Increased spore production by Glomus intraradices in the split-plate monoxenic culture system by repeated harvest, gel replacement, and resupply of glucose to the mycorrhiza.
Identifieur interne : 003A34 ( Main/Corpus ); précédent : 003A33; suivant : 003A35Increased spore production by Glomus intraradices in the split-plate monoxenic culture system by repeated harvest, gel replacement, and resupply of glucose to the mycorrhiza.
Auteurs : David D. DoudsSource :
- Mycorrhiza [ 0940-6360 ] ; 2002.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Glucose.
- chemical : Culture Media.
- growth & development : Fungi, Mycorrhizae, Spores, Fungal.
- microbiology : Daucus carota, Plant Roots.
Abstract
Monoxenic culture of Glomus intraradices Schenck and Smith with Ri T-DNA transformed roots in two-compartment Petri dishes is a very useful technique for physiological studies and the production of clean fungal tissues. Experiments were conducted to increase the efficiency of this method for the production of arbuscular mycorrhizal fungus spores. Approximately 20,000 spores could be harvested every 2 months from the distal (fungus only) compartment of a 9-cm-diameter divided Petri dish. The method requires replacement of the gelled media in the distal compartment and resupply of 200 mg glucose to the proximal (root) compartment coincident with harvest of spores. These modifications resulted in an approximate threefold increase in spore production per unit time over the standard split-plate culture technique.
DOI: 10.1007/s00572-002-0174-9
PubMed: 12189469
Links to Exploration step
pubmed:12189469Le document en format XML
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Mermaid Lane, Wyndmoor, PA 19038, USA. ddouds@arserrc.gov</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:12189469</idno><idno type="pmid">12189469</idno><idno type="doi">10.1007/s00572-002-0174-9</idno><idno type="wicri:Area/Main/Corpus">003A34</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003A34</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Increased spore production by Glomus intraradices in the split-plate monoxenic culture system by repeated harvest, gel replacement, and resupply of glucose to the mycorrhiza.</title><author><name sortKey="Douds, David D" sort="Douds, David D" uniqKey="Douds D" first="David D" last="Douds">David D. Douds</name><affiliation><nlm:affiliation>USDA-ARS ERRC, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA. ddouds@arserrc.gov</nlm:affiliation></affiliation></author></analytic><series><title level="j">Mycorrhiza</title><idno type="ISSN">0940-6360</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Culture Media (MeSH)</term><term>Daucus carota (microbiology)</term><term>Fungi (growth & development)</term><term>Glucose (metabolism)</term><term>Mycorrhizae (growth & development)</term><term>Plant Roots (microbiology)</term><term>Spores, Fungal (growth & development)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucose</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Fungi</term><term>Mycorrhizae</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Daucus carota</term><term>Plant Roots</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Monoxenic culture of Glomus intraradices Schenck and Smith with Ri T-DNA transformed roots in two-compartment Petri dishes is a very useful technique for physiological studies and the production of clean fungal tissues. Experiments were conducted to increase the efficiency of this method for the production of arbuscular mycorrhizal fungus spores. Approximately 20,000 spores could be harvested every 2 months from the distal (fungus only) compartment of a 9-cm-diameter divided Petri dish. The method requires replacement of the gelled media in the distal compartment and resupply of 200 mg glucose to the proximal (root) compartment coincident with harvest of spores. These modifications resulted in an approximate threefold increase in spore production per unit time over the standard split-plate culture technique.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12189469</PMID><DateCompleted><Year>2003</Year><Month>02</Month><Day>06</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0940-6360</ISSN><JournalIssue CitedMedium="Print"><Volume>12</Volume><Issue>4</Issue><PubDate><Year>2002</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>Increased spore production by Glomus intraradices in the split-plate monoxenic culture system by repeated harvest, gel replacement, and resupply of glucose to the mycorrhiza.</ArticleTitle><Pagination><MedlinePgn>163-7</MedlinePgn></Pagination><Abstract><AbstractText>Monoxenic culture of Glomus intraradices Schenck and Smith with Ri T-DNA transformed roots in two-compartment Petri dishes is a very useful technique for physiological studies and the production of clean fungal tissues. Experiments were conducted to increase the efficiency of this method for the production of arbuscular mycorrhizal fungus spores. Approximately 20,000 spores could be harvested every 2 months from the distal (fungus only) compartment of a 9-cm-diameter divided Petri dish. The method requires replacement of the gelled media in the distal compartment and resupply of 200 mg glucose to the proximal (root) compartment coincident with harvest of spores. These modifications resulted in an approximate threefold increase in spore production per unit time over the standard split-plate culture technique.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Douds</LastName><ForeName>David D</ForeName><Initials>DD</Initials><Suffix>Jr</Suffix><AffiliationInfo><Affiliation>USDA-ARS ERRC, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA. ddouds@arserrc.gov</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2002</Year><Month>05</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Mycorrhiza</MedlineTA><NlmUniqueID>100955036</NlmUniqueID><ISSNLinking>0940-6360</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>IY9XDZ35W2</RegistryNumber><NameOfSubstance UI="D005947">Glucose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018552" MajorTopicYN="N">Daucus carota</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013172" MajorTopicYN="N">Spores, Fungal</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2001</Year><Month>11</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2002</Year><Month>03</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>8</Month><Day>22</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>2</Month><Day>7</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>8</Month><Day>22</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12189469</ArticleId><ArticleId IdType="doi">10.1007/s00572-002-0174-9</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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