Cost-efficient production of in vitro Rhizophagus irregularis.
Identifieur interne : 000D56 ( Main/Curation ); précédent : 000D55; suivant : 000D57Cost-efficient production of in vitro Rhizophagus irregularis.
Auteurs : Pawel Rosikiewicz [Suisse] ; Jérémy Bonvin [Suisse] ; Ian R. Sanders [Suisse]Source :
- Mycorrhiza [ 1432-1890 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Glomeromycota, Mycorhizes.
- microbiologie : Racines de plante.
- Techniques microbiologiques.
English descriptors
- KwdEn :
- MESH :
- growth & development : Glomeromycota, Mycorrhizae.
- microbiology : Plant Roots.
- Microbiological Techniques.
Abstract
One of the bottlenecks in mycorrhiza research is that arbuscular mycorrhizal fungi (AMF) have to be cultivated with host plant roots. Some AMF species, such as Rhizophagus irregularis, can be grown in vitro on dual-compartment plates, where fungal material can be harvested from a fungus-only compartment. Plant roots often grow into this fungus compartment, and regular root trimming is required if the fungal material needs to be free of traces of plant material. Trimming also increases unwanted contamination by other microorganisms. We compared 22 different culture types and conditions to a widely used dual-compartment culture system that we refer to as the "standard system." We found two modified culture systems that allowed high spore production and low rates of contamination. We then compared the two modified culture systems with the standard system in more detail. In the two modified culture systems versus the standard system, a comparable number of spores were produced per plate, the necessity for root trimming was reduced, and there was significantly diminished contamination in the fungal compartment. A cost analysis showed that both modified culture systems were more economic than the standard culture system for the production of the same number of non-contaminated spores. The two modified culture systems provide an economic alternative for the production of contaminant-free fungal material which is ideal for studies requiring AMF DNA or RNA for genetics, genomics, and transcriptomic studies or for studies requiring relatively large amounts of fungal material for greenhouse experiments.
DOI: 10.1007/s00572-017-0763-2
PubMed: 28210812
PubMed Central: PMC5486606
Links toward previous steps (curation, corpus...)
- to stream Main, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000D56
Links to Exploration step
pubmed:28210812Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cost-efficient production of in vitro Rhizophagus irregularis.</title><author><name sortKey="Rosikiewicz, Pawel" sort="Rosikiewicz, Pawel" uniqKey="Rosikiewicz P" first="Pawel" last="Rosikiewicz">Pawel Rosikiewicz</name><affiliation wicri:level="1"><nlm:affiliation>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne</wicri:regionArea></affiliation></author><author><name sortKey="Bonvin, Jeremy" sort="Bonvin, Jeremy" uniqKey="Bonvin J" first="Jérémy" last="Bonvin">Jérémy Bonvin</name><affiliation wicri:level="1"><nlm:affiliation>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne</wicri:regionArea></affiliation></author><author><name sortKey="Sanders, Ian R" sort="Sanders, Ian R" uniqKey="Sanders I" first="Ian R" last="Sanders">Ian R. Sanders</name><affiliation wicri:level="1"><nlm:affiliation>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne, Switzerland. ian.sanders@unil.ch.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28210812</idno><idno type="pmid">28210812</idno><idno type="doi">10.1007/s00572-017-0763-2</idno><idno type="pmc">PMC5486606</idno><idno type="wicri:Area/Main/Corpus">000D56</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000D56</idno><idno type="wicri:Area/Main/Curation">000D56</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000D56</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cost-efficient production of in vitro Rhizophagus irregularis.</title><author><name sortKey="Rosikiewicz, Pawel" sort="Rosikiewicz, Pawel" uniqKey="Rosikiewicz P" first="Pawel" last="Rosikiewicz">Pawel Rosikiewicz</name><affiliation wicri:level="1"><nlm:affiliation>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne</wicri:regionArea></affiliation></author><author><name sortKey="Bonvin, Jeremy" sort="Bonvin, Jeremy" uniqKey="Bonvin J" first="Jérémy" last="Bonvin">Jérémy Bonvin</name><affiliation wicri:level="1"><nlm:affiliation>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne</wicri:regionArea></affiliation></author><author><name sortKey="Sanders, Ian R" sort="Sanders, Ian R" uniqKey="Sanders I" first="Ian R" last="Sanders">Ian R. Sanders</name><affiliation wicri:level="1"><nlm:affiliation>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne, Switzerland. ian.sanders@unil.ch.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne</wicri:regionArea></affiliation></author></analytic><series><title level="j">Mycorrhiza</title><idno type="eISSN">1432-1890</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Glomeromycota (growth & development)</term><term>Microbiological Techniques (MeSH)</term><term>Mycorrhizae (growth & development)</term><term>Plant Roots (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Glomeromycota (croissance et développement)</term><term>Mycorhizes (croissance et développement)</term><term>Racines de plante (microbiologie)</term><term>Techniques microbiologiques (MeSH)</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Glomeromycota</term><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Glomeromycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Roots</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Microbiological Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Techniques microbiologiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">One of the bottlenecks in mycorrhiza research is that arbuscular mycorrhizal fungi (AMF) have to be cultivated with host plant roots. Some AMF species, such as Rhizophagus irregularis, can be grown in vitro on dual-compartment plates, where fungal material can be harvested from a fungus-only compartment. Plant roots often grow into this fungus compartment, and regular root trimming is required if the fungal material needs to be free of traces of plant material. Trimming also increases unwanted contamination by other microorganisms. We compared 22 different culture types and conditions to a widely used dual-compartment culture system that we refer to as the "standard system." We found two modified culture systems that allowed high spore production and low rates of contamination. We then compared the two modified culture systems with the standard system in more detail. In the two modified culture systems versus the standard system, a comparable number of spores were produced per plate, the necessity for root trimming was reduced, and there was significantly diminished contamination in the fungal compartment. A cost analysis showed that both modified culture systems were more economic than the standard culture system for the production of the same number of non-contaminated spores. The two modified culture systems provide an economic alternative for the production of contaminant-free fungal material which is ideal for studies requiring AMF DNA or RNA for genetics, genomics, and transcriptomic studies or for studies requiring relatively large amounts of fungal material for greenhouse experiments.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">28210812</PMID><DateCompleted><Year>2018</Year><Month>01</Month><Day>10</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-1890</ISSN><JournalIssue CitedMedium="Internet"><Volume>27</Volume><Issue>5</Issue><PubDate><Year>2017</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>Cost-efficient production of in vitro Rhizophagus irregularis.</ArticleTitle><Pagination><MedlinePgn>477-486</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00572-017-0763-2</ELocationID><Abstract><AbstractText>One of the bottlenecks in mycorrhiza research is that arbuscular mycorrhizal fungi (AMF) have to be cultivated with host plant roots. Some AMF species, such as Rhizophagus irregularis, can be grown in vitro on dual-compartment plates, where fungal material can be harvested from a fungus-only compartment. Plant roots often grow into this fungus compartment, and regular root trimming is required if the fungal material needs to be free of traces of plant material. Trimming also increases unwanted contamination by other microorganisms. We compared 22 different culture types and conditions to a widely used dual-compartment culture system that we refer to as the "standard system." We found two modified culture systems that allowed high spore production and low rates of contamination. We then compared the two modified culture systems with the standard system in more detail. In the two modified culture systems versus the standard system, a comparable number of spores were produced per plate, the necessity for root trimming was reduced, and there was significantly diminished contamination in the fungal compartment. A cost analysis showed that both modified culture systems were more economic than the standard culture system for the production of the same number of non-contaminated spores. The two modified culture systems provide an economic alternative for the production of contaminant-free fungal material which is ideal for studies requiring AMF DNA or RNA for genetics, genomics, and transcriptomic studies or for studies requiring relatively large amounts of fungal material for greenhouse experiments.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rosikiewicz</LastName><ForeName>Pawel</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bonvin</LastName><ForeName>Jérémy</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sanders</LastName><ForeName>Ian R</ForeName><Initials>IR</Initials><AffiliationInfo><Affiliation>Department of Ecology and Evolution, University of Lausanne, Biophore Building, 1015, Lausanne, Switzerland. ian.sanders@unil.ch.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>02</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Mycorrhiza</MedlineTA><NlmUniqueID>100955036</NlmUniqueID><ISSNLinking>0940-6360</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D055137" MajorTopicYN="N">Glomeromycota</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008828" MajorTopicYN="Y">Microbiological Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arbuscular mycorrhizal fungi</Keyword><Keyword MajorTopicYN="N">Inoculum production</Keyword><Keyword MajorTopicYN="N">Rhizophagus irregularis</Keyword><Keyword MajorTopicYN="N">Root-organ culture</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>10</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>01</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>2</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>1</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>2</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28210812</ArticleId><ArticleId IdType="doi">10.1007/s00572-017-0763-2</ArticleId><ArticleId IdType="pii">10.1007/s00572-017-0763-2</ArticleId><ArticleId IdType="pmc">PMC5486606</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Mycorrhiza. 2004 Aug;14(4):263-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12942358</ArticleId></ArticleIdList></Reference><Reference><Citation>ISME J. 2010 Sep;4(9):1081-3</Citation><ArticleIdList><ArticleId IdType="pubmed">20613789</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycorrhiza. 2002 Aug;12(4):163-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12189469</ArticleId></ArticleIdList></Reference><Reference><Citation>ISME J. 2016 Oct;10 (10 ):2514-26</Citation><ArticleIdList><ArticleId IdType="pubmed">26953600</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Aug 07;8(8):e70633</Citation><ArticleIdList><ArticleId IdType="pubmed">23950975</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2369-74</Citation><ArticleIdList><ArticleId IdType="pubmed">14983016</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycorrhiza. 2016 Apr;26(3):209-14</Citation><ArticleIdList><ArticleId IdType="pubmed">26403242</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MycorrhizaeV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D56 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 000D56 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MycorrhizaeV1
|flux= Main
|étape= Curation
|type= RBID
|clé= pubmed:28210812
|texte= Cost-efficient production of in vitro Rhizophagus irregularis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:28210812" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \
| NlmPubMed2Wicri -a MycorrhizaeV1
| This area was generated with Dilib version V0.6.37. |  |