Identification of two conserved cis-acting elements, MYCS and P1BS, involved in the regulation of mycorrhiza-activated phosphate transporters in eudicot species.
Identifieur interne : 002491 ( Main/Corpus ); précédent : 002490; suivant : 002492Identification of two conserved cis-acting elements, MYCS and P1BS, involved in the regulation of mycorrhiza-activated phosphate transporters in eudicot species.
Auteurs : Aiqun Chen ; Mian Gu ; Shubin Sun ; Lingling Zhu ; Shuai Hong ; Guohua XuSource :
- The New phytologist [ 1469-8137 ] ; 2011.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Conserved Sequence (genetics), Gene Expression Regulation, Plant (MeSH), Glucuronidase (MeSH), Molecular Sequence Data (MeSH), Mycorrhizae (metabolism), Phosphate Transport Proteins (genetics), Phosphate Transport Proteins (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (MeSH), Promoter Regions, Genetic (MeSH), Solanum melongena (genetics), Species Specificity (MeSH), Tobacco (genetics).
- MESH :
- chemical , genetics : Phosphate Transport Proteins, Plant Proteins.
- chemical , metabolism : Phosphate Transport Proteins, Plant Proteins.
- chemical : Glucuronidase.
- genetics : Conserved Sequence, Solanum melongena, Tobacco.
- metabolism : Mycorrhizae.
- Base Sequence, Gene Expression Regulation, Plant, Molecular Sequence Data, Plants, Genetically Modified, Promoter Regions, Genetic, Species Specificity.
Abstract
• In this study, six putative promoter regions of phosphate transporter Pht1;3, Pht1;4 and Pht1;5 genes were isolated from eggplant and tobacco using the inverse polymerase chain reaction (iPCR). The isolated sequences show evolutionary conservation and divergence within/between the two groups of Pht1;3 and Pht1;4/Pht1;5. • Histochemical analyses showed that all six promoter fragments were sufficient to drive β-glucuronidase (GUS) expression specifically in arbuscular mycorrhizal (AM) tobacco roots and were confined to distinct cells containing AM fungal structures (arbuscules or intracellular hyphae). • A series of promoter truncation and mutation analyses combined with phylogenetic footprinting of these promoters revealed that at least two cis-regulatory elements--the mycorrhiza transcription factor binding sequence (MYCS) first identified in this study and P1BS--mediated the transcriptional activation of the AM-mediated inorganic phosphate (Pi) transporter genes. Deletion or partial mutation of either of the two motifs in the promoters could cause a remarkable decrease, or even complete absence, of the promoter activity. • Our results propose that uptake of inorganic phosphate (Pi) by AM fungi is regulated, at least partially, in an MYCS- and P1BS-dependent manner in eudicot species. Our finding offers new insights into the molecular mechanisms underlying the coordination between the AM and the Pi signalling pathways.
DOI: 10.1111/j.1469-8137.2010.03556.x
PubMed: 21106037
Links to Exploration step
pubmed:21106037Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of two conserved cis-acting elements, MYCS and P1BS, involved in the regulation of mycorrhiza-activated phosphate transporters in eudicot species.</title><author><name sortKey="Chen, Aiqun" sort="Chen, Aiqun" uniqKey="Chen A" first="Aiqun" last="Chen">Aiqun Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Gu, Mian" sort="Gu, Mian" uniqKey="Gu M" first="Mian" last="Gu">Mian Gu</name></author><author><name sortKey="Sun, Shubin" sort="Sun, Shubin" uniqKey="Sun S" first="Shubin" last="Sun">Shubin Sun</name></author><author><name sortKey="Zhu, Lingling" sort="Zhu, Lingling" uniqKey="Zhu L" first="Lingling" last="Zhu">Lingling Zhu</name></author><author><name sortKey="Hong, Shuai" sort="Hong, Shuai" uniqKey="Hong S" first="Shuai" last="Hong">Shuai Hong</name></author><author><name sortKey="Xu, Guohua" sort="Xu, Guohua" uniqKey="Xu G" first="Guohua" last="Xu">Guohua Xu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21106037</idno><idno type="pmid">21106037</idno><idno type="doi">10.1111/j.1469-8137.2010.03556.x</idno><idno type="wicri:Area/Main/Corpus">002491</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002491</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of two conserved cis-acting elements, MYCS and P1BS, involved in the regulation of mycorrhiza-activated phosphate transporters in eudicot species.</title><author><name sortKey="Chen, Aiqun" sort="Chen, Aiqun" uniqKey="Chen A" first="Aiqun" last="Chen">Aiqun Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Gu, Mian" sort="Gu, Mian" uniqKey="Gu M" first="Mian" last="Gu">Mian Gu</name></author><author><name sortKey="Sun, Shubin" sort="Sun, Shubin" uniqKey="Sun S" first="Shubin" last="Sun">Shubin Sun</name></author><author><name sortKey="Zhu, Lingling" sort="Zhu, Lingling" uniqKey="Zhu L" first="Lingling" last="Zhu">Lingling Zhu</name></author><author><name sortKey="Hong, Shuai" sort="Hong, Shuai" uniqKey="Hong S" first="Shuai" last="Hong">Shuai Hong</name></author><author><name sortKey="Xu, Guohua" sort="Xu, Guohua" uniqKey="Xu G" first="Guohua" last="Xu">Guohua Xu</name></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Conserved Sequence (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Glucuronidase (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (metabolism)</term><term>Phosphate Transport Proteins (genetics)</term><term>Phosphate Transport Proteins (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Solanum melongena (genetics)</term><term>Species Specificity (MeSH)</term><term>Tobacco (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Phosphate Transport Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Phosphate Transport Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Glucuronidase</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Conserved Sequence</term><term>Solanum melongena</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term><term>Plants, Genetically Modified</term><term>Promoter Regions, Genetic</term><term>Species Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">• In this study, six putative promoter regions of phosphate transporter Pht1;3, Pht1;4 and Pht1;5 genes were isolated from eggplant and tobacco using the inverse polymerase chain reaction (iPCR). The isolated sequences show evolutionary conservation and divergence within/between the two groups of Pht1;3 and Pht1;4/Pht1;5. • Histochemical analyses showed that all six promoter fragments were sufficient to drive β-glucuronidase (GUS) expression specifically in arbuscular mycorrhizal (AM) tobacco roots and were confined to distinct cells containing AM fungal structures (arbuscules or intracellular hyphae). • A series of promoter truncation and mutation analyses combined with phylogenetic footprinting of these promoters revealed that at least two cis-regulatory elements--the mycorrhiza transcription factor binding sequence (MYCS) first identified in this study and P1BS--mediated the transcriptional activation of the AM-mediated inorganic phosphate (Pi) transporter genes. Deletion or partial mutation of either of the two motifs in the promoters could cause a remarkable decrease, or even complete absence, of the promoter activity. • Our results propose that uptake of inorganic phosphate (Pi) by AM fungi is regulated, at least partially, in an MYCS- and P1BS-dependent manner in eudicot species. Our finding offers new insights into the molecular mechanisms underlying the coordination between the AM and the Pi signalling pathways.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21106037</PMID><DateCompleted><Year>2011</Year><Month>05</Month><Day>18</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>189</Volume><Issue>4</Issue><PubDate><Year>2011</Year><Month>Mar</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Identification of two conserved cis-acting elements, MYCS and P1BS, involved in the regulation of mycorrhiza-activated phosphate transporters in eudicot species.</ArticleTitle><Pagination><MedlinePgn>1157-69</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1469-8137.2010.03556.x</ELocationID><Abstract><AbstractText>• In this study, six putative promoter regions of phosphate transporter Pht1;3, Pht1;4 and Pht1;5 genes were isolated from eggplant and tobacco using the inverse polymerase chain reaction (iPCR). The isolated sequences show evolutionary conservation and divergence within/between the two groups of Pht1;3 and Pht1;4/Pht1;5. • Histochemical analyses showed that all six promoter fragments were sufficient to drive β-glucuronidase (GUS) expression specifically in arbuscular mycorrhizal (AM) tobacco roots and were confined to distinct cells containing AM fungal structures (arbuscules or intracellular hyphae). • A series of promoter truncation and mutation analyses combined with phylogenetic footprinting of these promoters revealed that at least two cis-regulatory elements--the mycorrhiza transcription factor binding sequence (MYCS) first identified in this study and P1BS--mediated the transcriptional activation of the AM-mediated inorganic phosphate (Pi) transporter genes. Deletion or partial mutation of either of the two motifs in the promoters could cause a remarkable decrease, or even complete absence, of the promoter activity. • Our results propose that uptake of inorganic phosphate (Pi) by AM fungi is regulated, at least partially, in an MYCS- and P1BS-dependent manner in eudicot species. Our finding offers new insights into the molecular mechanisms underlying the coordination between the AM and the Pi signalling pathways.</AbstractText><CopyrightInformation>© 2010 The Authors. New Phytologist © 2010 New Phytologist Trust.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Aiqun</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gu</LastName><ForeName>Mian</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Shubin</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Lingling</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Hong</LastName><ForeName>Shuai</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Guohua</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>11</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D028061">Phosphate Transport Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017124" MajorTopicYN="N">Conserved Sequence</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028061" MajorTopicYN="N">Phosphate Transport Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="Y">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032323" MajorTopicYN="N">Solanum melongena</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>11</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>11</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>5</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21106037</ArticleId><ArticleId IdType="doi">10.1111/j.1469-8137.2010.03556.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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