Colonisation of Pinus halepensis roots by Pseudomonas fluorescens and interaction with the ectomycorrhizal fungus Suillus granulatus.
Identifieur interne : 003394 ( Main/Corpus ); précédent : 003393; suivant : 003395Colonisation of Pinus halepensis roots by Pseudomonas fluorescens and interaction with the ectomycorrhizal fungus Suillus granulatus.
Auteurs : Ana Rinc N ; Beatriz Ruiz-Díez ; Sonia García-Fraile ; José Antonio Lucas García ; Mercedes Fernández-Pascual ; José J. Pueyo ; María R. De FelipeSource :
- FEMS microbiology ecology [ 0168-6496 ] ; 2005.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Basidiomycota (growth & development), Colony Count, Microbial (MeSH), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Mycorrhizae (MeSH), Pinus (growth & development), Pinus (microbiology), Plant Roots (microbiology), Pseudomonas fluorescens (genetics), Pseudomonas fluorescens (growth & development), Pseudomonas fluorescens (metabolism), Soil Microbiology (MeSH).
- MESH :
- chemical , genetics : Bacterial Proteins, Green Fluorescent Proteins.
- chemical , metabolism : Bacterial Proteins, Green Fluorescent Proteins.
- genetics : Pseudomonas fluorescens.
- growth & development : Basidiomycota, Pinus, Pseudomonas fluorescens.
- metabolism : Pseudomonas fluorescens.
- microbiology : Pinus, Plant Roots.
- Colony Count, Microbial, Mycorrhizae, Soil Microbiology.
Abstract
Colonisation of Pinus halepensis roots by GFP-tagged Pseudomonas fluorescens Aur6 was monitored by epifluorescence microscopy and dilution plating. Aur6-GFP was able to colonise and proliferate on P. halepensis roots. Co-inoculation with the ectomycorrhizal fungus Suillus granulatus did not affect the bacterial colonisation pattern whereas it had an effect on bacterial density. Bacterial counts increased during the first 20 days of seedling growth, irrespective of seedlings being mycorrhizal or not. After 40 days, bacterial density significantly decreased and bacteria concentrated on the upper two-thirds of the pine root. The presence of S. granulatus significantly stimulated survival of bacteria in the root elongation zone where fungal colonisation was higher. The number of mycorrhizas formed by S. granulatus was not affected by co-inoculation with Aur6-GFP. Neither Aur6-GFP nor S. granulatus stimulated P. halepensis development when inoculated alone, but a synergistic effect was observed on seedling growth when bacteria and fungus were co-inoculated.
DOI: 10.1016/j.femsec.2004.09.006
PubMed: 16329878
Links to Exploration step
pubmed:16329878Le document en format XML
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<author><name sortKey="Rinc N, Ana" sort="Rinc N, Ana" uniqKey="Rinc N A" first="Ana" last="Rinc N">Ana Rinc N</name>
<affiliation><nlm:affiliation>Department of Plant Physiology and Biochemistry, Centro de Ciencias Medioambientales, CSIC, Serrano, 115-bis, 28006 Madrid, Spain. ana.rincon@ccma.csic.es</nlm:affiliation>
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<author><name sortKey="Ruiz Diez, Beatriz" sort="Ruiz Diez, Beatriz" uniqKey="Ruiz Diez B" first="Beatriz" last="Ruiz-Díez">Beatriz Ruiz-Díez</name>
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<author><name sortKey="Garcia Fraile, Sonia" sort="Garcia Fraile, Sonia" uniqKey="Garcia Fraile S" first="Sonia" last="García-Fraile">Sonia García-Fraile</name>
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<author><name sortKey="Garcia, Jose Antonio Lucas" sort="Garcia, Jose Antonio Lucas" uniqKey="Garcia J" first="José Antonio Lucas" last="García">José Antonio Lucas García</name>
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<author><name sortKey="Fernandez Pascual, Mercedes" sort="Fernandez Pascual, Mercedes" uniqKey="Fernandez Pascual M" first="Mercedes" last="Fernández-Pascual">Mercedes Fernández-Pascual</name>
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<author><name sortKey="Pueyo, Jose J" sort="Pueyo, Jose J" uniqKey="Pueyo J" first="José J" last="Pueyo">José J. Pueyo</name>
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<author><name sortKey="De Felipe, Maria R" sort="De Felipe, Maria R" uniqKey="De Felipe M" first="María R" last="De Felipe">María R. De Felipe</name>
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<affiliation><nlm:affiliation>Department of Plant Physiology and Biochemistry, Centro de Ciencias Medioambientales, CSIC, Serrano, 115-bis, 28006 Madrid, Spain. ana.rincon@ccma.csic.es</nlm:affiliation>
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<author><name sortKey="Ruiz Diez, Beatriz" sort="Ruiz Diez, Beatriz" uniqKey="Ruiz Diez B" first="Beatriz" last="Ruiz-Díez">Beatriz Ruiz-Díez</name>
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<author><name sortKey="Garcia Fraile, Sonia" sort="Garcia Fraile, Sonia" uniqKey="Garcia Fraile S" first="Sonia" last="García-Fraile">Sonia García-Fraile</name>
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<author><name sortKey="Garcia, Jose Antonio Lucas" sort="Garcia, Jose Antonio Lucas" uniqKey="Garcia J" first="José Antonio Lucas" last="García">José Antonio Lucas García</name>
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<author><name sortKey="Fernandez Pascual, Mercedes" sort="Fernandez Pascual, Mercedes" uniqKey="Fernandez Pascual M" first="Mercedes" last="Fernández-Pascual">Mercedes Fernández-Pascual</name>
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<term>Bacterial Proteins (metabolism)</term>
<term>Basidiomycota (growth & development)</term>
<term>Colony Count, Microbial (MeSH)</term>
<term>Green Fluorescent Proteins (genetics)</term>
<term>Green Fluorescent Proteins (metabolism)</term>
<term>Mycorrhizae (MeSH)</term>
<term>Pinus (growth & development)</term>
<term>Pinus (microbiology)</term>
<term>Plant Roots (microbiology)</term>
<term>Pseudomonas fluorescens (genetics)</term>
<term>Pseudomonas fluorescens (growth & development)</term>
<term>Pseudomonas fluorescens (metabolism)</term>
<term>Soil Microbiology (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term>
<term>Green Fluorescent Proteins</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pseudomonas fluorescens</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Basidiomycota</term>
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<front><div type="abstract" xml:lang="en">Colonisation of Pinus halepensis roots by GFP-tagged Pseudomonas fluorescens Aur6 was monitored by epifluorescence microscopy and dilution plating. Aur6-GFP was able to colonise and proliferate on P. halepensis roots. Co-inoculation with the ectomycorrhizal fungus Suillus granulatus did not affect the bacterial colonisation pattern whereas it had an effect on bacterial density. Bacterial counts increased during the first 20 days of seedling growth, irrespective of seedlings being mycorrhizal or not. After 40 days, bacterial density significantly decreased and bacteria concentrated on the upper two-thirds of the pine root. The presence of S. granulatus significantly stimulated survival of bacteria in the root elongation zone where fungal colonisation was higher. The number of mycorrhizas formed by S. granulatus was not affected by co-inoculation with Aur6-GFP. Neither Aur6-GFP nor S. granulatus stimulated P. halepensis development when inoculated alone, but a synergistic effect was observed on seedling growth when bacteria and fungus were co-inoculated.</div>
</front>
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<Abstract><AbstractText>Colonisation of Pinus halepensis roots by GFP-tagged Pseudomonas fluorescens Aur6 was monitored by epifluorescence microscopy and dilution plating. Aur6-GFP was able to colonise and proliferate on P. halepensis roots. Co-inoculation with the ectomycorrhizal fungus Suillus granulatus did not affect the bacterial colonisation pattern whereas it had an effect on bacterial density. Bacterial counts increased during the first 20 days of seedling growth, irrespective of seedlings being mycorrhizal or not. After 40 days, bacterial density significantly decreased and bacteria concentrated on the upper two-thirds of the pine root. The presence of S. granulatus significantly stimulated survival of bacteria in the root elongation zone where fungal colonisation was higher. The number of mycorrhizas formed by S. granulatus was not affected by co-inoculation with Aur6-GFP. Neither Aur6-GFP nor S. granulatus stimulated P. halepensis development when inoculated alone, but a synergistic effect was observed on seedling growth when bacteria and fungus were co-inoculated.</AbstractText>
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<ForeName>Ana</ForeName>
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