Colonization by the arbuscular mycorrhizal fungus Glomus versiforme induces a defense response against the root-knot nematode Meloidogyne incognita in the grapevine (Vitis amurensis Rupr.), which includes transcriptional activation of the class III chitinase gene VCH3.
Identifieur interne : 003396 ( Main/Corpus ); précédent : 003395; suivant : 003397Colonization by the arbuscular mycorrhizal fungus Glomus versiforme induces a defense response against the root-knot nematode Meloidogyne incognita in the grapevine (Vitis amurensis Rupr.), which includes transcriptional activation of the class III chitinase gene VCH3.
Auteurs : Hai-Yan Li ; Guo-Dong Yang ; Huai-Rui Shu ; Yu-Tao Yang ; Bao-Xing Ye ; Ikuo Nishida ; Cheng-Chao ZhengSource :
- Plant & cell physiology [ 0032-0781 ] ; 2006.
English descriptors
- KwdEn :
- Animals (MeSH), Base Sequence (MeSH), Chitinases (classification), Chitinases (genetics), DNA, Plant (genetics), Genes, Plant (MeSH), Glucuronidase (genetics), Molecular Sequence Data (MeSH), Mycorrhizae (physiology), Plant Diseases (genetics), Plant Diseases (parasitology), Plants, Genetically Modified (MeSH), Promoter Regions, Genetic (MeSH), Symbiosis (MeSH), Tobacco (enzymology), Tobacco (genetics), Tobacco (microbiology), Tobacco (parasitology), Transcriptional Activation (MeSH), Tylenchoidea (pathogenicity), Vitis (enzymology), Vitis (genetics), Vitis (microbiology), Vitis (parasitology).
- MESH :
- chemical , classification : Chitinases.
- chemical , genetics : Chitinases, DNA, Plant, Glucuronidase.
- enzymology : Tobacco, Vitis.
- genetics : Plant Diseases, Tobacco, Vitis.
- microbiology : Tobacco, Vitis.
- parasitology : Plant Diseases, Tobacco, Vitis.
- pathogenicity : Tylenchoidea.
- physiology : Mycorrhizae.
- Animals, Base Sequence, Genes, Plant, Molecular Sequence Data, Plants, Genetically Modified, Promoter Regions, Genetic, Symbiosis, Transcriptional Activation.
Abstract
Inoculation of the grapevine (Vitis amurensis Rupr.) with the arbuscular mycorrhizal (AM) fungus Glomus versiforme significantly increased resistance against the root-knot nematode (RKN) Meloidogyne incognita. Studies using relative quantitative reverse transcription-PCR (RQRT-PCR) analysis of grapevine root inoculation with the AM fungus revealed an up-regulation of VCH3 transcripts. This increase was greater than that observed following infection with RKN. However, inoculation of the mycorrhizal grapevine roots with RKN was able to enhance VCH3 transcript expression further. Moreover, the increase in VCH3 transcripts appeared to result in a higher level of resistance against subsequent RKN infection. Constitutive expression of VCH3 cDNA in transgenic tobacco under the control of the cauliflower mosaic virus 35S promoter also conferred resistance against RKN, but had no significant effect on the growth of the AM fungus. We analyzed beta-glucuronidase (GUS) activity directed by a 1,216 bp VCH3 promoter in transgenic tobacco following inoculation with both the AM fungus and RKN. GUS activity was negligible in the root tissues before inoculation, and was more effectively induced after inoculation with the AM fungus than with RKN. Moreover, GUS staining in the mycorrhizal transgenic tobacco roots was enhanced by subsequent RKN infection, and was found ubiquitously throughout the whole root tissue. Together, these results suggest that AM fungus induced a defense response against RKN in the mycorrhizal grapevine roots, which appeared to involve transcriptional control of VCH3 expression throughout the whole root tissue.
DOI: 10.1093/pcp/pci231
PubMed: 16326755
Links to Exploration step
pubmed:16326755Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Colonization by the arbuscular mycorrhizal fungus Glomus versiforme induces a defense response against the root-knot nematode Meloidogyne incognita in the grapevine (Vitis amurensis Rupr.), which includes transcriptional activation of the class III chitinase gene VCH3.</title><author><name sortKey="Li, Hai Yan" sort="Li, Hai Yan" uniqKey="Li H" first="Hai-Yan" last="Li">Hai-Yan Li</name><affiliation><nlm:affiliation>College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Guo Dong" sort="Yang, Guo Dong" uniqKey="Yang G" first="Guo-Dong" last="Yang">Guo-Dong Yang</name></author><author><name sortKey="Shu, Huai Rui" sort="Shu, Huai Rui" uniqKey="Shu H" first="Huai-Rui" last="Shu">Huai-Rui Shu</name></author><author><name sortKey="Yang, Yu Tao" sort="Yang, Yu Tao" uniqKey="Yang Y" first="Yu-Tao" last="Yang">Yu-Tao Yang</name></author><author><name sortKey="Ye, Bao Xing" sort="Ye, Bao Xing" uniqKey="Ye B" first="Bao-Xing" last="Ye">Bao-Xing Ye</name></author><author><name sortKey="Nishida, Ikuo" sort="Nishida, Ikuo" uniqKey="Nishida I" first="Ikuo" last="Nishida">Ikuo Nishida</name></author><author><name sortKey="Zheng, Cheng Chao" sort="Zheng, Cheng Chao" uniqKey="Zheng C" first="Cheng-Chao" last="Zheng">Cheng-Chao Zheng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16326755</idno><idno type="pmid">16326755</idno><idno type="doi">10.1093/pcp/pci231</idno><idno type="wicri:Area/Main/Corpus">003396</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003396</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Colonization by the arbuscular mycorrhizal fungus Glomus versiforme induces a defense response against the root-knot nematode Meloidogyne incognita in the grapevine (Vitis amurensis Rupr.), which includes transcriptional activation of the class III chitinase gene VCH3.</title><author><name sortKey="Li, Hai Yan" sort="Li, Hai Yan" uniqKey="Li H" first="Hai-Yan" last="Li">Hai-Yan Li</name><affiliation><nlm:affiliation>College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Guo Dong" sort="Yang, Guo Dong" uniqKey="Yang G" first="Guo-Dong" last="Yang">Guo-Dong Yang</name></author><author><name sortKey="Shu, Huai Rui" sort="Shu, Huai Rui" uniqKey="Shu H" first="Huai-Rui" last="Shu">Huai-Rui Shu</name></author><author><name sortKey="Yang, Yu Tao" sort="Yang, Yu Tao" uniqKey="Yang Y" first="Yu-Tao" last="Yang">Yu-Tao Yang</name></author><author><name sortKey="Ye, Bao Xing" sort="Ye, Bao Xing" uniqKey="Ye B" first="Bao-Xing" last="Ye">Bao-Xing Ye</name></author><author><name sortKey="Nishida, Ikuo" sort="Nishida, Ikuo" uniqKey="Nishida I" first="Ikuo" last="Nishida">Ikuo Nishida</name></author><author><name sortKey="Zheng, Cheng Chao" sort="Zheng, Cheng Chao" uniqKey="Zheng C" first="Cheng-Chao" last="Zheng">Cheng-Chao Zheng</name></author></analytic><series><title level="j">Plant & cell physiology</title><idno type="ISSN">0032-0781</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Base Sequence (MeSH)</term><term>Chitinases (classification)</term><term>Chitinases (genetics)</term><term>DNA, Plant (genetics)</term><term>Genes, Plant (MeSH)</term><term>Glucuronidase (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (physiology)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (parasitology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Symbiosis (MeSH)</term><term>Tobacco (enzymology)</term><term>Tobacco (genetics)</term><term>Tobacco (microbiology)</term><term>Tobacco (parasitology)</term><term>Transcriptional Activation (MeSH)</term><term>Tylenchoidea (pathogenicity)</term><term>Vitis (enzymology)</term><term>Vitis (genetics)</term><term>Vitis (microbiology)</term><term>Vitis (parasitology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Chitinases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chitinases</term><term>DNA, Plant</term><term>Glucuronidase</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Tobacco</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Diseases</term><term>Tobacco</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Tobacco</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term><term>Tobacco</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Tylenchoidea</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Genes, Plant</term><term>Molecular Sequence Data</term><term>Plants, Genetically Modified</term><term>Promoter Regions, Genetic</term><term>Symbiosis</term><term>Transcriptional Activation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Inoculation of the grapevine (Vitis amurensis Rupr.) with the arbuscular mycorrhizal (AM) fungus Glomus versiforme significantly increased resistance against the root-knot nematode (RKN) Meloidogyne incognita. Studies using relative quantitative reverse transcription-PCR (RQRT-PCR) analysis of grapevine root inoculation with the AM fungus revealed an up-regulation of VCH3 transcripts. This increase was greater than that observed following infection with RKN. However, inoculation of the mycorrhizal grapevine roots with RKN was able to enhance VCH3 transcript expression further. Moreover, the increase in VCH3 transcripts appeared to result in a higher level of resistance against subsequent RKN infection. Constitutive expression of VCH3 cDNA in transgenic tobacco under the control of the cauliflower mosaic virus 35S promoter also conferred resistance against RKN, but had no significant effect on the growth of the AM fungus. We analyzed beta-glucuronidase (GUS) activity directed by a 1,216 bp VCH3 promoter in transgenic tobacco following inoculation with both the AM fungus and RKN. GUS activity was negligible in the root tissues before inoculation, and was more effectively induced after inoculation with the AM fungus than with RKN. Moreover, GUS staining in the mycorrhizal transgenic tobacco roots was enhanced by subsequent RKN infection, and was found ubiquitously throughout the whole root tissue. Together, these results suggest that AM fungus induced a defense response against RKN in the mycorrhizal grapevine roots, which appeared to involve transcriptional control of VCH3 expression throughout the whole root tissue.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16326755</PMID><DateCompleted><Year>2006</Year><Month>04</Month><Day>10</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0032-0781</ISSN><JournalIssue CitedMedium="Print"><Volume>47</Volume><Issue>1</Issue><PubDate><Year>2006</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant & cell physiology</Title><ISOAbbreviation>Plant Cell Physiol</ISOAbbreviation></Journal><ArticleTitle>Colonization by the arbuscular mycorrhizal fungus Glomus versiforme induces a defense response against the root-knot nematode Meloidogyne incognita in the grapevine (Vitis amurensis Rupr.), which includes transcriptional activation of the class III chitinase gene VCH3.</ArticleTitle><Pagination><MedlinePgn>154-63</MedlinePgn></Pagination><Abstract><AbstractText>Inoculation of the grapevine (Vitis amurensis Rupr.) with the arbuscular mycorrhizal (AM) fungus Glomus versiforme significantly increased resistance against the root-knot nematode (RKN) Meloidogyne incognita. Studies using relative quantitative reverse transcription-PCR (RQRT-PCR) analysis of grapevine root inoculation with the AM fungus revealed an up-regulation of VCH3 transcripts. This increase was greater than that observed following infection with RKN. However, inoculation of the mycorrhizal grapevine roots with RKN was able to enhance VCH3 transcript expression further. Moreover, the increase in VCH3 transcripts appeared to result in a higher level of resistance against subsequent RKN infection. Constitutive expression of VCH3 cDNA in transgenic tobacco under the control of the cauliflower mosaic virus 35S promoter also conferred resistance against RKN, but had no significant effect on the growth of the AM fungus. We analyzed beta-glucuronidase (GUS) activity directed by a 1,216 bp VCH3 promoter in transgenic tobacco following inoculation with both the AM fungus and RKN. GUS activity was negligible in the root tissues before inoculation, and was more effectively induced after inoculation with the AM fungus than with RKN. Moreover, GUS staining in the mycorrhizal transgenic tobacco roots was enhanced by subsequent RKN infection, and was found ubiquitously throughout the whole root tissue. Together, these results suggest that AM fungus induced a defense response against RKN in the mycorrhizal grapevine roots, which appeared to involve transcriptional control of VCH3 expression throughout the whole root tissue.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Hai-Yan</ForeName><Initials>HY</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Guo-Dong</ForeName><Initials>GD</Initials></Author><Author ValidYN="Y"><LastName>Shu</LastName><ForeName>Huai-Rui</ForeName><Initials>HR</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yu-Tao</ForeName><Initials>YT</Initials></Author><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Bao-Xing</ForeName><Initials>BX</Initials></Author><Author ValidYN="Y"><LastName>Nishida</LastName><ForeName>Ikuo</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Cheng-Chao</ForeName><Initials>CC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>12</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>Plant Cell Physiol</MedlineTA><NlmUniqueID>9430925</NlmUniqueID><ISSNLinking>0032-0781</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.14</RegistryNumber><NameOfSubstance UI="D002688">Chitinases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002688" MajorTopicYN="N">Chitinases</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015533" MajorTopicYN="N">Transcriptional Activation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014431" MajorTopicYN="N">Tylenchoidea</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027843" MajorTopicYN="N">Vitis</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>12</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>4</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>12</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16326755</ArticleId><ArticleId IdType="pii">pci231</ArticleId><ArticleId IdType="doi">10.1093/pcp/pci231</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MycorrhizaeV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003396 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 003396 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MycorrhizaeV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:16326755
|texte= Colonization by the arbuscular mycorrhizal fungus Glomus versiforme induces a defense response against the root-knot nematode Meloidogyne incognita in the grapevine (Vitis amurensis Rupr.), which includes transcriptional activation of the class III chitinase gene VCH3.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:16326755" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a MycorrhizaeV1
| This area was generated with Dilib version V0.6.37. |  |