Presymbiotic growth and sporal morphology are affected in the arbuscular mycorrhizal fungus Gigaspora margarita cured of its endobacteria.
Identifieur interne : 003015 ( Main/Corpus ); précédent : 003014; suivant : 003016Presymbiotic growth and sporal morphology are affected in the arbuscular mycorrhizal fungus Gigaspora margarita cured of its endobacteria.
Auteurs : Erica Lumini ; Valeria Bianciotto ; Patricia Jargeat ; Mara Novero ; Alessandra Salvioli ; Antonella Faccio ; Guillaume Bécard ; Paola BonfanteSource :
- Cellular microbiology [ 1462-5814 ] ; 2007.
English descriptors
- KwdEn :
- Betaproteobacteria (growth & development), Betaproteobacteria (isolation & purification), Daucus carota (microbiology), Fungi (genetics), Fungi (growth & development), Fungi (physiology), Fungi (ultrastructure), Microscopy, Electron, Transmission (MeSH), Mycorrhizae (growth & development), Mycorrhizae (ultrastructure), Plant Roots (microbiology), Serial Passage (MeSH), Spores, Fungal (genetics), Spores, Fungal (physiology), Symbiosis (MeSH).
- MESH :
- genetics : Fungi, Spores, Fungal.
- growth & development : Betaproteobacteria, Fungi, Mycorrhizae.
- isolation & purification : Betaproteobacteria.
- microbiology : Daucus carota, Plant Roots.
- physiology : Fungi, Spores, Fungal.
- ultrastructure : Fungi, Mycorrhizae.
- Microscopy, Electron, Transmission, Serial Passage, Symbiosis.
Abstract
Some arbuscular mycorrhizal fungi contain endocellular bacteria. In Gigaspora margarita BEG 34, a homogenous population of beta-Proteobacteria is hosted inside the fungal spore. The bacteria, named Candidatus Glomeribacter gigasporarum, are vertically transmitted through fungal spore generations. Here we report how a protocol based on repeated passages through single-spore inocula caused dilution of the initial bacterial population eventually leading to cured spores. Spores of this line had a distinct phenotype regarding cytoplasm organization, vacuole morphology, cell wall organization, lipid bodies and pigment granules. The absence of bacteria severely affected presymbiotic fungal growth such as hyphal elongation and branching after root exudate treatment, suggesting that Ca. Glomeribacter gigasporarum is important for optimal development of its fungal host. Under laboratory conditions, the cured fungus could be propagated, i.e. could form mycorrhizae and sporulate, and can therefore be considered as a stable variant of the wild type. The results demonstrated that - at least for the G. margarita BEG 34 isolate - the absence of endobacteria affects the spore phenotype of the fungal host, and causes delays in the growth of germinating mycelium, possibly affecting its ecological fitness. This cured line is the first manipulated and stable isolate of an arbuscular mycorrhizal fungus.
DOI: 10.1111/j.1462-5822.2007.00907.x
PubMed: 17331157
Links to Exploration step
pubmed:17331157Le document en format XML
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CNR, Viale Mattioli 25, 10125-I, Torino, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Bianciotto, Valeria" sort="Bianciotto, Valeria" uniqKey="Bianciotto V" first="Valeria" last="Bianciotto">Valeria Bianciotto</name></author><author><name sortKey="Jargeat, Patricia" sort="Jargeat, Patricia" uniqKey="Jargeat P" first="Patricia" last="Jargeat">Patricia Jargeat</name></author><author><name sortKey="Novero, Mara" sort="Novero, Mara" uniqKey="Novero M" first="Mara" last="Novero">Mara Novero</name></author><author><name sortKey="Salvioli, Alessandra" sort="Salvioli, Alessandra" uniqKey="Salvioli A" first="Alessandra" last="Salvioli">Alessandra Salvioli</name></author><author><name sortKey="Faccio, Antonella" sort="Faccio, Antonella" uniqKey="Faccio A" first="Antonella" last="Faccio">Antonella Faccio</name></author><author><name sortKey="Becard, Guillaume" sort="Becard, Guillaume" uniqKey="Becard G" first="Guillaume" last="Bécard">Guillaume Bécard</name></author><author><name sortKey="Bonfante, Paola" sort="Bonfante, Paola" uniqKey="Bonfante P" first="Paola" last="Bonfante">Paola Bonfante</name></author></analytic><series><title level="j">Cellular microbiology</title><idno type="ISSN">1462-5814</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Betaproteobacteria (growth & development)</term><term>Betaproteobacteria (isolation & purification)</term><term>Daucus carota (microbiology)</term><term>Fungi (genetics)</term><term>Fungi (growth & development)</term><term>Fungi (physiology)</term><term>Fungi (ultrastructure)</term><term>Microscopy, Electron, Transmission (MeSH)</term><term>Mycorrhizae (growth & development)</term><term>Mycorrhizae (ultrastructure)</term><term>Plant Roots (microbiology)</term><term>Serial Passage (MeSH)</term><term>Spores, Fungal (genetics)</term><term>Spores, Fungal (physiology)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fungi</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Betaproteobacteria</term><term>Fungi</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Betaproteobacteria</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Daucus carota</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fungi</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Fungi</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Microscopy, Electron, Transmission</term><term>Serial Passage</term><term>Symbiosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Some arbuscular mycorrhizal fungi contain endocellular bacteria. In Gigaspora margarita BEG 34, a homogenous population of beta-Proteobacteria is hosted inside the fungal spore. The bacteria, named Candidatus Glomeribacter gigasporarum, are vertically transmitted through fungal spore generations. Here we report how a protocol based on repeated passages through single-spore inocula caused dilution of the initial bacterial population eventually leading to cured spores. Spores of this line had a distinct phenotype regarding cytoplasm organization, vacuole morphology, cell wall organization, lipid bodies and pigment granules. The absence of bacteria severely affected presymbiotic fungal growth such as hyphal elongation and branching after root exudate treatment, suggesting that Ca. Glomeribacter gigasporarum is important for optimal development of its fungal host. Under laboratory conditions, the cured fungus could be propagated, i.e. could form mycorrhizae and sporulate, and can therefore be considered as a stable variant of the wild type. The results demonstrated that - at least for the G. margarita BEG 34 isolate - the absence of endobacteria affects the spore phenotype of the fungal host, and causes delays in the growth of germinating mycelium, possibly affecting its ecological fitness. This cured line is the first manipulated and stable isolate of an arbuscular mycorrhizal fungus.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17331157</PMID><DateCompleted><Year>2007</Year><Month>08</Month><Day>21</Day></DateCompleted><DateRevised><Year>2007</Year><Month>06</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1462-5814</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>7</Issue><PubDate><Year>2007</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Cellular microbiology</Title><ISOAbbreviation>Cell Microbiol</ISOAbbreviation></Journal><ArticleTitle>Presymbiotic growth and sporal morphology are affected in the arbuscular mycorrhizal fungus Gigaspora margarita cured of its endobacteria.</ArticleTitle><Pagination><MedlinePgn>1716-29</MedlinePgn></Pagination><Abstract><AbstractText>Some arbuscular mycorrhizal fungi contain endocellular bacteria. In Gigaspora margarita BEG 34, a homogenous population of beta-Proteobacteria is hosted inside the fungal spore. The bacteria, named Candidatus Glomeribacter gigasporarum, are vertically transmitted through fungal spore generations. Here we report how a protocol based on repeated passages through single-spore inocula caused dilution of the initial bacterial population eventually leading to cured spores. Spores of this line had a distinct phenotype regarding cytoplasm organization, vacuole morphology, cell wall organization, lipid bodies and pigment granules. The absence of bacteria severely affected presymbiotic fungal growth such as hyphal elongation and branching after root exudate treatment, suggesting that Ca. Glomeribacter gigasporarum is important for optimal development of its fungal host. Under laboratory conditions, the cured fungus could be propagated, i.e. could form mycorrhizae and sporulate, and can therefore be considered as a stable variant of the wild type. The results demonstrated that - at least for the G. margarita BEG 34 isolate - the absence of endobacteria affects the spore phenotype of the fungal host, and causes delays in the growth of germinating mycelium, possibly affecting its ecological fitness. This cured line is the first manipulated and stable isolate of an arbuscular mycorrhizal fungus.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lumini</LastName><ForeName>Erica</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Dipartimento di Biologia Vegetale dell'Università and Istituto per la Protezione delle Piante - CNR, Viale Mattioli 25, 10125-I, Torino, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bianciotto</LastName><ForeName>Valeria</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Jargeat</LastName><ForeName>Patricia</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Novero</LastName><ForeName>Mara</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Salvioli</LastName><ForeName>Alessandra</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Faccio</LastName><ForeName>Antonella</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Bécard</LastName><ForeName>Guillaume</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Bonfante</LastName><ForeName>Paola</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>02</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Cell Microbiol</MedlineTA><NlmUniqueID>100883691</NlmUniqueID><ISSNLinking>1462-5814</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020562" MajorTopicYN="N">Betaproteobacteria</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018552" MajorTopicYN="N">Daucus carota</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="Y">Mycorrhizae</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012692" MajorTopicYN="N">Serial Passage</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013172" MajorTopicYN="N">Spores, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="Y">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>3</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>8</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>3</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17331157</ArticleId><ArticleId IdType="pii">CMI907</ArticleId><ArticleId IdType="doi">10.1111/j.1462-5822.2007.00907.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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