Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype.
Identifieur interne : 002726 ( Main/Corpus ); précédent : 002725; suivant : 002727Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype.
Auteurs : Philippe Jourand ; Marc Ducousso ; Clarisse Loulergue-Majorel ; Laure Hannibal ; Sylvain Santoni ; Yves Prin ; Michel LebrunSource :
- FEMS microbiology ecology [ 1574-6941 ] ; 2010.
English descriptors
- KwdEn :
- Amplified Fragment Length Polymorphism Analysis (MeSH), DNA, Fungal (genetics), DNA, Ribosomal Spacer (genetics), Genetic Variation (MeSH), Mycelium (metabolism), Mycorrhizae (genetics), Mycorrhizae (isolation & purification), New Caledonia (MeSH), Nickel (metabolism), Phenotype (MeSH), Phylogeny (MeSH), Polymorphism, Restriction Fragment Length (MeSH), Sequence Analysis, DNA (MeSH), Soil (analysis), Soil Microbiology (MeSH).
- MESH :
- chemical , analysis : Soil.
- chemical , genetics : DNA, Fungal, DNA, Ribosomal Spacer.
- genetics : Mycorrhizae.
- isolation & purification : Mycorrhizae.
- metabolism : Mycelium, Nickel.
- Amplified Fragment Length Polymorphism Analysis, Genetic Variation, New Caledonia, Phenotype, Phylogeny, Polymorphism, Restriction Fragment Length, Sequence Analysis, DNA, Soil Microbiology.
Abstract
Isolates of ectomycorrhizal Pisolithus albus were sampled from both ultramafic and volcano-sedimentary soils in New Caledonia, a tropical hotspot of biodiversity, to investigate the relationships between genetic diversity and edaphic constraint through tolerance to nickel (Ni). Carpophore description, spore morphology and phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequences confirmed that all isolates belong to P. albus and are closely related to other Australasian specimens. Using molecular tools, ITS-restriction fragment length polymorphism and amplified fragment length polymorphism markers, we showed the existence of two distinct genetic clusters within P. albus: ultramafic and volcano-sedimentary. Mycelia response to Ni toxicity supports such a population structure. Pisolithus albus from ultramafic soils included isolates with a high diversity of in vitro Ni tolerance, with both Ni-tolerant isolates (average Ni EC(50) at 575 microM) and Ni-sensitive isolates (average Ni EC(50) at 37 microM). In contrast, all isolates from volcano-sedimentary soils were found to be Ni sensitive (average Ni EC(50) at 32 microM). We highlight that (1) P. albus population from ultramafic soils of New Caledonia are genetically structured in ecotype, and that (2) Ni tolerance among ultramafic isolates suggests an adaptive physiological response to Ni toxicity.
DOI: 10.1111/j.1574-6941.2010.00843.x
PubMed: 20199570
Links to Exploration step
pubmed:20199570Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype.</title><author><name sortKey="Jourand, Philippe" sort="Jourand, Philippe" uniqKey="Jourand P" first="Philippe" last="Jourand">Philippe Jourand</name><affiliation><nlm:affiliation>Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), UMR 113 CIRAD/INRA/IRD/SupAgro/UMII, Centre IRD, Nouméa, Nouvelle-Calédonie. philippe.jourand@ird.fr</nlm:affiliation></affiliation></author><author><name sortKey="Ducousso, Marc" sort="Ducousso, Marc" uniqKey="Ducousso M" first="Marc" last="Ducousso">Marc Ducousso</name></author><author><name sortKey="Loulergue Majorel, Clarisse" sort="Loulergue Majorel, Clarisse" uniqKey="Loulergue Majorel C" first="Clarisse" last="Loulergue-Majorel">Clarisse Loulergue-Majorel</name></author><author><name sortKey="Hannibal, Laure" sort="Hannibal, Laure" uniqKey="Hannibal L" first="Laure" last="Hannibal">Laure Hannibal</name></author><author><name sortKey="Santoni, Sylvain" sort="Santoni, Sylvain" uniqKey="Santoni S" first="Sylvain" last="Santoni">Sylvain Santoni</name></author><author><name sortKey="Prin, Yves" sort="Prin, Yves" uniqKey="Prin Y" first="Yves" last="Prin">Yves Prin</name></author><author><name sortKey="Lebrun, Michel" sort="Lebrun, Michel" uniqKey="Lebrun M" first="Michel" last="Lebrun">Michel Lebrun</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20199570</idno><idno type="pmid">20199570</idno><idno type="doi">10.1111/j.1574-6941.2010.00843.x</idno><idno type="wicri:Area/Main/Corpus">002726</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002726</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype.</title><author><name sortKey="Jourand, Philippe" sort="Jourand, Philippe" uniqKey="Jourand P" first="Philippe" last="Jourand">Philippe Jourand</name><affiliation><nlm:affiliation>Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), UMR 113 CIRAD/INRA/IRD/SupAgro/UMII, Centre IRD, Nouméa, Nouvelle-Calédonie. philippe.jourand@ird.fr</nlm:affiliation></affiliation></author><author><name sortKey="Ducousso, Marc" sort="Ducousso, Marc" uniqKey="Ducousso M" first="Marc" last="Ducousso">Marc Ducousso</name></author><author><name sortKey="Loulergue Majorel, Clarisse" sort="Loulergue Majorel, Clarisse" uniqKey="Loulergue Majorel C" first="Clarisse" last="Loulergue-Majorel">Clarisse Loulergue-Majorel</name></author><author><name sortKey="Hannibal, Laure" sort="Hannibal, Laure" uniqKey="Hannibal L" first="Laure" last="Hannibal">Laure Hannibal</name></author><author><name sortKey="Santoni, Sylvain" sort="Santoni, Sylvain" uniqKey="Santoni S" first="Sylvain" last="Santoni">Sylvain Santoni</name></author><author><name sortKey="Prin, Yves" sort="Prin, Yves" uniqKey="Prin Y" first="Yves" last="Prin">Yves Prin</name></author><author><name sortKey="Lebrun, Michel" sort="Lebrun, Michel" uniqKey="Lebrun M" first="Michel" last="Lebrun">Michel Lebrun</name></author></analytic><series><title level="j">FEMS microbiology ecology</title><idno type="eISSN">1574-6941</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amplified Fragment Length Polymorphism Analysis (MeSH)</term><term>DNA, Fungal (genetics)</term><term>DNA, Ribosomal Spacer (genetics)</term><term>Genetic Variation (MeSH)</term><term>Mycelium (metabolism)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (isolation & purification)</term><term>New Caledonia (MeSH)</term><term>Nickel (metabolism)</term><term>Phenotype (MeSH)</term><term>Phylogeny (MeSH)</term><term>Polymorphism, Restriction Fragment Length (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Soil (analysis)</term><term>Soil Microbiology (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Soil</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Fungal</term><term>DNA, Ribosomal Spacer</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Mycelium</term><term>Nickel</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amplified Fragment Length Polymorphism Analysis</term><term>Genetic Variation</term><term>New Caledonia</term><term>Phenotype</term><term>Phylogeny</term><term>Polymorphism, Restriction Fragment Length</term><term>Sequence Analysis, DNA</term><term>Soil Microbiology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Isolates of ectomycorrhizal Pisolithus albus were sampled from both ultramafic and volcano-sedimentary soils in New Caledonia, a tropical hotspot of biodiversity, to investigate the relationships between genetic diversity and edaphic constraint through tolerance to nickel (Ni). Carpophore description, spore morphology and phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequences confirmed that all isolates belong to P. albus and are closely related to other Australasian specimens. Using molecular tools, ITS-restriction fragment length polymorphism and amplified fragment length polymorphism markers, we showed the existence of two distinct genetic clusters within P. albus: ultramafic and volcano-sedimentary. Mycelia response to Ni toxicity supports such a population structure. Pisolithus albus from ultramafic soils included isolates with a high diversity of in vitro Ni tolerance, with both Ni-tolerant isolates (average Ni EC(50) at 575 microM) and Ni-sensitive isolates (average Ni EC(50) at 37 microM). In contrast, all isolates from volcano-sedimentary soils were found to be Ni sensitive (average Ni EC(50) at 32 microM). We highlight that (1) P. albus population from ultramafic soils of New Caledonia are genetically structured in ecotype, and that (2) Ni tolerance among ultramafic isolates suggests an adaptive physiological response to Ni toxicity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20199570</PMID><DateCompleted><Year>2010</Year><Month>09</Month><Day>08</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1574-6941</ISSN><JournalIssue CitedMedium="Internet"><Volume>72</Volume><Issue>2</Issue><PubDate><Year>2010</Year><Month>May</Month></PubDate></JournalIssue><Title>FEMS microbiology ecology</Title><ISOAbbreviation>FEMS Microbiol Ecol</ISOAbbreviation></Journal><ArticleTitle>Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype.</ArticleTitle><Pagination><MedlinePgn>238-49</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1574-6941.2010.00843.x</ELocationID><Abstract><AbstractText>Isolates of ectomycorrhizal Pisolithus albus were sampled from both ultramafic and volcano-sedimentary soils in New Caledonia, a tropical hotspot of biodiversity, to investigate the relationships between genetic diversity and edaphic constraint through tolerance to nickel (Ni). Carpophore description, spore morphology and phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequences confirmed that all isolates belong to P. albus and are closely related to other Australasian specimens. Using molecular tools, ITS-restriction fragment length polymorphism and amplified fragment length polymorphism markers, we showed the existence of two distinct genetic clusters within P. albus: ultramafic and volcano-sedimentary. Mycelia response to Ni toxicity supports such a population structure. Pisolithus albus from ultramafic soils included isolates with a high diversity of in vitro Ni tolerance, with both Ni-tolerant isolates (average Ni EC(50) at 575 microM) and Ni-sensitive isolates (average Ni EC(50) at 37 microM). In contrast, all isolates from volcano-sedimentary soils were found to be Ni sensitive (average Ni EC(50) at 32 microM). We highlight that (1) P. albus population from ultramafic soils of New Caledonia are genetically structured in ecotype, and that (2) Ni tolerance among ultramafic isolates suggests an adaptive physiological response to Ni toxicity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jourand</LastName><ForeName>Philippe</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), UMR 113 CIRAD/INRA/IRD/SupAgro/UMII, Centre IRD, Nouméa, Nouvelle-Calédonie. philippe.jourand@ird.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ducousso</LastName><ForeName>Marc</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Loulergue-Majorel</LastName><ForeName>Clarisse</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Hannibal</LastName><ForeName>Laure</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Santoni</LastName><ForeName>Sylvain</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Prin</LastName><ForeName>Yves</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Lebrun</LastName><ForeName>Michel</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>01</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEMS Microbiol Ecol</MedlineTA><NlmUniqueID>8901229</NlmUniqueID><ISSNLinking>0168-6496</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021903">DNA, Ribosomal Spacer</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012987">Soil</NameOfSubstance></Chemical><Chemical><RegistryNumber>7OV03QG267</RegistryNumber><NameOfSubstance UI="D009532">Nickel</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D054458" MajorTopicYN="N">Amplified Fragment Length Polymorphism Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021903" MajorTopicYN="N">DNA, Ribosomal Spacer</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="N">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D025282" MajorTopicYN="N">Mycelium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009510" MajorTopicYN="N">New Caledonia</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009532" MajorTopicYN="N">Nickel</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012150" MajorTopicYN="N">Polymorphism, Restriction Fragment Length</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012987" MajorTopicYN="N">Soil</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil Microbiology</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>9</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20199570</ArticleId><ArticleId IdType="pii">FEM843</ArticleId><ArticleId IdType="doi">10.1111/j.1574-6941.2010.00843.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MycorrhizaeV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002726 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 002726 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MycorrhizaeV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:20199570
|texte= Ultramafic soils from New Caledonia structure Pisolithus albus in ecotype.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:20199570" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a MycorrhizaeV1
| This area was generated with Dilib version V0.6.37. |  |