Genetic transformation of the symbiotic basidiomycete fungus Hebeloma cylindrosporum.
Identifieur interne : 003B36 ( Main/Corpus ); précédent : 003B35; suivant : 003B37Genetic transformation of the symbiotic basidiomycete fungus Hebeloma cylindrosporum.
Auteurs : R. Marmeisse ; G. Gay ; J C Debaud ; L A CasseltonSource :
- Current genetics [ 0172-8083 ] ; 1992.
English descriptors
- KwdEn :
- Basidiomycota (cytology), Basidiomycota (metabolism), Blotting, Southern (MeSH), DNA, Fungal (MeSH), Drug Resistance, Microbial (MeSH), Glutamate Dehydrogenase (NADP+) (MeSH), Glutamate Dehydrogenase (genetics), Hygromycin B (MeSH), Mitosis (MeSH), Plasmids (MeSH), Symbiosis (MeSH), Transformation, Genetic (MeSH), Tryptophan (biosynthesis), Tryptophan (genetics).
- MESH :
- chemical , biosynthesis : Tryptophan.
- chemical , genetics : Glutamate Dehydrogenase, Tryptophan.
- chemical : DNA, Fungal, Glutamate Dehydrogenase (NADP+), Hygromycin B.
- cytology : Basidiomycota.
- metabolism : Basidiomycota.
- Blotting, Southern, Drug Resistance, Microbial, Mitosis, Plasmids, Symbiosis, Transformation, Genetic.
Abstract
The pAN7.1 plasmid containing the E. coli hygromycin B phosphotransferase gene was used to transform protoplasts of the ectomycorrhizal fungus Hebeloma cylindrosporum. Hygromycin-resistant transformants were selected at a frequency of one to five per micrograms of transforming DNA. Southern blot analyses revealed multiple copy integration of the transforming plasmid into the genome. The selection system was used to introduce other genes of interest by co-transformation. Two plasmids, one containing tryptophan biosynthesis genes and the other the NADP-glutamate dehydrogenase gene from the saprophytic basidiomycete Coprinus cinereus, were successfully introduced into the H. cylindrosporum genome with up to 70% efficiency of co-transformation. The hygromycin resistance phenotype was stably maintained during growth of transformants on hygromycin-free medium. All transformants retained their ability to form mycorrhizae with the habitual host plant Pinus pinaster, making them suitable for future physiological studies.
DOI: 10.1007/BF00351740
PubMed: 1319284
Links to Exploration step
pubmed:1319284Le document en format XML
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Gay</name></author><author><name sortKey="Debaud, J C" sort="Debaud, J C" uniqKey="Debaud J" first="J C" last="Debaud">J C Debaud</name></author><author><name sortKey="Casselton, L A" sort="Casselton, L A" uniqKey="Casselton L" first="L A" last="Casselton">L A Casselton</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1319284</idno><idno type="pmid">1319284</idno><idno type="doi">10.1007/BF00351740</idno><idno type="wicri:Area/Main/Corpus">003B36</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003B36</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genetic transformation of the symbiotic basidiomycete fungus Hebeloma cylindrosporum.</title><author><name sortKey="Marmeisse, R" sort="Marmeisse, R" uniqKey="Marmeisse R" first="R" last="Marmeisse">R. Marmeisse</name><affiliation><nlm:affiliation>School of Biological Sciences, Queen Mary and Westfield College, London, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Gay, G" sort="Gay, G" uniqKey="Gay G" first="G" last="Gay">G. Gay</name></author><author><name sortKey="Debaud, J C" sort="Debaud, J C" uniqKey="Debaud J" first="J C" last="Debaud">J C Debaud</name></author><author><name sortKey="Casselton, L A" sort="Casselton, L A" uniqKey="Casselton L" first="L A" last="Casselton">L A Casselton</name></author></analytic><series><title level="j">Current genetics</title><idno type="ISSN">0172-8083</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (cytology)</term><term>Basidiomycota (metabolism)</term><term>Blotting, Southern (MeSH)</term><term>DNA, Fungal (MeSH)</term><term>Drug Resistance, Microbial (MeSH)</term><term>Glutamate Dehydrogenase (NADP+) (MeSH)</term><term>Glutamate Dehydrogenase (genetics)</term><term>Hygromycin B (MeSH)</term><term>Mitosis (MeSH)</term><term>Plasmids (MeSH)</term><term>Symbiosis (MeSH)</term><term>Transformation, Genetic (MeSH)</term><term>Tryptophan (biosynthesis)</term><term>Tryptophan (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Tryptophan</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glutamate Dehydrogenase</term><term>Tryptophan</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Fungal</term><term>Glutamate Dehydrogenase (NADP+)</term><term>Hygromycin B</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Southern</term><term>Drug Resistance, Microbial</term><term>Mitosis</term><term>Plasmids</term><term>Symbiosis</term><term>Transformation, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pAN7.1 plasmid containing the E. coli hygromycin B phosphotransferase gene was used to transform protoplasts of the ectomycorrhizal fungus Hebeloma cylindrosporum. Hygromycin-resistant transformants were selected at a frequency of one to five per micrograms of transforming DNA. Southern blot analyses revealed multiple copy integration of the transforming plasmid into the genome. The selection system was used to introduce other genes of interest by co-transformation. Two plasmids, one containing tryptophan biosynthesis genes and the other the NADP-glutamate dehydrogenase gene from the saprophytic basidiomycete Coprinus cinereus, were successfully introduced into the H. cylindrosporum genome with up to 70% efficiency of co-transformation. The hygromycin resistance phenotype was stably maintained during growth of transformants on hygromycin-free medium. All transformants retained their ability to form mycorrhizae with the habitual host plant Pinus pinaster, making them suitable for future physiological studies.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1319284</PMID><DateCompleted><Year>1992</Year><Month>07</Month><Day>29</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0172-8083</ISSN><JournalIssue CitedMedium="Print"><Volume>22</Volume><Issue>1</Issue><PubDate><Year>1992</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Current genetics</Title><ISOAbbreviation>Curr Genet</ISOAbbreviation></Journal><ArticleTitle>Genetic transformation of the symbiotic basidiomycete fungus Hebeloma cylindrosporum.</ArticleTitle><Pagination><MedlinePgn>41-5</MedlinePgn></Pagination><Abstract><AbstractText>The pAN7.1 plasmid containing the E. coli hygromycin B phosphotransferase gene was used to transform protoplasts of the ectomycorrhizal fungus Hebeloma cylindrosporum. Hygromycin-resistant transformants were selected at a frequency of one to five per micrograms of transforming DNA. Southern blot analyses revealed multiple copy integration of the transforming plasmid into the genome. The selection system was used to introduce other genes of interest by co-transformation. Two plasmids, one containing tryptophan biosynthesis genes and the other the NADP-glutamate dehydrogenase gene from the saprophytic basidiomycete Coprinus cinereus, were successfully introduced into the H. cylindrosporum genome with up to 70% efficiency of co-transformation. The hygromycin resistance phenotype was stably maintained during growth of transformants on hygromycin-free medium. All transformants retained their ability to form mycorrhizae with the habitual host plant Pinus pinaster, making them suitable for future physiological studies.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Marmeisse</LastName><ForeName>R</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>School of Biological Sciences, Queen Mary and Westfield College, London, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gay</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Debaud</LastName><ForeName>J C</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Casselton</LastName><ForeName>L A</ForeName><Initials>LA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Curr Genet</MedlineTA><NlmUniqueID>8004904</NlmUniqueID><ISSNLinking>0172-8083</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>3XQ2233B0B</RegistryNumber><NameOfSubstance UI="D006921">Hygromycin B</NameOfSubstance></Chemical><Chemical><RegistryNumber>8DUH1N11BX</RegistryNumber><NameOfSubstance UI="D014364">Tryptophan</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.4.1.2</RegistryNumber><NameOfSubstance UI="D005969">Glutamate Dehydrogenase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.4.1.4</RegistryNumber><NameOfSubstance UI="D018086">Glutamate Dehydrogenase (NADP+)</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015139" MajorTopicYN="N">Blotting, Southern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004352" MajorTopicYN="N">Drug Resistance, Microbial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005969" MajorTopicYN="N">Glutamate Dehydrogenase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018086" MajorTopicYN="N">Glutamate Dehydrogenase (NADP+)</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006921" MajorTopicYN="N">Hygromycin B</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008938" MajorTopicYN="N">Mitosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014364" MajorTopicYN="N">Tryptophan</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year><Month>7</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1992</Year><Month>7</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1992</Year><Month>7</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1319284</ArticleId><ArticleId IdType="doi">10.1007/BF00351740</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Bacteriol. 1969 Dec;100(3):1222-8</Citation><ArticleIdList><ArticleId IdType="pubmed">5391229</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Gen Genet. 1990 Jun;222(1):41-8</Citation><ArticleIdList><ArticleId IdType="pubmed">1700269</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 1986;49(1):9-22</Citation><ArticleIdList><ArticleId IdType="pubmed">3552884</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Biol. 1986 Jan;6(1):195-200</Citation><ArticleIdList><ArticleId IdType="pubmed">3785146</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 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