Multiple species of ectomycorrhizal fungi are frequently detected on individual oak root tips in a tropical cloud forest.
Identifieur interne : 002C03 ( Main/Corpus ); précédent : 002C02; suivant : 002C04Multiple species of ectomycorrhizal fungi are frequently detected on individual oak root tips in a tropical cloud forest.
Auteurs : Melissa H. Morris ; Miguel A. Pérez-Pérez ; Matthew E. Smith ; Caroline S. BledsoeSource :
- Mycorrhiza [ 0940-6360 ] ; 2008.
English descriptors
- KwdEn :
- MESH :
- geographic : Mexico.
- classification : Mycorrhizae.
- isolation & purification : Mycorrhizae.
- microbiology : Meristem, Quercus, Trees.
- physiology : Mycorrhizae.
- Biodiversity, Molecular Sequence Data, Tropical Climate.
Abstract
The ecological importance of ectomycorrhizal (EM) fungi in tropical ecosystems is increasingly recognized, but few studies have used molecular methods to examine EM fungal communities in tropical forests. The diversity and composition of the EM community on Quercus crassifolia in a tropical montane cloud forest in southern Mexico were characterized using DNA sequencing of single root tips. Individual root tips commonly harbored multiple fungal species that resulted in mixed polymerase chain reaction (PCR) products. By cloning and performing gel extractions on mixed PCR samples, we identified two or more EM fungi on 26% of the root tips. When non-EM fungi were considered, this figure increased to 31% of root tips. A total of 44 EM taxa and nine non-EM taxa were detected on roots from 21 soil cores (104 root tips). Taxa in the families Russulaceae, Cortinariaceae, Inocybaceae, and Thelephoraceae were frequent. This is the first study to characterize the belowground EM community in a tropical montane cloud forest.
DOI: 10.1007/s00572-008-0186-1
PubMed: 18704515
Links to Exploration step
pubmed:18704515Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Multiple species of ectomycorrhizal fungi are frequently detected on individual oak root tips in a tropical cloud forest.</title><author><name sortKey="Morris, Melissa H" sort="Morris, Melissa H" uniqKey="Morris M" first="Melissa H" last="Morris">Melissa H. Morris</name><affiliation><nlm:affiliation>Department of Land, Air and Water Resources, University of California, Davis, CA, 95616, USA. mhmorris@ucdavis.edu.</nlm:affiliation></affiliation></author><author><name sortKey="Perez Perez, Miguel A" sort="Perez Perez, Miguel A" uniqKey="Perez Perez M" first="Miguel A" last="Pérez-Pérez">Miguel A. Pérez-Pérez</name><affiliation><nlm:affiliation>Centro de Investigaciones en Ecosistemas, Universidad Nacional Autónoma de México, Morelia, 58190, Michoacán, México.</nlm:affiliation></affiliation></author><author><name sortKey="Smith, Matthew E" sort="Smith, Matthew E" uniqKey="Smith M" first="Matthew E" last="Smith">Matthew E. Smith</name><affiliation><nlm:affiliation>Department of Organismic and Evolutionary Biology and the Farlow Herbarium, Harvard University, Cambridge, MA, 02138, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Bledsoe, Caroline S" sort="Bledsoe, Caroline S" uniqKey="Bledsoe C" first="Caroline S" last="Bledsoe">Caroline S. Bledsoe</name><affiliation><nlm:affiliation>Department of Land, Air and Water Resources, University of California, Davis, CA, 95616, USA.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18704515</idno><idno type="pmid">18704515</idno><idno type="doi">10.1007/s00572-008-0186-1</idno><idno type="wicri:Area/Main/Corpus">002C03</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002C03</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Multiple species of ectomycorrhizal fungi are frequently detected on individual oak root tips in a tropical cloud forest.</title><author><name sortKey="Morris, Melissa H" sort="Morris, Melissa H" uniqKey="Morris M" first="Melissa H" last="Morris">Melissa H. Morris</name><affiliation><nlm:affiliation>Department of Land, Air and Water Resources, University of California, Davis, CA, 95616, USA. mhmorris@ucdavis.edu.</nlm:affiliation></affiliation></author><author><name sortKey="Perez Perez, Miguel A" sort="Perez Perez, Miguel A" uniqKey="Perez Perez M" first="Miguel A" last="Pérez-Pérez">Miguel A. Pérez-Pérez</name><affiliation><nlm:affiliation>Centro de Investigaciones en Ecosistemas, Universidad Nacional Autónoma de México, Morelia, 58190, Michoacán, México.</nlm:affiliation></affiliation></author><author><name sortKey="Smith, Matthew E" sort="Smith, Matthew E" uniqKey="Smith M" first="Matthew E" last="Smith">Matthew E. Smith</name><affiliation><nlm:affiliation>Department of Organismic and Evolutionary Biology and the Farlow Herbarium, Harvard University, Cambridge, MA, 02138, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Bledsoe, Caroline S" sort="Bledsoe, Caroline S" uniqKey="Bledsoe C" first="Caroline S" last="Bledsoe">Caroline S. Bledsoe</name><affiliation><nlm:affiliation>Department of Land, Air and Water Resources, University of California, Davis, CA, 95616, USA.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Mycorrhiza</title><idno type="ISSN">0940-6360</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodiversity (MeSH)</term><term>Meristem (microbiology)</term><term>Mexico (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (classification)</term><term>Mycorrhizae (isolation & purification)</term><term>Mycorrhizae (physiology)</term><term>Quercus (microbiology)</term><term>Trees (microbiology)</term><term>Tropical Climate (MeSH)</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Mexico</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Meristem</term><term>Quercus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodiversity</term><term>Molecular Sequence Data</term><term>Tropical Climate</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The ecological importance of ectomycorrhizal (EM) fungi in tropical ecosystems is increasingly recognized, but few studies have used molecular methods to examine EM fungal communities in tropical forests. The diversity and composition of the EM community on Quercus crassifolia in a tropical montane cloud forest in southern Mexico were characterized using DNA sequencing of single root tips. Individual root tips commonly harbored multiple fungal species that resulted in mixed polymerase chain reaction (PCR) products. By cloning and performing gel extractions on mixed PCR samples, we identified two or more EM fungi on 26% of the root tips. When non-EM fungi were considered, this figure increased to 31% of root tips. A total of 44 EM taxa and nine non-EM taxa were detected on roots from 21 soil cores (104 root tips). Taxa in the families Russulaceae, Cortinariaceae, Inocybaceae, and Thelephoraceae were frequent. This is the first study to characterize the belowground EM community in a tropical montane cloud forest.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18704515</PMID><DateCompleted><Year>2009</Year><Month>01</Month><Day>15</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0940-6360</ISSN><JournalIssue CitedMedium="Print"><Volume>18</Volume><Issue>8</Issue><PubDate><Year>2008</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>Multiple species of ectomycorrhizal fungi are frequently detected on individual oak root tips in a tropical cloud forest.</ArticleTitle><Pagination><MedlinePgn>375-383</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00572-008-0186-1</ELocationID><Abstract><AbstractText>The ecological importance of ectomycorrhizal (EM) fungi in tropical ecosystems is increasingly recognized, but few studies have used molecular methods to examine EM fungal communities in tropical forests. The diversity and composition of the EM community on Quercus crassifolia in a tropical montane cloud forest in southern Mexico were characterized using DNA sequencing of single root tips. Individual root tips commonly harbored multiple fungal species that resulted in mixed polymerase chain reaction (PCR) products. By cloning and performing gel extractions on mixed PCR samples, we identified two or more EM fungi on 26% of the root tips. When non-EM fungi were considered, this figure increased to 31% of root tips. A total of 44 EM taxa and nine non-EM taxa were detected on roots from 21 soil cores (104 root tips). Taxa in the families Russulaceae, Cortinariaceae, Inocybaceae, and Thelephoraceae were frequent. This is the first study to characterize the belowground EM community in a tropical montane cloud forest.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Morris</LastName><ForeName>Melissa H</ForeName><Initials>MH</Initials><AffiliationInfo><Affiliation>Department of Land, Air and Water Resources, University of California, Davis, CA, 95616, USA. mhmorris@ucdavis.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pérez-Pérez</LastName><ForeName>Miguel A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Centro de Investigaciones en Ecosistemas, Universidad Nacional Autónoma de México, Morelia, 58190, Michoacán, México.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Smith</LastName><ForeName>Matthew E</ForeName><Initials>ME</Initials><AffiliationInfo><Affiliation>Department of Organismic and Evolutionary Biology and the Farlow Herbarium, Harvard University, Cambridge, MA, 02138, 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