Morphological, ontogenetic and molecular characterization of Scutellospora reticulata (Glomeromycota).
Identifieur interne : 003494 ( Main/Corpus ); précédent : 003493; suivant : 003495Morphological, ontogenetic and molecular characterization of Scutellospora reticulata (Glomeromycota).
Auteurs : Francisco Adriano De Souza ; Stéphane Declerck ; Eric Smit ; George A. KowalchukSource :
- Mycological research [ 0953-7562 ] ; 2005.
English descriptors
- KwdEn :
- DNA, Fungal (genetics), DNA, Ribosomal (genetics), Genetic Markers (MeSH), Molecular Sequence Data (MeSH), Mycorrhizae (classification), Mycorrhizae (cytology), Mycorrhizae (physiology), Phylogeny (MeSH), Polymorphism, Genetic (MeSH), Sequence Alignment (MeSH), Sequence Analysis (MeSH), Species Specificity (MeSH), Spores, Fungal (cytology), Spores, Fungal (growth & development).
- MESH :
- chemical , genetics : DNA, Fungal, DNA, Ribosomal.
- chemical : Genetic Markers.
- classification : Mycorrhizae.
- cytology : Mycorrhizae, Spores, Fungal.
- growth & development : Spores, Fungal.
- physiology : Mycorrhizae.
- Molecular Sequence Data, Phylogeny, Polymorphism, Genetic, Sequence Alignment, Sequence Analysis, Species Specificity.
Abstract
The arbuscular mycorrhizal (AM) fungus Scutellospora reticulata (CNPAB11) was characterized using morphological, ontogenetic and molecular approaches. Spore ontogenesis was studied using Ri T-DNA transformed carrot roots and observations were compared with those published for eight other, pot-cultured, Scutellospora species. The sporogenesis of S. reticulata exhibited an unreported pattern of outer spore wall differentiation. In addition, Denaturing Gradient Gel Electrophoresis (DGGE), targeting the V9 region of the SSU nrDNA, was used to differentiate S. reticulata from 16 other Scutellospora species and results were confirmed by sequencing analysis. Phylogenetic analyses, using nearly full length SSU nrDNA sequences, grouped S. reticulata in a cluster together with S. cerradensis and S. heterogama, species that share similar spore wall organization and also possess ornamented external walls. PCR-DGGE and sequence analysis revealed intragenomic SSU nrDNA polymorphisms in four out of six Scutellospora species tested, and demonstrated that SSU nrDNA intragenomic polymorphism could be used as a marker to differentiate several closely related Scutellospora species.
DOI: 10.1017/s0953756205002546
PubMed: 16080392
Links to Exploration step
pubmed:16080392Le document en format XML
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<affiliation><nlm:affiliation>Empresa Brasileira de Pesquisa Agropecuária, Embrapa Agrobiologia (CNPAB), Caixa Postal 74505, CEP 23851-970, Rodovia BR 465-RJ, km 7, Seropédica, Rio de Janeiro State, Brazil. fdesouza@cnpab.embrapa.br</nlm:affiliation>
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<author><name sortKey="Declerck, Stephane" sort="Declerck, Stephane" uniqKey="Declerck S" first="Stéphane" last="Declerck">Stéphane Declerck</name>
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<author><name sortKey="Smit, Eric" sort="Smit, Eric" uniqKey="Smit E" first="Eric" last="Smit">Eric Smit</name>
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<author><name sortKey="Kowalchuk, George A" sort="Kowalchuk, George A" uniqKey="Kowalchuk G" first="George A" last="Kowalchuk">George A. Kowalchuk</name>
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<front><div type="abstract" xml:lang="en">The arbuscular mycorrhizal (AM) fungus Scutellospora reticulata (CNPAB11) was characterized using morphological, ontogenetic and molecular approaches. Spore ontogenesis was studied using Ri T-DNA transformed carrot roots and observations were compared with those published for eight other, pot-cultured, Scutellospora species. The sporogenesis of S. reticulata exhibited an unreported pattern of outer spore wall differentiation. In addition, Denaturing Gradient Gel Electrophoresis (DGGE), targeting the V9 region of the SSU nrDNA, was used to differentiate S. reticulata from 16 other Scutellospora species and results were confirmed by sequencing analysis. Phylogenetic analyses, using nearly full length SSU nrDNA sequences, grouped S. reticulata in a cluster together with S. cerradensis and S. heterogama, species that share similar spore wall organization and also possess ornamented external walls. PCR-DGGE and sequence analysis revealed intragenomic SSU nrDNA polymorphisms in four out of six Scutellospora species tested, and demonstrated that SSU nrDNA intragenomic polymorphism could be used as a marker to differentiate several closely related Scutellospora species.</div>
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<Abstract><AbstractText>The arbuscular mycorrhizal (AM) fungus Scutellospora reticulata (CNPAB11) was characterized using morphological, ontogenetic and molecular approaches. Spore ontogenesis was studied using Ri T-DNA transformed carrot roots and observations were compared with those published for eight other, pot-cultured, Scutellospora species. The sporogenesis of S. reticulata exhibited an unreported pattern of outer spore wall differentiation. In addition, Denaturing Gradient Gel Electrophoresis (DGGE), targeting the V9 region of the SSU nrDNA, was used to differentiate S. reticulata from 16 other Scutellospora species and results were confirmed by sequencing analysis. Phylogenetic analyses, using nearly full length SSU nrDNA sequences, grouped S. reticulata in a cluster together with S. cerradensis and S. heterogama, species that share similar spore wall organization and also possess ornamented external walls. PCR-DGGE and sequence analysis revealed intragenomic SSU nrDNA polymorphisms in four out of six Scutellospora species tested, and demonstrated that SSU nrDNA intragenomic polymorphism could be used as a marker to differentiate several closely related Scutellospora species.</AbstractText>
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