MycorrhizaeV1, Main, Corpus, bibRecord, 003167

Medicago truncatula transformation using root explants.

Identifieur interne : 003167 ( Main/Corpus ); précédent : 003166; suivant : 003168

Medicago truncatula transformation using root explants.

Auteurs : Cynthia Crane ; Richard A. Dixon ; Zeng-Yu Wang

Source :

Methods in molecular biology (Clifton, N.J.) [ 1064-3745 ] ; 2006.

RBID : pubmed:16988340

English descriptors

KwdEn :
- Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (growth & development), Gene Transfer Techniques (MeSH), Medicago truncatula (genetics), Medicago truncatula (growth & development), Medicago truncatula (microbiology), Mycorrhizae (genetics), Mycorrhizae (growth & development), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (microbiology), Transformation, Genetic (MeSH).
MESH :
- genetics : Agrobacterium tumefaciens, Medicago truncatula, Mycorrhizae, Plants, Genetically Modified.
- growth & development : Agrobacterium tumefaciens, Medicago truncatula, Mycorrhizae, Plants, Genetically Modified.
- microbiology : Medicago truncatula, Plants, Genetically Modified.
- Gene Transfer Techniques, Transformation, Genetic.

Abstract

Medicago truncatula is a forage crop that has been developed into a model legume. As a model plant, M. truncatula is particularly useful for the study of root endosymbiotic associations, including nodulation and mycorrhizal colonization. The development of different transformation methods is an important aspect for functional genomic studies in the species. This protocol describes an efficient system for generating transgenic plants from M. truncatula roots based on Agrobacterium tumefaciens-mediated transformation. Furthermore, the protocol can be easily adapted for recovering fertile transgenic plants from transformed hairy roots.

DOI: 10.1385/1-59745-130-4:137
PubMed: 16988340

Links to Exploration step

pubmed:16988340

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Medicago truncatula transformation using root explants.</title>
<author><name sortKey="Crane, Cynthia" sort="Crane, Cynthia" uniqKey="Crane C" first="Cynthia" last="Crane">Cynthia Crane</name>
<affiliation><nlm:affiliation>Forage Improvement Division, The Samuel Roberts Noble Foundation, Ardmore, OK, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Dixon, Richard A" sort="Dixon, Richard A" uniqKey="Dixon R" first="Richard A" last="Dixon">Richard A. Dixon</name>
</author>
<author><name sortKey="Wang, Zeng Yu" sort="Wang, Zeng Yu" uniqKey="Wang Z" first="Zeng-Yu" last="Wang">Zeng-Yu Wang</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2006">2006</date>
<idno type="RBID">pubmed:16988340</idno>
<idno type="pmid">16988340</idno>
<idno type="doi">10.1385/1-59745-130-4:137</idno>
<idno type="wicri:Area/Main/Corpus">003167</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003167</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Medicago truncatula transformation using root explants.</title>
<author><name sortKey="Crane, Cynthia" sort="Crane, Cynthia" uniqKey="Crane C" first="Cynthia" last="Crane">Cynthia Crane</name>
<affiliation><nlm:affiliation>Forage Improvement Division, The Samuel Roberts Noble Foundation, Ardmore, OK, USA.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Dixon, Richard A" sort="Dixon, Richard A" uniqKey="Dixon R" first="Richard A" last="Dixon">Richard A. Dixon</name>
</author>
<author><name sortKey="Wang, Zeng Yu" sort="Wang, Zeng Yu" uniqKey="Wang Z" first="Zeng-Yu" last="Wang">Zeng-Yu Wang</name>
</author>
</analytic>
<series><title level="j">Methods in molecular biology (Clifton, N.J.)</title>
<idno type="ISSN">1064-3745</idno>
<imprint><date when="2006" type="published">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term>
<term>Agrobacterium tumefaciens (growth & development)</term>
<term>Gene Transfer Techniques (MeSH)</term>
<term>Medicago truncatula (genetics)</term>
<term>Medicago truncatula (growth & development)</term>
<term>Medicago truncatula (microbiology)</term>
<term>Mycorrhizae (genetics)</term>
<term>Mycorrhizae (growth & development)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (growth & development)</term>
<term>Plants, Genetically Modified (microbiology)</term>
<term>Transformation, Genetic (MeSH)</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Medicago truncatula</term>
<term>Mycorrhizae</term>
<term>Plants, Genetically Modified</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Medicago truncatula</term>
<term>Mycorrhizae</term>
<term>Plants, Genetically Modified</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Medicago truncatula</term>
<term>Plants, Genetically Modified</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Transfer Techniques</term>
<term>Transformation, Genetic</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Medicago truncatula is a forage crop that has been developed into a model legume. As a model plant, M. truncatula is particularly useful for the study of root endosymbiotic associations, including nodulation and mycorrhizal colonization. The development of different transformation methods is an important aspect for functional genomic studies in the species. This protocol describes an efficient system for generating transgenic plants from M. truncatula roots based on Agrobacterium tumefaciens-mediated transformation. Furthermore, the protocol can be easily adapted for recovering fertile transgenic plants from transformed hairy roots.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16988340</PMID>
<DateCompleted><Year>2006</Year>
<Month>10</Month>
<Day>11</Day>
</DateCompleted>
<DateRevised><Year>2011</Year>
<Month>11</Month>
<Day>17</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1064-3745</ISSN>
<JournalIssue CitedMedium="Print"><Volume>343</Volume>
<PubDate><Year>2006</Year>
</PubDate>
</JournalIssue>
<Title>Methods in molecular biology (Clifton, N.J.)</Title>
<ISOAbbreviation>Methods Mol Biol</ISOAbbreviation>
</Journal>
<ArticleTitle>Medicago truncatula transformation using root explants.</ArticleTitle>
<Pagination><MedlinePgn>137-42</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Medicago truncatula is a forage crop that has been developed into a model legume. As a model plant, M. truncatula is particularly useful for the study of root endosymbiotic associations, including nodulation and mycorrhizal colonization. The development of different transformation methods is an important aspect for functional genomic studies in the species. This protocol describes an efficient system for generating transgenic plants from M. truncatula roots based on Agrobacterium tumefaciens-mediated transformation. Furthermore, the protocol can be easily adapted for recovering fertile transgenic plants from transformed hairy roots.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Crane</LastName>
<ForeName>Cynthia</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Forage Improvement Division, The Samuel Roberts Noble Foundation, Ardmore, OK, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dixon</LastName>
<ForeName>Richard A</ForeName>
<Initials>RA</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Zeng-Yu</ForeName>
<Initials>ZY</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D016454">Review</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Methods Mol Biol</MedlineTA>
<NlmUniqueID>9214969</NlmUniqueID>
<ISSNLinking>1064-3745</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018014" MajorTopicYN="Y">Gene Transfer Techniques</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D046913" MajorTopicYN="N">Medicago truncatula</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<NumberOfReferences>5</NumberOfReferences>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year>
<Month>9</Month>
<Day>22</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2006</Year>
<Month>10</Month>
<Day>13</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2006</Year>
<Month>9</Month>
<Day>22</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16988340</ArticleId>
<ArticleId IdType="pii">1-59745-130-4:137</ArticleId>
<ArticleId IdType="doi">10.1385/1-59745-130-4:137</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MycorrhizaeV1/Data/Main/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003167 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 003167 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    MycorrhizaeV1
   |flux=    Main
   |étape=   Corpus
   |type=    RBID
   |clé=     pubmed:16988340
   |texte=   Medicago truncatula transformation using root explants.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i   -Sk "pubmed:16988340" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd   \
       | NlmPubMed2Wicri -a MycorrhizaeV1

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 15:34:48 2020. Site generation: Wed Nov 18 15:41:10 2020

	Serveur d'exploration sur la mycorhize
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur la mycorhize

Medicago truncatula transformation using root explants.

Medicago truncatula transformation using root explants.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki