Functional characterization of a C-4 sterol methyl oxidase from the endomycorrhizal fungus Glomus intraradices.
Identifieur interne : 002A21 ( Main/Corpus ); précédent : 002A20; suivant : 002A22Functional characterization of a C-4 sterol methyl oxidase from the endomycorrhizal fungus Glomus intraradices.
Auteurs : Elodie Oger ; Stefano Ghignone ; Estelle Campagnac ; Joël Fontaine ; Anne Grandmougin-Ferjani ; Luisa LanfrancoSource :
- Fungal genetics and biology : FG & B [ 1096-0937 ]
English descriptors
- KwdEn :
- Amino Acid Motifs (MeSH), Amino Acid Sequence (MeSH), Fungal Proteins (chemistry), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression Regulation, Enzymologic (MeSH), Glomeromycota (chemistry), Glomeromycota (classification), Glomeromycota (enzymology), Glomeromycota (genetics), Mixed Function Oxygenases (chemistry), Mixed Function Oxygenases (genetics), Mixed Function Oxygenases (metabolism), Molecular Sequence Data (MeSH), Molecular Structure (MeSH), Mycorrhizae (chemistry), Mycorrhizae (classification), Mycorrhizae (enzymology), Mycorrhizae (genetics), Phylogeny (MeSH), Sequence Alignment (MeSH), Sterols (metabolism).
- MESH :
- chemical , chemistry : Fungal Proteins, Mixed Function Oxygenases.
- chemical , genetics : Fungal Proteins, Mixed Function Oxygenases.
- chemical , metabolism : Fungal Proteins, Mixed Function Oxygenases, Sterols.
- chemistry : Glomeromycota, Mycorrhizae.
- classification : Glomeromycota, Mycorrhizae.
- enzymology : Glomeromycota, Mycorrhizae.
- genetics : Glomeromycota, Mycorrhizae.
- Amino Acid Motifs, Amino Acid Sequence, Gene Expression Regulation, Enzymologic, Molecular Sequence Data, Molecular Structure, Phylogeny, Sequence Alignment.
Abstract
Sterols are crucial components of eukaryotic membranes that control membrane fluidity and permeability. They play an important role in cell signaling, polarity and sorting. Since many steps in the pathway are essential, sterol biosynthesis inhibitors (SBI) are widely used as antifungal agents. This work reports the identification and the characterization of a C-4 sterol methyl oxidase (SMO), the first gene involved in the sterol biosynthetic pathway, so far described from an arbuscular mycorrhizal fungus. The sequence, called GintSMO, shows a primary structure, a hydrophobicity profile and a pattern of histidine-rich motifs which are typical of C-4 methyl sterol oxidases. The complementation assay in a Saccharomyces cerevisiae mutant strain demonstrates that GintSMO encodes a functional SMO. Changes in GintSMO transcript levels and in the amount of the sterol precursor squalene were observed in in vitro grown extraradical structures exposed to the fenpropimorph SBI fungicide.
DOI: 10.1016/j.fgb.2009.03.002
PubMed: 19285148
Links to Exploration step
pubmed:19285148Le document en format XML
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<author><name sortKey="Ghignone, Stefano" sort="Ghignone, Stefano" uniqKey="Ghignone S" first="Stefano" last="Ghignone">Stefano Ghignone</name>
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<author><name sortKey="Campagnac, Estelle" sort="Campagnac, Estelle" uniqKey="Campagnac E" first="Estelle" last="Campagnac">Estelle Campagnac</name>
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<author><name sortKey="Fontaine, Joel" sort="Fontaine, Joel" uniqKey="Fontaine J" first="Joël" last="Fontaine">Joël Fontaine</name>
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<author><name sortKey="Grandmougin Ferjani, Anne" sort="Grandmougin Ferjani, Anne" uniqKey="Grandmougin Ferjani A" first="Anne" last="Grandmougin-Ferjani">Anne Grandmougin-Ferjani</name>
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<author><name sortKey="Lanfranco, Luisa" sort="Lanfranco, Luisa" uniqKey="Lanfranco L" first="Luisa" last="Lanfranco">Luisa Lanfranco</name>
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<author><name sortKey="Grandmougin Ferjani, Anne" sort="Grandmougin Ferjani, Anne" uniqKey="Grandmougin Ferjani A" first="Anne" last="Grandmougin-Ferjani">Anne Grandmougin-Ferjani</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs (MeSH)</term>
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<term>Fungal Proteins (genetics)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Gene Expression Regulation, Enzymologic (MeSH)</term>
<term>Glomeromycota (chemistry)</term>
<term>Glomeromycota (classification)</term>
<term>Glomeromycota (enzymology)</term>
<term>Glomeromycota (genetics)</term>
<term>Mixed Function Oxygenases (chemistry)</term>
<term>Mixed Function Oxygenases (genetics)</term>
<term>Mixed Function Oxygenases (metabolism)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Molecular Structure (MeSH)</term>
<term>Mycorrhizae (chemistry)</term>
<term>Mycorrhizae (classification)</term>
<term>Mycorrhizae (enzymology)</term>
<term>Mycorrhizae (genetics)</term>
<term>Phylogeny (MeSH)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sterols (metabolism)</term>
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<term>Mixed Function Oxygenases</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term>
<term>Mixed Function Oxygenases</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term>
<term>Mixed Function Oxygenases</term>
<term>Sterols</term>
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<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Glomeromycota</term>
<term>Mycorrhizae</term>
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<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Glomeromycota</term>
<term>Mycorrhizae</term>
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<term>Mycorrhizae</term>
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<term>Amino Acid Sequence</term>
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<front><div type="abstract" xml:lang="en">Sterols are crucial components of eukaryotic membranes that control membrane fluidity and permeability. They play an important role in cell signaling, polarity and sorting. Since many steps in the pathway are essential, sterol biosynthesis inhibitors (SBI) are widely used as antifungal agents. This work reports the identification and the characterization of a C-4 sterol methyl oxidase (SMO), the first gene involved in the sterol biosynthetic pathway, so far described from an arbuscular mycorrhizal fungus. The sequence, called GintSMO, shows a primary structure, a hydrophobicity profile and a pattern of histidine-rich motifs which are typical of C-4 methyl sterol oxidases. The complementation assay in a Saccharomyces cerevisiae mutant strain demonstrates that GintSMO encodes a functional SMO. Changes in GintSMO transcript levels and in the amount of the sterol precursor squalene were observed in in vitro grown extraradical structures exposed to the fenpropimorph SBI fungicide.</div>
</front>
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<Title>Fungal genetics and biology : FG & B</Title>
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<ArticleTitle>Functional characterization of a C-4 sterol methyl oxidase from the endomycorrhizal fungus Glomus intraradices.</ArticleTitle>
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<Abstract><AbstractText>Sterols are crucial components of eukaryotic membranes that control membrane fluidity and permeability. They play an important role in cell signaling, polarity and sorting. Since many steps in the pathway are essential, sterol biosynthesis inhibitors (SBI) are widely used as antifungal agents. This work reports the identification and the characterization of a C-4 sterol methyl oxidase (SMO), the first gene involved in the sterol biosynthetic pathway, so far described from an arbuscular mycorrhizal fungus. The sequence, called GintSMO, shows a primary structure, a hydrophobicity profile and a pattern of histidine-rich motifs which are typical of C-4 methyl sterol oxidases. The complementation assay in a Saccharomyces cerevisiae mutant strain demonstrates that GintSMO encodes a functional SMO. Changes in GintSMO transcript levels and in the amount of the sterol precursor squalene were observed in in vitro grown extraradical structures exposed to the fenpropimorph SBI fungicide.</AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Oger</LastName>
<ForeName>Elodie</ForeName>
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