Green fluorescent protein expression in the symbiotic basidiomycete fungus Hebeloma cylindrosporum.
Identifieur interne : 003041 ( Main/Corpus ); précédent : 003040; suivant : 003042Green fluorescent protein expression in the symbiotic basidiomycete fungus Hebeloma cylindrosporum.
Auteurs : David Rekangalt ; Marie-Christine Verner ; Ursula Kües ; Piers James Walser ; Roland Marmeisse ; Jean-Claude Debaud ; Laurence Fraissinet-TachetSource :
- FEMS microbiology letters [ 0378-1097 ] ; 2007.
English descriptors
- KwdEn :
- Agaricales (genetics), Agaricales (growth & development), Agaricales (metabolism), Galectins (genetics), Galectins (metabolism), Gene Expression Regulation, Fungal (MeSH), Genes, Reporter (MeSH), Glucose (metabolism), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Mycology (methods), Mycorrhizae (MeSH), Recombinant Fusion Proteins (metabolism), Symbiosis (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Galectins, Green Fluorescent Proteins.
- genetics : Agaricales.
- growth & development : Agaricales.
- metabolism : Agaricales, Galectins, Glucose, Green Fluorescent Proteins, Recombinant Fusion Proteins.
- methods : Mycology.
- Gene Expression Regulation, Fungal, Genes, Reporter, Mycorrhizae, Symbiosis, Transformation, Genetic.
Abstract
The symbiotic basidiomycete Hebeloma cylindrosporum is a model fungal species used to study ectomycorrhizal symbiosis at the molecular level. In order to have a vital marker, we developed a green fluorescent protein (GFP) reporter system efficiently expressed in H. cylindrosporum using the sgfp coding region bordered by two introns fused to the saprophytic basidiomycete Coprinopsis cinerea cgl1 promoter. Expression of this reporter system was tested under different environmental conditions in two transformants, and glucose was shown to repress gfp expression. Such a reporter system will be used in plant-fungus interaction to evaluate sugar supply by the plant to the compatible mycorrhizal symbiont and to compare the expression of various genes of interest in the free-living mycelia, in the symbiotic (mycorrhizas) and the reproductive (fruit bodies) structures formed by H. cylindrosporum.
DOI: 10.1111/j.1574-6968.2006.00564.x
PubMed: 17263849
Links to Exploration step
pubmed:17263849Le document en format XML
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sort="Marmeisse, Roland" uniqKey="Marmeisse R" first="Roland" last="Marmeisse">Roland Marmeisse</name></author><author><name sortKey="Debaud, Jean Claude" sort="Debaud, Jean Claude" uniqKey="Debaud J" first="Jean-Claude" last="Debaud">Jean-Claude Debaud</name></author><author><name sortKey="Fraissinet Tachet, Laurence" sort="Fraissinet Tachet, Laurence" uniqKey="Fraissinet Tachet L" first="Laurence" last="Fraissinet-Tachet">Laurence Fraissinet-Tachet</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17263849</idno><idno type="pmid">17263849</idno><idno type="doi">10.1111/j.1574-6968.2006.00564.x</idno><idno type="wicri:Area/Main/Corpus">003041</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003041</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Green fluorescent protein expression in the symbiotic basidiomycete fungus 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Jean Claude" sort="Debaud, Jean Claude" uniqKey="Debaud J" first="Jean-Claude" last="Debaud">Jean-Claude Debaud</name></author><author><name sortKey="Fraissinet Tachet, Laurence" sort="Fraissinet Tachet, Laurence" uniqKey="Fraissinet Tachet L" first="Laurence" last="Fraissinet-Tachet">Laurence Fraissinet-Tachet</name></author></analytic><series><title level="j">FEMS microbiology letters</title><idno type="ISSN">0378-1097</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agaricales (genetics)</term><term>Agaricales (growth & development)</term><term>Agaricales (metabolism)</term><term>Galectins (genetics)</term><term>Galectins (metabolism)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Glucose (metabolism)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Mycology (methods)</term><term>Mycorrhizae (MeSH)</term><term>Recombinant Fusion Proteins (metabolism)</term><term>Symbiosis (MeSH)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Galectins</term><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agaricales</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Agaricales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Agaricales</term><term>Galectins</term><term>Glucose</term><term>Green Fluorescent Proteins</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Mycology</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Fungal</term><term>Genes, 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In order to have a vital marker, we developed a green fluorescent protein (GFP) reporter system efficiently expressed in H. cylindrosporum using the sgfp coding region bordered by two introns fused to the saprophytic basidiomycete Coprinopsis cinerea cgl1 promoter. Expression of this reporter system was tested under different environmental conditions in two transformants, and glucose was shown to repress gfp expression. Such a reporter system will be used in plant-fungus interaction to evaluate sugar supply by the plant to the compatible mycorrhizal symbiont and to compare the expression of various genes of interest in the free-living mycelia, in the symbiotic (mycorrhizas) and the reproductive (fruit bodies) structures formed by H. cylindrosporum.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17263849</PMID><DateCompleted><Year>2007</Year><Month>03</Month><Day>16</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0378-1097</ISSN><JournalIssue CitedMedium="Print"><Volume>268</Volume><Issue>1</Issue><PubDate><Year>2007</Year><Month>Mar</Month></PubDate></JournalIssue><Title>FEMS microbiology letters</Title><ISOAbbreviation>FEMS Microbiol Lett</ISOAbbreviation></Journal><ArticleTitle>Green fluorescent protein expression in the symbiotic basidiomycete fungus Hebeloma cylindrosporum.</ArticleTitle><Pagination><MedlinePgn>67-72</MedlinePgn></Pagination><Abstract><AbstractText>The symbiotic basidiomycete Hebeloma cylindrosporum is a model fungal species used to study ectomycorrhizal symbiosis at the molecular level. In order to have a vital marker, we developed a green fluorescent protein (GFP) reporter system efficiently expressed in H. cylindrosporum using the sgfp coding region bordered by two introns fused to the saprophytic basidiomycete Coprinopsis cinerea cgl1 promoter. Expression of this reporter system was tested under different environmental conditions in two transformants, and glucose was shown to repress gfp expression. Such a reporter system will be used in plant-fungus interaction to evaluate sugar supply by the plant to the compatible mycorrhizal symbiont and to compare the expression of various genes of interest in the free-living mycelia, in the symbiotic (mycorrhizas) and the reproductive (fruit bodies) structures formed by H. cylindrosporum.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rekangalt</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Université de Lyon, Université Lyon 1, Ecologie Microbienne (UMR CNRS 5557, USC INRA 1193), Villeurbanne, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Verner</LastName><ForeName>Marie-Christine</ForeName><Initials>MC</Initials></Author><Author ValidYN="Y"><LastName>Kües</LastName><ForeName>Ursula</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Walser</LastName><ForeName>Piers James</ForeName><Initials>PJ</Initials></Author><Author ValidYN="Y"><LastName>Marmeisse</LastName><ForeName>Roland</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Debaud</LastName><ForeName>Jean-Claude</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Fraissinet-Tachet</LastName><ForeName>Laurence</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEMS Microbiol Lett</MedlineTA><NlmUniqueID>7705721</NlmUniqueID><ISSNLinking>0378-1097</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009172" MajorTopicYN="N">Mycology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="Y">Symbiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>2</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>3</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>2</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId 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