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>11</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>11</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>2</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21044190</ArticleId><ArticleId IdType="doi">10.1111/j.1365-294X.2010.04863.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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