Genetic diversity of naturally established ectomycorrhizal fungi on Norway spruce seedlings under nursery conditions.
Identifieur interne : 003229 ( Main/Corpus ); précédent : 003228; suivant : 003230Genetic diversity of naturally established ectomycorrhizal fungi on Norway spruce seedlings under nursery conditions.
Auteurs : L K Trocha ; M. Rudawska ; T. Leski ; M. DabertSource :
- Microbial ecology [ 0095-3628 ] ; 2006.
English descriptors
- KwdEn :
- Analysis of Variance (MeSH), Ascomycota (classification), Ascomycota (genetics), Ascomycota (growth & development), Basidiomycota (classification), Basidiomycota (genetics), Basidiomycota (growth & development), DNA, Fungal (chemistry), DNA, Fungal (genetics), DNA, Ribosomal Spacer (chemistry), DNA, Ribosomal Spacer (genetics), Fertilizers (MeSH), Genetic Variation (MeSH), Genotype (MeSH), Molecular Sequence Data (MeSH), Mycorrhizae (classification), Mycorrhizae (genetics), Mycorrhizae (growth & development), Norway (MeSH), Phylogeny (MeSH), Picea (microbiology), Plant Roots (microbiology), Polymorphism, Restriction Fragment Length (MeSH), Restriction Mapping (MeSH), Seedlings (microbiology), Sequence Alignment (MeSH), Sequence Analysis, DNA (MeSH), Soil Microbiology (MeSH).
- MESH :
- chemical , chemistry : DNA, Fungal, DNA, Ribosomal Spacer.
- geographic : Norway.
- classification : Ascomycota, Basidiomycota, Mycorrhizae.
- genetics : Ascomycota, Basidiomycota, DNA, Fungal, DNA, Ribosomal Spacer, Mycorrhizae.
- growth & development : Ascomycota, Basidiomycota, Mycorrhizae.
- microbiology : Picea, Plant Roots, Seedlings.
- Analysis of Variance, Fertilizers, Genetic Variation, Genotype, Molecular Sequence Data, Phylogeny, Polymorphism, Restriction Fragment Length, Restriction Mapping, Sequence Alignment, Sequence Analysis, DNA, Soil Microbiology.
Abstract
We have assessed ectomycorrhizal fungi colonizing Norway spruce (Picea abies L.) seedlings in nine forest nurseries using restriction fragment length polymorphism (RFLP) and sequencing analyses of the internal transcribed spacers (ITS1-5.8S-ITS2) amplicons. Restriction analysis of the amplified DNA fragments with HinfI, MboI, and TaqI enzymes allowed the definition of 17 RFLP genotypes; five of them could be unambiguously assigned to Thelephora terrestris, Hebeloma longicaudum, H. crustuliniforme, Tricharina ochroleuca, and Cenococcum geophilum species by comparison with the sporocarp RFLP-pattern database. The remaining genotypes have been sequenced and compared with sequences deposited in the GenBank database. The phylogenetic analysis of resulting sequences and their identified matches indicated that isolated genotypes have formed seven clades. The ascomycetes were predominant: we have determined eight species--Wilcoxina mikolae, Phialophora finlandia, Tuber sp., Cenococcum geophilum, Tricharina ochroleuca, Pulvinula constellatio, and two unidentified ascomycetes--whereas the basidiomycetes were less common (four species denoted: Amphinema byssoides, Hebeloma crustuliniforme, H. longicaudum, and Thelephora terrestris). Wilcoxina mikolae and Phialophora finlandia were the most frequent fungi. Analysis of variance revealed that ascomycetes abundance was higher in nurseries that used organic fertilizer.
DOI: 10.1007/s00248-006-9110-4
PubMed: 16826321
Links to Exploration step
pubmed:16826321Le document en format XML
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Dabert</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16826321</idno><idno type="pmid">16826321</idno><idno type="doi">10.1007/s00248-006-9110-4</idno><idno type="wicri:Area/Main/Corpus">003229</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003229</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genetic diversity of naturally established ectomycorrhizal fungi on Norway spruce seedlings under nursery conditions.</title><author><name sortKey="Trocha, L K" sort="Trocha, L K" uniqKey="Trocha L" first="L K" last="Trocha">L K Trocha</name><affiliation><nlm:affiliation>Institute of Dendrology, Polish Academy of Sciences, 5 Parkowa, 62-035 Kornik, Poland.</nlm:affiliation></affiliation></author><author><name sortKey="Rudawska, M" sort="Rudawska, M" uniqKey="Rudawska M" first="M" last="Rudawska">M. 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Dabert</name></author></analytic><series><title level="j">Microbial ecology</title><idno type="ISSN">0095-3628</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analysis of Variance (MeSH)</term><term>Ascomycota (classification)</term><term>Ascomycota (genetics)</term><term>Ascomycota (growth & development)</term><term>Basidiomycota (classification)</term><term>Basidiomycota (genetics)</term><term>Basidiomycota (growth & development)</term><term>DNA, Fungal (chemistry)</term><term>DNA, Fungal (genetics)</term><term>DNA, Ribosomal Spacer (chemistry)</term><term>DNA, Ribosomal Spacer (genetics)</term><term>Fertilizers (MeSH)</term><term>Genetic Variation (MeSH)</term><term>Genotype (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (classification)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (growth & development)</term><term>Norway (MeSH)</term><term>Phylogeny (MeSH)</term><term>Picea (microbiology)</term><term>Plant Roots (microbiology)</term><term>Polymorphism, Restriction Fragment Length (MeSH)</term><term>Restriction Mapping (MeSH)</term><term>Seedlings (microbiology)</term><term>Sequence Alignment (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Soil Microbiology (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Fungal</term><term>DNA, Ribosomal Spacer</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Norway</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Ascomycota</term><term>Basidiomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ascomycota</term><term>Basidiomycota</term><term>DNA, Fungal</term><term>DNA, Ribosomal Spacer</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Ascomycota</term><term>Basidiomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Picea</term><term>Plant Roots</term><term>Seedlings</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Analysis of Variance</term><term>Fertilizers</term><term>Genetic Variation</term><term>Genotype</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Polymorphism, Restriction Fragment Length</term><term>Restriction Mapping</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Soil Microbiology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have assessed ectomycorrhizal fungi colonizing Norway spruce (Picea abies L.) seedlings in nine forest nurseries using restriction fragment length polymorphism (RFLP) and sequencing analyses of the internal transcribed spacers (ITS1-5.8S-ITS2) amplicons. Restriction analysis of the amplified DNA fragments with HinfI, MboI, and TaqI enzymes allowed the definition of 17 RFLP genotypes; five of them could be unambiguously assigned to Thelephora terrestris, Hebeloma longicaudum, H. crustuliniforme, Tricharina ochroleuca, and Cenococcum geophilum species by comparison with the sporocarp RFLP-pattern database. The remaining genotypes have been sequenced and compared with sequences deposited in the GenBank database. The phylogenetic analysis of resulting sequences and their identified matches indicated that isolated genotypes have formed seven clades. The ascomycetes were predominant: we have determined eight species--Wilcoxina mikolae, Phialophora finlandia, Tuber sp., Cenococcum geophilum, Tricharina ochroleuca, Pulvinula constellatio, and two unidentified ascomycetes--whereas the basidiomycetes were less common (four species denoted: Amphinema byssoides, Hebeloma crustuliniforme, H. longicaudum, and Thelephora terrestris). Wilcoxina mikolae and Phialophora finlandia were the most frequent fungi. Analysis of variance revealed that ascomycetes abundance was higher in nurseries that used organic fertilizer.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16826321</PMID><DateCompleted><Year>2007</Year><Month>10</Month><Day>25</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0095-3628</ISSN><JournalIssue CitedMedium="Print"><Volume>52</Volume><Issue>3</Issue><PubDate><Year>2006</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Microbial ecology</Title><ISOAbbreviation>Microb Ecol</ISOAbbreviation></Journal><ArticleTitle>Genetic diversity of naturally established ectomycorrhizal fungi on Norway spruce seedlings under nursery conditions.</ArticleTitle><Pagination><MedlinePgn>418-25</MedlinePgn></Pagination><Abstract><AbstractText>We have assessed ectomycorrhizal fungi colonizing Norway spruce (Picea abies L.) seedlings in nine forest nurseries using restriction fragment length polymorphism (RFLP) and sequencing analyses of the internal transcribed spacers (ITS1-5.8S-ITS2) amplicons. Restriction analysis of the amplified DNA fragments with HinfI, MboI, and TaqI enzymes allowed the definition of 17 RFLP genotypes; five of them could be unambiguously assigned to Thelephora terrestris, Hebeloma longicaudum, H. crustuliniforme, Tricharina ochroleuca, and Cenococcum geophilum species by comparison with the sporocarp RFLP-pattern database. The remaining genotypes have been sequenced and compared with sequences deposited in the GenBank database. The phylogenetic analysis of resulting sequences and their identified matches indicated that isolated genotypes have formed seven clades. The ascomycetes were predominant: we have determined eight species--Wilcoxina mikolae, Phialophora finlandia, Tuber sp., Cenococcum geophilum, Tricharina ochroleuca, Pulvinula constellatio, and two unidentified ascomycetes--whereas the basidiomycetes were less common (four species denoted: Amphinema byssoides, Hebeloma crustuliniforme, H. longicaudum, and Thelephora terrestris). Wilcoxina mikolae and Phialophora finlandia were the most frequent fungi. Analysis of variance revealed that ascomycetes abundance was higher in nurseries that used organic fertilizer.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Trocha</LastName><ForeName>L K</ForeName><Initials>LK</Initials><AffiliationInfo><Affiliation>Institute of Dendrology, Polish Academy of Sciences, 5 Parkowa, 62-035 Kornik, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rudawska</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Leski</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Dabert</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>07</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Microb Ecol</MedlineTA><NlmUniqueID>7500663</NlmUniqueID><ISSNLinking>0095-3628</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021903">DNA, Ribosomal Spacer</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005308">Fertilizers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000704" MajorTopicYN="N">Analysis of Variance</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021903" MajorTopicYN="N">DNA, Ribosomal Spacer</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName 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MajorTopicYN="N">Picea</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012150" MajorTopicYN="N">Polymorphism, Restriction Fragment Length</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015183" MajorTopicYN="N">Restriction Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D036226" MajorTopicYN="N">Seedlings</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="N">Soil Microbiology</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2006</Year><Month>04</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2006</Year><Month>04</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>7</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>10</Month><Day>27</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>7</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16826321</ArticleId><ArticleId IdType="doi">10.1007/s00248-006-9110-4</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Mycologia. 2005 May-Jun;97(3):628-40</Citation><ArticleIdList><ArticleId 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