[Molecular diversity of arbuscular mycorrhizal fungi in wild and cultured Gynostemma pentaphyllum roots in Xishuangbanna, Southwest China].
Identifieur interne : 001982 ( Main/Curation ); précédent : 001981; suivant : 001983[Molecular diversity of arbuscular mycorrhizal fungi in wild and cultured Gynostemma pentaphyllum roots in Xishuangbanna, Southwest China].
Auteurs : Li-Si Zhou [République populaire de Chine] ; Shun-Xing Guo [République populaire de Chine]Source :
- Ying yong sheng tai xue bao = The journal of applied ecology [ 1001-9332 ] ; 2013.
Descripteurs français
- KwdFr :
- ADN fongique (analyse), Biodiversité (MeSH), Chine (MeSH), Gynostemma (croissance et développement), Gynostemma (microbiologie), Microbiologie du sol (MeSH), Mycorhizes (classification), Mycorhizes (génétique), Polymorphisme de restriction (MeSH), Racines de plante (microbiologie), Réaction de polymérisation en chaîne (MeSH), Symbiose (MeSH).
- MESH :
- analyse : ADN fongique.
- croissance et développement : Gynostemma.
- génétique : Mycorhizes.
- microbiologie : Gynostemma, Racines de plante.
- Biodiversité, Chine, Microbiologie du sol, Mycorhizes, Polymorphisme de restriction, Réaction de polymérisation en chaîne, Symbiose.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- Biodiversity (MeSH), China (MeSH), DNA, Fungal (analysis), Gynostemma (growth & development), Gynostemma (microbiology), Mycorrhizae (classification), Mycorrhizae (genetics), Plant Roots (microbiology), Polymerase Chain Reaction (MeSH), Polymorphism, Restriction Fragment Length (MeSH), Soil Microbiology (MeSH), Symbiosis (MeSH).
- MESH :
- chemical , analysis : DNA, Fungal.
- geographic : China.
- classification : Mycorrhizae.
- genetics : Mycorrhizae.
- growth & development : Gynostemma.
- microbiology : Gynostemma, Plant Roots.
- Biodiversity, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Soil Microbiology, Symbiosis.
Abstract
By using nested-PCR, DNA cloning, and sequencing techniques, this paper studied the diversity of the community structure of arbuscular mycorrhizal fungi (AMF) in wild and cultured Gynostemma pentaphyllum roots. A total of 551 clones containing 18S rDNA genes of AMF were obtained from the roots. After the analysis of the restriction fragment length polymorphism, 100 different RFLP types were obtained, which were further divided into 20 AMF phylotypes belonging to seven families. The comparison of the sequences of 20 AMF phylotypes with the GenBank database showed that there were 5 AMF phylotypes having high similarity to the sequences of reported AMF species Glomus viscosum, Claroideoglomus etunicatum, Racocetra tropicana, Acaulospora spinosa, and Acaulospora mellea, respectively. These sequences were then assessed for the similarities against the MaarjAM database, and 12 phylotypes showed high similarity to the corresponding molecular virtual taxa, of which, 7 phylotypes were not obtained by the morphological identification of soil asexual spores. Statistical analysis indicated that there were significant differences in the AMF community between wild and cultured G. pentaphyllum roots. The analysis of relative abundance data indicated that Glo-2, Amb-1, and Para-1 were the dominant phylotypes in wild G. pentaphyllum roots, while Glo-3, Glo-8, Glo-10, and Div-1 were the prevalent phylotypes in cultured ones. Claroideoglomeraceae and Ambisporaceae were only detected in wild G. pentaphyllum roots, and Diversisporaceae was only identified in cultured ones.
PubMed: 24417107
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roots in Xishuangbanna, Southwest China].</title><author><name sortKey="Zhou, Li Si" sort="Zhou, Li Si" uniqKey="Zhou L" first="Li-Si" last="Zhou">Li-Si Zhou</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. zls921203@sina.com</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193</wicri:regionArea></affiliation></author><author><name sortKey="Guo, Shun Xing" sort="Guo, Shun Xing" uniqKey="Guo S" first="Shun-Xing" last="Guo">Shun-Xing Guo</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193</wicri:regionArea></affiliation></author></analytic><series><title level="j">Ying yong sheng tai xue bao = The journal of applied ecology</title><idno type="ISSN">1001-9332</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodiversity (MeSH)</term><term>China (MeSH)</term><term>DNA, Fungal (analysis)</term><term>Gynostemma (growth & development)</term><term>Gynostemma (microbiology)</term><term>Mycorrhizae (classification)</term><term>Mycorrhizae (genetics)</term><term>Plant Roots (microbiology)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Polymorphism, Restriction Fragment Length (MeSH)</term><term>Soil Microbiology (MeSH)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (analyse)</term><term>Biodiversité (MeSH)</term><term>Chine (MeSH)</term><term>Gynostemma (croissance et développement)</term><term>Gynostemma (microbiologie)</term><term>Microbiologie du sol (MeSH)</term><term>Mycorhizes (classification)</term><term>Mycorhizes (génétique)</term><term>Polymorphisme de restriction (MeSH)</term><term>Racines de plante (microbiologie)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Symbiose (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Fungal</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN fongique</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Gynostemma</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Gynostemma</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Gynostemma</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Gynostemma</term><term>Plant Roots</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodiversity</term><term>Polymerase Chain Reaction</term><term>Polymorphism, Restriction Fragment Length</term><term>Soil Microbiology</term><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biodiversité</term><term>Chine</term><term>Microbiologie du sol</term><term>Mycorhizes</term><term>Polymorphisme de restriction</term><term>Réaction de polymérisation en chaîne</term><term>Symbiose</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">By using nested-PCR, DNA cloning, and sequencing techniques, this paper studied the diversity of the community structure of arbuscular mycorrhizal fungi (AMF) in wild and cultured Gynostemma pentaphyllum roots. A total of 551 clones containing 18S rDNA genes of AMF were obtained from the roots. After the analysis of the restriction fragment length polymorphism, 100 different RFLP types were obtained, which were further divided into 20 AMF phylotypes belonging to seven families. The comparison of the sequences of 20 AMF phylotypes with the GenBank database showed that there were 5 AMF phylotypes having high similarity to the sequences of reported AMF species Glomus viscosum, Claroideoglomus etunicatum, Racocetra tropicana, Acaulospora spinosa, and Acaulospora mellea, respectively. These sequences were then assessed for the similarities against the MaarjAM database, and 12 phylotypes showed high similarity to the corresponding molecular virtual taxa, of which, 7 phylotypes were not obtained by the morphological identification of soil asexual spores. Statistical analysis indicated that there were significant differences in the AMF community between wild and cultured G. pentaphyllum roots. The analysis of relative abundance data indicated that Glo-2, Amb-1, and Para-1 were the dominant phylotypes in wild G. pentaphyllum roots, while Glo-3, Glo-8, Glo-10, and Div-1 were the prevalent phylotypes in cultured ones. Claroideoglomeraceae and Ambisporaceae were only detected in wild G. pentaphyllum roots, and Diversisporaceae was only identified in cultured ones.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24417107</PMID><DateCompleted><Year>2015</Year><Month>03</Month><Day>21</Day></DateCompleted><DateRevised><Year>2016</Year><Month>10</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1001-9332</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>9</Issue><PubDate><Year>2013</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Ying yong sheng tai xue bao = The journal of applied ecology</Title><ISOAbbreviation>Ying Yong Sheng Tai Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Molecular diversity of arbuscular mycorrhizal fungi in wild and cultured Gynostemma pentaphyllum roots in Xishuangbanna, Southwest China].</ArticleTitle><Pagination><MedlinePgn>2503-10</MedlinePgn></Pagination><Abstract><AbstractText>By using nested-PCR, DNA cloning, and sequencing techniques, this paper studied the diversity of the community structure of arbuscular mycorrhizal fungi (AMF) in wild and cultured Gynostemma pentaphyllum roots. A total of 551 clones containing 18S rDNA genes of AMF were obtained from the roots. After the analysis of the restriction fragment length polymorphism, 100 different RFLP types were obtained, which were further divided into 20 AMF phylotypes belonging to seven families. The comparison of the sequences of 20 AMF phylotypes with the GenBank database showed that there were 5 AMF phylotypes having high similarity to the sequences of reported AMF species Glomus viscosum, Claroideoglomus etunicatum, Racocetra tropicana, Acaulospora spinosa, and Acaulospora mellea, respectively. These sequences were then assessed for the similarities against the MaarjAM database, and 12 phylotypes showed high similarity to the corresponding molecular virtual taxa, of which, 7 phylotypes were not obtained by the morphological identification of soil asexual spores. Statistical analysis indicated that there were significant differences in the AMF community between wild and cultured G. pentaphyllum roots. The analysis of relative abundance data indicated that Glo-2, Amb-1, and Para-1 were the dominant phylotypes in wild G. pentaphyllum roots, while Glo-3, Glo-8, Glo-10, and Div-1 were the prevalent phylotypes in cultured ones. Claroideoglomeraceae and Ambisporaceae were only detected in wild G. pentaphyllum roots, and Diversisporaceae was only identified in cultured ones.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Li-Si</ForeName><Initials>LS</Initials><AffiliationInfo><Affiliation>Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. zls921203@sina.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Shun-Xing</ForeName><Initials>SX</Initials><AffiliationInfo><Affiliation>Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Ying Yong Sheng Tai Xue Bao</MedlineTA><NlmUniqueID>9425159</NlmUniqueID><ISSNLinking>1001-9332</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D044822" MajorTopicYN="N">Biodiversity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002681" MajorTopicYN="N" Type="Geographic">China</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029774" MajorTopicYN="N">Gynostemma</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="Y">Mycorrhizae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012150" MajorTopicYN="N">Polymorphism, Restriction Fragment Length</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="N">Soil Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>1</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>1</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>3</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24417107</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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