The ftsZ gene of the endocellular bacterium 'Candidatus Glomeribacter gigasporarum' is preferentially expressed during the symbiotic phases of its host mycorrhizal fungus.
Identifieur interne : 002A34 ( Main/Corpus ); précédent : 002A33; suivant : 002A35The ftsZ gene of the endocellular bacterium 'Candidatus Glomeribacter gigasporarum' is preferentially expressed during the symbiotic phases of its host mycorrhizal fungus.
Auteurs : Iulia-Andra Anca ; Erica Lumini ; Stefano Ghignone ; Alessandra Salvioli ; Valeria Bianciotto ; Paola BonfanteSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2009.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Base Sequence (MeSH), Burkholderiaceae (genetics), Burkholderiaceae (metabolism), Cytoskeletal Proteins (genetics), Cytoskeletal Proteins (metabolism), Gene Expression Regulation, Bacterial (MeSH), Molecular Sequence Data (MeSH), Mycorrhizae (physiology), Phylogeny (MeSH), Symbiosis (physiology).
- MESH :
- chemical , genetics : Bacterial Proteins, Cytoskeletal Proteins.
- chemical , metabolism : Bacterial Proteins, Cytoskeletal Proteins.
- genetics : Burkholderiaceae.
- metabolism : Burkholderiaceae.
- physiology : Mycorrhizae, Symbiosis.
- Amino Acid Sequence, Base Sequence, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Phylogeny.
Abstract
The arbuscular mycorrhizal fungus (AM) Gigaspora margarita consistently hosts bacteria, named 'Candidatus Glomeribacter gigasporarum,' inside its cytoplasm. Endobacteria have a positive impact on fungal fitness during the presymbiotic phase, prior to plant roots colonization. We tested the hypothesis that the endobacterium and its cell divisions depend on fungal metabolism, mirroring also the events of the fungal life cycle which are influenced by plant signals. We first cloned a fragment of ftsZ, a marker gene for bacterial division, and then analyzed its expression along the different stages of fungus development. The bacterial gene transcripts showed the highest values when the fungus was associated to the plant, and peaked in the extraradical mycelium. Strigolactones, which are known to stimulate the AM fungal growth, caused a significant transcript increase in the germinated spores in the absence of the plant. The quantitative real-time reverse-transcription polymerase chain reaction data were strengthened by the quantification of the dividing bacteria, which were increasing in number in germinating spores after the strigolactone treatment. The bioactive molecule alone did not cause any change in the number of bacteria after their isolation from the fungus, thus showing that the strigolactone alone cannot confer free-living capacities to the bacterium.
DOI: 10.1094/MPMI-22-3-0302
PubMed: 19245324
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pubmed:19245324Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The ftsZ gene of the endocellular bacterium 'Candidatus Glomeribacter gigasporarum' is preferentially expressed during the symbiotic phases of its host mycorrhizal fungus.</title><author><name sortKey="Anca, Iulia Andra" sort="Anca, Iulia Andra" uniqKey="Anca I" first="Iulia-Andra" last="Anca">Iulia-Andra Anca</name><affiliation><nlm:affiliation>Università degli Studi di Torino, Dip. Biologia Vegetale, V. le P.A. Mattioli 25, I-10125, Torino, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Lumini, Erica" sort="Lumini, Erica" uniqKey="Lumini E" first="Erica" last="Lumini">Erica Lumini</name></author><author><name sortKey="Ghignone, Stefano" sort="Ghignone, Stefano" uniqKey="Ghignone S" first="Stefano" last="Ghignone">Stefano Ghignone</name></author><author><name sortKey="Salvioli, Alessandra" sort="Salvioli, Alessandra" uniqKey="Salvioli A" first="Alessandra" last="Salvioli">Alessandra Salvioli</name></author><author><name sortKey="Bianciotto, Valeria" sort="Bianciotto, Valeria" uniqKey="Bianciotto V" first="Valeria" last="Bianciotto">Valeria Bianciotto</name></author><author><name sortKey="Bonfante, Paola" sort="Bonfante, Paola" uniqKey="Bonfante P" first="Paola" last="Bonfante">Paola Bonfante</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19245324</idno><idno type="pmid">19245324</idno><idno type="doi">10.1094/MPMI-22-3-0302</idno><idno type="wicri:Area/Main/Corpus">002A34</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002A34</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The ftsZ gene of the endocellular bacterium 'Candidatus Glomeribacter gigasporarum' is preferentially expressed during the symbiotic phases of its host mycorrhizal fungus.</title><author><name sortKey="Anca, Iulia Andra" sort="Anca, Iulia Andra" uniqKey="Anca I" first="Iulia-Andra" last="Anca">Iulia-Andra Anca</name><affiliation><nlm:affiliation>Università degli Studi di Torino, Dip. Biologia Vegetale, V. le P.A. Mattioli 25, I-10125, Torino, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Lumini, Erica" sort="Lumini, Erica" uniqKey="Lumini E" first="Erica" last="Lumini">Erica Lumini</name></author><author><name sortKey="Ghignone, Stefano" sort="Ghignone, Stefano" uniqKey="Ghignone S" first="Stefano" last="Ghignone">Stefano Ghignone</name></author><author><name sortKey="Salvioli, Alessandra" sort="Salvioli, Alessandra" uniqKey="Salvioli A" first="Alessandra" last="Salvioli">Alessandra Salvioli</name></author><author><name sortKey="Bianciotto, Valeria" sort="Bianciotto, Valeria" uniqKey="Bianciotto V" first="Valeria" last="Bianciotto">Valeria Bianciotto</name></author><author><name sortKey="Bonfante, Paola" sort="Bonfante, Paola" uniqKey="Bonfante P" first="Paola" last="Bonfante">Paola Bonfante</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (metabolism)</term><term>Base Sequence (MeSH)</term><term>Burkholderiaceae (genetics)</term><term>Burkholderiaceae (metabolism)</term><term>Cytoskeletal Proteins (genetics)</term><term>Cytoskeletal Proteins (metabolism)</term><term>Gene Expression Regulation, Bacterial (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (physiology)</term><term>Phylogeny (MeSH)</term><term>Symbiosis (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Cytoskeletal Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term><term>Cytoskeletal Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Burkholderiaceae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Burkholderiaceae</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mycorrhizae</term><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Gene Expression Regulation, Bacterial</term><term>Molecular Sequence Data</term><term>Phylogeny</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The arbuscular mycorrhizal fungus (AM) Gigaspora margarita consistently hosts bacteria, named 'Candidatus Glomeribacter gigasporarum,' inside its cytoplasm. Endobacteria have a positive impact on fungal fitness during the presymbiotic phase, prior to plant roots colonization. We tested the hypothesis that the endobacterium and its cell divisions depend on fungal metabolism, mirroring also the events of the fungal life cycle which are influenced by plant signals. We first cloned a fragment of ftsZ, a marker gene for bacterial division, and then analyzed its expression along the different stages of fungus development. The bacterial gene transcripts showed the highest values when the fungus was associated to the plant, and peaked in the extraradical mycelium. Strigolactones, which are known to stimulate the AM fungal growth, caused a significant transcript increase in the germinated spores in the absence of the plant. The quantitative real-time reverse-transcription polymerase chain reaction data were strengthened by the quantification of the dividing bacteria, which were increasing in number in germinating spores after the strigolactone treatment. The bioactive molecule alone did not cause any change in the number of bacteria after their isolation from the fungus, thus showing that the strigolactone alone cannot confer free-living capacities to the bacterium.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19245324</PMID><DateCompleted><Year>2009</Year><Month>07</Month><Day>01</Day></DateCompleted><DateRevised><Year>2009</Year><Month>02</Month><Day>27</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>22</Volume><Issue>3</Issue><PubDate><Year>2009</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>The ftsZ gene of the endocellular bacterium 'Candidatus Glomeribacter gigasporarum' is preferentially expressed during the symbiotic phases of its host mycorrhizal fungus.</ArticleTitle><Pagination><MedlinePgn>302-10</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-22-3-0302</ELocationID><Abstract><AbstractText>The arbuscular mycorrhizal fungus (AM) Gigaspora margarita consistently hosts bacteria, named 'Candidatus Glomeribacter gigasporarum,' inside its cytoplasm. Endobacteria have a positive impact on fungal fitness during the presymbiotic phase, prior to plant roots colonization. We tested the hypothesis that the endobacterium and its cell divisions depend on fungal metabolism, mirroring also the events of the fungal life cycle which are influenced by plant signals. We first cloned a fragment of ftsZ, a marker gene for bacterial division, and then analyzed its expression along the different stages of fungus development. The bacterial gene transcripts showed the highest values when the fungus was associated to the plant, and peaked in the extraradical mycelium. Strigolactones, which are known to stimulate the AM fungal growth, caused a significant transcript increase in the germinated spores in the absence of the plant. The quantitative real-time reverse-transcription polymerase chain reaction data were strengthened by the quantification of the dividing bacteria, which were increasing in number in germinating spores after the strigolactone treatment. The bioactive molecule alone did not cause any change in the number of bacteria after their isolation from the fungus, thus showing that the strigolactone alone cannot confer free-living capacities to the bacterium.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Anca</LastName><ForeName>Iulia-Andra</ForeName><Initials>IA</Initials><AffiliationInfo><Affiliation>Università degli Studi di Torino, Dip. Biologia Vegetale, V. le P.A. Mattioli 25, I-10125, Torino, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lumini</LastName><ForeName>Erica</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Ghignone</LastName><ForeName>Stefano</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Salvioli</LastName><ForeName>Alessandra</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Bianciotto</LastName><ForeName>Valeria</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Bonfante</LastName><ForeName>Paola</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003598">Cytoskeletal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C071525">FtsZ protein, Bacteria</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042521" MajorTopicYN="N">Burkholderiaceae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003598" MajorTopicYN="N">Cytoskeletal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015964" MajorTopicYN="N">Gene Expression Regulation, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>2</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>2</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>7</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19245324</ArticleId><ArticleId IdType="doi">10.1094/MPMI-22-3-0302</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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