MycorrhizaeV1, Main, Corpus, bibRecord, 003234

A novel in vitro cultivation system to produce and isolate soluble factors released from hyphae of arbuscular mycorrhizal fungi.

Identifieur interne : 003234 ( Main/Corpus ); précédent : 003233; suivant : 003235

A novel in vitro cultivation system to produce and isolate soluble factors released from hyphae of arbuscular mycorrhizal fungi.

Auteurs : Vijay Gadkar ; James D. Driver ; Matthias C. Rillig

Source :

Biotechnology letters [ 0141-5492 ] ; 2006.

RBID : pubmed:16802104

English descriptors

KwdEn :
- Bioreactors (MeSH), Cell Culture Techniques (instrumentation), Cell Culture Techniques (methods), Daucus carota (chemistry), Daucus carota (microbiology), Equipment Design (MeSH), Equipment Failure Analysis (MeSH), Fungal Proteins (biosynthesis), Fungal Proteins (chemistry), Fungal Proteins (isolation & purification), Hyphae (metabolism), Mycorrhizae (chemistry), Mycorrhizae (metabolism), Solubility (MeSH).
MESH :
- chemical , biosynthesis : Fungal Proteins.
- chemistry : Daucus carota, Fungal Proteins, Mycorrhizae.
- instrumentation : Cell Culture Techniques.
- chemical , isolation & purification : Fungal Proteins.
- metabolism : Hyphae, Mycorrhizae.
- methods : Cell Culture Techniques.
- microbiology : Daucus carota.
- Bioreactors, Equipment Design, Equipment Failure Analysis, Solubility.

Abstract

The Petri plate dual in vitro culture system for arbuscular mycorrhizal fungi (AMF) is ill-suited for the production and isolation of extraradically released soluble factors, thereby preventing the further characterization of such compounds. To overcome this technical bottleneck, we here describe a novel cultivation system using the standard AMF Glomus intraradices-carrot dual culture as a model. This new system is capable of producing soluble factors in copious amounts without any interference from host roots. The greatest advantages of this culture method include ease of handling and reusability of the culture vessel, thus making it a very cost effective system.

DOI: 10.1007/s10529-006-9055-1
PubMed: 16802104

Links to Exploration step

pubmed:16802104

Le document en format XML

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<author><name sortKey="Gadkar, Vijay" sort="Gadkar, Vijay" uniqKey="Gadkar V" first="Vijay" last="Gadkar">Vijay Gadkar</name>
<affiliation><nlm:affiliation>Division of Biological Sciences, University of Montana, Missoula, MT 59812-0004, USA. vijay.gadkar@mso.umt.edu</nlm:affiliation>
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<author><name sortKey="Driver, James D" sort="Driver, James D" uniqKey="Driver J" first="James D" last="Driver">James D. Driver</name>
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<author><name sortKey="Rillig, Matthias C" sort="Rillig, Matthias C" uniqKey="Rillig M" first="Matthias C" last="Rillig">Matthias C. Rillig</name>
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<affiliation><nlm:affiliation>Division of Biological Sciences, University of Montana, Missoula, MT 59812-0004, USA. vijay.gadkar@mso.umt.edu</nlm:affiliation>
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<author><name sortKey="Driver, James D" sort="Driver, James D" uniqKey="Driver J" first="James D" last="Driver">James D. Driver</name>
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<term>Daucus carota (chemistry)</term>
<term>Daucus carota (microbiology)</term>
<term>Equipment Design (MeSH)</term>
<term>Equipment Failure Analysis (MeSH)</term>
<term>Fungal Proteins (biosynthesis)</term>
<term>Fungal Proteins (chemistry)</term>
<term>Fungal Proteins (isolation & purification)</term>
<term>Hyphae (metabolism)</term>
<term>Mycorrhizae (chemistry)</term>
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<front><div type="abstract" xml:lang="en">The Petri plate dual in vitro culture system for arbuscular mycorrhizal fungi (AMF) is ill-suited for the production and isolation of extraradically released soluble factors, thereby preventing the further characterization of such compounds. To overcome this technical bottleneck, we here describe a novel cultivation system using the standard AMF Glomus intraradices-carrot dual culture as a model. This new system is capable of producing soluble factors in copious amounts without any interference from host roots. The greatest advantages of this culture method include ease of handling and reusability of the culture vessel, thus making it a very cost effective system.</div>
</front>
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A novel in vitro cultivation system to produce and isolate soluble factors released from hyphae of arbuscular mycorrhizal fungi.

A novel in vitro cultivation system to produce and isolate soluble factors released from hyphae of arbuscular mycorrhizal fungi.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki