Green fluorescent protein expression in the symbiotic basidiomycete fungus Hebeloma cylindrosporum.
Identifieur interne : 003041 ( Main/Corpus ); précédent : 003040; suivant : 003042Green fluorescent protein expression in the symbiotic basidiomycete fungus Hebeloma cylindrosporum.
Auteurs : David Rekangalt ; Marie-Christine Verner ; Ursula Kües ; Piers James Walser ; Roland Marmeisse ; Jean-Claude Debaud ; Laurence Fraissinet-TachetSource :
- FEMS microbiology letters [ 0378-1097 ] ; 2007.
English descriptors
- KwdEn :
- Agaricales (genetics), Agaricales (growth & development), Agaricales (metabolism), Galectins (genetics), Galectins (metabolism), Gene Expression Regulation, Fungal (MeSH), Genes, Reporter (MeSH), Glucose (metabolism), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Mycology (methods), Mycorrhizae (MeSH), Recombinant Fusion Proteins (metabolism), Symbiosis (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Galectins, Green Fluorescent Proteins.
- genetics : Agaricales.
- growth & development : Agaricales.
- metabolism : Agaricales, Galectins, Glucose, Green Fluorescent Proteins, Recombinant Fusion Proteins.
- methods : Mycology.
- Gene Expression Regulation, Fungal, Genes, Reporter, Mycorrhizae, Symbiosis, Transformation, Genetic.
Abstract
The symbiotic basidiomycete Hebeloma cylindrosporum is a model fungal species used to study ectomycorrhizal symbiosis at the molecular level. In order to have a vital marker, we developed a green fluorescent protein (GFP) reporter system efficiently expressed in H. cylindrosporum using the sgfp coding region bordered by two introns fused to the saprophytic basidiomycete Coprinopsis cinerea cgl1 promoter. Expression of this reporter system was tested under different environmental conditions in two transformants, and glucose was shown to repress gfp expression. Such a reporter system will be used in plant-fungus interaction to evaluate sugar supply by the plant to the compatible mycorrhizal symbiont and to compare the expression of various genes of interest in the free-living mycelia, in the symbiotic (mycorrhizas) and the reproductive (fruit bodies) structures formed by H. cylindrosporum.
DOI: 10.1111/j.1574-6968.2006.00564.x
PubMed: 17263849
Links to Exploration step
pubmed:17263849Le document en format XML
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<author><name sortKey="Rekangalt, David" sort="Rekangalt, David" uniqKey="Rekangalt D" first="David" last="Rekangalt">David Rekangalt</name>
<affiliation><nlm:affiliation>Université de Lyon, Université Lyon 1, Ecologie Microbienne (UMR CNRS 5557, USC INRA 1193), Villeurbanne, France.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Verner, Marie Christine" sort="Verner, Marie Christine" uniqKey="Verner M" first="Marie-Christine" last="Verner">Marie-Christine Verner</name>
</author>
<author><name sortKey="Kues, Ursula" sort="Kues, Ursula" uniqKey="Kues U" first="Ursula" last="Kües">Ursula Kües</name>
</author>
<author><name sortKey="Walser, Piers James" sort="Walser, Piers James" uniqKey="Walser P" first="Piers James" last="Walser">Piers James Walser</name>
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<author><name sortKey="Marmeisse, Roland" sort="Marmeisse, Roland" uniqKey="Marmeisse R" first="Roland" last="Marmeisse">Roland Marmeisse</name>
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<author><name sortKey="Debaud, Jean Claude" sort="Debaud, Jean Claude" uniqKey="Debaud J" first="Jean-Claude" last="Debaud">Jean-Claude Debaud</name>
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<author><name sortKey="Fraissinet Tachet, Laurence" sort="Fraissinet Tachet, Laurence" uniqKey="Fraissinet Tachet L" first="Laurence" last="Fraissinet-Tachet">Laurence Fraissinet-Tachet</name>
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<author><name sortKey="Verner, Marie Christine" sort="Verner, Marie Christine" uniqKey="Verner M" first="Marie-Christine" last="Verner">Marie-Christine Verner</name>
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<author><name sortKey="Kues, Ursula" sort="Kues, Ursula" uniqKey="Kues U" first="Ursula" last="Kües">Ursula Kües</name>
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<author><name sortKey="Walser, Piers James" sort="Walser, Piers James" uniqKey="Walser P" first="Piers James" last="Walser">Piers James Walser</name>
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<author><name sortKey="Marmeisse, Roland" sort="Marmeisse, Roland" uniqKey="Marmeisse R" first="Roland" last="Marmeisse">Roland Marmeisse</name>
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<author><name sortKey="Debaud, Jean Claude" sort="Debaud, Jean Claude" uniqKey="Debaud J" first="Jean-Claude" last="Debaud">Jean-Claude Debaud</name>
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<author><name sortKey="Fraissinet Tachet, Laurence" sort="Fraissinet Tachet, Laurence" uniqKey="Fraissinet Tachet L" first="Laurence" last="Fraissinet-Tachet">Laurence Fraissinet-Tachet</name>
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<term>Agaricales (growth & development)</term>
<term>Agaricales (metabolism)</term>
<term>Galectins (genetics)</term>
<term>Galectins (metabolism)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Genes, Reporter (MeSH)</term>
<term>Glucose (metabolism)</term>
<term>Green Fluorescent Proteins (genetics)</term>
<term>Green Fluorescent Proteins (metabolism)</term>
<term>Mycology (methods)</term>
<term>Mycorrhizae (MeSH)</term>
<term>Recombinant Fusion Proteins (metabolism)</term>
<term>Symbiosis (MeSH)</term>
<term>Transformation, Genetic (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Galectins</term>
<term>Green Fluorescent Proteins</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agaricales</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Agaricales</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Agaricales</term>
<term>Galectins</term>
<term>Glucose</term>
<term>Green Fluorescent Proteins</term>
<term>Recombinant Fusion Proteins</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Mycology</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Fungal</term>
<term>Genes, Reporter</term>
<term>Mycorrhizae</term>
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<front><div type="abstract" xml:lang="en">The symbiotic basidiomycete Hebeloma cylindrosporum is a model fungal species used to study ectomycorrhizal symbiosis at the molecular level. In order to have a vital marker, we developed a green fluorescent protein (GFP) reporter system efficiently expressed in H. cylindrosporum using the sgfp coding region bordered by two introns fused to the saprophytic basidiomycete Coprinopsis cinerea cgl1 promoter. Expression of this reporter system was tested under different environmental conditions in two transformants, and glucose was shown to repress gfp expression. Such a reporter system will be used in plant-fungus interaction to evaluate sugar supply by the plant to the compatible mycorrhizal symbiont and to compare the expression of various genes of interest in the free-living mycelia, in the symbiotic (mycorrhizas) and the reproductive (fruit bodies) structures formed by H. cylindrosporum.</div>
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<Abstract><AbstractText>The symbiotic basidiomycete Hebeloma cylindrosporum is a model fungal species used to study ectomycorrhizal symbiosis at the molecular level. In order to have a vital marker, we developed a green fluorescent protein (GFP) reporter system efficiently expressed in H. cylindrosporum using the sgfp coding region bordered by two introns fused to the saprophytic basidiomycete Coprinopsis cinerea cgl1 promoter. Expression of this reporter system was tested under different environmental conditions in two transformants, and glucose was shown to repress gfp expression. Such a reporter system will be used in plant-fungus interaction to evaluate sugar supply by the plant to the compatible mycorrhizal symbiont and to compare the expression of various genes of interest in the free-living mycelia, in the symbiotic (mycorrhizas) and the reproductive (fruit bodies) structures formed by H. cylindrosporum.</AbstractText>
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