Host associations between fungal root endophytes and boreal trees.
Identifieur interne : 002350 ( Main/Corpus ); précédent : 002349; suivant : 002351Host associations between fungal root endophytes and boreal trees.
Auteurs : Gavin Kernaghan ; Glenn PatriquinSource :
- Microbial ecology [ 1432-184X ] ; 2011.
English descriptors
- KwdEn :
- Abies (microbiology), Ascomycota (classification), Ascomycota (genetics), Ascomycota (growth & development), Ascomycota (isolation & purification), Betula (microbiology), DNA, Ribosomal Spacer (genetics), Endophytes (classification), Endophytes (genetics), Endophytes (growth & development), Endophytes (isolation & purification), Multivariate Analysis (MeSH), Mycorrhizae (growth & development), Nova Scotia (MeSH), Phylogeny (MeSH), Picea (microbiology), Plant Roots (microbiology), Polymorphism, Restriction Fragment Length (MeSH), Quebec (MeSH), Sequence Analysis, DNA (MeSH), Trees (microbiology).
- MESH :
- chemical , genetics : DNA, Ribosomal Spacer.
- geographic : Nova Scotia, Quebec.
- classification : Ascomycota, Endophytes.
- genetics : Ascomycota, Endophytes.
- growth & development : Ascomycota, Endophytes, Mycorrhizae.
- isolation & purification : Ascomycota, Endophytes.
- microbiology : Abies, Betula, Picea, Plant Roots, Trees.
- Multivariate Analysis, Phylogeny, Polymorphism, Restriction Fragment Length, Sequence Analysis, DNA.
Abstract
Fungal root endophytes colonize root tissue concomitantly with mycorrhizal fungi, but their identities and host preferences are largely unknown. We cultured fungal endophytes from surface-sterilized Cenococcum geophilum ectomycorrhizae of Betula papyrifera, Abies balsamea, and Picea glauca from two boreal sites in eastern Canada. Isolates were initially grouped on the basis of cultural morphology and then identified by internal transcribed spacer ribosomal DNA sequencing or by PCR restriction fragment length polymorphism. Phylogenetic analysis of the sequence data revealed 31 distinct phylotypes among the isolates, comprising mainly members of the ascomycete families Helotiaceae, Dermateaceae, Myxotrichaceae, and Hyaloscyphaceae, although other fungi were also isolated. Multivariate analyses indicate a clear separation among the endophyte communities colonizing each host tree species. Some phylotypes were evenly distributed across the roots of all three host species, some were found preferentially on particular hosts, and others were isolated from single hosts only. The results indicate that fungal root endophytes of boreal trees are not randomly distributed, but instead form relatively distinct assemblages on different host tree species.
DOI: 10.1007/s00248-011-9851-6
PubMed: 21475991
Links to Exploration step
pubmed:21475991Le document en format XML
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We cultured fungal endophytes from surface-sterilized Cenococcum geophilum ectomycorrhizae of Betula papyrifera, Abies balsamea, and Picea glauca from two boreal sites in eastern Canada. Isolates were initially grouped on the basis of cultural morphology and then identified by internal transcribed spacer ribosomal DNA sequencing or by PCR restriction fragment length polymorphism. Phylogenetic analysis of the sequence data revealed 31 distinct phylotypes among the isolates, comprising mainly members of the ascomycete families Helotiaceae, Dermateaceae, Myxotrichaceae, and Hyaloscyphaceae, although other fungi were also isolated. Multivariate analyses indicate a clear separation among the endophyte communities colonizing each host tree species. Some phylotypes were evenly distributed across the roots of all three host species, some were found preferentially on particular hosts, and others were isolated from single hosts only. The results indicate that fungal root endophytes of boreal trees are not randomly distributed, but instead form relatively distinct assemblages on different host tree species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21475991</PMID><DateCompleted><Year>2011</Year><Month>12</Month><Day>15</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-184X</ISSN><JournalIssue CitedMedium="Internet"><Volume>62</Volume><Issue>2</Issue><PubDate><Year>2011</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Microbial ecology</Title><ISOAbbreviation>Microb Ecol</ISOAbbreviation></Journal><ArticleTitle>Host associations between fungal root endophytes and boreal trees.</ArticleTitle><Pagination><MedlinePgn>460-73</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00248-011-9851-6</ELocationID><Abstract><AbstractText>Fungal root endophytes colonize root tissue concomitantly with mycorrhizal fungi, but their identities and host preferences are largely unknown. We cultured fungal endophytes from surface-sterilized Cenococcum geophilum ectomycorrhizae of Betula papyrifera, Abies balsamea, and Picea glauca from two boreal sites in eastern Canada. Isolates were initially grouped on the basis of cultural morphology and then identified by internal transcribed spacer ribosomal DNA sequencing or by PCR restriction fragment length polymorphism. Phylogenetic analysis of the sequence data revealed 31 distinct phylotypes among the isolates, comprising mainly members of the ascomycete families Helotiaceae, Dermateaceae, Myxotrichaceae, and Hyaloscyphaceae, although other fungi were also isolated. Multivariate analyses indicate a clear separation among the endophyte communities colonizing each host tree species. Some phylotypes were evenly distributed across the roots of all three host species, some were found preferentially on particular hosts, and others were isolated from single hosts only. The results indicate that fungal root endophytes of boreal trees are not randomly distributed, but instead form relatively distinct assemblages on different host tree species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kernaghan</LastName><ForeName>Gavin</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Biology Department, Mount St. Vincent University, 166 Bedford Hwy., Halifax, NS, B3M 2J6, Canada. gavin.kernaghan@msvu.ca</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Patriquin</LastName><ForeName>Glenn</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>04</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>United 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