Molecular tools for the identification of ectomycorrhizal fungi--taxon-specific oligonucleotide probes for suilloid fungi.
Identifieur interne : 003B31 ( Main/Corpus ); précédent : 003B30; suivant : 003B32Molecular tools for the identification of ectomycorrhizal fungi--taxon-specific oligonucleotide probes for suilloid fungi.
Auteurs : T D Bruns ; M. GardesSource :
- Molecular ecology [ 0962-1083 ] ; 1993.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Basidiomycota (classification), Basidiomycota (genetics), DNA Primers (MeSH), Molecular Sequence Data (MeSH), Oligonucleotide Probes (MeSH), Polymerase Chain Reaction (MeSH), RNA (genetics), RNA, Fungal (genetics), RNA, Mitochondrial (MeSH), RNA, Ribosomal (genetics), Sequence Homology, Nucleic Acid (MeSH), Species Specificity (MeSH).
- MESH :
- chemical , genetics : RNA, RNA, Fungal, RNA, Ribosomal.
- chemical : DNA Primers, Oligonucleotide Probes, RNA, Mitochondrial.
- classification : Basidiomycota.
- genetics : Basidiomycota.
- Base Sequence, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Homology, Nucleic Acid, Species Specificity.
Abstract
Five taxon-specific oligonucleotide probes are described that can be used to help identify the fungal components of ectomycorrhizae. Comparisons among partial sequence from the mitochondrial large subunit rRNA gene (mt-LrRNA) were used to select the probes, which were intended to be specific to several taxa within the suilloid group of the Boletales (Basidiomycota). Probes S1, R1, and G1 were targeted at the genera Suillus, Rhizopogon and Gomphidius; probe G2 was designed to recognize the family, Gomphidiaceae, and probe US1 was designed to recognize all of these taxa and any other members of the suilloid group. The specificity of each probe was determined empirically by testing their ability to hybridize to PCR amplified fragments derived from 84 species of basidiomycetes. Although none of the probes exhibited their intended specificity, all specifically hybridized to useful subsets of taxa, and collectively they can be used to identify many suilloid taxa to the generic level or below. The probes were also tested for their ability to identify field collected mycorrhizae and were found to perform well.
DOI: 10.1111/j.1365-294x.1993.tb00013.x
PubMed: 7513242
Links to Exploration step
pubmed:7513242Le document en format XML
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<author><name sortKey="Bruns, T D" sort="Bruns, T D" uniqKey="Bruns T" first="T D" last="Bruns">T D Bruns</name>
<affiliation><nlm:affiliation>Department of Plant Pathology, University of California, Berkeley 94720.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Gardes, M" sort="Gardes, M" uniqKey="Gardes M" first="M" last="Gardes">M. Gardes</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular tools for the identification of ectomycorrhizal fungi--taxon-specific oligonucleotide probes for suilloid fungi.</title>
<author><name sortKey="Bruns, T D" sort="Bruns, T D" uniqKey="Bruns T" first="T D" last="Bruns">T D Bruns</name>
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<series><title level="j">Molecular ecology</title>
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<imprint><date when="1993" type="published">1993</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term>
<term>Basidiomycota (classification)</term>
<term>Basidiomycota (genetics)</term>
<term>DNA Primers (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Oligonucleotide Probes (MeSH)</term>
<term>Polymerase Chain Reaction (MeSH)</term>
<term>RNA (genetics)</term>
<term>RNA, Fungal (genetics)</term>
<term>RNA, Mitochondrial (MeSH)</term>
<term>RNA, Ribosomal (genetics)</term>
<term>Sequence Homology, Nucleic Acid (MeSH)</term>
<term>Species Specificity (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA</term>
<term>RNA, Fungal</term>
<term>RNA, Ribosomal</term>
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<keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term>
<term>Oligonucleotide Probes</term>
<term>RNA, Mitochondrial</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Molecular Sequence Data</term>
<term>Polymerase Chain Reaction</term>
<term>Sequence Homology, Nucleic Acid</term>
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<front><div type="abstract" xml:lang="en">Five taxon-specific oligonucleotide probes are described that can be used to help identify the fungal components of ectomycorrhizae. Comparisons among partial sequence from the mitochondrial large subunit rRNA gene (mt-LrRNA) were used to select the probes, which were intended to be specific to several taxa within the suilloid group of the Boletales (Basidiomycota). Probes S1, R1, and G1 were targeted at the genera Suillus, Rhizopogon and Gomphidius; probe G2 was designed to recognize the family, Gomphidiaceae, and probe US1 was designed to recognize all of these taxa and any other members of the suilloid group. The specificity of each probe was determined empirically by testing their ability to hybridize to PCR amplified fragments derived from 84 species of basidiomycetes. Although none of the probes exhibited their intended specificity, all specifically hybridized to useful subsets of taxa, and collectively they can be used to identify many suilloid taxa to the generic level or below. The probes were also tested for their ability to identify field collected mycorrhizae and were found to perform well.</div>
</front>
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<Month>06</Month>
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<DateRevised><Year>2019</Year>
<Month>09</Month>
<Day>13</Day>
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<Article PubModel="Print"><Journal><ISSN IssnType="Print">0962-1083</ISSN>
<JournalIssue CitedMedium="Print"><Volume>2</Volume>
<Issue>4</Issue>
<PubDate><Year>1993</Year>
<Month>Aug</Month>
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<Title>Molecular ecology</Title>
<ISOAbbreviation>Mol Ecol</ISOAbbreviation>
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<ArticleTitle>Molecular tools for the identification of ectomycorrhizal fungi--taxon-specific oligonucleotide probes for suilloid fungi.</ArticleTitle>
<Pagination><MedlinePgn>233-42</MedlinePgn>
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<Abstract><AbstractText>Five taxon-specific oligonucleotide probes are described that can be used to help identify the fungal components of ectomycorrhizae. Comparisons among partial sequence from the mitochondrial large subunit rRNA gene (mt-LrRNA) were used to select the probes, which were intended to be specific to several taxa within the suilloid group of the Boletales (Basidiomycota). Probes S1, R1, and G1 were targeted at the genera Suillus, Rhizopogon and Gomphidius; probe G2 was designed to recognize the family, Gomphidiaceae, and probe US1 was designed to recognize all of these taxa and any other members of the suilloid group. The specificity of each probe was determined empirically by testing their ability to hybridize to PCR amplified fragments derived from 84 species of basidiomycetes. Although none of the probes exhibited their intended specificity, all specifically hybridized to useful subsets of taxa, and collectively they can be used to identify many suilloid taxa to the generic level or below. The probes were also tested for their ability to identify field collected mycorrhizae and were found to perform well.</AbstractText>
</Abstract>
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<Language>eng</Language>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
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<MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName>
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<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
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<MeshHeading><DescriptorName UI="D015345" MajorTopicYN="N">Oligonucleotide Probes</DescriptorName>
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<MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName>
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