Differential gene expressions in arbuscular mycorrhizal-colonized tomato grown under heavy metal stress.
Identifieur interne : 003502 ( Main/Corpus ); précédent : 003501; suivant : 003503Differential gene expressions in arbuscular mycorrhizal-colonized tomato grown under heavy metal stress.
Auteurs : Fouad Ouziad ; Ulrich Hildebrandt ; Elmon Schmelzer ; Hermann BotheSource :
- Journal of plant physiology [ 0176-1617 ] ; 2005.
English descriptors
- KwdEn :
- Cadmium Chloride (pharmacology), Gene Expression Regulation, Plant (MeSH), Lead (pharmacology), Lycopersicon esculentum (drug effects), Lycopersicon esculentum (metabolism), Lycopersicon esculentum (microbiology), Metals, Heavy (pharmacology), Mycorrhizae (metabolism), Nickel (pharmacology), Plant Leaves (metabolism), Plant Proteins (metabolism), Plant Roots (metabolism), Plant Roots (microbiology), Transcription, Genetic (MeSH), Zinc (pharmacology).
- MESH :
- chemical , metabolism : Plant Proteins.
- chemical , pharmacology : Cadmium Chloride, Lead, Metals, Heavy, Nickel, Zinc.
- drug effects : Lycopersicon esculentum.
- metabolism : Lycopersicon esculentum, Mycorrhizae, Plant Leaves, Plant Roots.
- microbiology : Lycopersicon esculentum, Plant Roots.
- Gene Expression Regulation, Plant, Transcription, Genetic.
Abstract
When tomato was grown in either "Breinigerberg" soil, which has a high content of Zn and of other heavy metals or in non-polluted soil enriched with up to 1 mM CdCl2, plants colonized with the arbuscular mycorrhizal fungus (AMF) Glomus intraradices grew distinctly better than non-mycorrhizal controls. An analysis of differential mRNA transcript formations was performed on several plant genes coding for products potentially involved in heavy metal tolerance. Northern blot analyses indicated that the mRNA from either roots or leaves was not differentially expressed in the case of LePCS1 (coding for phytochelatin synthase), Lemt1, Lemt3 and Lemt4 (for metallothioneins) or LeNramp2 (for a broad range heavy metal transporter) in both mycorrhizal and non-mycorrhizal plants, grown either with or without heavy metals. In contrast, Lemt2 was strongly expressed only in non-AMF-colonized roots, and only after growth in the Breinigerberg soil or in the presence of high CdCl2-concentrations. AMF colonization distinctly reduced the level of Lemt2 transcripts. This was also the case for the root specific LeNramp1 transporter, however, only after growth in the Breinigerberg soil, but not under Cd-stress. Likewise, the levels of LeNramp3 transcripts were reduced by the AMF colonization in roots, but not in leaves. Quantitative Real-Time RT-PCR-experiments performed with Lemt2, LeNramp1 and LeNramp3 largely corroborated the Northern analysis data. In situ hybridization experiments with Lemt2 and LeNramp1 showed that both genes were strongly expressed throughout the plant cells in non-colonized roots, whereas colonized roots revealed only few signals restricted to some parenchyma cells. All the data suggest that the transcript levels of some, but not all genes of the Nramp or mt family are elevated under heavy metal stress. AMF colonization results in a down-regulation of these genes, presumably due to the fact that the content of heavy metals is lower in mycorrhizal than in non-colonized roots. A suppression subtractive hybridization (SSH) Library from hyphae of the AMF G. intraradices grown in high versus low Zn++ provided none of the genes which were down-regulated at the plant side (mt or Nramp genes). In contrast, several gene sequences coding for enzymes potentially catalysing the detoxification of reactive oxygen species were found. Thus the fungal cells in the symbiosis may primarily have to cope with the heavy metal-induced oxidative stress.
DOI: 10.1016/j.jplph.2004.09.014
PubMed: 16008086
Links to Exploration step
pubmed:16008086Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Differential gene expressions in arbuscular mycorrhizal-colonized tomato grown under heavy metal stress.</title>
<author><name sortKey="Ouziad, Fouad" sort="Ouziad, Fouad" uniqKey="Ouziad F" first="Fouad" last="Ouziad">Fouad Ouziad</name>
<affiliation><nlm:affiliation>Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, D-50923 Köln, Germany.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hildebrandt, Ulrich" sort="Hildebrandt, Ulrich" uniqKey="Hildebrandt U" first="Ulrich" last="Hildebrandt">Ulrich Hildebrandt</name>
</author>
<author><name sortKey="Schmelzer, Elmon" sort="Schmelzer, Elmon" uniqKey="Schmelzer E" first="Elmon" last="Schmelzer">Elmon Schmelzer</name>
</author>
<author><name sortKey="Bothe, Hermann" sort="Bothe, Hermann" uniqKey="Bothe H" first="Hermann" last="Bothe">Hermann Bothe</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="RBID">pubmed:16008086</idno>
<idno type="pmid">16008086</idno>
<idno type="doi">10.1016/j.jplph.2004.09.014</idno>
<idno type="wicri:Area/Main/Corpus">003502</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003502</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Differential gene expressions in arbuscular mycorrhizal-colonized tomato grown under heavy metal stress.</title>
<author><name sortKey="Ouziad, Fouad" sort="Ouziad, Fouad" uniqKey="Ouziad F" first="Fouad" last="Ouziad">Fouad Ouziad</name>
<affiliation><nlm:affiliation>Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, D-50923 Köln, Germany.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Hildebrandt, Ulrich" sort="Hildebrandt, Ulrich" uniqKey="Hildebrandt U" first="Ulrich" last="Hildebrandt">Ulrich Hildebrandt</name>
</author>
<author><name sortKey="Schmelzer, Elmon" sort="Schmelzer, Elmon" uniqKey="Schmelzer E" first="Elmon" last="Schmelzer">Elmon Schmelzer</name>
</author>
<author><name sortKey="Bothe, Hermann" sort="Bothe, Hermann" uniqKey="Bothe H" first="Hermann" last="Bothe">Hermann Bothe</name>
</author>
</analytic>
<series><title level="j">Journal of plant physiology</title>
<idno type="ISSN">0176-1617</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cadmium Chloride (pharmacology)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Lead (pharmacology)</term>
<term>Lycopersicon esculentum (drug effects)</term>
<term>Lycopersicon esculentum (metabolism)</term>
<term>Lycopersicon esculentum (microbiology)</term>
<term>Metals, Heavy (pharmacology)</term>
<term>Mycorrhizae (metabolism)</term>
<term>Nickel (pharmacology)</term>
<term>Plant Leaves (metabolism)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plant Roots (metabolism)</term>
<term>Plant Roots (microbiology)</term>
<term>Transcription, Genetic (MeSH)</term>
<term>Zinc (pharmacology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cadmium Chloride</term>
<term>Lead</term>
<term>Metals, Heavy</term>
<term>Nickel</term>
<term>Zinc</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lycopersicon esculentum</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lycopersicon esculentum</term>
<term>Mycorrhizae</term>
<term>Plant Leaves</term>
<term>Plant Roots</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lycopersicon esculentum</term>
<term>Plant Roots</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
<term>Transcription, Genetic</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">When tomato was grown in either "Breinigerberg" soil, which has a high content of Zn and of other heavy metals or in non-polluted soil enriched with up to 1 mM CdCl2, plants colonized with the arbuscular mycorrhizal fungus (AMF) Glomus intraradices grew distinctly better than non-mycorrhizal controls. An analysis of differential mRNA transcript formations was performed on several plant genes coding for products potentially involved in heavy metal tolerance. Northern blot analyses indicated that the mRNA from either roots or leaves was not differentially expressed in the case of LePCS1 (coding for phytochelatin synthase), Lemt1, Lemt3 and Lemt4 (for metallothioneins) or LeNramp2 (for a broad range heavy metal transporter) in both mycorrhizal and non-mycorrhizal plants, grown either with or without heavy metals. In contrast, Lemt2 was strongly expressed only in non-AMF-colonized roots, and only after growth in the Breinigerberg soil or in the presence of high CdCl2-concentrations. AMF colonization distinctly reduced the level of Lemt2 transcripts. This was also the case for the root specific LeNramp1 transporter, however, only after growth in the Breinigerberg soil, but not under Cd-stress. Likewise, the levels of LeNramp3 transcripts were reduced by the AMF colonization in roots, but not in leaves. Quantitative Real-Time RT-PCR-experiments performed with Lemt2, LeNramp1 and LeNramp3 largely corroborated the Northern analysis data. In situ hybridization experiments with Lemt2 and LeNramp1 showed that both genes were strongly expressed throughout the plant cells in non-colonized roots, whereas colonized roots revealed only few signals restricted to some parenchyma cells. All the data suggest that the transcript levels of some, but not all genes of the Nramp or mt family are elevated under heavy metal stress. AMF colonization results in a down-regulation of these genes, presumably due to the fact that the content of heavy metals is lower in mycorrhizal than in non-colonized roots. A suppression subtractive hybridization (SSH) Library from hyphae of the AMF G. intraradices grown in high versus low Zn++ provided none of the genes which were down-regulated at the plant side (mt or Nramp genes). In contrast, several gene sequences coding for enzymes potentially catalysing the detoxification of reactive oxygen species were found. Thus the fungal cells in the symbiosis may primarily have to cope with the heavy metal-induced oxidative stress.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16008086</PMID>
<DateCompleted><Year>2005</Year>
<Month>08</Month>
<Day>10</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>09</Month>
<Day>30</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0176-1617</ISSN>
<JournalIssue CitedMedium="Print"><Volume>162</Volume>
<Issue>6</Issue>
<PubDate><Year>2005</Year>
<Month>Jun</Month>
</PubDate>
</JournalIssue>
<Title>Journal of plant physiology</Title>
<ISOAbbreviation>J Plant Physiol</ISOAbbreviation>
</Journal>
<ArticleTitle>Differential gene expressions in arbuscular mycorrhizal-colonized tomato grown under heavy metal stress.</ArticleTitle>
<Pagination><MedlinePgn>634-49</MedlinePgn>
</Pagination>
<Abstract><AbstractText>When tomato was grown in either "Breinigerberg" soil, which has a high content of Zn and of other heavy metals or in non-polluted soil enriched with up to 1 mM CdCl2, plants colonized with the arbuscular mycorrhizal fungus (AMF) Glomus intraradices grew distinctly better than non-mycorrhizal controls. An analysis of differential mRNA transcript formations was performed on several plant genes coding for products potentially involved in heavy metal tolerance. Northern blot analyses indicated that the mRNA from either roots or leaves was not differentially expressed in the case of LePCS1 (coding for phytochelatin synthase), Lemt1, Lemt3 and Lemt4 (for metallothioneins) or LeNramp2 (for a broad range heavy metal transporter) in both mycorrhizal and non-mycorrhizal plants, grown either with or without heavy metals. In contrast, Lemt2 was strongly expressed only in non-AMF-colonized roots, and only after growth in the Breinigerberg soil or in the presence of high CdCl2-concentrations. AMF colonization distinctly reduced the level of Lemt2 transcripts. This was also the case for the root specific LeNramp1 transporter, however, only after growth in the Breinigerberg soil, but not under Cd-stress. Likewise, the levels of LeNramp3 transcripts were reduced by the AMF colonization in roots, but not in leaves. Quantitative Real-Time RT-PCR-experiments performed with Lemt2, LeNramp1 and LeNramp3 largely corroborated the Northern analysis data. In situ hybridization experiments with Lemt2 and LeNramp1 showed that both genes were strongly expressed throughout the plant cells in non-colonized roots, whereas colonized roots revealed only few signals restricted to some parenchyma cells. All the data suggest that the transcript levels of some, but not all genes of the Nramp or mt family are elevated under heavy metal stress. AMF colonization results in a down-regulation of these genes, presumably due to the fact that the content of heavy metals is lower in mycorrhizal than in non-colonized roots. A suppression subtractive hybridization (SSH) Library from hyphae of the AMF G. intraradices grown in high versus low Zn++ provided none of the genes which were down-regulated at the plant side (mt or Nramp genes). In contrast, several gene sequences coding for enzymes potentially catalysing the detoxification of reactive oxygen species were found. Thus the fungal cells in the symbiosis may primarily have to cope with the heavy metal-induced oxidative stress.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ouziad</LastName>
<ForeName>Fouad</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, D-50923 Köln, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hildebrandt</LastName>
<ForeName>Ulrich</ForeName>
<Initials>U</Initials>
</Author>
<Author ValidYN="Y"><LastName>Schmelzer</LastName>
<ForeName>Elmon</ForeName>
<Initials>E</Initials>
</Author>
<Author ValidYN="Y"><LastName>Bothe</LastName>
<ForeName>Hermann</ForeName>
<Initials>H</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>Germany</Country>
<MedlineTA>J Plant Physiol</MedlineTA>
<NlmUniqueID>9882059</NlmUniqueID>
<ISSNLinking>0176-1617</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>2P299V784P</RegistryNumber>
<NameOfSubstance UI="D007854">Lead</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>7OV03QG267</RegistryNumber>
<NameOfSubstance UI="D009532">Nickel</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>J41CSQ7QDS</RegistryNumber>
<NameOfSubstance UI="D015032">Zinc</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>J6K4F9V3BA</RegistryNumber>
<NameOfSubstance UI="D019256">Cadmium Chloride</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D019256" MajorTopicYN="N">Cadmium Chloride</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007854" MajorTopicYN="N">Lead</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019216" MajorTopicYN="N">Metals, Heavy</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009532" MajorTopicYN="N">Nickel</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015032" MajorTopicYN="N">Zinc</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>7</Month>
<Day>13</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2005</Year>
<Month>8</Month>
<Day>11</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2005</Year>
<Month>7</Month>
<Day>13</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16008086</ArticleId>
<ArticleId IdType="pii">S0176-1617(04)00244-5</ArticleId>
<ArticleId IdType="doi">10.1016/j.jplph.2004.09.014</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MycorrhizaeV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003502 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 003502 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= MycorrhizaeV1 |flux= Main |étape= Corpus |type= RBID |clé= pubmed:16008086 |texte= Differential gene expressions in arbuscular mycorrhizal-colonized tomato grown under heavy metal stress. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:16008086" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a MycorrhizaeV1
![]() | This area was generated with Dilib version V0.6.37. | ![]() |