Genetic diversity of naturally established ectomycorrhizal fungi on Norway spruce seedlings under nursery conditions.
Identifieur interne : 003229 ( Main/Corpus ); précédent : 003228; suivant : 003230Genetic diversity of naturally established ectomycorrhizal fungi on Norway spruce seedlings under nursery conditions.
Auteurs : L K Trocha ; M. Rudawska ; T. Leski ; M. DabertSource :
- Microbial ecology [ 0095-3628 ] ; 2006.
English descriptors
- KwdEn :
- Analysis of Variance (MeSH), Ascomycota (classification), Ascomycota (genetics), Ascomycota (growth & development), Basidiomycota (classification), Basidiomycota (genetics), Basidiomycota (growth & development), DNA, Fungal (chemistry), DNA, Fungal (genetics), DNA, Ribosomal Spacer (chemistry), DNA, Ribosomal Spacer (genetics), Fertilizers (MeSH), Genetic Variation (MeSH), Genotype (MeSH), Molecular Sequence Data (MeSH), Mycorrhizae (classification), Mycorrhizae (genetics), Mycorrhizae (growth & development), Norway (MeSH), Phylogeny (MeSH), Picea (microbiology), Plant Roots (microbiology), Polymorphism, Restriction Fragment Length (MeSH), Restriction Mapping (MeSH), Seedlings (microbiology), Sequence Alignment (MeSH), Sequence Analysis, DNA (MeSH), Soil Microbiology (MeSH).
- MESH :
- chemical , chemistry : DNA, Fungal, DNA, Ribosomal Spacer.
- geographic : Norway.
- classification : Ascomycota, Basidiomycota, Mycorrhizae.
- genetics : Ascomycota, Basidiomycota, DNA, Fungal, DNA, Ribosomal Spacer, Mycorrhizae.
- growth & development : Ascomycota, Basidiomycota, Mycorrhizae.
- microbiology : Picea, Plant Roots, Seedlings.
- Analysis of Variance, Fertilizers, Genetic Variation, Genotype, Molecular Sequence Data, Phylogeny, Polymorphism, Restriction Fragment Length, Restriction Mapping, Sequence Alignment, Sequence Analysis, DNA, Soil Microbiology.
Abstract
We have assessed ectomycorrhizal fungi colonizing Norway spruce (Picea abies L.) seedlings in nine forest nurseries using restriction fragment length polymorphism (RFLP) and sequencing analyses of the internal transcribed spacers (ITS1-5.8S-ITS2) amplicons. Restriction analysis of the amplified DNA fragments with HinfI, MboI, and TaqI enzymes allowed the definition of 17 RFLP genotypes; five of them could be unambiguously assigned to Thelephora terrestris, Hebeloma longicaudum, H. crustuliniforme, Tricharina ochroleuca, and Cenococcum geophilum species by comparison with the sporocarp RFLP-pattern database. The remaining genotypes have been sequenced and compared with sequences deposited in the GenBank database. The phylogenetic analysis of resulting sequences and their identified matches indicated that isolated genotypes have formed seven clades. The ascomycetes were predominant: we have determined eight species--Wilcoxina mikolae, Phialophora finlandia, Tuber sp., Cenococcum geophilum, Tricharina ochroleuca, Pulvinula constellatio, and two unidentified ascomycetes--whereas the basidiomycetes were less common (four species denoted: Amphinema byssoides, Hebeloma crustuliniforme, H. longicaudum, and Thelephora terrestris). Wilcoxina mikolae and Phialophora finlandia were the most frequent fungi. Analysis of variance revealed that ascomycetes abundance was higher in nurseries that used organic fertilizer.
DOI: 10.1007/s00248-006-9110-4
PubMed: 16826321
Links to Exploration step
pubmed:16826321Le document en format XML
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<author><name sortKey="Trocha, L K" sort="Trocha, L K" uniqKey="Trocha L" first="L K" last="Trocha">L K Trocha</name>
<affiliation><nlm:affiliation>Institute of Dendrology, Polish Academy of Sciences, 5 Parkowa, 62-035 Kornik, Poland.</nlm:affiliation>
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<author><name sortKey="Rudawska, M" sort="Rudawska, M" uniqKey="Rudawska M" first="M" last="Rudawska">M. Rudawska</name>
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<author><name sortKey="Leski, T" sort="Leski, T" uniqKey="Leski T" first="T" last="Leski">T. Leski</name>
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<author><name sortKey="Dabert, M" sort="Dabert, M" uniqKey="Dabert M" first="M" last="Dabert">M. Dabert</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analysis of Variance (MeSH)</term>
<term>Ascomycota (classification)</term>
<term>Ascomycota (genetics)</term>
<term>Ascomycota (growth & development)</term>
<term>Basidiomycota (classification)</term>
<term>Basidiomycota (genetics)</term>
<term>Basidiomycota (growth & development)</term>
<term>DNA, Fungal (chemistry)</term>
<term>DNA, Fungal (genetics)</term>
<term>DNA, Ribosomal Spacer (chemistry)</term>
<term>DNA, Ribosomal Spacer (genetics)</term>
<term>Fertilizers (MeSH)</term>
<term>Genetic Variation (MeSH)</term>
<term>Genotype (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Mycorrhizae (classification)</term>
<term>Mycorrhizae (genetics)</term>
<term>Mycorrhizae (growth & development)</term>
<term>Norway (MeSH)</term>
<term>Phylogeny (MeSH)</term>
<term>Picea (microbiology)</term>
<term>Plant Roots (microbiology)</term>
<term>Polymorphism, Restriction Fragment Length (MeSH)</term>
<term>Restriction Mapping (MeSH)</term>
<term>Seedlings (microbiology)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Soil Microbiology (MeSH)</term>
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<term>DNA, Ribosomal Spacer</term>
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<term>Basidiomycota</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Ascomycota</term>
<term>Basidiomycota</term>
<term>Mycorrhizae</term>
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<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Picea</term>
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<term>Seedlings</term>
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<term>Genotype</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Polymorphism, Restriction Fragment Length</term>
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<front><div type="abstract" xml:lang="en">We have assessed ectomycorrhizal fungi colonizing Norway spruce (Picea abies L.) seedlings in nine forest nurseries using restriction fragment length polymorphism (RFLP) and sequencing analyses of the internal transcribed spacers (ITS1-5.8S-ITS2) amplicons. Restriction analysis of the amplified DNA fragments with HinfI, MboI, and TaqI enzymes allowed the definition of 17 RFLP genotypes; five of them could be unambiguously assigned to Thelephora terrestris, Hebeloma longicaudum, H. crustuliniforme, Tricharina ochroleuca, and Cenococcum geophilum species by comparison with the sporocarp RFLP-pattern database. The remaining genotypes have been sequenced and compared with sequences deposited in the GenBank database. The phylogenetic analysis of resulting sequences and their identified matches indicated that isolated genotypes have formed seven clades. The ascomycetes were predominant: we have determined eight species--Wilcoxina mikolae, Phialophora finlandia, Tuber sp., Cenococcum geophilum, Tricharina ochroleuca, Pulvinula constellatio, and two unidentified ascomycetes--whereas the basidiomycetes were less common (four species denoted: Amphinema byssoides, Hebeloma crustuliniforme, H. longicaudum, and Thelephora terrestris). Wilcoxina mikolae and Phialophora finlandia were the most frequent fungi. Analysis of variance revealed that ascomycetes abundance was higher in nurseries that used organic fertilizer.</div>
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<Abstract><AbstractText>We have assessed ectomycorrhizal fungi colonizing Norway spruce (Picea abies L.) seedlings in nine forest nurseries using restriction fragment length polymorphism (RFLP) and sequencing analyses of the internal transcribed spacers (ITS1-5.8S-ITS2) amplicons. Restriction analysis of the amplified DNA fragments with HinfI, MboI, and TaqI enzymes allowed the definition of 17 RFLP genotypes; five of them could be unambiguously assigned to Thelephora terrestris, Hebeloma longicaudum, H. crustuliniforme, Tricharina ochroleuca, and Cenococcum geophilum species by comparison with the sporocarp RFLP-pattern database. The remaining genotypes have been sequenced and compared with sequences deposited in the GenBank database. The phylogenetic analysis of resulting sequences and their identified matches indicated that isolated genotypes have formed seven clades. The ascomycetes were predominant: we have determined eight species--Wilcoxina mikolae, Phialophora finlandia, Tuber sp., Cenococcum geophilum, Tricharina ochroleuca, Pulvinula constellatio, and two unidentified ascomycetes--whereas the basidiomycetes were less common (four species denoted: Amphinema byssoides, Hebeloma crustuliniforme, H. longicaudum, and Thelephora terrestris). Wilcoxina mikolae and Phialophora finlandia were the most frequent fungi. Analysis of variance revealed that ascomycetes abundance was higher in nurseries that used organic fertilizer.</AbstractText>
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