The Medicago truncatula sucrose transporter family: characterization and implication of key members in carbon partitioning towards arbuscular mycorrhizal fungi.
Identifieur interne : 001E76 ( Main/Corpus ); précédent : 001E75; suivant : 001E77The Medicago truncatula sucrose transporter family: characterization and implication of key members in carbon partitioning towards arbuscular mycorrhizal fungi.
Auteurs : Joan Doidy ; Diederik Van Tuinen ; Olivier Lamotte ; Marion Corneillat ; Gérard Alcaraz ; Daniel WipfSource :
- Molecular plant [ 1752-9867 ] ; 2012.
English descriptors
- KwdEn :
- Carbon (metabolism), Gene Expression Regulation, Plant (drug effects), Glomeromycota (physiology), Medicago truncatula (genetics), Medicago truncatula (growth & development), Medicago truncatula (metabolism), Medicago truncatula (microbiology), Membrane Transport Proteins (genetics), Membrane Transport Proteins (metabolism), Molecular Sequence Data (MeSH), Mycorrhizae (physiology), Phosphates (pharmacology), Plant Proteins (genetics), Plant Proteins (metabolism), Symbiosis (MeSH).
- MESH :
- chemical , genetics : Membrane Transport Proteins, Plant Proteins.
- chemical , metabolism : Carbon, Membrane Transport Proteins, Plant Proteins.
- drug effects : Gene Expression Regulation, Plant.
- genetics : Medicago truncatula.
- growth & development : Medicago truncatula.
- metabolism : Medicago truncatula.
- microbiology : Medicago truncatula.
- chemical , pharmacology : Phosphates.
- physiology : Glomeromycota, Mycorrhizae.
- Molecular Sequence Data, Symbiosis.
Abstract
We identified de novo sucrose transporter (SUT) genes involved in long-distance transport of sucrose from photosynthetic source leaves towards sink organs in the model leguminous species Medicago truncatula. The identification and functional analysis of sugar transporters provide key information on mechanisms that underlie carbon partitioning in plant-microorganism interactions. In that way, full-length sequences of the M. truncatula SUT (MtSUT) family were retrieved and biochemical characterization of MtSUT members was performed by heterologous expression in yeast. The MtSUT family now comprises six genes which distribute among Dicotyledonous clades. MtSUT1-1 and MtSUT4-1 are key members in regard to their expression profiles in source leaves and sink roots and were characterized as functional H(+)/sucrose transporters. Physiological and molecular responses to phosphorus supply and inoculation by the arbuscular mycorrhizal fungus (AMF) Glomus intraradices was studied by gene expression and sugar quantification analyses. Sucrose represents the main sugar transport form in M. truncatula and the expression profiles of MtSUT1-1, MtSUT2, and MtSUT4-1 highlight a fine-tuning regulation for beneficial sugar fluxes towards the fungal symbiont. Taken together, these results suggest distinct functions for proteins from the SUT1, SUT2, and SUT4 clades in plant and in biotrophic interactions.
DOI: 10.1093/mp/sss079
PubMed: 22930732
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last="Corneillat">Marion Corneillat</name></author><author><name sortKey="Alcaraz, Gerard" sort="Alcaraz, Gerard" uniqKey="Alcaraz G" first="Gérard" last="Alcaraz">Gérard Alcaraz</name></author><author><name sortKey="Wipf, Daniel" sort="Wipf, Daniel" uniqKey="Wipf D" first="Daniel" last="Wipf">Daniel Wipf</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22930732</idno><idno type="pmid">22930732</idno><idno type="doi">10.1093/mp/sss079</idno><idno type="wicri:Area/Main/Corpus">001E76</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001E76</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The Medicago truncatula sucrose transporter family: characterization and implication of key members in carbon partitioning towards arbuscular mycorrhizal fungi.</title><author><name sortKey="Doidy, Joan" sort="Doidy, Joan" uniqKey="Doidy J" first="Joan" last="Doidy">Joan Doidy</name><affiliation><nlm:affiliation>UMR INRA 1347, Agrosup, Université de Bourgogne, Agroécologie, Pôle Interactions Plantes Microorganismes ERL CNRS 6300, BP 86510, 21065 Dijon Cedex, France.</nlm:affiliation></affiliation></author><author><name sortKey="Van Tuinen, Diederik" sort="Van Tuinen, Diederik" uniqKey="Van Tuinen D" first="Diederik" last="Van Tuinen">Diederik Van Tuinen</name></author><author><name sortKey="Lamotte, Olivier" sort="Lamotte, Olivier" uniqKey="Lamotte O" first="Olivier" last="Lamotte">Olivier Lamotte</name></author><author><name sortKey="Corneillat, Marion" sort="Corneillat, Marion" uniqKey="Corneillat M" first="Marion" last="Corneillat">Marion Corneillat</name></author><author><name sortKey="Alcaraz, Gerard" sort="Alcaraz, Gerard" uniqKey="Alcaraz G" first="Gérard" last="Alcaraz">Gérard Alcaraz</name></author><author><name sortKey="Wipf, Daniel" sort="Wipf, Daniel" uniqKey="Wipf D" first="Daniel" last="Wipf">Daniel Wipf</name></author></analytic><series><title level="j">Molecular plant</title><idno type="eISSN">1752-9867</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbon (metabolism)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Glomeromycota (physiology)</term><term>Medicago truncatula (genetics)</term><term>Medicago truncatula (growth & development)</term><term>Medicago truncatula (metabolism)</term><term>Medicago truncatula (microbiology)</term><term>Membrane Transport Proteins (genetics)</term><term>Membrane Transport Proteins (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (physiology)</term><term>Phosphates (pharmacology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Transport Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon</term><term>Membrane Transport Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation, Plant</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Medicago truncatula</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Medicago truncatula</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Medicago truncatula</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Medicago truncatula</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Phosphates</term></keywords><keywords scheme="MESH" qualifier="physiology" 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The identification and functional analysis of sugar transporters provide key information on mechanisms that underlie carbon partitioning in plant-microorganism interactions. In that way, full-length sequences of the M. truncatula SUT (MtSUT) family were retrieved and biochemical characterization of MtSUT members was performed by heterologous expression in yeast. The MtSUT family now comprises six genes which distribute among Dicotyledonous clades. MtSUT1-1 and MtSUT4-1 are key members in regard to their expression profiles in source leaves and sink roots and were characterized as functional H(+)/sucrose transporters. Physiological and molecular responses to phosphorus supply and inoculation by the arbuscular mycorrhizal fungus (AMF) Glomus intraradices was studied by gene expression and sugar quantification analyses. Sucrose represents the main sugar transport form in M. truncatula and the expression profiles of MtSUT1-1, MtSUT2, and MtSUT4-1 highlight a fine-tuning regulation for beneficial sugar fluxes towards the fungal symbiont. Taken together, these results suggest distinct functions for proteins from the SUT1, SUT2, and SUT4 clades in plant and in biotrophic interactions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22930732</PMID><DateCompleted><Year>2013</Year><Month>11</Month><Day>25</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>27</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1752-9867</ISSN><JournalIssue CitedMedium="Internet"><Volume>5</Volume><Issue>6</Issue><PubDate><Year>2012</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Molecular plant</Title><ISOAbbreviation>Mol Plant</ISOAbbreviation></Journal><ArticleTitle>The Medicago truncatula sucrose transporter family: characterization and implication of key members in carbon partitioning towards arbuscular mycorrhizal fungi.</ArticleTitle><Pagination><MedlinePgn>1346-58</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/mp/sss079</ELocationID><Abstract><AbstractText>We identified de novo sucrose transporter (SUT) genes involved in long-distance transport of sucrose from photosynthetic source leaves towards sink organs in the model leguminous species Medicago truncatula. The identification and functional analysis of sugar transporters provide key information on mechanisms that underlie carbon partitioning in plant-microorganism interactions. In that way, full-length sequences of the M. truncatula SUT (MtSUT) family were retrieved and biochemical characterization of MtSUT members was performed by heterologous expression in yeast. The MtSUT family now comprises six genes which distribute among Dicotyledonous clades. MtSUT1-1 and MtSUT4-1 are key members in regard to their expression profiles in source leaves and sink roots and were characterized as functional H(+)/sucrose transporters. Physiological and molecular responses to phosphorus supply and inoculation by the arbuscular mycorrhizal fungus (AMF) Glomus intraradices was studied by gene expression and sugar quantification analyses. Sucrose represents the main sugar transport form in M. truncatula and the expression profiles of MtSUT1-1, MtSUT2, and MtSUT4-1 highlight a fine-tuning regulation for beneficial sugar fluxes towards the fungal symbiont. 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