Novel root-fungus symbiosis in Ericaceae: sheathed ericoid mycorrhiza formed by a hitherto undescribed basidiomycete with affinities to Trechisporales.
Identifieur interne : 001F24 ( Main/Corpus ); précédent : 001F23; suivant : 001F25Novel root-fungus symbiosis in Ericaceae: sheathed ericoid mycorrhiza formed by a hitherto undescribed basidiomycete with affinities to Trechisporales.
Auteurs : Martin Vohník ; Jesse J. Sadowsky ; Petr Kohout ; Zuzana Lhotáková ; Rolf Nestby ; Miroslav Kola KSource :
- PloS one [ 1932-6203 ] ; 2012.
English descriptors
- KwdEn :
- MESH :
- geographic : Norway.
- classification : Mycorrhizae.
- genetics : Basidiomycota, Mycorrhizae, Symbiosis.
- microbiology : Ericaceae, Plant Roots.
- Phylogeny.
Abstract
Ericaceae (the heath family) are widely distributed calcifuges inhabiting soils with inherently poor nutrient status. Ericaceae overcome nutrient limitation through symbiosis with ericoid mycorrhizal (ErM) fungi that mobilize nutrients complexed in recalcitrant organic matter. At present, recognized ErM fungi include a narrow taxonomic range within the Ascomycota, and the Sebacinales, basal Hymenomycetes with unclamped hyphae and imperforate parenthesomes. Here we describe a novel type of basidiomycetous ErM symbiosis, termed 'sheathed ericoid mycorrhiza', discovered in two habitats in mid-Norway as a co-dominant mycorrhizal symbiosis in Vaccinium spp. The basidiomycete forming sheathed ErM possesses clamped hyphae with perforate parenthesomes, produces 1- to 3-layer sheaths around terminal parts of hair roots and colonizes their rhizodermis intracellularly forming hyphal coils typical for ErM symbiosis. Two basidiomycetous isolates were obtained from sheathed ErM and molecular and phylogenetic tools were used to determine their identity; they were also examined for the ability to form sheathed ErM and lignocellulolytic potential. Surprisingly, ITS rDNA of both conspecific isolates failed to amplify with the most commonly used primer pairs, including ITS1 and ITS1F + ITS4. Phylogenetic analysis of nuclear LSU, SSU and 5.8S rDNA indicates that the basidiomycete occupies a long branch residing in the proximity of Trechisporales and Hymenochaetales, but lacks a clear sequence relationship (>90% similarity) to fungi currently placed in these orders. The basidiomycete formed the characteristic sheathed ErM symbiosis and enhanced growth of Vaccinium spp. in vitro, and degraded a recalcitrant aromatic substrate that was left unaltered by common ErM ascomycetes. Our findings provide coherent evidence that this hitherto undescribed basidiomycete forms a morphologically distinct ErM symbiosis that may occur at significant levels under natural conditions, yet remain undetected when subject to amplification by 'universal' primers. The lignocellulolytic assay suggests the basidiomycete may confer host adaptations distinct from those provisioned by the so far investigated ascomycetous ErM fungi.
DOI: 10.1371/journal.pone.0039524
PubMed: 22761814
PubMed Central: PMC3382583
Links to Exploration step
pubmed:22761814Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Novel root-fungus symbiosis in Ericaceae: sheathed ericoid mycorrhiza formed by a hitherto undescribed basidiomycete with affinities to Trechisporales.</title><author><name sortKey="Vohnik, Martin" sort="Vohnik, Martin" uniqKey="Vohnik M" first="Martin" last="Vohník">Martin Vohník</name><affiliation><nlm:affiliation>Department of Mycorrhizal Symbioses, Institute of Botany, Academy of Sciences of the Czech Republic, Průhonice, Czech Republic. martin.vohnik@ibot.cas.cz</nlm:affiliation></affiliation></author><author><name sortKey="Sadowsky, Jesse J" sort="Sadowsky, Jesse J" uniqKey="Sadowsky J" first="Jesse J" last="Sadowsky">Jesse J. Sadowsky</name></author><author><name sortKey="Kohout, Petr" sort="Kohout, Petr" uniqKey="Kohout P" first="Petr" last="Kohout">Petr Kohout</name></author><author><name sortKey="Lhotakova, Zuzana" sort="Lhotakova, Zuzana" uniqKey="Lhotakova Z" first="Zuzana" last="Lhotáková">Zuzana Lhotáková</name></author><author><name sortKey="Nestby, Rolf" sort="Nestby, Rolf" uniqKey="Nestby R" first="Rolf" last="Nestby">Rolf Nestby</name></author><author><name sortKey="Kola K, Miroslav" sort="Kola K, Miroslav" uniqKey="Kola K M" first="Miroslav" last="Kola K">Miroslav Kola K</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22761814</idno><idno type="pmid">22761814</idno><idno type="doi">10.1371/journal.pone.0039524</idno><idno type="pmc">PMC3382583</idno><idno type="wicri:Area/Main/Corpus">001F24</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001F24</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Novel root-fungus symbiosis in Ericaceae: sheathed ericoid mycorrhiza formed by a hitherto undescribed basidiomycete with affinities to Trechisporales.</title><author><name sortKey="Vohnik, Martin" sort="Vohnik, Martin" uniqKey="Vohnik M" first="Martin" last="Vohník">Martin Vohník</name><affiliation><nlm:affiliation>Department of Mycorrhizal Symbioses, Institute of Botany, Academy of Sciences of the Czech Republic, Průhonice, Czech Republic. martin.vohnik@ibot.cas.cz</nlm:affiliation></affiliation></author><author><name sortKey="Sadowsky, Jesse J" sort="Sadowsky, Jesse J" uniqKey="Sadowsky J" first="Jesse J" last="Sadowsky">Jesse J. Sadowsky</name></author><author><name sortKey="Kohout, Petr" sort="Kohout, Petr" uniqKey="Kohout P" first="Petr" last="Kohout">Petr Kohout</name></author><author><name sortKey="Lhotakova, Zuzana" sort="Lhotakova, Zuzana" uniqKey="Lhotakova Z" first="Zuzana" last="Lhotáková">Zuzana Lhotáková</name></author><author><name sortKey="Nestby, Rolf" sort="Nestby, Rolf" uniqKey="Nestby R" first="Rolf" last="Nestby">Rolf Nestby</name></author><author><name sortKey="Kola K, Miroslav" sort="Kola K, Miroslav" uniqKey="Kola K M" first="Miroslav" last="Kola K">Miroslav Kola K</name></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term><term>Ericaceae (microbiology)</term><term>Mycorrhizae (classification)</term><term>Mycorrhizae (genetics)</term><term>Norway (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Roots (microbiology)</term><term>Symbiosis (genetics)</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Norway</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term><term>Mycorrhizae</term><term>Symbiosis</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Ericaceae</term><term>Plant Roots</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Phylogeny</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ericaceae (the heath family) are widely distributed calcifuges inhabiting soils with inherently poor nutrient status. Ericaceae overcome nutrient limitation through symbiosis with ericoid mycorrhizal (ErM) fungi that mobilize nutrients complexed in recalcitrant organic matter. At present, recognized ErM fungi include a narrow taxonomic range within the Ascomycota, and the Sebacinales, basal Hymenomycetes with unclamped hyphae and imperforate parenthesomes. Here we describe a novel type of basidiomycetous ErM symbiosis, termed 'sheathed ericoid mycorrhiza', discovered in two habitats in mid-Norway as a co-dominant mycorrhizal symbiosis in Vaccinium spp. The basidiomycete forming sheathed ErM possesses clamped hyphae with perforate parenthesomes, produces 1- to 3-layer sheaths around terminal parts of hair roots and colonizes their rhizodermis intracellularly forming hyphal coils typical for ErM symbiosis. Two basidiomycetous isolates were obtained from sheathed ErM and molecular and phylogenetic tools were used to determine their identity; they were also examined for the ability to form sheathed ErM and lignocellulolytic potential. Surprisingly, ITS rDNA of both conspecific isolates failed to amplify with the most commonly used primer pairs, including ITS1 and ITS1F + ITS4. Phylogenetic analysis of nuclear LSU, SSU and 5.8S rDNA indicates that the basidiomycete occupies a long branch residing in the proximity of Trechisporales and Hymenochaetales, but lacks a clear sequence relationship (>90% similarity) to fungi currently placed in these orders. The basidiomycete formed the characteristic sheathed ErM symbiosis and enhanced growth of Vaccinium spp. in vitro, and degraded a recalcitrant aromatic substrate that was left unaltered by common ErM ascomycetes. Our findings provide coherent evidence that this hitherto undescribed basidiomycete forms a morphologically distinct ErM symbiosis that may occur at significant levels under natural conditions, yet remain undetected when subject to amplification by 'universal' primers. The lignocellulolytic assay suggests the basidiomycete may confer host adaptations distinct from those provisioned by the so far investigated ascomycetous ErM fungi.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22761814</PMID><DateCompleted><Year>2013</Year><Month>03</Month><Day>21</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>6</Issue><PubDate><Year>2012</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Novel root-fungus symbiosis in Ericaceae: sheathed ericoid mycorrhiza formed by a hitherto undescribed basidiomycete with affinities to Trechisporales.</ArticleTitle><Pagination><MedlinePgn>e39524</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0039524</ELocationID><Abstract><AbstractText>Ericaceae (the heath family) are widely distributed calcifuges inhabiting soils with inherently poor nutrient status. Ericaceae overcome nutrient limitation through symbiosis with ericoid mycorrhizal (ErM) fungi that mobilize nutrients complexed in recalcitrant organic matter. At present, recognized ErM fungi include a narrow taxonomic range within the Ascomycota, and the Sebacinales, basal Hymenomycetes with unclamped hyphae and imperforate parenthesomes. Here we describe a novel type of basidiomycetous ErM symbiosis, termed 'sheathed ericoid mycorrhiza', discovered in two habitats in mid-Norway as a co-dominant mycorrhizal symbiosis in Vaccinium spp. The basidiomycete forming sheathed ErM possesses clamped hyphae with perforate parenthesomes, produces 1- to 3-layer sheaths around terminal parts of hair roots and colonizes their rhizodermis intracellularly forming hyphal coils typical for ErM symbiosis. Two basidiomycetous isolates were obtained from sheathed ErM and molecular and phylogenetic tools were used to determine their identity; they were also examined for the ability to form sheathed ErM and lignocellulolytic potential. Surprisingly, ITS rDNA of both conspecific isolates failed to amplify with the most commonly used primer pairs, including ITS1 and ITS1F + ITS4. Phylogenetic analysis of nuclear LSU, SSU and 5.8S rDNA indicates that the basidiomycete occupies a long branch residing in the proximity of Trechisporales and Hymenochaetales, but lacks a clear sequence relationship (>90% similarity) to fungi currently placed in these orders. The basidiomycete formed the characteristic sheathed ErM symbiosis and enhanced growth of Vaccinium spp. in vitro, and degraded a recalcitrant aromatic substrate that was left unaltered by common ErM ascomycetes. Our findings provide coherent evidence that this hitherto undescribed basidiomycete forms a morphologically distinct ErM symbiosis that may occur at significant levels under natural conditions, yet remain undetected when subject to amplification by 'universal' primers. The lignocellulolytic assay suggests the basidiomycete may confer host adaptations distinct from those provisioned by the so far investigated ascomycetous ErM fungi.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vohník</LastName><ForeName>Martin</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Mycorrhizal Symbioses, Institute of Botany, Academy of Sciences of the Czech Republic, Průhonice, Czech Republic. martin.vohnik@ibot.cas.cz</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sadowsky</LastName><ForeName>Jesse J</ForeName><Initials>JJ</Initials></Author><Author ValidYN="Y"><LastName>Kohout</LastName><ForeName>Petr</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Lhotáková</LastName><ForeName>Zuzana</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Nestby</LastName><ForeName>Rolf</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Kolařík</LastName><ForeName>Miroslav</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>06</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029791" MajorTopicYN="N">Ericaceae</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009664" MajorTopicYN="N" Type="Geographic">Norway</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate 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