Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Cordoba (Argentina).
Identifieur interne : 001353 ( Main/Curation ); précédent : 001352; suivant : 001354Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Cordoba (Argentina).
Auteurs : Agustina Fernández Di Pardo ; Viviana M. Chiocchio ; Viviana Barrera ; Roxana P. Colombo ; Alicia E. Martinez ; Laura Gasoni ; Alicia M. GodeasSource :
- Revista de biologia tropical [ 0034-7744 ] ; 2015.
Descripteurs français
- KwdFr :
- ADN fongique (MeSH), ADN ribosomique (MeSH), Argentine (MeSH), Mycorhizes (classification), Mycorhizes (croissance et développement), Mycorhizes (génétique), Orchidaceae (classification), Orchidaceae (croissance et développement), Orchidaceae (microbiologie), Phylogenèse (MeSH), Racines de plante (microbiologie), Symbiose (MeSH).
- MESH :
- croissance et développement : Mycorhizes, Orchidaceae.
- génétique : Mycorhizes.
- microbiologie : Orchidaceae, Racines de plante.
- ADN fongique, ADN ribosomique, Argentine, Mycorhizes, Orchidaceae, Phylogenèse, Symbiose.
- Wicri :
- geographic : Argentine.
English descriptors
- KwdEn :
- Argentina (MeSH), DNA, Fungal (MeSH), DNA, Ribosomal (MeSH), Mycorrhizae (classification), Mycorrhizae (genetics), Mycorrhizae (growth & development), Orchidaceae (classification), Orchidaceae (growth & development), Orchidaceae (microbiology), Phylogeny (MeSH), Plant Roots (microbiology), Symbiosis (MeSH).
- MESH :
- chemical : DNA, Fungal, DNA, Ribosomal.
- geographic : Argentina.
- classification : Mycorrhizae, Orchidaceae.
- genetics : Mycorrhizae.
- growth & development : Mycorrhizae, Orchidaceae.
- microbiology : Orchidaceae, Plant Roots.
- Phylogeny, Symbiosis.
Abstract
Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Cordoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizosphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton presence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25 degrees C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained the ITS1-5.8s-ITS4 region that was amplified using primers ITSI and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium. Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the asso- ciation of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina.
DOI: 10.15517/rbt.v63i1.14226
PubMed: 26299131
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Martinez</name></author><author><name sortKey="Gasoni, Laura" sort="Gasoni, Laura" uniqKey="Gasoni L" first="Laura" last="Gasoni">Laura Gasoni</name></author><author><name sortKey="Godeas, Alicia M" sort="Godeas, Alicia M" uniqKey="Godeas A" first="Alicia M" last="Godeas">Alicia M. 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Godeas</name></author></analytic><series><title level="j">Revista de biologia tropical</title><idno type="ISSN">0034-7744</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Argentina (MeSH)</term><term>DNA, Fungal (MeSH)</term><term>DNA, Ribosomal (MeSH)</term><term>Mycorrhizae (classification)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (growth & development)</term><term>Orchidaceae (classification)</term><term>Orchidaceae (growth & development)</term><term>Orchidaceae (microbiology)</term><term>Phylogeny (MeSH)</term><term>Plant Roots (microbiology)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (MeSH)</term><term>ADN ribosomique (MeSH)</term><term>Argentine (MeSH)</term><term>Mycorhizes (classification)</term><term>Mycorhizes (croissance et développement)</term><term>Mycorhizes (génétique)</term><term>Orchidaceae (classification)</term><term>Orchidaceae (croissance et développement)</term><term>Orchidaceae (microbiologie)</term><term>Phylogenèse (MeSH)</term><term>Racines de plante (microbiologie)</term><term>Symbiose (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Fungal</term><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Argentina</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Mycorrhizae</term><term>Orchidaceae</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Mycorhizes</term><term>Orchidaceae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Mycorrhizae</term><term>Orchidaceae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Orchidaceae</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Orchidaceae</term><term>Plant Roots</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Phylogeny</term><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN fongique</term><term>ADN ribosomique</term><term>Argentine</term><term>Mycorhizes</term><term>Orchidaceae</term><term>Phylogenèse</term><term>Symbiose</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Argentine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Cordoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizosphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton presence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25 degrees C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained the ITS1-5.8s-ITS4 region that was amplified using primers ITSI and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium. Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the asso- ciation of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26299131</PMID><DateCompleted><Year>2015</Year><Month>10</Month><Day>01</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0034-7744</ISSN><JournalIssue CitedMedium="Print"><Volume>63</Volume><Issue>1</Issue><PubDate><Year>2015</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Revista de biologia tropical</Title><ISOAbbreviation>Rev Biol Trop</ISOAbbreviation></Journal><ArticleTitle>Mycorrhizal fungi isolated from native terrestrial orchids of pristine regions in Cordoba (Argentina).</ArticleTitle><Pagination><MedlinePgn>275-83</MedlinePgn></Pagination><Abstract><AbstractText>Orchidaceae is a highly dependent group on the Rhizoctonia complex that includes Ceratorhiza, Moniliopsis, Epulorhiza and Rhizoctonia, for seed germination and the development of new orchid plants. Thus, the isolation and identification of orchid mycorrhizal fungi are important to understand the orchid-fungus relationship, which can lead to the development of efficient conservation strategies by in vivo germination of seeds from endangered orchid plants. The aim of our work was to isolate and characterize the different mycorrhizal fungi found in roots of terrestrial orchids from Cordoba (Argentina), and, to learn about the natural habit and fungal associations in the Chaco Serrano woodland pristine region. In this study, bloomed orchid root and rhizosphere soil samples were obtained in two times from Valle de Punilla during spring of 2007; samples were kept in plastic bags until processed within 48 hours, and mycorrhizal condition confirmed assessing peloton presence. A total of 23 isolates of the orchideous mycorrhizal Rhizoctonia complex were obtained. The isolates were studied based on morphological characters and ITS-rDNA sequences. Morphological characteristics as color of colonies, texture, growth rate, hyphal diameter and length and presence of sclerotia were observed on culture media. To define the number of nuclei per cell, the isolates were grown in Petri dishes containing water-agar (WA) for three days at 25 degrees C and stained with Safranine-O solution. The mycorrhizal fungi were grouped into binucleate (MSGib, 10 isolates) and multinucleate (MSGim, 13 isolates) based on morphological characteristics of the colonies. We obtained the ITS1-5.8s-ITS4 region that was amplified using primers ITSI and ITS4. Based on DNA sequencing, isolates Q23 and Q29 were found to be related to species of Ceratobasidium. Isolates Q24 and Q4 were related to the binucleated anastomosis group AG-C of Rhizoctonia sp. The rest of the isolates grouped in the Ceratobasidium clade without grouping. From our knowledge this is the first report of the asso- ciation of the AG-C testers with terrestrial orchids. A high specificity was observed in the symbiotic relationship. As the mycorrhizal fungal isolates were obtained from native orchids, they could be incorporated in conservation programes of endangered orchids in Argentina.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fernández Di Pardo</LastName><ForeName>Agustina</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Chiocchio</LastName><ForeName>Viviana M</ForeName><Initials>VM</Initials></Author><Author ValidYN="Y"><LastName>Barrera</LastName><ForeName>Viviana</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Colombo</LastName><ForeName>Roxana P</ForeName><Initials>RP</Initials></Author><Author ValidYN="Y"><LastName>Martinez</LastName><ForeName>Alicia E</ForeName><Initials>AE</Initials></Author><Author ValidYN="Y"><LastName>Gasoni</LastName><ForeName>Laura</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Godeas</LastName><ForeName>Alicia M</ForeName><Initials>AM</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Costa Rica</Country><MedlineTA>Rev Biol Trop</MedlineTA><NlmUniqueID>0404267</NlmUniqueID><ISSNLinking>0034-7744</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004275">DNA, Ribosomal</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001118" MajorTopicYN="N" Type="Geographic">Argentina</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004275" MajorTopicYN="N">DNA, Ribosomal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029595" MajorTopicYN="N">Orchidaceae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>8</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>8</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>10</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26299131</ArticleId><ArticleId IdType="doi">10.15517/rbt.v63i1.14226</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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