Isolation and analysis of bacteria associated with spores of Gigaspora margarita.
Identifieur interne : 002D63 ( Main/Corpus ); précédent : 002D62; suivant : 002D64Isolation and analysis of bacteria associated with spores of Gigaspora margarita.
Auteurs : A F Cruz ; S. Horii ; S. Ochiai ; A. Yasuda ; T. IshiiSource :
- Journal of applied microbiology [ 1365-2672 ] ; 2008.
English descriptors
- KwdEn :
- Antibiosis (MeSH), Bacteria (genetics), Bacteria (isolation & purification), Bacteria (ultrastructure), Gram-Negative Bacteria (genetics), Gram-Negative Bacteria (isolation & purification), Gram-Negative Bacteria (ultrastructure), Gram-Positive Bacteria (genetics), Gram-Positive Bacteria (isolation & purification), Gram-Positive Bacteria (ultrastructure), Mycorrhizae (physiology), Phylogeny (MeSH), Polymerase Chain Reaction (methods), RNA, Ribosomal, 16S (analysis), Soil Microbiology (MeSH), Spores (MeSH), Symbiosis (MeSH).
- MESH :
- chemical , analysis : RNA, Ribosomal, 16S.
- genetics : Bacteria, Gram-Negative Bacteria, Gram-Positive Bacteria.
- isolation & purification : Bacteria, Gram-Negative Bacteria, Gram-Positive Bacteria.
- methods : Polymerase Chain Reaction.
- physiology : Mycorrhizae.
- ultrastructure : Bacteria, Gram-Negative Bacteria, Gram-Positive Bacteria.
- Antibiosis, Phylogeny, Soil Microbiology, Spores, Symbiosis.
Abstract
AIMS
The aim of this work was to observe bacteria associated with the spores of Gigaspora margarita, an arbuscular mycorrhizal fungus (AMF).
METHODS AND RESULTS
First, a direct analysis of DNA from sterilized spores indicated the bacteria belonging to the genus Janthinobacterium. In the second assay, two bacterial strains were isolated by osmosis from protoplasts, which were derived from spores by using two particular enzymes: lysing enzymes and yatalase. After isolation, cultivation and identification by their DNA as performed in the first experiment, the species with the closest relation were Janthinobacterium lividum (KCIGM01) and Paenibacillus polymyxa (KCIGM04) isolated with lysing enzymes and yatalase respectively. Morphologically, J. lividum was Gram negative and oval, while P. polymyxa was also oval, but Gram positive. Both strains had antagonistic effects to the pathogenic fungi Rosellimia necatrix, Pythium ultimum, Fusarium oxysporum and Rhizoctonia solani. In particular, J. lividum was much stronger in this role. However, in phosphorus (P) solubilization P. polymyxa functioned better than J. lividum.
CONCLUSIONS
This experiment had revealed two new bacteria species (P. polymyxa and J. lividum), associated with AMF spores, which functioned to suppress diseases and to solubilize P.
SIGNIFICANCE AND IMPACT OF THE STUDY
AMF spores could be a useful source for bacterial antagonists to soil-borne diseases and P solubilization.
DOI: 10.1111/j.1365-2672.2007.03695.x
PubMed: 18217929
Links to Exploration step
pubmed:18217929Le document en format XML
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Ishii</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18217929</idno><idno type="pmid">18217929</idno><idno type="doi">10.1111/j.1365-2672.2007.03695.x</idno><idno type="wicri:Area/Main/Corpus">002D63</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002D63</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Isolation and analysis of bacteria associated with spores of Gigaspora margarita.</title><author><name sortKey="Cruz, A F" sort="Cruz, A F" uniqKey="Cruz A" first="A F" last="Cruz">A F Cruz</name><affiliation><nlm:affiliation>Graduate School of Agriculture, Kyoto Prefectural University, Sakyo-ku, Kyoto, Japan. andre@kpu.ac.jp</nlm:affiliation></affiliation></author><author><name sortKey="Horii, S" sort="Horii, S" uniqKey="Horii S" first="S" last="Horii">S. Horii</name></author><author><name sortKey="Ochiai, S" sort="Ochiai, S" uniqKey="Ochiai S" first="S" last="Ochiai">S. Ochiai</name></author><author><name sortKey="Yasuda, A" sort="Yasuda, A" uniqKey="Yasuda A" first="A" last="Yasuda">A. Yasuda</name></author><author><name sortKey="Ishii, T" sort="Ishii, T" uniqKey="Ishii T" first="T" last="Ishii">T. Ishii</name></author></analytic><series><title level="j">Journal of applied microbiology</title><idno type="eISSN">1365-2672</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibiosis (MeSH)</term><term>Bacteria (genetics)</term><term>Bacteria (isolation & purification)</term><term>Bacteria (ultrastructure)</term><term>Gram-Negative Bacteria (genetics)</term><term>Gram-Negative Bacteria (isolation & purification)</term><term>Gram-Negative Bacteria (ultrastructure)</term><term>Gram-Positive Bacteria (genetics)</term><term>Gram-Positive Bacteria (isolation & purification)</term><term>Gram-Positive Bacteria (ultrastructure)</term><term>Mycorrhizae (physiology)</term><term>Phylogeny (MeSH)</term><term>Polymerase Chain Reaction (methods)</term><term>RNA, Ribosomal, 16S (analysis)</term><term>Soil Microbiology (MeSH)</term><term>Spores (MeSH)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteria</term><term>Gram-Negative Bacteria</term><term>Gram-Positive Bacteria</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term><term>Gram-Negative Bacteria</term><term>Gram-Positive Bacteria</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Bacteria</term><term>Gram-Negative Bacteria</term><term>Gram-Positive Bacteria</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Antibiosis</term><term>Phylogeny</term><term>Soil Microbiology</term><term>Spores</term><term>Symbiosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>AIMS</b></p><p>The aim of this work was to observe bacteria associated with the spores of Gigaspora margarita, an arbuscular mycorrhizal fungus (AMF).</p></div><div type="abstract" xml:lang="en"><p><b>METHODS AND RESULTS</b></p><p>First, a direct analysis of DNA from sterilized spores indicated the bacteria belonging to the genus Janthinobacterium. In the second assay, two bacterial strains were isolated by osmosis from protoplasts, which were derived from spores by using two particular enzymes: lysing enzymes and yatalase. After isolation, cultivation and identification by their DNA as performed in the first experiment, the species with the closest relation were Janthinobacterium lividum (KCIGM01) and Paenibacillus polymyxa (KCIGM04) isolated with lysing enzymes and yatalase respectively. Morphologically, J. lividum was Gram negative and oval, while P. polymyxa was also oval, but Gram positive. Both strains had antagonistic effects to the pathogenic fungi Rosellimia necatrix, Pythium ultimum, Fusarium oxysporum and Rhizoctonia solani. In particular, J. lividum was much stronger in this role. However, in phosphorus (P) solubilization P. polymyxa functioned better than J. lividum.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>This experiment had revealed two new bacteria species (P. polymyxa and J. lividum), associated with AMF spores, which functioned to suppress diseases and to solubilize P.</p></div><div type="abstract" xml:lang="en"><p><b>SIGNIFICANCE AND IMPACT OF THE STUDY</b></p><p>AMF spores could be a useful source for bacterial antagonists to soil-borne diseases and P solubilization.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18217929</PMID><DateCompleted><Year>2008</Year><Month>10</Month><Day>10</Day></DateCompleted><DateRevised><Year>2008</Year><Month>05</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-2672</ISSN><JournalIssue CitedMedium="Internet"><Volume>104</Volume><Issue>6</Issue><PubDate><Year>2008</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of applied microbiology</Title><ISOAbbreviation>J Appl Microbiol</ISOAbbreviation></Journal><ArticleTitle>Isolation and analysis of bacteria associated with spores of Gigaspora margarita.</ArticleTitle><Pagination><MedlinePgn>1711-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1365-2672.2007.03695.x</ELocationID><Abstract><AbstractText Label="AIMS" NlmCategory="OBJECTIVE">The aim of this work was to observe bacteria associated with the spores of Gigaspora margarita, an arbuscular mycorrhizal fungus (AMF).</AbstractText><AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">First, a direct analysis of DNA from sterilized spores indicated the bacteria belonging to the genus Janthinobacterium. In the second assay, two bacterial strains were isolated by osmosis from protoplasts, which were derived from spores by using two particular enzymes: lysing enzymes and yatalase. After isolation, cultivation and identification by their DNA as performed in the first experiment, the species with the closest relation were Janthinobacterium lividum (KCIGM01) and Paenibacillus polymyxa (KCIGM04) isolated with lysing enzymes and yatalase respectively. Morphologically, J. lividum was Gram negative and oval, while P. polymyxa was also oval, but Gram positive. Both strains had antagonistic effects to the pathogenic fungi Rosellimia necatrix, Pythium ultimum, Fusarium oxysporum and Rhizoctonia solani. In particular, J. lividum was much stronger in this role. However, in phosphorus (P) solubilization P. polymyxa functioned better than J. lividum.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">This experiment had revealed two new bacteria species (P. polymyxa and J. lividum), associated with AMF spores, which functioned to suppress diseases and to solubilize P.</AbstractText><AbstractText Label="SIGNIFICANCE AND IMPACT OF THE STUDY" NlmCategory="CONCLUSIONS">AMF spores could be a useful source for bacterial antagonists to soil-borne diseases and P solubilization.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cruz</LastName><ForeName>A F</ForeName><Initials>AF</Initials><AffiliationInfo><Affiliation>Graduate School of Agriculture, Kyoto Prefectural University, Sakyo-ku, Kyoto, Japan. andre@kpu.ac.jp</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Horii</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Ochiai</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Yasuda</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Ishii</LastName><ForeName>T</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>01</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Appl Microbiol</MedlineTA><NlmUniqueID>9706280</NlmUniqueID><ISSNLinking>1364-5072</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000898" MajorTopicYN="N">Antibiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006090" MajorTopicYN="N">Gram-Negative Bacteria</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006094" MajorTopicYN="N">Gram-Positive Bacteria</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013170" MajorTopicYN="N">Spores</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>1</Month><Day>26</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>10</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>1</Month><Day>26</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18217929</ArticleId><ArticleId IdType="pii">JAM3695</ArticleId><ArticleId IdType="doi">10.1111/j.1365-2672.2007.03695.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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