The auxin-inducible GH3 homologue Pp-GH3.16 is downregulated in Pinus pinaster root systems on ectomycorrhizal symbiosis establishment.
Identifieur interne : 003300 ( Main/Corpus ); précédent : 003299; suivant : 003301The auxin-inducible GH3 homologue Pp-GH3.16 is downregulated in Pinus pinaster root systems on ectomycorrhizal symbiosis establishment.
Auteurs : S M Reddy ; S. Hitchin ; D. Melayah ; A K Pandey ; C. Raffier ; J. Henderson ; R. Marmeisse ; G. GaySource :
- The New phytologist [ 0028-646X ] ; 2006.
English descriptors
- KwdEn :
- Agaricales (physiology), Amino Acid Sequence (MeSH), Basidiomycota (physiology), Cloning, Molecular (MeSH), Cycloheximide (pharmacology), Down-Regulation (MeSH), Gene Library (MeSH), Indoleacetic Acids (pharmacology), Molecular Sequence Data (MeSH), Mycorrhizae (physiology), Pinus (genetics), Pinus (metabolism), Pinus (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Roots (genetics), Plant Roots (metabolism), Plant Roots (microbiology), RNA, Messenger (metabolism), Sequence Alignment (MeSH), Symbiosis (genetics), Up-Regulation (MeSH).
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Plant Proteins, RNA, Messenger.
- chemical , pharmacology : Cycloheximide, Indoleacetic Acids.
- genetics : Pinus, Plant Roots, Symbiosis.
- metabolism : Pinus, Plant Roots.
- microbiology : Pinus, Plant Roots.
- physiology : Agaricales, Basidiomycota, Mycorrhizae.
- Amino Acid Sequence, Cloning, Molecular, Down-Regulation, Gene Library, Molecular Sequence Data, Sequence Alignment, Up-Regulation.
Abstract
In an attempt to determine whether auxin-regulated plant genes play a role in ectomycorrhizal symbiosis establishment, we screened a Pinus pinaster root cDNA library for auxin-upregulated genes. This allowed the identification of a cDNA, Pp-GH3.16, which encodes a polypeptide sharing extensive homologies with GH3 proteins of different plants. Pp-GH3.16 was specifically upregulated by auxins and was not affected by cytokinin, gibberellin, abscisic acid or ethylene, or by heat shock, water stress or anoxia. Pp-GH3.16 mRNAs were quantified in pine roots inoculated with two ectomycorrhizal fungi, Hebeloma cylindrosporum and Rhizopogon roseolus. Surprisingly, Pp-GH3.16 was downregulated following inoculation with both fungal species. The downregulation was most rapid on establishment of symbiosis with an indole-3-acetic acid (IAA)-overproducing mutant of H. cylindrosporum, which overproduced mycorrhizas characterized by a hypertrophic Hartig net. This indicates that, despite being auxin-inducible, Pp-GH3.16 can be downregulated on establishment of symbiosis with a fungus that releases auxin. By contrast, Pp-GH3.16 was not downregulated in pine root systems inoculated with a nonmycorrhizal mutant of H. cylindrosporum, suggesting that the downregulation we observed in mycorrhizal root systems was a component of the molecular cross-talk between symbiotic partners at the origin of differentiation of symbiotic structures.
DOI: 10.1111/j.1469-8137.2006.01677.x
PubMed: 16608463
Links to Exploration step
pubmed:16608463Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The auxin-inducible GH3 homologue Pp-GH3.16 is downregulated in Pinus pinaster root systems on ectomycorrhizal symbiosis establishment.</title><author><name sortKey="Reddy, S M" sort="Reddy, S M" uniqKey="Reddy S" first="S M" last="Reddy">S M Reddy</name><affiliation><nlm:affiliation>Université Lyon 1, UMR CNRS 5557, USC INRA 1193 d'Ecologie Microbienne Bât. A. Lwoff, 43 boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Hitchin, S" sort="Hitchin, S" uniqKey="Hitchin S" first="S" last="Hitchin">S. Hitchin</name></author><author><name sortKey="Melayah, D" sort="Melayah, D" uniqKey="Melayah D" first="D" last="Melayah">D. Melayah</name></author><author><name sortKey="Pandey, A K" sort="Pandey, A K" uniqKey="Pandey A" first="A K" last="Pandey">A K Pandey</name></author><author><name sortKey="Raffier, C" sort="Raffier, C" uniqKey="Raffier C" first="C" last="Raffier">C. Raffier</name></author><author><name sortKey="Henderson, J" sort="Henderson, J" uniqKey="Henderson J" first="J" last="Henderson">J. Henderson</name></author><author><name sortKey="Marmeisse, R" sort="Marmeisse, R" uniqKey="Marmeisse R" first="R" last="Marmeisse">R. Marmeisse</name></author><author><name sortKey="Gay, G" sort="Gay, G" uniqKey="Gay G" first="G" last="Gay">G. Gay</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16608463</idno><idno type="pmid">16608463</idno><idno type="doi">10.1111/j.1469-8137.2006.01677.x</idno><idno type="wicri:Area/Main/Corpus">003300</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003300</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The auxin-inducible GH3 homologue Pp-GH3.16 is downregulated in Pinus pinaster root systems on ectomycorrhizal symbiosis establishment.</title><author><name sortKey="Reddy, S M" sort="Reddy, S M" uniqKey="Reddy S" first="S M" last="Reddy">S M Reddy</name><affiliation><nlm:affiliation>Université Lyon 1, UMR CNRS 5557, USC INRA 1193 d'Ecologie Microbienne Bât. A. Lwoff, 43 boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Hitchin, S" sort="Hitchin, S" uniqKey="Hitchin S" first="S" last="Hitchin">S. Hitchin</name></author><author><name sortKey="Melayah, D" sort="Melayah, D" uniqKey="Melayah D" first="D" last="Melayah">D. Melayah</name></author><author><name sortKey="Pandey, A K" sort="Pandey, A K" uniqKey="Pandey A" first="A K" last="Pandey">A K Pandey</name></author><author><name sortKey="Raffier, C" sort="Raffier, C" uniqKey="Raffier C" first="C" last="Raffier">C. Raffier</name></author><author><name sortKey="Henderson, J" sort="Henderson, J" uniqKey="Henderson J" first="J" last="Henderson">J. Henderson</name></author><author><name sortKey="Marmeisse, R" sort="Marmeisse, R" uniqKey="Marmeisse R" first="R" last="Marmeisse">R. Marmeisse</name></author><author><name sortKey="Gay, G" sort="Gay, G" uniqKey="Gay G" first="G" last="Gay">G. Gay</name></author></analytic><series><title level="j">The New phytologist</title><idno type="ISSN">0028-646X</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agaricales (physiology)</term><term>Amino Acid Sequence (MeSH)</term><term>Basidiomycota (physiology)</term><term>Cloning, Molecular (MeSH)</term><term>Cycloheximide (pharmacology)</term><term>Down-Regulation (MeSH)</term><term>Gene Library (MeSH)</term><term>Indoleacetic Acids (pharmacology)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (physiology)</term><term>Pinus (genetics)</term><term>Pinus (metabolism)</term><term>Pinus (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant Roots (genetics)</term><term>Plant Roots (metabolism)</term><term>Plant Roots (microbiology)</term><term>RNA, Messenger (metabolism)</term><term>Sequence Alignment (MeSH)</term><term>Symbiosis (genetics)</term><term>Up-Regulation (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cycloheximide</term><term>Indoleacetic Acids</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pinus</term><term>Plant Roots</term><term>Symbiosis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Pinus</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Pinus</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Agaricales</term><term>Basidiomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Cloning, Molecular</term><term>Down-Regulation</term><term>Gene Library</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term><term>Up-Regulation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In an attempt to determine whether auxin-regulated plant genes play a role in ectomycorrhizal symbiosis establishment, we screened a Pinus pinaster root cDNA library for auxin-upregulated genes. This allowed the identification of a cDNA, Pp-GH3.16, which encodes a polypeptide sharing extensive homologies with GH3 proteins of different plants. Pp-GH3.16 was specifically upregulated by auxins and was not affected by cytokinin, gibberellin, abscisic acid or ethylene, or by heat shock, water stress or anoxia. Pp-GH3.16 mRNAs were quantified in pine roots inoculated with two ectomycorrhizal fungi, Hebeloma cylindrosporum and Rhizopogon roseolus. Surprisingly, Pp-GH3.16 was downregulated following inoculation with both fungal species. The downregulation was most rapid on establishment of symbiosis with an indole-3-acetic acid (IAA)-overproducing mutant of H. cylindrosporum, which overproduced mycorrhizas characterized by a hypertrophic Hartig net. This indicates that, despite being auxin-inducible, Pp-GH3.16 can be downregulated on establishment of symbiosis with a fungus that releases auxin. By contrast, Pp-GH3.16 was not downregulated in pine root systems inoculated with a nonmycorrhizal mutant of H. cylindrosporum, suggesting that the downregulation we observed in mycorrhizal root systems was a component of the molecular cross-talk between symbiotic partners at the origin of differentiation of symbiotic structures.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16608463</PMID><DateCompleted><Year>2006</Year><Month>06</Month><Day>05</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0028-646X</ISSN><JournalIssue CitedMedium="Print"><Volume>170</Volume><Issue>2</Issue><PubDate><Year>2006</Year></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>The auxin-inducible GH3 homologue Pp-GH3.16 is downregulated in Pinus pinaster root systems on ectomycorrhizal symbiosis establishment.</ArticleTitle><Pagination><MedlinePgn>391-400</MedlinePgn></Pagination><Abstract><AbstractText>In an attempt to determine whether auxin-regulated plant genes play a role in ectomycorrhizal symbiosis establishment, we screened a Pinus pinaster root cDNA library for auxin-upregulated genes. This allowed the identification of a cDNA, Pp-GH3.16, which encodes a polypeptide sharing extensive homologies with GH3 proteins of different plants. Pp-GH3.16 was specifically upregulated by auxins and was not affected by cytokinin, gibberellin, abscisic acid or ethylene, or by heat shock, water stress or anoxia. Pp-GH3.16 mRNAs were quantified in pine roots inoculated with two ectomycorrhizal fungi, Hebeloma cylindrosporum and Rhizopogon roseolus. Surprisingly, Pp-GH3.16 was downregulated following inoculation with both fungal species. The downregulation was most rapid on establishment of symbiosis with an indole-3-acetic acid (IAA)-overproducing mutant of H. cylindrosporum, which overproduced mycorrhizas characterized by a hypertrophic Hartig net. This indicates that, despite being auxin-inducible, Pp-GH3.16 can be downregulated on establishment of symbiosis with a fungus that releases auxin. By contrast, Pp-GH3.16 was not downregulated in pine root systems inoculated with a nonmycorrhizal mutant of H. cylindrosporum, suggesting that the downregulation we observed in mycorrhizal root systems was a component of the molecular cross-talk between symbiotic partners at the origin of differentiation of symbiotic structures.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Reddy</LastName><ForeName>S M</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Université Lyon 1, UMR CNRS 5557, USC INRA 1193 d'Ecologie Microbienne Bât. A. 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MajorTopicYN="N">Up-Regulation</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>4</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>6</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>4</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16608463</ArticleId><ArticleId IdType="pii">NPH1677</ArticleId><ArticleId IdType="doi">10.1111/j.1469-8137.2006.01677.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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