Divergence in gene expression related to variation in host specificity of an ectomycorrhizal fungus.
Identifieur interne : 003658 ( Main/Corpus ); précédent : 003657; suivant : 003659Divergence in gene expression related to variation in host specificity of an ectomycorrhizal fungus.
Auteurs : Antoine Le Quéré ; Andres Schützendübel ; Balaji Rajashekar ; Björn Canb Ck ; Jenny Hedh ; Susanne Erland ; Tomas Johansson ; Anders TunlidSource :
- Molecular ecology [ 0962-1083 ] ; 2004.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Basidiomycota (genetics), Basidiomycota (metabolism), DNA Primers (MeSH), DNA, Ribosomal Spacer (genetics), Evolution, Molecular (MeSH), Gene Expression Regulation, Fungal (genetics), Likelihood Functions (MeSH), Models, Genetic (MeSH), Molecular Sequence Data (MeSH), Mycorrhizae (genetics), Mycorrhizae (metabolism), Oligonucleotide Array Sequence Analysis (MeSH), Phylogeny (MeSH), Sequence Analysis, DNA (MeSH), Species Specificity (MeSH), Symbiosis (MeSH).
- MESH :
- chemical , genetics : DNA, Ribosomal Spacer.
- chemical : DNA Primers.
- genetics : Basidiomycota, Gene Expression Regulation, Fungal, Mycorrhizae.
- metabolism : Basidiomycota, Mycorrhizae.
- Base Sequence, Evolution, Molecular, Likelihood Functions, Models, Genetic, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Phylogeny, Sequence Analysis, DNA, Species Specificity, Symbiosis.
Abstract
Ectomycorrhizae are formed by mutualistic interactions between fungi and the roots of woody plants. During symbiosis the two organisms exchange carbon and nutrients in a specific tissue that is formed at the contact between a compatible fungus and plant. There is considerable variation in the degree of host specificity among species and strains of ectomycorrhizal fungi. In this study, we have for the first time shown that this variation is associated with quantitative differences in gene expression, and with divergence in nucleotide sequences of symbiosis-regulated genes. Gene expression and sequence evolution were compared in different strains of the ectomycorrhizal fungus Paxillus involutus; the strains included Nau, which is not compatible with birch and poplar, and the two compatible strains Maj and ATCC200175. On a genomic level, Nau and Maj were very similar. The sequence identity was 98.9% in the 16 loci analysed, and only three out of 1075 genes analysed by microarray-based hybridizations had signals indicating differences in gene copy numbers. In contrast, 66 out of the 1075 genes were differentially expressed in Maj compared to Nau after contact with birch roots. Thirty-seven of these symbiosis-regulated genes were also differentially expressed in the ATCC strain. Comparative analysis of DNA sequences of the symbiosis-regulated genes in different strains showed that two of them have evolved at an enhanced rate in Nau. The sequence divergence can be explained by a decreased selection pressure, which in turn is determined by lower functional constraints on these proteins in Nau as compared to the compatible strains.
DOI: 10.1111/j.1365-294X.2004.02369.x
PubMed: 15548293
Links to Exploration step
pubmed:15548293Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Divergence in gene expression related to variation in host specificity of an ectomycorrhizal fungus.</title><author><name sortKey="Le Quere, Antoine" sort="Le Quere, Antoine" uniqKey="Le Quere A" first="Antoine" last="Le Quéré">Antoine Le Quéré</name><affiliation><nlm:affiliation>Department of Microbial Ecology, Lund University, Ecology Building, SE-223 62 Lund, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Schutzendubel, Andres" sort="Schutzendubel, Andres" uniqKey="Schutzendubel A" first="Andres" last="Schützendübel">Andres Schützendübel</name></author><author><name sortKey="Rajashekar, Balaji" sort="Rajashekar, Balaji" uniqKey="Rajashekar B" first="Balaji" last="Rajashekar">Balaji Rajashekar</name></author><author><name sortKey="Canb Ck, Bjorn" sort="Canb Ck, Bjorn" uniqKey="Canb Ck B" first="Björn" last="Canb Ck">Björn Canb Ck</name></author><author><name sortKey="Hedh, Jenny" sort="Hedh, Jenny" uniqKey="Hedh J" first="Jenny" last="Hedh">Jenny Hedh</name></author><author><name sortKey="Erland, Susanne" sort="Erland, Susanne" uniqKey="Erland S" first="Susanne" last="Erland">Susanne Erland</name></author><author><name sortKey="Johansson, Tomas" sort="Johansson, Tomas" uniqKey="Johansson T" first="Tomas" last="Johansson">Tomas Johansson</name></author><author><name sortKey="Tunlid, Anders" sort="Tunlid, Anders" uniqKey="Tunlid A" first="Anders" last="Tunlid">Anders Tunlid</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15548293</idno><idno type="pmid">15548293</idno><idno type="doi">10.1111/j.1365-294X.2004.02369.x</idno><idno type="wicri:Area/Main/Corpus">003658</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003658</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Divergence in gene expression related to variation in host specificity of an ectomycorrhizal fungus.</title><author><name sortKey="Le Quere, Antoine" sort="Le Quere, Antoine" uniqKey="Le Quere A" first="Antoine" last="Le Quéré">Antoine Le Quéré</name><affiliation><nlm:affiliation>Department of Microbial Ecology, Lund University, Ecology Building, SE-223 62 Lund, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Schutzendubel, Andres" sort="Schutzendubel, Andres" uniqKey="Schutzendubel A" first="Andres" last="Schützendübel">Andres Schützendübel</name></author><author><name sortKey="Rajashekar, Balaji" sort="Rajashekar, Balaji" uniqKey="Rajashekar B" first="Balaji" last="Rajashekar">Balaji Rajashekar</name></author><author><name sortKey="Canb Ck, Bjorn" sort="Canb Ck, Bjorn" uniqKey="Canb Ck B" first="Björn" last="Canb Ck">Björn Canb Ck</name></author><author><name sortKey="Hedh, Jenny" sort="Hedh, Jenny" uniqKey="Hedh J" first="Jenny" last="Hedh">Jenny Hedh</name></author><author><name sortKey="Erland, Susanne" sort="Erland, Susanne" uniqKey="Erland S" first="Susanne" last="Erland">Susanne Erland</name></author><author><name sortKey="Johansson, Tomas" sort="Johansson, Tomas" uniqKey="Johansson T" first="Tomas" last="Johansson">Tomas Johansson</name></author><author><name sortKey="Tunlid, Anders" sort="Tunlid, Anders" uniqKey="Tunlid A" first="Anders" last="Tunlid">Anders Tunlid</name></author></analytic><series><title level="j">Molecular ecology</title><idno type="ISSN">0962-1083</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Basidiomycota (genetics)</term><term>Basidiomycota (metabolism)</term><term>DNA Primers (MeSH)</term><term>DNA, Ribosomal Spacer (genetics)</term><term>Evolution, Molecular (MeSH)</term><term>Gene Expression Regulation, Fungal (genetics)</term><term>Likelihood Functions (MeSH)</term><term>Models, Genetic (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (metabolism)</term><term>Oligonucleotide Array Sequence Analysis (MeSH)</term><term>Phylogeny (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Species Specificity (MeSH)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Ribosomal Spacer</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term><term>Gene Expression Regulation, Fungal</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Evolution, Molecular</term><term>Likelihood Functions</term><term>Models, Genetic</term><term>Molecular Sequence Data</term><term>Oligonucleotide Array Sequence Analysis</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Species Specificity</term><term>Symbiosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ectomycorrhizae are formed by mutualistic interactions between fungi and the roots of woody plants. During symbiosis the two organisms exchange carbon and nutrients in a specific tissue that is formed at the contact between a compatible fungus and plant. There is considerable variation in the degree of host specificity among species and strains of ectomycorrhizal fungi. In this study, we have for the first time shown that this variation is associated with quantitative differences in gene expression, and with divergence in nucleotide sequences of symbiosis-regulated genes. Gene expression and sequence evolution were compared in different strains of the ectomycorrhizal fungus Paxillus involutus; the strains included Nau, which is not compatible with birch and poplar, and the two compatible strains Maj and ATCC200175. On a genomic level, Nau and Maj were very similar. The sequence identity was 98.9% in the 16 loci analysed, and only three out of 1075 genes analysed by microarray-based hybridizations had signals indicating differences in gene copy numbers. In contrast, 66 out of the 1075 genes were differentially expressed in Maj compared to Nau after contact with birch roots. Thirty-seven of these symbiosis-regulated genes were also differentially expressed in the ATCC strain. Comparative analysis of DNA sequences of the symbiosis-regulated genes in different strains showed that two of them have evolved at an enhanced rate in Nau. The sequence divergence can be explained by a decreased selection pressure, which in turn is determined by lower functional constraints on these proteins in Nau as compared to the compatible strains.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15548293</PMID><DateCompleted><Year>2005</Year><Month>02</Month><Day>04</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0962-1083</ISSN><JournalIssue CitedMedium="Print"><Volume>13</Volume><Issue>12</Issue><PubDate><Year>2004</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Molecular ecology</Title><ISOAbbreviation>Mol Ecol</ISOAbbreviation></Journal><ArticleTitle>Divergence in gene expression related to variation in host specificity of an ectomycorrhizal fungus.</ArticleTitle><Pagination><MedlinePgn>3809-19</MedlinePgn></Pagination><Abstract><AbstractText>Ectomycorrhizae are formed by mutualistic interactions between fungi and the roots of woody plants. During symbiosis the two organisms exchange carbon and nutrients in a specific tissue that is formed at the contact between a compatible fungus and plant. There is considerable variation in the degree of host specificity among species and strains of ectomycorrhizal fungi. In this study, we have for the first time shown that this variation is associated with quantitative differences in gene expression, and with divergence in nucleotide sequences of symbiosis-regulated genes. Gene expression and sequence evolution were compared in different strains of the ectomycorrhizal fungus Paxillus involutus; the strains included Nau, which is not compatible with birch and poplar, and the two compatible strains Maj and ATCC200175. On a genomic level, Nau and Maj were very similar. The sequence identity was 98.9% in the 16 loci analysed, and only three out of 1075 genes analysed by microarray-based hybridizations had signals indicating differences in gene copy numbers. In contrast, 66 out of the 1075 genes were differentially expressed in Maj compared to Nau after contact with birch roots. Thirty-seven of these symbiosis-regulated genes were also differentially expressed in the ATCC strain. Comparative analysis of DNA sequences of the symbiosis-regulated genes in different strains showed that two of them have evolved at an enhanced rate in Nau. The sequence divergence can be explained by a decreased selection pressure, which in turn is determined by lower functional constraints on these proteins in Nau as compared to the compatible strains.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Le Quéré</LastName><ForeName>Antoine</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Microbial Ecology, Lund University, Ecology Building, SE-223 62 Lund, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schützendübel</LastName><ForeName>Andres</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Rajashekar</LastName><ForeName>Balaji</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Canbäck</LastName><ForeName>Björn</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Hedh</LastName><ForeName>Jenny</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Erland</LastName><ForeName>Susanne</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Johansson</LastName><ForeName>Tomas</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Tunlid</LastName><ForeName>Anders</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Ecol</MedlineTA><NlmUniqueID>9214478</NlmUniqueID><ISSNLinking>0962-1083</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021903">DNA, Ribosomal Spacer</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021903" MajorTopicYN="N">DNA, Ribosomal Spacer</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019143" MajorTopicYN="Y">Evolution, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016013" MajorTopicYN="N">Likelihood Functions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008957" MajorTopicYN="N">Models, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020411" MajorTopicYN="N">Oligonucleotide Array Sequence Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="Y">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="Y">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>11</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>2</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>11</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15548293</ArticleId><ArticleId IdType="pii">MEC2369</ArticleId><ArticleId IdType="doi">10.1111/j.1365-294X.2004.02369.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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