Vertical transmission of endobacteria in the arbuscular mycorrhizal fungus Gigaspora margarita through generation of vegetative spores.
Identifieur interne : 003737 ( Main/Corpus ); précédent : 003736; suivant : 003738Vertical transmission of endobacteria in the arbuscular mycorrhizal fungus Gigaspora margarita through generation of vegetative spores.
Auteurs : V. Bianciotto ; A. Genre ; P. Jargeat ; E. Lumini ; G. Bécard ; P. BonfanteSource :
- Applied and environmental microbiology [ 0099-2240 ] ; 2004.
English descriptors
- KwdEn :
- Bacteria (classification), Bacteria (genetics), Bacteria (growth & development), Bacteria (isolation & purification), Culture Media (MeSH), Cytoplasm (microbiology), DNA, Ribosomal (analysis), Daucus carota (microbiology), Fungi (genetics), Fungi (physiology), Fungi (ultrastructure), Gene Transfer, Horizontal (MeSH), Microscopy, Confocal (MeSH), Molecular Sequence Data (MeSH), Mycorrhizae (MeSH), Plant Roots (microbiology), Polymerase Chain Reaction (MeSH), RNA, Ribosomal, 23S (genetics), Soil Microbiology (MeSH), Spores, Fungal (genetics), Symbiosis (MeSH).
- MESH :
- chemical , analysis : DNA, Ribosomal.
- chemical , genetics : RNA, Ribosomal, 23S.
- chemical : Culture Media.
- classification : Bacteria.
- genetics : Bacteria, Fungi, Spores, Fungal.
- growth & development : Bacteria.
- isolation & purification : Bacteria.
- microbiology : Cytoplasm, Daucus carota, Plant Roots.
- physiology : Fungi.
- ultrastructure : Fungi.
- Gene Transfer, Horizontal, Microscopy, Confocal, Molecular Sequence Data, Mycorrhizae, Polymerase Chain Reaction, Soil Microbiology, Symbiosis.
Abstract
Arbuscular mycorrhizal (AM) fungi living in symbiotic association with the roots of vascular plants have also been shown to host endocellular rod-shaped bacteria. Based on their ribosomal sequences, these endobacteria have recently been identified as a new taxon, Candidatus Glomeribacter gigasporarum. In order to investigate the cytoplasmic stability of the endobacteria in their fungal host and their transmission during AM fungal reproduction (asexual), a system based on transformed carrot roots and single-spore inocula of Gigaspora margarita was used. Under these in vitro sterile conditions, with no risk of horizontal contamination, the propagation of endobacteria could be monitored, and it was shown, by using primers designed for both 16S and 23S ribosomal DNAs, to occur through several vegetative spore generations (SG0 to SG4). A method of confocal microscopy for quantifying the density of endobacteria in spore cytoplasm was designed and applied; endobacteria were consistently found in all of the spore generations, although their number rapidly decreased from SG0 to SG4. The study demonstrates that a vertical transmission of endobacteria takes place through the fungal vegetative generations (sporulation) of an AM fungus, indicating that active bacterial proliferation occurs in the coenocytic mycelium of the fungus, and suggests that these bacteria are obligate endocellular components of their AM fungal host.
DOI: 10.1128/AEM.70.6.3600-3608.2004
PubMed: 15184163
PubMed Central: PMC427789
Links to Exploration step
pubmed:15184163Le document en format XML
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Bécard</name></author><author><name sortKey="Bonfante, P" sort="Bonfante, P" uniqKey="Bonfante P" first="P" last="Bonfante">P. Bonfante</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15184163</idno><idno type="pmid">15184163</idno><idno type="doi">10.1128/AEM.70.6.3600-3608.2004</idno><idno type="pmc">PMC427789</idno><idno type="wicri:Area/Main/Corpus">003737</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003737</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Vertical transmission of endobacteria in the arbuscular mycorrhizal fungus Gigaspora margarita through generation of vegetative spores.</title><author><name sortKey="Bianciotto, V" sort="Bianciotto, V" uniqKey="Bianciotto V" first="V" last="Bianciotto">V. 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Based on their ribosomal sequences, these endobacteria have recently been identified as a new taxon, Candidatus Glomeribacter gigasporarum. In order to investigate the cytoplasmic stability of the endobacteria in their fungal host and their transmission during AM fungal reproduction (asexual), a system based on transformed carrot roots and single-spore inocula of Gigaspora margarita was used. Under these in vitro sterile conditions, with no risk of horizontal contamination, the propagation of endobacteria could be monitored, and it was shown, by using primers designed for both 16S and 23S ribosomal DNAs, to occur through several vegetative spore generations (SG0 to SG4). A method of confocal microscopy for quantifying the density of endobacteria in spore cytoplasm was designed and applied; endobacteria were consistently found in all of the spore generations, although their number rapidly decreased from SG0 to SG4. The study demonstrates that a vertical transmission of endobacteria takes place through the fungal vegetative generations (sporulation) of an AM fungus, indicating that active bacterial proliferation occurs in the coenocytic mycelium of the fungus, and suggests that these bacteria are obligate endocellular components of their AM fungal host.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15184163</PMID><DateCompleted><Year>2004</Year><Month>08</Month><Day>10</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0099-2240</ISSN><JournalIssue CitedMedium="Print"><Volume>70</Volume><Issue>6</Issue><PubDate><Year>2004</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Vertical transmission of endobacteria in the arbuscular mycorrhizal fungus Gigaspora margarita through generation of vegetative spores.</ArticleTitle><Pagination><MedlinePgn>3600-8</MedlinePgn></Pagination><Abstract><AbstractText>Arbuscular mycorrhizal (AM) fungi living in symbiotic association with the roots of vascular plants have also been shown to host endocellular rod-shaped bacteria. Based on their ribosomal sequences, these endobacteria have recently been identified as a new taxon, Candidatus Glomeribacter gigasporarum. In order to investigate the cytoplasmic stability of the endobacteria in their fungal host and their transmission during AM fungal reproduction (asexual), a system based on transformed carrot roots and single-spore inocula of Gigaspora margarita was used. Under these in vitro sterile conditions, with no risk of horizontal contamination, the propagation of endobacteria could be monitored, and it was shown, by using primers designed for both 16S and 23S ribosomal DNAs, to occur through several vegetative spore generations (SG0 to SG4). A method of confocal microscopy for quantifying the density of endobacteria in spore cytoplasm was designed and applied; endobacteria were consistently found in all of the spore generations, although their number rapidly decreased from SG0 to SG4. The study demonstrates that a vertical transmission of endobacteria takes place through the fungal vegetative generations (sporulation) of an AM fungus, indicating that active bacterial proliferation occurs in the coenocytic mycelium of the fungus, and suggests that these bacteria are obligate endocellular components of their AM fungal host.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bianciotto</LastName><ForeName>V</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Dipartimento di Biologia Vegetale dell'Università e Istituto per la Protezione delle Piante CNR, 10125 Turin, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Genre</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Jargeat</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Lumini</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author 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