The uptake, metabolism, transport and transfer of nitrogen in an arbuscular mycorrhizal symbiosis.
Identifieur interne : 003403 ( Main/Corpus ); précédent : 003402; suivant : 003404The uptake, metabolism, transport and transfer of nitrogen in an arbuscular mycorrhizal symbiosis.
Auteurs : H. Jin ; P E Pfeffer ; D D Douds ; E. Piotrowski ; P J Lammers ; Y. Shachar-HillSource :
- The New phytologist [ 0028-646X ] ; 2005.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Plant Proteins.
- chemical , metabolism : Amino Acids, Nitrogen, Plant Proteins.
- metabolism : Mycorrhizae.
- microbiology : Daucus carota.
- Biological Transport, Symbiosis.
Abstract
Nitrogen (N) is known to be transferred from fungus to plant in the arbuscular mycorrhizal (AM) symbiosis, yet its metabolism, storage and transport are poorly understood. In vitro mycorrhizas of Glomus intra-radices and Ri T-DNA-transformed carrot roots were grown in two-compartment Petri dishes. (15)N- and/or (13)C-labeled substrates were supplied to either the fungal compartment or to separate dishes containing uncolonized roots. The levels and labeling of free amino acids (AAs) in the extra-radical mycelium (ERM) in mycorrhizal roots and in uncolonized roots were measured by gas chromatography/mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). Arginine (Arg) was the predominant free AA in the ERM, and almost all Arg molecules became labeled within 3 wk of supplying (15)NH(4) (+) to the fungal compartment. Labeling in Arg represented > 90% of the total (15)N in the free AAs of the ERM. [Guanido-2-(15)N]Arg taken up by the ERM and transported to the intra-radical mycelium (IRM) gave rise to (15)N-labeled AAs. [U-(13)C]Arg added to the fungal compartment did not produce any (13)C labeling of other AAs in the mycorrhizal root. Arg is the major form of N synthesized and stored in the ERM and transported to the IRM. However, NH(4) (+) is the most likely form of N transferred to host cells following its generation from Arg breakdown.
DOI: 10.1111/j.1469-8137.2005.01536.x
PubMed: 16313650
Links to Exploration step
pubmed:16313650Le document en format XML
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Piotrowski</name></author><author><name sortKey="Lammers, P J" sort="Lammers, P J" uniqKey="Lammers P" first="P J" last="Lammers">P J Lammers</name></author><author><name sortKey="Shachar Hill, Y" sort="Shachar Hill, Y" uniqKey="Shachar Hill Y" first="Y" last="Shachar-Hill">Y. Shachar-Hill</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16313650</idno><idno type="pmid">16313650</idno><idno type="doi">10.1111/j.1469-8137.2005.01536.x</idno><idno type="wicri:Area/Main/Corpus">003403</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003403</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The uptake, metabolism, transport and transfer of nitrogen in an arbuscular mycorrhizal symbiosis.</title><author><name sortKey="Jin, H" sort="Jin, H" uniqKey="Jin H" first="H" last="Jin">H. Jin</name><affiliation><nlm:affiliation>USDA, ARS, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Pfeffer, P E" sort="Pfeffer, P E" uniqKey="Pfeffer P" first="P E" last="Pfeffer">P E Pfeffer</name></author><author><name sortKey="Douds, D D" sort="Douds, D D" uniqKey="Douds D" first="D D" last="Douds">D D Douds</name></author><author><name sortKey="Piotrowski, E" sort="Piotrowski, E" uniqKey="Piotrowski E" first="E" last="Piotrowski">E. Piotrowski</name></author><author><name sortKey="Lammers, P J" sort="Lammers, P J" uniqKey="Lammers P" first="P J" last="Lammers">P J Lammers</name></author><author><name sortKey="Shachar Hill, Y" sort="Shachar Hill, Y" uniqKey="Shachar Hill Y" first="Y" last="Shachar-Hill">Y. Shachar-Hill</name></author></analytic><series><title level="j">The New phytologist</title><idno type="ISSN">0028-646X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids (metabolism)</term><term>Biological Transport (MeSH)</term><term>Daucus carota (microbiology)</term><term>Mycorrhizae (metabolism)</term><term>Nitrogen (metabolism)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (metabolism)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amino Acids</term><term>Nitrogen</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Daucus carota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Transport</term><term>Symbiosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nitrogen (N) is known to be transferred from fungus to plant in the arbuscular mycorrhizal (AM) symbiosis, yet its metabolism, storage and transport are poorly understood. In vitro mycorrhizas of Glomus intra-radices and Ri T-DNA-transformed carrot roots were grown in two-compartment Petri dishes. (15)N- and/or (13)C-labeled substrates were supplied to either the fungal compartment or to separate dishes containing uncolonized roots. The levels and labeling of free amino acids (AAs) in the extra-radical mycelium (ERM) in mycorrhizal roots and in uncolonized roots were measured by gas chromatography/mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). Arginine (Arg) was the predominant free AA in the ERM, and almost all Arg molecules became labeled within 3 wk of supplying (15)NH(4) (+) to the fungal compartment. Labeling in Arg represented > 90% of the total (15)N in the free AAs of the ERM. [Guanido-2-(15)N]Arg taken up by the ERM and transported to the intra-radical mycelium (IRM) gave rise to (15)N-labeled AAs. [U-(13)C]Arg added to the fungal compartment did not produce any (13)C labeling of other AAs in the mycorrhizal root. Arg is the major form of N synthesized and stored in the ERM and transported to the IRM. However, NH(4) (+) is the most likely form of N transferred to host cells following its generation from Arg breakdown.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16313650</PMID><DateCompleted><Year>2006</Year><Month>04</Month><Day>25</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0028-646X</ISSN><JournalIssue CitedMedium="Print"><Volume>168</Volume><Issue>3</Issue><PubDate><Year>2005</Year><Month>Dec</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>The uptake, metabolism, transport and transfer of nitrogen in an arbuscular mycorrhizal symbiosis.</ArticleTitle><Pagination><MedlinePgn>687-96</MedlinePgn></Pagination><Abstract><AbstractText>Nitrogen (N) is known to be transferred from fungus to plant in the arbuscular mycorrhizal (AM) symbiosis, yet its metabolism, storage and transport are poorly understood. In vitro mycorrhizas of Glomus intra-radices and Ri T-DNA-transformed carrot roots were grown in two-compartment Petri dishes. (15)N- and/or (13)C-labeled substrates were supplied to either the fungal compartment or to separate dishes containing uncolonized roots. The levels and labeling of free amino acids (AAs) in the extra-radical mycelium (ERM) in mycorrhizal roots and in uncolonized roots were measured by gas chromatography/mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). Arginine (Arg) was the predominant free AA in the ERM, and almost all Arg molecules became labeled within 3 wk of supplying (15)NH(4) (+) to the fungal compartment. Labeling in Arg represented > 90% of the total (15)N in the free AAs of the ERM. [Guanido-2-(15)N]Arg taken up by the ERM and transported to the intra-radical mycelium (IRM) gave rise to (15)N-labeled AAs. [U-(13)C]Arg added to the fungal compartment did not produce any (13)C labeling of other AAs in the mycorrhizal root. Arg is the major form of N synthesized and stored in the ERM and transported to the IRM. However, NH(4) (+) is the most likely form of N transferred to host cells following its generation from Arg breakdown.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>USDA, ARS, Eastern Regional Research Center, 600 E. 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