Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers.
Identifieur interne : 002386 ( Main/Corpus ); précédent : 002385; suivant : 002387Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers.
Auteurs : Milan Gryndler ; Hana Hršelová ; Lucie Soukupová ; Eva Streiblová ; Slavomír Valda ; Jan Borovi Ka ; Hana Gryndlerová ; Ján Gažo ; Marián MikoSource :
- FEMS microbiology letters [ 1574-6968 ] ; 2011.
English descriptors
- KwdEn :
- Ascomycota (classification), Ascomycota (genetics), Ascomycota (isolation & purification), DNA Primers (genetics), Molecular Sequence Data (MeSH), Mycorrhizae (classification), Mycorrhizae (genetics), Mycorrhizae (isolation & purification), Polymerase Chain Reaction (instrumentation), Polymerase Chain Reaction (methods), Soil Microbiology (MeSH).
- MESH :
- chemical , genetics : DNA Primers.
- classification : Ascomycota, Mycorrhizae.
- genetics : Ascomycota, Mycorrhizae.
- instrumentation : Polymerase Chain Reaction.
- isolation & purification : Ascomycota, Mycorrhizae.
- methods : Polymerase Chain Reaction.
- Molecular Sequence Data, Soil Microbiology.
Abstract
Tuber aestivum is becoming an important commodity of great economical value in some European countries. At the same time, it is a highly protected organism in other countries, where it needs careful treatment. A reliable method of detection in roots and soil is thus needed for assessment of geographic distribution, ecological studies and inoculation efficiency testing in man-made experiments. A PCR-based method of detection of T. aestivum using specific primers was therefore developed. A pair of PCR primers Tu1sekvF/Tu2sekvR selective for T. aestivum and some genotypes of Tuber mesentericum was designed on the basis of the known internal transcribed spacer T. aestivum sequences. TaiI restriction cleavage was then used to distinguish the two species. The selectivity of the designed primer pair was evaluated using DNA extracted from specimens of a further 13 Tuber spp. Subsequently, the selectivity and robustness to false-positive results with nontarget DNA of the designed primers was compared with two other primer pairs (UncI/UncII and BTAE-F/BTAEMB-R). The occurrence of T. aestivum in soil and ectomycorrhizae collected in its native habitat has been successfully detected using the designed primers and nested PCR. The method is reliable and thus suitable for detection of T. aestivum in the field.
DOI: 10.1111/j.1574-6968.2011.02243.x
PubMed: 21385201
Links to Exploration step
pubmed:21385201Le document en format XML
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last="Valda">Slavomír Valda</name></author><author><name sortKey="Borovi Ka, Jan" sort="Borovi Ka, Jan" uniqKey="Borovi Ka J" first="Jan" last="Borovi Ka">Jan Borovi Ka</name></author><author><name sortKey="Gryndlerova, Hana" sort="Gryndlerova, Hana" uniqKey="Gryndlerova H" first="Hana" last="Gryndlerová">Hana Gryndlerová</name></author><author><name sortKey="Gazo, Jan" sort="Gazo, Jan" uniqKey="Gazo J" first="Ján" last="Gažo">Ján Gažo</name></author><author><name sortKey="Miko, Marian" sort="Miko, Marian" uniqKey="Miko M" first="Marián" last="Miko">Marián Miko</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21385201</idno><idno type="pmid">21385201</idno><idno type="doi">10.1111/j.1574-6968.2011.02243.x</idno><idno type="wicri:Area/Main/Corpus">002386</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" 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Slavomir" uniqKey="Valda S" first="Slavomír" last="Valda">Slavomír Valda</name></author><author><name sortKey="Borovi Ka, Jan" sort="Borovi Ka, Jan" uniqKey="Borovi Ka J" first="Jan" last="Borovi Ka">Jan Borovi Ka</name></author><author><name sortKey="Gryndlerova, Hana" sort="Gryndlerova, Hana" uniqKey="Gryndlerova H" first="Hana" last="Gryndlerová">Hana Gryndlerová</name></author><author><name sortKey="Gazo, Jan" sort="Gazo, Jan" uniqKey="Gazo J" first="Ján" last="Gažo">Ján Gažo</name></author><author><name sortKey="Miko, Marian" sort="Miko, Marian" uniqKey="Miko M" first="Marián" last="Miko">Marián Miko</name></author></analytic><series><title level="j">FEMS microbiology letters</title><idno type="eISSN">1574-6968</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (classification)</term><term>Ascomycota (genetics)</term><term>Ascomycota (isolation & purification)</term><term>DNA Primers (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (classification)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (isolation & purification)</term><term>Polymerase Chain Reaction (instrumentation)</term><term>Polymerase Chain Reaction (methods)</term><term>Soil Microbiology (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Ascomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ascomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Ascomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Sequence Data</term><term>Soil Microbiology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Tuber aestivum is becoming an important commodity of great economical value in some European countries. At the same time, it is a highly protected organism in other countries, where it needs careful treatment. A reliable method of detection in roots and soil is thus needed for assessment of geographic distribution, ecological studies and inoculation efficiency testing in man-made experiments. A PCR-based method of detection of T. aestivum using specific primers was therefore developed. A pair of PCR primers Tu1sekvF/Tu2sekvR selective for T. aestivum and some genotypes of Tuber mesentericum was designed on the basis of the known internal transcribed spacer T. aestivum sequences. TaiI restriction cleavage was then used to distinguish the two species. The selectivity of the designed primer pair was evaluated using DNA extracted from specimens of a further 13 Tuber spp. Subsequently, the selectivity and robustness to false-positive results with nontarget DNA of the designed primers was compared with two other primer pairs (UncI/UncII and BTAE-F/BTAEMB-R). The occurrence of T. aestivum in soil and ectomycorrhizae collected in its native habitat has been successfully detected using the designed primers and nested PCR. The method is reliable and thus suitable for detection of T. aestivum in the field.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21385201</PMID><DateCompleted><Year>2011</Year><Month>07</Month><Day>12</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1574-6968</ISSN><JournalIssue CitedMedium="Internet"><Volume>318</Volume><Issue>1</Issue><PubDate><Year>2011</Year><Month>May</Month></PubDate></JournalIssue><Title>FEMS microbiology letters</Title><ISOAbbreviation>FEMS Microbiol Lett</ISOAbbreviation></Journal><ArticleTitle>Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers.</ArticleTitle><Pagination><MedlinePgn>84-91</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1574-6968.2011.02243.x</ELocationID><Abstract><AbstractText>Tuber aestivum is becoming an important commodity of great economical value in some European countries. At the same time, it is a highly protected organism in other countries, where it needs careful treatment. A reliable method of detection in roots and soil is thus needed for assessment of geographic distribution, ecological studies and inoculation efficiency testing in man-made experiments. A PCR-based method of detection of T. aestivum using specific primers was therefore developed. A pair of PCR primers Tu1sekvF/Tu2sekvR selective for T. aestivum and some genotypes of Tuber mesentericum was designed on the basis of the known internal transcribed spacer T. aestivum sequences. TaiI restriction cleavage was then used to distinguish the two species. The selectivity of the designed primer pair was evaluated using DNA extracted from specimens of a further 13 Tuber spp. Subsequently, the selectivity and robustness to false-positive results with nontarget DNA of the designed primers was compared with two other primer pairs (UncI/UncII and BTAE-F/BTAEMB-R). The occurrence of T. aestivum in soil and ectomycorrhizae collected in its native habitat has been successfully detected using the designed primers and nested PCR. The method is reliable and thus suitable for detection of T. aestivum in the field.</AbstractText><CopyrightInformation>© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gryndler</LastName><ForeName>Milan</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Microbiology, ASCR, Prague, Czech Republic. gryndler@biomed.cas.cz</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hršelová</LastName><ForeName>Hana</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Soukupová</LastName><ForeName>Lucie</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Streiblová</LastName><ForeName>Eva</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Valda</LastName><ForeName>Slavomír</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Borovička</LastName><ForeName>Jan</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Gryndlerová</LastName><ForeName>Hana</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Gažo</LastName><ForeName>Ján</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Miko</LastName><ForeName>Marián</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>03</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEMS Microbiol Lett</MedlineTA><NlmUniqueID>7705721</NlmUniqueID><ISSNLinking>0378-1097</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil Microbiology</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>7</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21385201</ArticleId><ArticleId IdType="doi">10.1111/j.1574-6968.2011.02243.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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