Increased trehalose biosynthesis in Hartig net hyphae of ectomycorrhizas.
Identifieur interne : 002F86 ( Main/Corpus ); précédent : 002F85; suivant : 002F87Increased trehalose biosynthesis in Hartig net hyphae of ectomycorrhizas.
Auteurs : M Nica Fajardo L Pez ; Philipp M Nner ; Anita Willmann ; Rüdiger Hampp ; Uwe NehlsSource :
- The New phytologist [ 0028-646X ] ; 2007.
English descriptors
- KwdEn :
- Amanita (enzymology), Amanita (genetics), Amanita (metabolism), Amino Acid Sequence (MeSH), Carbohydrate Metabolism (MeSH), Gene Expression (MeSH), Glucosyltransferases (genetics), Glucosyltransferases (metabolism), Hyphae (enzymology), Hyphae (metabolism), Mycorrhizae (enzymology), Mycorrhizae (metabolism), Nitrogen (metabolism), Phosphoric Monoester Hydrolases (genetics), Phosphoric Monoester Hydrolases (metabolism), Populus (microbiology), Populus (physiology), Sequence Analysis, DNA (MeSH), Symbiosis (physiology), Trehalose (biosynthesis), Trehalose (metabolism).
- MESH :
- chemical , biosynthesis : Trehalose.
- chemical , genetics : Glucosyltransferases, Phosphoric Monoester Hydrolases.
- enzymology : Amanita, Hyphae, Mycorrhizae.
- genetics : Amanita.
- metabolism : Amanita, Glucosyltransferases, Hyphae, Mycorrhizae, Nitrogen, Phosphoric Monoester Hydrolases, Trehalose.
- microbiology : Populus.
- physiology : Populus, Symbiosis.
- Amino Acid Sequence, Carbohydrate Metabolism, Gene Expression, Sequence Analysis, DNA.
Abstract
To obtain photoassimilates in ectomycorrhizal symbiosis, the fungus has to create a strong sink, for example, by conversion of plant-derived hexoses into fungus-specific compounds. Trehalose is present in large quantities in Amanita muscaria and may thus constitute an important carbon sink. In Amanita muscaria-poplar (Populus tremula x tremuloides) ectomycorrhizas, the transcript abundances of genes encoding key enzymes of fungal trehalose biosynthesis, namely trehalose-6-phosphate synthase (TPS), trehalose-6-phosphate phosphatase (TPP) and trehalose phosphorylase (TP), were increased. When mycorrhizas were separated into mantle and Hartig net, TPS, TPP and TP expression was specifically enhanced in Hartig net hyphae. Compared with the extraradical mycelium, TPS and TPP expression was only slightly increased in the fungal sheath, while the increase in the expression of TP was more pronounced. TPS enzyme activity was also elevated in Hartig net hyphae, displaying a direct correlation between transcript abundance and turnover rate. In accordance with enhanced gene expression and TPS activity, trehalose content was 2.7 times higher in the Hartig net. The enhanced trehalose biosynthesis at the plant-fungus interface indicates that trehalose is a relevant carbohydrate sink in symbiosis. As sugar and nitrogen supply affected gene expression only slightly, the strongly increased expression of the investigated genes in mycorrhizas is presumably developmentally regulated.
DOI: 10.1111/j.1469-8137.2007.01983.x
PubMed: 17388901
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pubmed:17388901Le document en format XML
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Trehalose is present in large quantities in Amanita muscaria and may thus constitute an important carbon sink. In Amanita muscaria-poplar (Populus tremula x tremuloides) ectomycorrhizas, the transcript abundances of genes encoding key enzymes of fungal trehalose biosynthesis, namely trehalose-6-phosphate synthase (TPS), trehalose-6-phosphate phosphatase (TPP) and trehalose phosphorylase (TP), were increased. When mycorrhizas were separated into mantle and Hartig net, TPS, TPP and TP expression was specifically enhanced in Hartig net hyphae. Compared with the extraradical mycelium, TPS and TPP expression was only slightly increased in the fungal sheath, while the increase in the expression of TP was more pronounced. TPS enzyme activity was also elevated in Hartig net hyphae, displaying a direct correlation between transcript abundance and turnover rate. In accordance with enhanced gene expression and TPS activity, trehalose content was 2.7 times higher in the Hartig net. The enhanced trehalose biosynthesis at the plant-fungus interface indicates that trehalose is a relevant carbohydrate sink in symbiosis. As sugar and nitrogen supply affected gene expression only slightly, the strongly increased expression of the investigated genes in mycorrhizas is presumably developmentally regulated.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17388901</PMID><DateCompleted><Year>2007</Year><Month>06</Month><Day>18</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0028-646X</ISSN><JournalIssue CitedMedium="Print"><Volume>174</Volume><Issue>2</Issue><PubDate><Year>2007</Year></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Increased trehalose biosynthesis in Hartig net hyphae of ectomycorrhizas.</ArticleTitle><Pagination><MedlinePgn>389-98</MedlinePgn></Pagination><Abstract><AbstractText>To obtain photoassimilates in ectomycorrhizal symbiosis, the fungus has to create a strong sink, for example, by conversion of plant-derived hexoses into fungus-specific compounds. Trehalose is present in large quantities in Amanita muscaria and may thus constitute an important carbon sink. In Amanita muscaria-poplar (Populus tremula x tremuloides) ectomycorrhizas, the transcript abundances of genes encoding key enzymes of fungal trehalose biosynthesis, namely trehalose-6-phosphate synthase (TPS), trehalose-6-phosphate phosphatase (TPP) and trehalose phosphorylase (TP), were increased. When mycorrhizas were separated into mantle and Hartig net, TPS, TPP and TP expression was specifically enhanced in Hartig net hyphae. Compared with the extraradical mycelium, TPS and TPP expression was only slightly increased in the fungal sheath, while the increase in the expression of TP was more pronounced. TPS enzyme activity was also elevated in Hartig net hyphae, displaying a direct correlation between transcript abundance and turnover rate. In accordance with enhanced gene expression and TPS activity, trehalose content was 2.7 times higher in the Hartig net. The enhanced trehalose biosynthesis at the plant-fungus interface indicates that trehalose is a relevant carbohydrate sink in symbiosis. As sugar and nitrogen supply affected gene expression only slightly, the strongly increased expression of the investigated genes in mycorrhizas is presumably developmentally regulated.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>López</LastName><ForeName>Mónica Fajardo</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>Eberhard Karls Universität, Physiologische Okologie der Pflanzen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Männer</LastName><ForeName>Philipp</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Willmann</LastName><ForeName>Anita</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Hampp</LastName><ForeName>Rüdiger</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Nehls</LastName><ForeName>Uwe</ForeName><Initials>U</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>B8WCK70T7I</RegistryNumber><NameOfSubstance UI="D014199">Trehalose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="D005964">Glucosyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.15</RegistryNumber><NameOfSubstance UI="C023180">trehalose-6-phosphate synthase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.64</RegistryNumber><NameOfSubstance UI="C023181">alpha,alpha-trehalose phosphorylase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.3.12</RegistryNumber><NameOfSubstance UI="C023178">trehalose-phosphatase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.3.2</RegistryNumber><NameOfSubstance UI="D010744">Phosphoric Monoester Hydrolases</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>New Phytol. 2007;174(2):228-30</RefSource><PMID Version="1">17388882</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000545" MajorTopicYN="N">Amanita</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="N">Carbohydrate Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005964" MajorTopicYN="N">Glucosyltransferases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010744" MajorTopicYN="N">Phosphoric Monoester Hydrolases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014199" MajorTopicYN="N">Trehalose</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>3</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>6</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>3</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17388901</ArticleId><ArticleId IdType="pii">NPH1983</ArticleId><ArticleId 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