Infection-specific activation of the Medicago truncatula Enod11 early nodulin gene promoter during actinorhizal root nodulation.
Identifieur interne : 002665 ( Main/Corpus ); précédent : 002664; suivant : 002666Infection-specific activation of the Medicago truncatula Enod11 early nodulin gene promoter during actinorhizal root nodulation.
Auteurs : Sergio Svistoonoff ; Mame-Ourèye Sy ; Nathalie Diagne ; David G. Barker ; Didier Bogusz ; Claudine FrancheSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2010.
English descriptors
- KwdEn :
- Frankia (physiology), Gene Expression Regulation, Bacterial (MeSH), Gene Expression Regulation, Plant (physiology), Medicago truncatula (genetics), Medicago truncatula (metabolism), Mycorrhizae (physiology), Plant Diseases (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Root Nodulation (genetics), Plant Root Nodulation (physiology), Plant Roots (genetics), Plant Roots (metabolism), Promoter Regions, Genetic (MeSH).
- MESH :
- chemical , genetics : Plant Proteins.
- genetics : Medicago truncatula, Plant Root Nodulation, Plant Roots.
- metabolism : Medicago truncatula, Plant Proteins, Plant Roots.
- physiology : Frankia, Gene Expression Regulation, Plant, Mycorrhizae, Plant Root Nodulation.
- Gene Expression Regulation, Bacterial, Plant Diseases, Promoter Regions, Genetic.
Abstract
The MtEnod11 gene from Medicago truncatula is widely used as an early infection-related molecular marker for endosymbiotic associations involving both rhizobia and arbuscular mycorrhizal fungi. In this article, heterologous expression of the MtEnod11 promoter has been studied in two actinorhizal trees, Casuarina glauca and Allocasuarina verticillata. Transgenic C. glauca and A. verticillata expressing a ProMtEnod11::beta-glucuronidase (gus) fusion were generated and the activation of the transgene investigated in the context of the symbiotic associations with the N-fixing actinomycete Frankia and both endo- and ectomycorrhizal fungi (Glomus intraradices and Pisolithus albus, respectively). ProMtEnod11::gus expression was observed in root hairs, prenodules, and nodules and could be correlated with the infection of plant cells by Frankia spp. However, no activation of the gus reporter gene was detected prior to infection or in response to either rhizobial Nod factors or the wasp venom peptide MAS-7. Equally, ProMtEnod11::gus expression was not elicited during the symbiotic associations with either ecto- or endomycorrhizal fungi. These observations suggest that, although there is a conservation of gene regulatory pathways between legumes and actinorhizal plants in cells accommodating endosymbiotic N-fixing bacteria, the events preceding bacterial infection or related to mycorrhization appear to be less conserved.
DOI: 10.1094/MPMI-23-6-0740
PubMed: 20459313
Links to Exploration step
pubmed:20459313Le document en format XML
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Barker</name></author><author><name sortKey="Bogusz, Didier" sort="Bogusz, Didier" uniqKey="Bogusz D" first="Didier" last="Bogusz">Didier Bogusz</name></author><author><name sortKey="Franche, Claudine" sort="Franche, Claudine" uniqKey="Franche C" first="Claudine" last="Franche">Claudine Franche</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20459313</idno><idno type="pmid">20459313</idno><idno type="doi">10.1094/MPMI-23-6-0740</idno><idno type="wicri:Area/Main/Corpus">002665</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002665</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Infection-specific activation of the Medicago truncatula Enod11 early nodulin gene promoter during actinorhizal root nodulation.</title><author><name sortKey="Svistoonoff, Sergio" sort="Svistoonoff, Sergio" uniqKey="Svistoonoff S" first="Sergio" last="Svistoonoff">Sergio Svistoonoff</name><affiliation><nlm:affiliation>Groupe Rhizogenèse, Unité Mixte de Recherche Diversité et Adaptation des Plantes Cultivées, Institut de Recherche pour le Développement, 911 avenue Agropolis, BP 5045, 34394 Montpellier Cedex 5, France. sergio.svistoonoff@ird.fr</nlm:affiliation></affiliation></author><author><name sortKey="Sy, Mame Oureye" sort="Sy, Mame Oureye" uniqKey="Sy M" first="Mame-Ourèye" last="Sy">Mame-Ourèye Sy</name></author><author><name sortKey="Diagne, Nathalie" sort="Diagne, Nathalie" uniqKey="Diagne N" first="Nathalie" last="Diagne">Nathalie Diagne</name></author><author><name sortKey="Barker, David G" sort="Barker, David G" uniqKey="Barker D" first="David G" last="Barker">David G. Barker</name></author><author><name sortKey="Bogusz, Didier" sort="Bogusz, Didier" uniqKey="Bogusz D" first="Didier" last="Bogusz">Didier Bogusz</name></author><author><name sortKey="Franche, Claudine" sort="Franche, Claudine" uniqKey="Franche C" first="Claudine" last="Franche">Claudine Franche</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Frankia (physiology)</term><term>Gene Expression Regulation, Bacterial (MeSH)</term><term>Gene Expression Regulation, Plant (physiology)</term><term>Medicago truncatula (genetics)</term><term>Medicago truncatula (metabolism)</term><term>Mycorrhizae (physiology)</term><term>Plant Diseases (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant Root Nodulation (genetics)</term><term>Plant Root Nodulation (physiology)</term><term>Plant Roots (genetics)</term><term>Plant Roots (metabolism)</term><term>Promoter Regions, Genetic (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Medicago truncatula</term><term>Plant Root Nodulation</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Medicago truncatula</term><term>Plant Proteins</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Frankia</term><term>Gene Expression Regulation, Plant</term><term>Mycorrhizae</term><term>Plant Root Nodulation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Bacterial</term><term>Plant Diseases</term><term>Promoter Regions, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The MtEnod11 gene from Medicago truncatula is widely used as an early infection-related molecular marker for endosymbiotic associations involving both rhizobia and arbuscular mycorrhizal fungi. In this article, heterologous expression of the MtEnod11 promoter has been studied in two actinorhizal trees, Casuarina glauca and Allocasuarina verticillata. Transgenic C. glauca and A. verticillata expressing a ProMtEnod11::beta-glucuronidase (gus) fusion were generated and the activation of the transgene investigated in the context of the symbiotic associations with the N-fixing actinomycete Frankia and both endo- and ectomycorrhizal fungi (Glomus intraradices and Pisolithus albus, respectively). ProMtEnod11::gus expression was observed in root hairs, prenodules, and nodules and could be correlated with the infection of plant cells by Frankia spp. However, no activation of the gus reporter gene was detected prior to infection or in response to either rhizobial Nod factors or the wasp venom peptide MAS-7. Equally, ProMtEnod11::gus expression was not elicited during the symbiotic associations with either ecto- or endomycorrhizal fungi. These observations suggest that, although there is a conservation of gene regulatory pathways between legumes and actinorhizal plants in cells accommodating endosymbiotic N-fixing bacteria, the events preceding bacterial infection or related to mycorrhization appear to be less conserved.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20459313</PMID><DateCompleted><Year>2010</Year><Month>06</Month><Day>29</Day></DateCompleted><DateRevised><Year>2010</Year><Month>05</Month><Day>12</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>23</Volume><Issue>6</Issue><PubDate><Year>2010</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Infection-specific activation of the Medicago truncatula Enod11 early nodulin gene promoter during actinorhizal root nodulation.</ArticleTitle><Pagination><MedlinePgn>740-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-23-6-0740</ELocationID><Abstract><AbstractText>The MtEnod11 gene from Medicago truncatula is widely used as an early infection-related molecular marker for endosymbiotic associations involving both rhizobia and arbuscular mycorrhizal fungi. In this article, heterologous expression of the MtEnod11 promoter has been studied in two actinorhizal trees, Casuarina glauca and Allocasuarina verticillata. Transgenic C. glauca and A. verticillata expressing a ProMtEnod11::beta-glucuronidase (gus) fusion were generated and the activation of the transgene investigated in the context of the symbiotic associations with the N-fixing actinomycete Frankia and both endo- and ectomycorrhizal fungi (Glomus intraradices and Pisolithus albus, respectively). ProMtEnod11::gus expression was observed in root hairs, prenodules, and nodules and could be correlated with the infection of plant cells by Frankia spp. However, no activation of the gus reporter gene was detected prior to infection or in response to either rhizobial Nod factors or the wasp venom peptide MAS-7. Equally, ProMtEnod11::gus expression was not elicited during the symbiotic associations with either ecto- or endomycorrhizal fungi. These observations suggest that, although there is a conservation of gene regulatory pathways between legumes and actinorhizal plants in cells accommodating endosymbiotic N-fixing bacteria, the events preceding bacterial infection or related to mycorrhization appear to be less conserved.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Svistoonoff</LastName><ForeName>Sergio</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Groupe Rhizogenèse, Unité Mixte de Recherche Diversité et Adaptation des Plantes Cultivées, Institut de Recherche pour le Développement, 911 avenue Agropolis, BP 5045, 34394 Montpellier Cedex 5, France. sergio.svistoonoff@ird.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sy</LastName><ForeName>Mame-Ourèye</ForeName><Initials>MO</Initials></Author><Author ValidYN="Y"><LastName>Diagne</LastName><ForeName>Nathalie</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Barker</LastName><ForeName>David G</ForeName><Initials>DG</Initials></Author><Author ValidYN="Y"><LastName>Bogusz</LastName><ForeName>Didier</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Franche</LastName><ForeName>Claudine</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C438018">ENOD11 protein, Medicago truncatula</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D040161" MajorTopicYN="N">Frankia</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015964" MajorTopicYN="N">Gene Expression Regulation, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046913" MajorTopicYN="N">Medicago truncatula</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055170" MajorTopicYN="N">Plant Root Nodulation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="Y">Promoter Regions, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>5</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>5</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>6</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20459313</ArticleId><ArticleId IdType="doi">10.1094/MPMI-23-6-0740</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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