Ultrastructural localization of heavy metals in the extraradical mycelium and spores of the arbuscular mycorrhizal fungus Glomus intraradices.
Identifieur interne : 002D06 ( Main/Corpus ); précédent : 002D05; suivant : 002D07Ultrastructural localization of heavy metals in the extraradical mycelium and spores of the arbuscular mycorrhizal fungus Glomus intraradices.
Auteurs : Manuel González-Guerrero ; Lewis H. Melville ; Nuria Ferrol ; John N A. Lott ; Concepci N Azc N-Aguilar ; R Larry PetersonSource :
- Canadian journal of microbiology [ 0008-4166 ] ; 2008.
English descriptors
- KwdEn :
- Cadmium (metabolism), Cell Wall (chemistry), Copper (metabolism), Cytoplasm (chemistry), Fungi (chemistry), Fungi (physiology), Fungi (ultrastructure), Metals, Heavy (analysis), Microscopy, Electron, Transmission (MeSH), Mycelium (chemistry), Mycelium (ultrastructure), Mycorrhizae (chemistry), Mycorrhizae (physiology), Mycorrhizae (ultrastructure), Spectrometry, X-Ray Emission (MeSH), Spores, Fungal (chemistry), Spores, Fungal (ultrastructure), Time Factors (MeSH), Vacuoles (chemistry), Zinc (metabolism).
- MESH :
- chemical , analysis : Metals, Heavy.
- chemical , metabolism : Cadmium, Copper, Zinc.
- chemistry : Cell Wall, Cytoplasm, Fungi, Mycelium, Mycorrhizae, Spores, Fungal, Vacuoles.
- physiology : Fungi, Mycorrhizae.
- ultrastructure : Fungi, Mycelium, Mycorrhizae, Spores, Fungal.
- Microscopy, Electron, Transmission, Spectrometry, X-Ray Emission, Time Factors.
Abstract
Arbuscular mycorrhizal fungi, obligate symbionts of most plant species, are able to accumulate heavy metals, thereby, protecting plants from metal toxicity. In this study, the ultrastructural localization of Zn, Cu, and Cd in the extraradical mycelium and spores of the arbuscular mycorrhizal fungus Glomus intraradices grown in monoxenic cultures was investigated. Zinc, Cu, or Cd was applied to the extraradical mycelium to final concentrations of 7.5, 5.0, or 0.45 mmol/L, respectively. Samples were collected at time 0, 8 h, and 7 days after metal application and were prepared for rapid freezing and freeze substitution. Metal content in different subcellular locations (wall, cytoplasm, and vacuoles), both in hyphae and spores, was determined by energy-dispersive X-ray spectroscopy. In all treatments and fungal structures analysed, heavy metals accumulated mainly in the fungal cell wall and in the vacuoles, while minor changes in metal concentrations were detected in the cytoplasm. Incorporation of Zn into the fungus occurred during the first 8 h after metal addition with no subsequent accumulation. On the other hand, Cu steadily accumulated in the spore vacuoles over time, whereas Cd steadily accumulated in the hyphal vacuoles. These results suggest that binding of metals to the cell walls and compartmentalization in vacuoles may be essential mechanisms for metal detoxification.
DOI: 10.1139/w07-119
PubMed: 18388979
Links to Exploration step
pubmed:18388979Le document en format XML
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Melville</name></author><author><name sortKey="Ferrol, Nuria" sort="Ferrol, Nuria" uniqKey="Ferrol N" first="Nuria" last="Ferrol">Nuria Ferrol</name></author><author><name sortKey="Lott, John N A" sort="Lott, John N A" uniqKey="Lott J" first="John N A" last="Lott">John N A. Lott</name></author><author><name sortKey="Azc N Aguilar, Concepci N" sort="Azc N Aguilar, Concepci N" uniqKey="Azc N Aguilar C" first="Concepci N" last="Azc N-Aguilar">Concepci N Azc N-Aguilar</name></author><author><name sortKey="Peterson, R Larry" sort="Peterson, R Larry" uniqKey="Peterson R" first="R Larry" last="Peterson">R Larry Peterson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18388979</idno><idno type="pmid">18388979</idno><idno type="doi">10.1139/w07-119</idno><idno type="wicri:Area/Main/Corpus">002D06</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002D06</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ultrastructural localization of heavy metals in the extraradical mycelium and spores of the arbuscular mycorrhizal fungus Glomus intraradices.</title><author><name sortKey="Gonzalez Guerrero, Manuel" sort="Gonzalez Guerrero, Manuel" uniqKey="Gonzalez Guerrero M" first="Manuel" last="González-Guerrero">Manuel González-Guerrero</name><affiliation><nlm:affiliation>Departamento de Microbiologia del Suelo y Sistemas Simbioticos, Estacion Experimental del Zaidin, Consejo Supertableior de Investigaciones Cientificas (CSIC), Profesor Albareda 1, 18008 Granada, Spain. manuelgg@wpi.edu</nlm:affiliation></affiliation></author><author><name sortKey="Melville, Lewis H" sort="Melville, Lewis H" uniqKey="Melville L" first="Lewis H" last="Melville">Lewis H. 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Lott</name></author><author><name sortKey="Azc N Aguilar, Concepci N" sort="Azc N Aguilar, Concepci N" uniqKey="Azc N Aguilar C" first="Concepci N" last="Azc N-Aguilar">Concepci N Azc N-Aguilar</name></author><author><name sortKey="Peterson, R Larry" sort="Peterson, R Larry" uniqKey="Peterson R" first="R Larry" last="Peterson">R Larry Peterson</name></author></analytic><series><title level="j">Canadian journal of microbiology</title><idno type="ISSN">0008-4166</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cadmium (metabolism)</term><term>Cell Wall (chemistry)</term><term>Copper (metabolism)</term><term>Cytoplasm (chemistry)</term><term>Fungi (chemistry)</term><term>Fungi (physiology)</term><term>Fungi (ultrastructure)</term><term>Metals, Heavy (analysis)</term><term>Microscopy, Electron, Transmission (MeSH)</term><term>Mycelium (chemistry)</term><term>Mycelium (ultrastructure)</term><term>Mycorrhizae (chemistry)</term><term>Mycorrhizae (physiology)</term><term>Mycorrhizae (ultrastructure)</term><term>Spectrometry, X-Ray Emission (MeSH)</term><term>Spores, Fungal (chemistry)</term><term>Spores, Fungal (ultrastructure)</term><term>Time Factors (MeSH)</term><term>Vacuoles (chemistry)</term><term>Zinc (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Metals, Heavy</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cadmium</term><term>Copper</term><term>Zinc</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Wall</term><term>Cytoplasm</term><term>Fungi</term><term>Mycelium</term><term>Mycorrhizae</term><term>Spores, Fungal</term><term>Vacuoles</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fungi</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Fungi</term><term>Mycelium</term><term>Mycorrhizae</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Microscopy, Electron, Transmission</term><term>Spectrometry, X-Ray Emission</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Arbuscular mycorrhizal fungi, obligate symbionts of most plant species, are able to accumulate heavy metals, thereby, protecting plants from metal toxicity. In this study, the ultrastructural localization of Zn, Cu, and Cd in the extraradical mycelium and spores of the arbuscular mycorrhizal fungus Glomus intraradices grown in monoxenic cultures was investigated. Zinc, Cu, or Cd was applied to the extraradical mycelium to final concentrations of 7.5, 5.0, or 0.45 mmol/L, respectively. Samples were collected at time 0, 8 h, and 7 days after metal application and were prepared for rapid freezing and freeze substitution. Metal content in different subcellular locations (wall, cytoplasm, and vacuoles), both in hyphae and spores, was determined by energy-dispersive X-ray spectroscopy. In all treatments and fungal structures analysed, heavy metals accumulated mainly in the fungal cell wall and in the vacuoles, while minor changes in metal concentrations were detected in the cytoplasm. Incorporation of Zn into the fungus occurred during the first 8 h after metal addition with no subsequent accumulation. On the other hand, Cu steadily accumulated in the spore vacuoles over time, whereas Cd steadily accumulated in the hyphal vacuoles. These results suggest that binding of metals to the cell walls and compartmentalization in vacuoles may be essential mechanisms for metal detoxification.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18388979</PMID><DateCompleted><Year>2008</Year><Month>05</Month><Day>22</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0008-4166</ISSN><JournalIssue CitedMedium="Print"><Volume>54</Volume><Issue>2</Issue><PubDate><Year>2008</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Canadian journal of microbiology</Title><ISOAbbreviation>Can J Microbiol</ISOAbbreviation></Journal><ArticleTitle>Ultrastructural localization of heavy metals in the extraradical mycelium and spores of the arbuscular mycorrhizal fungus Glomus intraradices.</ArticleTitle><Pagination><MedlinePgn>103-10</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1139/w07-119</ELocationID><Abstract><AbstractText>Arbuscular mycorrhizal fungi, obligate symbionts of most plant species, are able to accumulate heavy metals, thereby, protecting plants from metal toxicity. In this study, the ultrastructural localization of Zn, Cu, and Cd in the extraradical mycelium and spores of the arbuscular mycorrhizal fungus Glomus intraradices grown in monoxenic cultures was investigated. Zinc, Cu, or Cd was applied to the extraradical mycelium to final concentrations of 7.5, 5.0, or 0.45 mmol/L, respectively. Samples were collected at time 0, 8 h, and 7 days after metal application and were prepared for rapid freezing and freeze substitution. Metal content in different subcellular locations (wall, cytoplasm, and vacuoles), both in hyphae and spores, was determined by energy-dispersive X-ray spectroscopy. In all treatments and fungal structures analysed, heavy metals accumulated mainly in the fungal cell wall and in the vacuoles, while minor changes in metal concentrations were detected in the cytoplasm. Incorporation of Zn into the fungus occurred during the first 8 h after metal addition with no subsequent accumulation. On the other hand, Cu steadily accumulated in the spore vacuoles over time, whereas Cd steadily accumulated in the hyphal vacuoles. These results suggest that binding of metals to the cell walls and compartmentalization in vacuoles may be essential mechanisms for metal detoxification.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>González-Guerrero</LastName><ForeName>Manuel</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Departamento de Microbiologia del Suelo y Sistemas Simbioticos, Estacion Experimental del Zaidin, Consejo Supertableior de Investigaciones Cientificas (CSIC), Profesor Albareda 1, 18008 Granada, Spain. manuelgg@wpi.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Melville</LastName><ForeName>Lewis H</ForeName><Initials>LH</Initials></Author><Author ValidYN="Y"><LastName>Ferrol</LastName><ForeName>Nuria</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Lott</LastName><ForeName>John N A</ForeName><Initials>JN</Initials></Author><Author ValidYN="Y"><LastName>Azcón-Aguilar</LastName><ForeName>Concepción</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Peterson</LastName><ForeName>R Larry</ForeName><Initials>RL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Can J Microbiol</MedlineTA><NlmUniqueID>0372707</NlmUniqueID><ISSNLinking>0008-4166</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance></Chemical><Chemical><RegistryNumber>00BH33GNGH</RegistryNumber><NameOfSubstance UI="D002104">Cadmium</NameOfSubstance></Chemical><Chemical><RegistryNumber>789U1901C5</RegistryNumber><NameOfSubstance 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UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013052" MajorTopicYN="N">Spectrometry, X-Ray Emission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013172" MajorTopicYN="N">Spores, Fungal</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014617" MajorTopicYN="N">Vacuoles</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015032" MajorTopicYN="N">Zinc</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate 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