A technical trick for studying proteomics in parallel to transcriptomics in symbiotic root-fungus interactions.
Identifieur interne : 003792 ( Main/Corpus ); précédent : 003791; suivant : 003793A technical trick for studying proteomics in parallel to transcriptomics in symbiotic root-fungus interactions.
Auteurs : Eliane Dumas-Gaudot ; Nardjis Amiour ; Stéphanie Weidmann ; Gwénaëlle Bestel-Corre ; Benoit Valot ; Sébastien Lenogue ; Vivienne Gianinazzi-Pearson ; Silvio GianinazziSource :
- Proteomics [ 1615-9853 ] ; 2004.
English descriptors
- KwdEn :
- Electrophoresis, Gel, Two-Dimensional (MeSH), Fungal Proteins (analysis), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Medicago (cytology), Medicago (genetics), Medicago (metabolism), Mycorrhizae (cytology), Mycorrhizae (genetics), Mycorrhizae (metabolism), Plant Roots (metabolism), Proteome (analysis), RNA, Messenger (analysis), Symbiosis (physiology).
- MESH :
- chemical , analysis : Fungal Proteins, Proteome, RNA, Messenger.
- cytology : Medicago, Mycorrhizae.
- genetics : Medicago, Mycorrhizae.
- metabolism : Medicago, Mycorrhizae, Plant Roots.
- physiology : Symbiosis.
- Electrophoresis, Gel, Two-Dimensional, Gene Expression Profiling, Gene Expression Regulation, Plant.
Abstract
We have developed a protocol in which proteins and mRNA can be analyzed from single root samples. This experimental design was validated in arbuscular mycorrhiza by comparing the proteins profiles obtained with those from a classical protein extraction process. It is a step forward to make simultaneous proteome and transcriptiome profiling possible.
DOI: 10.1002/pmic.200300627
PubMed: 14760717
Links to Exploration step
pubmed:14760717Le document en format XML
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Bestel-Corre</name></author><author><name sortKey="Valot, Benoit" sort="Valot, Benoit" uniqKey="Valot B" first="Benoit" last="Valot">Benoit Valot</name></author><author><name sortKey="Lenogue, Sebastien" sort="Lenogue, Sebastien" uniqKey="Lenogue S" first="Sébastien" last="Lenogue">Sébastien Lenogue</name></author><author><name sortKey="Gianinazzi Pearson, Vivienne" sort="Gianinazzi Pearson, Vivienne" uniqKey="Gianinazzi Pearson V" first="Vivienne" last="Gianinazzi-Pearson">Vivienne Gianinazzi-Pearson</name></author><author><name sortKey="Gianinazzi, Silvio" sort="Gianinazzi, Silvio" uniqKey="Gianinazzi S" first="Silvio" last="Gianinazzi">Silvio Gianinazzi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:14760717</idno><idno type="pmid">14760717</idno><idno type="doi">10.1002/pmic.200300627</idno><idno type="wicri:Area/Main/Corpus">003792</idno><idno type="wicri:explorRef" wicri:stream="Main" 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xml:lang="en"><term>Electrophoresis, Gel, Two-Dimensional (MeSH)</term><term>Fungal Proteins (analysis)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Medicago (cytology)</term><term>Medicago (genetics)</term><term>Medicago (metabolism)</term><term>Mycorrhizae (cytology)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (metabolism)</term><term>Plant Roots (metabolism)</term><term>Proteome (analysis)</term><term>RNA, Messenger (analysis)</term><term>Symbiosis (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Fungal Proteins</term><term>Proteome</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Medicago</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Medicago</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Medicago</term><term>Mycorrhizae</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electrophoresis, Gel, Two-Dimensional</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have developed a protocol in which proteins and mRNA can be analyzed from single root samples. This experimental design was validated in arbuscular mycorrhiza by comparing the proteins profiles obtained with those from a classical protein extraction process. It is a step forward to make simultaneous proteome and transcriptiome profiling possible.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14760717</PMID><DateCompleted><Year>2004</Year><Month>09</Month><Day>21</Day></DateCompleted><DateRevised><Year>2004</Year><Month>02</Month><Day>04</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1615-9853</ISSN><JournalIssue CitedMedium="Print"><Volume>4</Volume><Issue>2</Issue><PubDate><Year>2004</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Proteomics</Title><ISOAbbreviation>Proteomics</ISOAbbreviation></Journal><ArticleTitle>A technical trick for studying proteomics in parallel to transcriptomics in symbiotic root-fungus interactions.</ArticleTitle><Pagination><MedlinePgn>451-3</MedlinePgn></Pagination><Abstract><AbstractText>We have developed a protocol in which proteins and mRNA can be analyzed from single root samples. This experimental design was validated in arbuscular mycorrhiza by comparing the proteins profiles obtained with those from a classical protein extraction process. It is a step forward to make simultaneous proteome and transcriptiome profiling possible.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dumas-Gaudot</LastName><ForeName>Eliane</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>UMR 1088 INRA/CNRS 5184/U, Bourgogne (Plante-Microbe- Environnement) INRA-CMSE, Dijon, France. dumas@epoisses.inra.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Amiour</LastName><ForeName>Nardjis</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Weidmann</LastName><ForeName>Stéphanie</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Bestel-Corre</LastName><ForeName>Gwénaëlle</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Valot</LastName><ForeName>Benoit</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Lenogue</LastName><ForeName>Sébastien</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Gianinazzi-Pearson</LastName><ForeName>Vivienne</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Gianinazzi</LastName><ForeName>Silvio</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Proteomics</MedlineTA><NlmUniqueID>101092707</NlmUniqueID><ISSNLinking>1615-9853</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029902" MajorTopicYN="N">Medicago</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>2</Month><Day>5</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>9</Month><Day>24</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>2</Month><Day>5</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14760717</ArticleId><ArticleId IdType="doi">10.1002/pmic.200300627</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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