Macromolecular trafficking between a vesicular arbuscular endomycorrhizal fungus and roots of transgenic tobacco.
Identifieur interne : 002367 ( Main/Corpus ); précédent : 002366; suivant : 002368Macromolecular trafficking between a vesicular arbuscular endomycorrhizal fungus and roots of transgenic tobacco.
Auteurs : Rocío Morales-Rayas ; Roberto Ruiz-Medrano ; Beatriz Xoconostle-CázaresSource :
- Plant signaling & behavior [ 1559-2324 ] ; 2011.
English descriptors
- KwdEn :
- Biological Transport (MeSH), Colony Count, Microbial (MeSH), Fluoresceins (metabolism), Fluorescence (MeSH), Glomeromycota (physiology), Green Fluorescent Proteins (metabolism), Macromolecular Substances (metabolism), Mycorrhizae (metabolism), Plants, Genetically Modified (MeSH), Protein Transport (MeSH), Symbiosis (MeSH), Tobacco (genetics), Tobacco (metabolism), Tobacco (microbiology).
- MESH :
- chemical , metabolism : Fluoresceins, Green Fluorescent Proteins, Macromolecular Substances.
- genetics : Tobacco.
- metabolism : Mycorrhizae, Tobacco.
- microbiology : Tobacco.
- physiology : Glomeromycota.
- Biological Transport, Colony Count, Microbial, Fluorescence, Plants, Genetically Modified, Protein Transport, Symbiosis.
Abstract
The root system of transgenic tobacco plants expressing the enhanced green fluorescent protein (EGFP) under the control of the 35S cauliflower mosaic virus (CaMV) promoter, were colonized with the endomycorrhizal fungus Glomus intraradices. Translocation of EGFP protein from the root to the fungus was registered by light and confocal microscopy. Immunolocalization also showed the presence of EGFP in the mycelium of Glomus intraradices. Carboxyfluorescein feeding experiments on wild type mycorrhized plants evidenced the transport of fluorescein, a symplasmic tracer, from the plant to the fungus. Our results suggest that endomycorrhiza possess the capacity to exchange functional biological macromolecules as evidenced by the transport EGFP from the plant to the fungal symbiont.
DOI: 10.4161/psb.6.5.15022
PubMed: 21448001
PubMed Central: PMC3172824
Links to Exploration step
pubmed:21448001Le document en format XML
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Translocation of EGFP protein from the root to the fungus was registered by light and confocal microscopy. Immunolocalization also showed the presence of EGFP in the mycelium of Glomus intraradices. Carboxyfluorescein feeding experiments on wild type mycorrhized plants evidenced the transport of fluorescein, a symplasmic tracer, from the plant to the fungus. Our results suggest that endomycorrhiza possess the capacity to exchange functional biological macromolecules as evidenced by the transport EGFP from the plant to the fungal symbiont.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21448001</PMID><DateCompleted><Year>2011</Year><Month>11</Month><Day>28</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1559-2324</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><Issue>5</Issue><PubDate><Year>2011</Year><Month>May</Month></PubDate></JournalIssue><Title>Plant signaling & behavior</Title><ISOAbbreviation>Plant Signal Behav</ISOAbbreviation></Journal><ArticleTitle>Macromolecular trafficking between a vesicular arbuscular endomycorrhizal fungus and roots of transgenic tobacco.</ArticleTitle><Pagination><MedlinePgn>617-23</MedlinePgn></Pagination><Abstract><AbstractText>The root system of transgenic tobacco plants expressing the enhanced green fluorescent protein (EGFP) under the control of the 35S cauliflower mosaic virus (CaMV) promoter, were colonized with the endomycorrhizal fungus Glomus intraradices. Translocation of EGFP protein from the root to the fungus was registered by light and confocal microscopy. Immunolocalization also showed the presence of EGFP in the mycelium of Glomus intraradices. Carboxyfluorescein feeding experiments on wild type mycorrhized plants evidenced the transport of fluorescein, a symplasmic tracer, from the plant to the fungus. Our results suggest that endomycorrhiza possess the capacity to exchange functional biological macromolecules as evidenced by the transport EGFP from the plant to the fungal symbiont.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Morales-Rayas</LastName><ForeName>Rocío</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Departamento de Biotecnología y Bioingeniería, Centro de Investigación y Estudios Avanzados, San Pedro Zacatenco, México.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ruiz-Medrano</LastName><ForeName>Roberto</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Xoconostle-Cázares</LastName><ForeName>Beatriz</ForeName><Initials>B</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate 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