Carbon allocation in ectomycorrhizal plants at limited optimal N supply: an attempt aat unraveling conflicting theories.
Identifieur interne : 002694 ( Main/Corpus ); précédent : 002693; suivant : 002695Carbon allocation in ectomycorrhizal plants at limited optimal N supply: an attempt aat unraveling conflicting theories.
Auteurs : Ana Corrêa ; Rüdiger Hampp ; Elisabeth Magel ; Maria-Amélia Martins-LouçãoSource :
- Mycorrhiza [ 1432-1890 ] ; 2011.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Carbon, Nitrogen.
- growth & development : Basidiomycota, Mycorrhizae.
- metabolism : Basidiomycota, Mycorrhizae, Pinus, Plant Roots.
- microbiology : Pinus, Plant Roots.
Abstract
With regard to mycorrhiza, conflicting theories try to explain how the balance between fungal demand for carbohydrates and the plant’s needs for nutrients varies, resulting in conflicting predictions. In order to evaluate current concepts, we investigated some metabolic parameters, which are indicative for plant carbon allocation in response to mycorrhization at limited and optimal N supply. Pinus pinaster seedlings were inoculated with living or dead (control) cultures of Pisolithus tinctorius, supplied with ammonium at 4 (limiting) or 7% d−1 (non-limiting) N relative addition rate (RARN), and followed development for 29 days. Mycorrhizal colonization of roots was quantified by the determination of ergosterol. A series of enzymes (sucrose and trehalose metabolism, anaplerosis) and metabolites (soluble carbohydrate, including trehalose; fructose 2,6 bisphosphate, free amino acids) relevant in the C/N exchange between symbionts, and in the carbon allocation and sink strength within the plant were assayed for 2-day-intervals for up to 14 days, and at 5-day-intervals for the rest of the experiment. The first 10 days reflected the establishment of mycorrhizal interaction, and the carbon allocation to the root was higher in M plants independent of N supply. Following this period, carbon allocation became N-related, higher at low, and lower at high N supply. The belowground C investment of M plants was dependent on N availability, but not on N gain. Finally, increased belowground C allocation was accompanied by a shift from plant to fungal metabolism.
DOI: 10.1007/s00572-010-0309-3
PubMed: 20393757
Links to Exploration step
pubmed:20393757Le document en format XML
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Piso 4, 1749-016 Lisbon, Portugal. ambcorrea@gmail.com</nlm:affiliation></affiliation></author><author><name sortKey="Hampp, Rudiger" sort="Hampp, Rudiger" uniqKey="Hampp R" first="Rüdiger" last="Hampp">Rüdiger Hampp</name></author><author><name sortKey="Magel, Elisabeth" sort="Magel, Elisabeth" uniqKey="Magel E" first="Elisabeth" last="Magel">Elisabeth Magel</name></author><author><name sortKey="Martins Loucao, Maria Amelia" sort="Martins Loucao, Maria Amelia" uniqKey="Martins Loucao M" first="Maria-Amélia" last="Martins-Loução">Maria-Amélia Martins-Loução</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:20393757</idno><idno type="pmid">20393757</idno><idno type="doi">10.1007/s00572-010-0309-3</idno><idno type="wicri:Area/Main/Corpus">002694</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002694</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Carbon allocation in ectomycorrhizal plants at limited optimal N supply: an attempt aat unraveling conflicting theories.</title><author><name sortKey="Correa, Ana" sort="Correa, Ana" uniqKey="Correa A" first="Ana" last="Corrêa">Ana Corrêa</name><affiliation><nlm:affiliation>Universidade de Lisboa, Faculdade de Ciências, Centro de Biologia Aplicada, Campo Grande C2. Piso 4, 1749-016 Lisbon, Portugal. ambcorrea@gmail.com</nlm:affiliation></affiliation></author><author><name sortKey="Hampp, Rudiger" sort="Hampp, Rudiger" uniqKey="Hampp R" first="Rüdiger" last="Hampp">Rüdiger Hampp</name></author><author><name sortKey="Magel, Elisabeth" sort="Magel, Elisabeth" uniqKey="Magel E" first="Elisabeth" last="Magel">Elisabeth Magel</name></author><author><name sortKey="Martins Loucao, Maria Amelia" sort="Martins Loucao, Maria Amelia" uniqKey="Martins Loucao M" first="Maria-Amélia" last="Martins-Loução">Maria-Amélia Martins-Loução</name></author></analytic><series><title level="j">Mycorrhiza</title><idno type="eISSN">1432-1890</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (growth & development)</term><term>Basidiomycota (metabolism)</term><term>Carbon (metabolism)</term><term>Mycorrhizae (growth & development)</term><term>Mycorrhizae (metabolism)</term><term>Nitrogen (metabolism)</term><term>Pinus (metabolism)</term><term>Pinus (microbiology)</term><term>Plant Roots (metabolism)</term><term>Plant Roots (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon</term><term>Nitrogen</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Basidiomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term><term>Mycorrhizae</term><term>Pinus</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Pinus</term><term>Plant Roots</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">With regard to mycorrhiza, conflicting theories try to explain how the balance between fungal demand for carbohydrates and the plant’s needs for nutrients varies, resulting in conflicting predictions. In order to evaluate current concepts, we investigated some metabolic parameters, which are indicative for plant carbon allocation in response to mycorrhization at limited and optimal N supply. Pinus pinaster seedlings were inoculated with living or dead (control) cultures of Pisolithus tinctorius, supplied with ammonium at 4 (limiting) or 7% d−1 (non-limiting) N relative addition rate (RARN), and followed development for 29 days. Mycorrhizal colonization of roots was quantified by the determination of ergosterol. A series of enzymes (sucrose and trehalose metabolism, anaplerosis) and metabolites (soluble carbohydrate, including trehalose; fructose 2,6 bisphosphate, free amino acids) relevant in the C/N exchange between symbionts, and in the carbon allocation and sink strength within the plant were assayed for 2-day-intervals for up to 14 days, and at 5-day-intervals for the rest of the experiment. The first 10 days reflected the establishment of mycorrhizal interaction, and the carbon allocation to the root was higher in M plants independent of N supply. Following this period, carbon allocation became N-related, higher at low, and lower at high N supply. The belowground C investment of M plants was dependent on N availability, but not on N gain. Finally, increased belowground C allocation was accompanied by a shift from plant to fungal metabolism.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20393757</PMID><DateCompleted><Year>2011</Year><Month>04</Month><Day>04</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1432-1890</ISSN><JournalIssue CitedMedium="Internet"><Volume>21</Volume><Issue>1</Issue><PubDate><Year>2011</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>Carbon allocation in ectomycorrhizal plants at limited optimal N supply: an attempt aat unraveling conflicting theories.</ArticleTitle><Pagination><MedlinePgn>35-51</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00572-010-0309-3</ELocationID><Abstract><AbstractText>With regard to mycorrhiza, conflicting theories try to explain how the balance between fungal demand for carbohydrates and the plant’s needs for nutrients varies, resulting in conflicting predictions. In order to evaluate current concepts, we investigated some metabolic parameters, which are indicative for plant carbon allocation in response to mycorrhization at limited and optimal N supply. Pinus pinaster seedlings were inoculated with living or dead (control) cultures of Pisolithus tinctorius, supplied with ammonium at 4 (limiting) or 7% d−1 (non-limiting) N relative addition rate (RARN), and followed development for 29 days. Mycorrhizal colonization of roots was quantified by the determination of ergosterol. A series of enzymes (sucrose and trehalose metabolism, anaplerosis) and metabolites (soluble carbohydrate, including trehalose; fructose 2,6 bisphosphate, free amino acids) relevant in the C/N exchange between symbionts, and in the carbon allocation and sink strength within the plant were assayed for 2-day-intervals for up to 14 days, and at 5-day-intervals for the rest of the experiment. The first 10 days reflected the establishment of mycorrhizal interaction, and the carbon allocation to the root was higher in M plants independent of N supply. Following this period, carbon allocation became N-related, higher at low, and lower at high N supply. The belowground C investment of M plants was dependent on N availability, but not on N gain. Finally, increased belowground C allocation was accompanied by a shift from plant to fungal metabolism.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Corrêa</LastName><ForeName>Ana</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Universidade de Lisboa, Faculdade de Ciências, Centro de Biologia Aplicada, Campo Grande C2. Piso 4, 1749-016 Lisbon, Portugal. ambcorrea@gmail.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hampp</LastName><ForeName>Rüdiger</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Magel</LastName><ForeName>Elisabeth</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Martins-Loução</LastName><ForeName>Maria-Amélia</ForeName><Initials>MA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Mycorrhiza</MedlineTA><NlmUniqueID>100955036</NlmUniqueID><ISSNLinking>0940-6360</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009584" 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