Diverse tulasnelloid fungi form mycorrhizas with epiphytic orchids in an Andean cloud forest.
Identifieur interne : 003125 ( Main/Corpus ); précédent : 003124; suivant : 003126Diverse tulasnelloid fungi form mycorrhizas with epiphytic orchids in an Andean cloud forest.
Auteurs : Juan Pablo Suárez ; Michael Weiss ; Andrea Abele ; Sigisfredo Garnica ; Franz Oberwinkler ; Ingrid KottkeSource :
- Mycological research [ 0953-7562 ] ; 2006.
English descriptors
- KwdEn :
- Ecuador (MeSH), Microscopy, Electron, Transmission (MeSH), Molecular Sequence Data (MeSH), Mycorrhizae (classification), Mycorrhizae (genetics), Mycorrhizae (ultrastructure), Orchidaceae (microbiology), Phylogeny (MeSH), Plant Roots (microbiology), Plant Roots (ultrastructure), RNA, Ribosomal (genetics), RNA, Ribosomal, 5.8S (genetics), Sequence Analysis, DNA (MeSH), Symbiosis (MeSH).
- MESH :
- chemical , genetics : RNA, Ribosomal, RNA, Ribosomal, 5.8S.
- geographic : Ecuador.
- classification : Mycorrhizae.
- genetics : Mycorrhizae.
- microbiology : Orchidaceae, Plant Roots.
- ultrastructure : Mycorrhizae, Plant Roots.
- Microscopy, Electron, Transmission, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Symbiosis.
Abstract
The mycorrhizal state of epiphytic orchids has been controversially discussed, and the state and mycobionts of the pleurothallid orchids, occurring abundantly and with a high number of species on stems of trees in the Andean cloud forest, were unknown. Root samples of 77 adult individuals of the epiphytic orchids Stelis hallii, S. superbiens, S. concinna and Pleurothallis lilijae were collected in a tropical mountain rainforest of southern Ecuador. Ultrastructural evidence of symbiotic interaction was combined with molecular sequencing of fungi directly from the mycorrhizas and isolation of mycobionts. Ultrastructural analyses displayed vital orchid mycorrhizas formed by fungi with an imperforate parenthesome and cell wall slime bodies typical for the genus Tulasnella. Three different Tulasnella isolates were obtained in pure culture. Phylogenetic analysis of nuclear rDNA sequences from coding regions of the ribosomal large subunit (nucLSU) and the 5.8S subunit, including parts of the internal transcribed spacers, obtained directly from the roots and from the fungal isolates, yielded seven distinct Tulasnella clades. Tulasnella mycobionts in Stelis concinna were restricted to two Tulasnella sequence types while the other orchids were associated with up to six Tulasnella sequence types. All Tulasnella sequences are new to science and distinct from known sequences of mycobionts of terrestrial orchids. The results indicate that tulasnelloid fungi, adapted to the conditions on tree stems, might be important for orchid growth and maintenance in the Andean cloud forest.
DOI: 10.1016/j.mycres.2006.08.004
PubMed: 17081740
Links to Exploration step
pubmed:17081740Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Diverse tulasnelloid fungi form mycorrhizas with epiphytic orchids in an Andean cloud forest.</title><author><name sortKey="Suarez, Juan Pablo" sort="Suarez, Juan Pablo" uniqKey="Suarez J" first="Juan Pablo" last="Suárez">Juan Pablo Suárez</name><affiliation><nlm:affiliation>Centro de Biología Celular y Molecular, Universidad Técnica Particular de Loja, San Cayetano Alto s/n C.P. 11 01 608, Loja, Ecuador. jpsuarez@utpl.edu.ec</nlm:affiliation></affiliation></author><author><name sortKey="Weiss, Michael" sort="Weiss, Michael" uniqKey="Weiss M" first="Michael" last="Weiss">Michael Weiss</name></author><author><name sortKey="Abele, Andrea" sort="Abele, Andrea" uniqKey="Abele A" first="Andrea" last="Abele">Andrea Abele</name></author><author><name sortKey="Garnica, Sigisfredo" sort="Garnica, Sigisfredo" uniqKey="Garnica S" first="Sigisfredo" last="Garnica">Sigisfredo Garnica</name></author><author><name sortKey="Oberwinkler, Franz" sort="Oberwinkler, Franz" uniqKey="Oberwinkler F" first="Franz" last="Oberwinkler">Franz Oberwinkler</name></author><author><name sortKey="Kottke, Ingrid" sort="Kottke, Ingrid" uniqKey="Kottke I" first="Ingrid" last="Kottke">Ingrid Kottke</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:17081740</idno><idno type="pmid">17081740</idno><idno type="doi">10.1016/j.mycres.2006.08.004</idno><idno type="wicri:Area/Main/Corpus">003125</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003125</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Diverse tulasnelloid fungi form mycorrhizas with epiphytic orchids in an Andean cloud forest.</title><author><name sortKey="Suarez, Juan Pablo" sort="Suarez, Juan Pablo" uniqKey="Suarez J" first="Juan Pablo" last="Suárez">Juan Pablo Suárez</name><affiliation><nlm:affiliation>Centro de Biología Celular y Molecular, Universidad Técnica Particular de Loja, San Cayetano Alto s/n C.P. 11 01 608, Loja, Ecuador. jpsuarez@utpl.edu.ec</nlm:affiliation></affiliation></author><author><name sortKey="Weiss, Michael" sort="Weiss, Michael" uniqKey="Weiss M" first="Michael" last="Weiss">Michael Weiss</name></author><author><name sortKey="Abele, Andrea" sort="Abele, Andrea" uniqKey="Abele A" first="Andrea" last="Abele">Andrea Abele</name></author><author><name sortKey="Garnica, Sigisfredo" sort="Garnica, Sigisfredo" uniqKey="Garnica S" first="Sigisfredo" last="Garnica">Sigisfredo Garnica</name></author><author><name sortKey="Oberwinkler, Franz" sort="Oberwinkler, Franz" uniqKey="Oberwinkler F" first="Franz" last="Oberwinkler">Franz Oberwinkler</name></author><author><name sortKey="Kottke, Ingrid" sort="Kottke, Ingrid" uniqKey="Kottke I" first="Ingrid" last="Kottke">Ingrid Kottke</name></author></analytic><series><title level="j">Mycological research</title><idno type="ISSN">0953-7562</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ecuador (MeSH)</term><term>Microscopy, Electron, Transmission (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (classification)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (ultrastructure)</term><term>Orchidaceae (microbiology)</term><term>Phylogeny (MeSH)</term><term>Plant Roots (microbiology)</term><term>Plant Roots (ultrastructure)</term><term>RNA, Ribosomal (genetics)</term><term>RNA, Ribosomal, 5.8S (genetics)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Ribosomal</term><term>RNA, Ribosomal, 5.8S</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Ecuador</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Orchidaceae</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Mycorrhizae</term><term>Plant Roots</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Microscopy, Electron, Transmission</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Symbiosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The mycorrhizal state of epiphytic orchids has been controversially discussed, and the state and mycobionts of the pleurothallid orchids, occurring abundantly and with a high number of species on stems of trees in the Andean cloud forest, were unknown. Root samples of 77 adult individuals of the epiphytic orchids Stelis hallii, S. superbiens, S. concinna and Pleurothallis lilijae were collected in a tropical mountain rainforest of southern Ecuador. Ultrastructural evidence of symbiotic interaction was combined with molecular sequencing of fungi directly from the mycorrhizas and isolation of mycobionts. Ultrastructural analyses displayed vital orchid mycorrhizas formed by fungi with an imperforate parenthesome and cell wall slime bodies typical for the genus Tulasnella. Three different Tulasnella isolates were obtained in pure culture. Phylogenetic analysis of nuclear rDNA sequences from coding regions of the ribosomal large subunit (nucLSU) and the 5.8S subunit, including parts of the internal transcribed spacers, obtained directly from the roots and from the fungal isolates, yielded seven distinct Tulasnella clades. Tulasnella mycobionts in Stelis concinna were restricted to two Tulasnella sequence types while the other orchids were associated with up to six Tulasnella sequence types. All Tulasnella sequences are new to science and distinct from known sequences of mycobionts of terrestrial orchids. The results indicate that tulasnelloid fungi, adapted to the conditions on tree stems, might be important for orchid growth and maintenance in the Andean cloud forest.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17081740</PMID><DateCompleted><Year>2007</Year><Month>01</Month><Day>22</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0953-7562</ISSN><JournalIssue CitedMedium="Print"><Volume>110</Volume><Issue>Pt 11</Issue><PubDate><Year>2006</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Mycological research</Title><ISOAbbreviation>Mycol Res</ISOAbbreviation></Journal><ArticleTitle>Diverse tulasnelloid fungi form mycorrhizas with epiphytic orchids in an Andean cloud forest.</ArticleTitle><Pagination><MedlinePgn>1257-70</MedlinePgn></Pagination><Abstract><AbstractText>The mycorrhizal state of epiphytic orchids has been controversially discussed, and the state and mycobionts of the pleurothallid orchids, occurring abundantly and with a high number of species on stems of trees in the Andean cloud forest, were unknown. Root samples of 77 adult individuals of the epiphytic orchids Stelis hallii, S. superbiens, S. concinna and Pleurothallis lilijae were collected in a tropical mountain rainforest of southern Ecuador. Ultrastructural evidence of symbiotic interaction was combined with molecular sequencing of fungi directly from the mycorrhizas and isolation of mycobionts. Ultrastructural analyses displayed vital orchid mycorrhizas formed by fungi with an imperforate parenthesome and cell wall slime bodies typical for the genus Tulasnella. Three different Tulasnella isolates were obtained in pure culture. Phylogenetic analysis of nuclear rDNA sequences from coding regions of the ribosomal large subunit (nucLSU) and the 5.8S subunit, including parts of the internal transcribed spacers, obtained directly from the roots and from the fungal isolates, yielded seven distinct Tulasnella clades. Tulasnella mycobionts in Stelis concinna were restricted to two Tulasnella sequence types while the other orchids were associated with up to six Tulasnella sequence types. All Tulasnella sequences are new to science and distinct from known sequences of mycobionts of terrestrial orchids. The results indicate that tulasnelloid fungi, adapted to the conditions on tree stems, might be important for orchid growth and maintenance in the Andean cloud forest.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Suárez</LastName><ForeName>Juan Pablo</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>Centro de Biología Celular y Molecular, Universidad Técnica Particular de Loja, San Cayetano Alto s/n C.P. 11 01 608, Loja, Ecuador. jpsuarez@utpl.edu.ec</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Weiss</LastName><ForeName>Michael</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Abele</LastName><ForeName>Andrea</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Garnica</LastName><ForeName>Sigisfredo</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Oberwinkler</LastName><ForeName>Franz</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Kottke</LastName><ForeName>Ingrid</ForeName><Initials>I</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>DQ178029</AccessionNumber><AccessionNumber>DQ178030</AccessionNumber><AccessionNumber>DQ178031</AccessionNumber><AccessionNumber>DQ178032</AccessionNumber><AccessionNumber>DQ178033</AccessionNumber><AccessionNumber>DQ178034</AccessionNumber><AccessionNumber>DQ178035</AccessionNumber><AccessionNumber>DQ178036</AccessionNumber><AccessionNumber>DQ178037</AccessionNumber><AccessionNumber>DQ178038</AccessionNumber><AccessionNumber>DQ178039</AccessionNumber><AccessionNumber>DQ178040</AccessionNumber><AccessionNumber>DQ178041</AccessionNumber><AccessionNumber>DQ178042</AccessionNumber><AccessionNumber>DQ178043</AccessionNumber><AccessionNumber>DQ178044</AccessionNumber><AccessionNumber>DQ178045</AccessionNumber><AccessionNumber>DQ178046</AccessionNumber><AccessionNumber>DQ178047</AccessionNumber><AccessionNumber>DQ178048</AccessionNumber><AccessionNumber>DQ178049</AccessionNumber><AccessionNumber>DQ178050</AccessionNumber><AccessionNumber>DQ178051</AccessionNumber><AccessionNumber>DQ178052</AccessionNumber><AccessionNumber>DQ178053</AccessionNumber><AccessionNumber>DQ178054</AccessionNumber><AccessionNumber>DQ178055</AccessionNumber><AccessionNumber>DQ178056</AccessionNumber><AccessionNumber>DQ178057</AccessionNumber><AccessionNumber>DQ178058</AccessionNumber><AccessionNumber>DQ178059</AccessionNumber><AccessionNumber>DQ178060</AccessionNumber><AccessionNumber>DQ178061</AccessionNumber><AccessionNumber>DQ178062</AccessionNumber><AccessionNumber>DQ178063</AccessionNumber><AccessionNumber>DQ178064</AccessionNumber><AccessionNumber>DQ178065</AccessionNumber><AccessionNumber>DQ178066</AccessionNumber><AccessionNumber>DQ178067</AccessionNumber><AccessionNumber>DQ178068</AccessionNumber><AccessionNumber>DQ178069</AccessionNumber><AccessionNumber>DQ178070</AccessionNumber><AccessionNumber>DQ178071</AccessionNumber><AccessionNumber>DQ178072</AccessionNumber><AccessionNumber>DQ178073</AccessionNumber><AccessionNumber>DQ178074</AccessionNumber><AccessionNumber>DQ178075</AccessionNumber><AccessionNumber>DQ178076</AccessionNumber><AccessionNumber>DQ178077</AccessionNumber><AccessionNumber>DQ178078</AccessionNumber><AccessionNumber>DQ178079</AccessionNumber><AccessionNumber>DQ178080</AccessionNumber><AccessionNumber>DQ178081</AccessionNumber><AccessionNumber>DQ178082</AccessionNumber><AccessionNumber>DQ178083</AccessionNumber><AccessionNumber>DQ178084</AccessionNumber><AccessionNumber>DQ178085</AccessionNumber><AccessionNumber>DQ178086</AccessionNumber><AccessionNumber>DQ178087</AccessionNumber><AccessionNumber>DQ178088</AccessionNumber><AccessionNumber>DQ178089</AccessionNumber><AccessionNumber>DQ178090</AccessionNumber><AccessionNumber>DQ178091</AccessionNumber><AccessionNumber>DQ178092</AccessionNumber><AccessionNumber>DQ178093</AccessionNumber><AccessionNumber>DQ178094</AccessionNumber><AccessionNumber>DQ178095</AccessionNumber><AccessionNumber>DQ178096</AccessionNumber><AccessionNumber>DQ178097</AccessionNumber><AccessionNumber>DQ178098</AccessionNumber><AccessionNumber>DQ178099</AccessionNumber><AccessionNumber>DQ178100</AccessionNumber><AccessionNumber>DQ178101</AccessionNumber><AccessionNumber>DQ178102</AccessionNumber><AccessionNumber>DQ178103</AccessionNumber><AccessionNumber>DQ178104</AccessionNumber><AccessionNumber>DQ178105</AccessionNumber><AccessionNumber>DQ178106</AccessionNumber><AccessionNumber>DQ178107</AccessionNumber><AccessionNumber>DQ178108</AccessionNumber><AccessionNumber>DQ178109</AccessionNumber><AccessionNumber>DQ178110</AccessionNumber><AccessionNumber>DQ178111</AccessionNumber><AccessionNumber>DQ178112</AccessionNumber><AccessionNumber>DQ178113</AccessionNumber><AccessionNumber>DQ178114</AccessionNumber><AccessionNumber>DQ178115</AccessionNumber><AccessionNumber>DQ178116</AccessionNumber><AccessionNumber>DQ178117</AccessionNumber><AccessionNumber>DQ178118</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>10</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mycol Res</MedlineTA><NlmUniqueID>8913481</NlmUniqueID><ISSNLinking>0953-7562</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012335">RNA, Ribosomal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012340">RNA, Ribosomal, 5.8S</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004484" MajorTopicYN="N" Type="Geographic">Ecuador</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029595" MajorTopicYN="N">Orchidaceae</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012335" MajorTopicYN="N">RNA, Ribosomal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012340" MajorTopicYN="N">RNA, Ribosomal, 5.8S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2006</Year><Month>05</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2006</Year><Month>08</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2006</Year><Month>08</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>11</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>1</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>11</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17081740</ArticleId><ArticleId IdType="pii">S0953-7562(06)00256-5</ArticleId><ArticleId IdType="doi">10.1016/j.mycres.2006.08.004</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MycorrhizaeV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003125 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 003125 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MycorrhizaeV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:17081740
|texte= Diverse tulasnelloid fungi form mycorrhizas with epiphytic orchids in an Andean cloud forest.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:17081740" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a MycorrhizaeV1
| This area was generated with Dilib version V0.6.37. |  |