Extraction of extraradical arbuscular mycorrhizal mycelium from compartments filled with soil and glass beads.
Identifieur interne : 003579 ( Main/Corpus ); précédent : 003578; suivant : 003580Extraction of extraradical arbuscular mycorrhizal mycelium from compartments filled with soil and glass beads.
Auteurs : Elke Neumann ; Eckhard GeorgeSource :
- Mycorrhiza [ 0940-6360 ] ; 2005.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Copper, Manganese, Phosphorus, Zinc.
- chemistry : Mycelium.
- isolation & purification : Mycelium, Mycorrhizae.
- microbiology : Plant Roots.
- Glass, Soil Microbiology.
Abstract
This study presents a novel method for the extraction and quantification of extraradical mycelium (ERM) of arbuscular mycorrhizal fungi (AMF) from a substrate that simulates soil better than previously used artificial growth media. Fungal compartments were constructed from small net pots with a latticed wall and filled with a mixture of glass beads and 40 microm wet sieved soil. The net pots were surrounded by a 30-microm mesh membrane through which hyphae but not roots could grow. They were inserted into soil where a Glomus intraradices (BEG 110) colonized potato plant was growing. The ERM that had grown out from roots through the membrane was successfully collected and quantified after harvest by washing out the soil/glass bead mixture through a sieve with a mesh width of 40 microm. Concentrations of P, Zn, Cu and Mn in the AMF ERM were analysed.
DOI: 10.1007/s00572-005-0361-6
PubMed: 15815921
Links to Exploration step
pubmed:15815921Le document en format XML
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Fungal compartments were constructed from small net pots with a latticed wall and filled with a mixture of glass beads and 40 microm wet sieved soil. The net pots were surrounded by a 30-microm mesh membrane through which hyphae but not roots could grow. They were inserted into soil where a Glomus intraradices (BEG 110) colonized potato plant was growing. The ERM that had grown out from roots through the membrane was successfully collected and quantified after harvest by washing out the soil/glass bead mixture through a sieve with a mesh width of 40 microm. Concentrations of P, Zn, Cu and Mn in the AMF ERM were analysed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15815921</PMID><DateCompleted><Year>2007</Year><Month>04</Month><Day>12</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0940-6360</ISSN><JournalIssue CitedMedium="Print"><Volume>15</Volume><Issue>7</Issue><PubDate><Year>2005</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>Extraction of extraradical arbuscular mycorrhizal mycelium from compartments filled with soil and glass beads.</ArticleTitle><Pagination><MedlinePgn>533-7</MedlinePgn></Pagination><Abstract><AbstractText>This study presents a novel method for the extraction and quantification of extraradical mycelium (ERM) of arbuscular mycorrhizal fungi (AMF) from a substrate that simulates soil better than previously used artificial growth media. Fungal compartments were constructed from small net pots with a latticed wall and filled with a mixture of glass beads and 40 microm wet sieved soil. The net pots were surrounded by a 30-microm mesh membrane through which hyphae but not roots could grow. They were inserted into soil where a Glomus intraradices (BEG 110) colonized potato plant was growing. The ERM that had grown out from roots through the membrane was successfully collected and quantified after harvest by washing out the soil/glass bead mixture through a sieve with a mesh width of 40 microm. 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