Nitrogen transfer in the arbuscular mycorrhizal symbiosis.
Identifieur interne : 003531 ( Main/Corpus ); précédent : 003530; suivant : 003532Nitrogen transfer in the arbuscular mycorrhizal symbiosis.
Auteurs : Manjula Govindarajulu ; Philip E. Pfeffer ; Hairu Jin ; Jehad Abubaker ; David D. Douds ; James W. Allen ; Heike Bücking ; Peter J. Lammers ; Yair Shachar-HillSource :
- Nature [ 1476-4687 ] ; 2005.
English descriptors
- KwdEn :
- Acetates (metabolism), Amino Acids (MeSH), Arginine (metabolism), DNA, Bacterial (genetics), Daucus carota (genetics), Daucus carota (metabolism), Daucus carota (microbiology), Gene Expression Regulation (MeSH), Genes, Fungal (genetics), Genes, Plant (genetics), Molecular Sequence Data (MeSH), Mycelium (metabolism), Mycorrhizae (genetics), Mycorrhizae (metabolism), Nitrates (metabolism), Nitrogen (metabolism), Symbiosis (genetics).
- MESH :
- chemical , genetics : DNA, Bacterial.
- chemical , metabolism : Acetates, Arginine, Nitrates, Nitrogen.
- chemical : Amino Acids.
- genetics : Daucus carota, Genes, Fungal, Genes, Plant, Mycorrhizae, Symbiosis.
- metabolism : Daucus carota, Mycelium, Mycorrhizae.
- microbiology : Daucus carota.
- Gene Expression Regulation, Molecular Sequence Data.
Abstract
Most land plants are symbiotic with arbuscular mycorrhizal fungi (AMF), which take up mineral nutrients from the soil and exchange them with plants for photosynthetically fixed carbon. This exchange is a significant factor in global nutrient cycles as well as in the ecology, evolution and physiology of plants. Despite its importance as a nutrient, very little is known about how AMF take up nitrogen and transfer it to their host plants. Here we report the results of stable isotope labelling experiments showing that inorganic nitrogen taken up by the fungus outside the roots is incorporated into amino acids, translocated from the extraradical to the intraradical mycelium as arginine, but transferred to the plant without carbon. Consistent with this mechanism, the genes of primary nitrogen assimilation are preferentially expressed in the extraradical tissues, whereas genes associated with arginine breakdown are more highly expressed in the intraradical mycelium. Strong changes in the expression of these genes in response to nitrogen availability and form also support the operation of this novel metabolic pathway in the arbuscular mycorrhizal symbiosis.
DOI: 10.1038/nature03610
PubMed: 15944705
Links to Exploration step
pubmed:15944705Le document en format XML
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Pfeffer</name></author><author><name sortKey="Jin, Hairu" sort="Jin, Hairu" uniqKey="Jin H" first="Hairu" last="Jin">Hairu Jin</name></author><author><name sortKey="Abubaker, Jehad" sort="Abubaker, Jehad" uniqKey="Abubaker J" first="Jehad" last="Abubaker">Jehad Abubaker</name></author><author><name sortKey="Douds, David D" sort="Douds, David D" uniqKey="Douds D" first="David D" last="Douds">David D. Douds</name></author><author><name sortKey="Allen, James W" sort="Allen, James W" uniqKey="Allen J" first="James W" last="Allen">James W. Allen</name></author><author><name sortKey="Bucking, Heike" sort="Bucking, Heike" uniqKey="Bucking H" first="Heike" last="Bücking">Heike Bücking</name></author><author><name sortKey="Lammers, Peter J" sort="Lammers, Peter J" uniqKey="Lammers P" first="Peter J" last="Lammers">Peter J. Lammers</name></author><author><name sortKey="Shachar Hill, Yair" sort="Shachar Hill, Yair" uniqKey="Shachar Hill Y" first="Yair" last="Shachar-Hill">Yair Shachar-Hill</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15944705</idno><idno type="pmid">15944705</idno><idno type="doi">10.1038/nature03610</idno><idno type="wicri:Area/Main/Corpus">003531</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003531</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Nitrogen transfer in the arbuscular mycorrhizal symbiosis.</title><author><name sortKey="Govindarajulu, Manjula" sort="Govindarajulu, Manjula" uniqKey="Govindarajulu M" first="Manjula" last="Govindarajulu">Manjula Govindarajulu</name><affiliation><nlm:affiliation>Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Pfeffer, Philip E" sort="Pfeffer, Philip E" uniqKey="Pfeffer P" first="Philip E" last="Pfeffer">Philip E. Pfeffer</name></author><author><name sortKey="Jin, Hairu" sort="Jin, Hairu" uniqKey="Jin H" first="Hairu" last="Jin">Hairu Jin</name></author><author><name sortKey="Abubaker, Jehad" sort="Abubaker, Jehad" uniqKey="Abubaker J" first="Jehad" last="Abubaker">Jehad Abubaker</name></author><author><name sortKey="Douds, David D" sort="Douds, David D" uniqKey="Douds D" first="David D" last="Douds">David D. Douds</name></author><author><name sortKey="Allen, James W" sort="Allen, James W" uniqKey="Allen J" first="James W" last="Allen">James W. Allen</name></author><author><name sortKey="Bucking, Heike" sort="Bucking, Heike" uniqKey="Bucking H" first="Heike" last="Bücking">Heike Bücking</name></author><author><name sortKey="Lammers, Peter J" sort="Lammers, Peter J" uniqKey="Lammers P" first="Peter J" last="Lammers">Peter J. Lammers</name></author><author><name sortKey="Shachar Hill, Yair" sort="Shachar Hill, Yair" uniqKey="Shachar Hill Y" first="Yair" last="Shachar-Hill">Yair Shachar-Hill</name></author></analytic><series><title level="j">Nature</title><idno type="eISSN">1476-4687</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetates (metabolism)</term><term>Amino Acids (MeSH)</term><term>Arginine (metabolism)</term><term>DNA, Bacterial (genetics)</term><term>Daucus carota (genetics)</term><term>Daucus carota (metabolism)</term><term>Daucus carota (microbiology)</term><term>Gene Expression Regulation (MeSH)</term><term>Genes, Fungal (genetics)</term><term>Genes, Plant (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycelium (metabolism)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (metabolism)</term><term>Nitrates (metabolism)</term><term>Nitrogen (metabolism)</term><term>Symbiosis (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acetates</term><term>Arginine</term><term>Nitrates</term><term>Nitrogen</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Amino Acids</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Daucus carota</term><term>Genes, Fungal</term><term>Genes, Plant</term><term>Mycorrhizae</term><term>Symbiosis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Daucus carota</term><term>Mycelium</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Daucus carota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation</term><term>Molecular Sequence Data</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Most land plants are symbiotic with arbuscular mycorrhizal fungi (AMF), which take up mineral nutrients from the soil and exchange them with plants for photosynthetically fixed carbon. This exchange is a significant factor in global nutrient cycles as well as in the ecology, evolution and physiology of plants. Despite its importance as a nutrient, very little is known about how AMF take up nitrogen and transfer it to their host plants. Here we report the results of stable isotope labelling experiments showing that inorganic nitrogen taken up by the fungus outside the roots is incorporated into amino acids, translocated from the extraradical to the intraradical mycelium as arginine, but transferred to the plant without carbon. Consistent with this mechanism, the genes of primary nitrogen assimilation are preferentially expressed in the extraradical tissues, whereas genes associated with arginine breakdown are more highly expressed in the intraradical mycelium. Strong changes in the expression of these genes in response to nitrogen availability and form also support the operation of this novel metabolic pathway in the arbuscular mycorrhizal symbiosis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15944705</PMID><DateCompleted><Year>2005</Year><Month>06</Month><Day>21</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1476-4687</ISSN><JournalIssue CitedMedium="Internet"><Volume>435</Volume><Issue>7043</Issue><PubDate><Year>2005</Year><Month>Jun</Month><Day>09</Day></PubDate></JournalIssue><Title>Nature</Title><ISOAbbreviation>Nature</ISOAbbreviation></Journal><ArticleTitle>Nitrogen transfer in the arbuscular mycorrhizal symbiosis.</ArticleTitle><Pagination><MedlinePgn>819-23</MedlinePgn></Pagination><Abstract><AbstractText>Most land plants are symbiotic with arbuscular mycorrhizal fungi (AMF), which take up mineral nutrients from the soil and exchange them with plants for photosynthetically fixed carbon. This exchange is a significant factor in global nutrient cycles as well as in the ecology, evolution and physiology of plants. Despite its importance as a nutrient, very little is known about how AMF take up nitrogen and transfer it to their host plants. Here we report the results of stable isotope labelling experiments showing that inorganic nitrogen taken up by the fungus outside the roots is incorporated into amino acids, translocated from the extraradical to the intraradical mycelium as arginine, but transferred to the plant without carbon. Consistent with this mechanism, the genes of primary nitrogen assimilation are preferentially expressed in the extraradical tissues, whereas genes associated with arginine breakdown are more highly expressed in the intraradical mycelium. Strong changes in the expression of these genes in response to nitrogen availability and form also support the operation of this novel metabolic pathway in the arbuscular mycorrhizal symbiosis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Govindarajulu</LastName><ForeName>Manjula</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pfeffer</LastName><ForeName>Philip E</ForeName><Initials>PE</Initials></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Hairu</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Abubaker</LastName><ForeName>Jehad</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Douds</LastName><ForeName>David D</ForeName><Initials>DD</Initials></Author><Author ValidYN="Y"><LastName>Allen</LastName><ForeName>James W</ForeName><Initials>JW</Initials></Author><Author ValidYN="Y"><LastName>Bücking</LastName><ForeName>Heike</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Lammers</LastName><ForeName>Peter J</ForeName><Initials>PJ</Initials></Author><Author ValidYN="Y"><LastName>Shachar-Hill</LastName><ForeName>Yair</ForeName><Initials>Y</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AY745984</AccessionNumber><AccessionNumber>BI452093</AccessionNumber><AccessionNumber>BI452207</AccessionNumber><AccessionNumber>CV186300</AccessionNumber><AccessionNumber>DQ063587</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, 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