Mutualistic mycorrhiza in orchids: evidence from plant-fungus carbon and nitrogen transfers in the green-leaved terrestrial orchid Goodyera repens.
Identifieur interne : 003217 ( Main/Corpus ); précédent : 003216; suivant : 003218Mutualistic mycorrhiza in orchids: evidence from plant-fungus carbon and nitrogen transfers in the green-leaved terrestrial orchid Goodyera repens.
Auteurs : Duncan D. Cameron ; Jonathan R. Leake ; David J. ReadSource :
- The New phytologist [ 0028-646X ] ; 2006.
English descriptors
- KwdEn :
- Carbon (metabolism), Carbon Dioxide (metabolism), Carbon Isotopes (metabolism), Glycine (metabolism), Mycorrhizae (metabolism), Mycorrhizae (physiology), Nitrogen (metabolism), Nitrogen Isotopes (metabolism), Orchidaceae (metabolism), Orchidaceae (physiology), Plant Leaves (metabolism), Plant Roots (metabolism), Symbiosis (physiology).
- MESH :
- chemical , metabolism : Carbon, Carbon Dioxide, Carbon Isotopes, Glycine, Nitrogen, Nitrogen Isotopes.
- metabolism : Mycorrhizae, Orchidaceae, Plant Leaves, Plant Roots.
- physiology : Mycorrhizae, Orchidaceae, Symbiosis.
Abstract
The roles of mycorrhiza in facilitating the acquisition and transfer of carbon (C) and nitrogen (N) to adult orchids are poorly understood. Here, we employed isotopically labelled sources of C and N to investigate these processes in the green forest orchid, Goodyera repens. Fungus-to-orchid transfers of C and N were measured using mass spectrometry after supplying extraradical mycelial systems with double-labelled [13C-15N]glycine. Orchid-to-fungus C transfer was revealed and quantified by radioisotope imaging and liquid scintillation counting of extraradical mycelium following 14CO2 fixation by shoots. Both 13C and 15N were assimilated by the fungus and transferred to the roots and shoots of the orchid. Contrary to previous reports, considerable quantities (2.6% over 72 h) of fixed C were shown to be allocated to the extraradical mycelium of the fungus. This study demonstrates, for the first time, mutualism in orchid mycorrhiza, bidirectional transfer of C between a green orchid and its fungal symbiont, and a fungus-dependent pathway for organic N acquisition by an orchid.
DOI: 10.1111/j.1469-8137.2006.01767.x
PubMed: 16866946
Links to Exploration step
pubmed:16866946Le document en format XML
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Read</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16866946</idno><idno type="pmid">16866946</idno><idno type="doi">10.1111/j.1469-8137.2006.01767.x</idno><idno type="wicri:Area/Main/Corpus">003217</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003217</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mutualistic mycorrhiza in orchids: evidence from plant-fungus carbon and nitrogen transfers in the green-leaved terrestrial orchid Goodyera repens.</title><author><name sortKey="Cameron, Duncan D" sort="Cameron, Duncan D" uniqKey="Cameron D" first="Duncan D" last="Cameron">Duncan D. Cameron</name><affiliation><nlm:affiliation>Department of Animal and Plant Sciences, University of Sheffield, Alfred Denny Building, Western Bank, Sheffield S10 2TN, UK. d.cameron@sheffield.ac.uk</nlm:affiliation></affiliation></author><author><name sortKey="Leake, Jonathan R" sort="Leake, Jonathan R" uniqKey="Leake J" first="Jonathan R" last="Leake">Jonathan R. Leake</name></author><author><name sortKey="Read, David J" sort="Read, David J" uniqKey="Read D" first="David J" last="Read">David J. 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Here, we employed isotopically labelled sources of C and N to investigate these processes in the green forest orchid, Goodyera repens. Fungus-to-orchid transfers of C and N were measured using mass spectrometry after supplying extraradical mycelial systems with double-labelled [13C-15N]glycine. Orchid-to-fungus C transfer was revealed and quantified by radioisotope imaging and liquid scintillation counting of extraradical mycelium following 14CO2 fixation by shoots. Both 13C and 15N were assimilated by the fungus and transferred to the roots and shoots of the orchid. Contrary to previous reports, considerable quantities (2.6% over 72 h) of fixed C were shown to be allocated to the extraradical mycelium of the fungus. This study demonstrates, for the first time, mutualism in orchid mycorrhiza, bidirectional transfer of C between a green orchid and its fungal symbiont, and a fungus-dependent pathway for organic N acquisition by an orchid.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16866946</PMID><DateCompleted><Year>2006</Year><Month>09</Month><Day>14</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0028-646X</ISSN><JournalIssue CitedMedium="Print"><Volume>171</Volume><Issue>2</Issue><PubDate><Year>2006</Year></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Mutualistic mycorrhiza in orchids: evidence from plant-fungus carbon and nitrogen transfers in the green-leaved terrestrial orchid Goodyera repens.</ArticleTitle><Pagination><MedlinePgn>405-16</MedlinePgn></Pagination><Abstract><AbstractText>The roles of mycorrhiza in facilitating the acquisition and transfer of carbon (C) and nitrogen (N) to adult orchids are poorly understood. Here, we employed isotopically labelled sources of C and N to investigate these processes in the green forest orchid, Goodyera repens. Fungus-to-orchid transfers of C and N were measured using mass spectrometry after supplying extraradical mycelial systems with double-labelled [13C-15N]glycine. Orchid-to-fungus C transfer was revealed and quantified by radioisotope imaging and liquid scintillation counting of extraradical mycelium following 14CO2 fixation by shoots. Both 13C and 15N were assimilated by the fungus and transferred to the roots and shoots of the orchid. Contrary to previous reports, considerable quantities (2.6% over 72 h) of fixed C were shown to be allocated to the extraradical mycelium of the fungus. This study demonstrates, for the first time, mutualism in orchid mycorrhiza, bidirectional transfer of C between a green orchid and its fungal symbiont, and a fungus-dependent pathway for organic N acquisition by an orchid.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cameron</LastName><ForeName>Duncan D</ForeName><Initials>DD</Initials><AffiliationInfo><Affiliation>Department of Animal and Plant Sciences, University of Sheffield, Alfred Denny Building, Western Bank, Sheffield S10 2TN, UK. d.cameron@sheffield.ac.uk</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leake</LastName><ForeName>Jonathan R</ForeName><Initials>JR</Initials></Author><Author ValidYN="Y"><LastName>Read</LastName><ForeName>David J</ForeName><Initials>DJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002247">Carbon Isotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009587">Nitrogen Isotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>142M471B3J</RegistryNumber><NameOfSubstance UI="D002245">Carbon Dioxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical><Chemical><RegistryNumber>TE7660XO1C</RegistryNumber><NameOfSubstance UI="D005998">Glycine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002245" MajorTopicYN="N">Carbon Dioxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002247" MajorTopicYN="N">Carbon Isotopes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005998" MajorTopicYN="N">Glycine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009587" MajorTopicYN="N">Nitrogen Isotopes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029595" MajorTopicYN="N">Orchidaceae</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>7</Month><Day>27</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>9</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>7</Month><Day>27</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16866946</ArticleId><ArticleId IdType="pii">NPH1767</ArticleId><ArticleId IdType="doi">10.1111/j.1469-8137.2006.01767.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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