MycorrhizaeV1, Main, Corpus, bibRecord, 001E39

An AM-induced, MYB-family gene of Lotus japonicus (LjMAMI) affects root growth in an AM-independent manner.

Identifieur interne : 001E39 ( Main/Corpus ); précédent : 001E38; suivant : 001E40

An AM-induced, MYB-family gene of Lotus japonicus (LjMAMI) affects root growth in an AM-independent manner.

Auteurs : Veronica Volpe ; Elisa Dell'Aglio ; Marco Giovannetti ; Cristina Ruberti ; Alex Costa ; Andrea Genre ; Mike Guether ; Paola Bonfante

Source :

The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2013.

RBID : pubmed:23051146

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Genes, Plant (MeSH), Genes, myb (MeSH), Lotus (genetics), Molecular Sequence Data (MeSH), Mycorrhizae (physiology), Plant Roots (growth & development), Reverse Transcriptase Polymerase Chain Reaction (MeSH).
MESH :
- genetics : Lotus.
- growth & development : Plant Roots.
- physiology : Mycorrhizae.
- Amino Acid Sequence, Genes, Plant, Genes, myb, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction.

Abstract

The interaction between legumes and arbuscular mycorrhizal (AM) fungi is vital to the development of sustainable plant production systems. Here, we focus on a putative MYB-like (LjMAMI) transcription factor (TF) previously reported to be highly upregulated in Lotus japonicus mycorrhizal roots. Phylogenetic analyses revealed that the protein is related to a group of TFs involved in phosphate (Pi) starvation responses, the expression of which is independent of the Pi level, such as PHR1. GUS transformed plants and quantitative reverse transcription PCR revealed strong gene induction in arbusculated cells, as well as the presence of LjMAMI transcripts in lateral root primordia and root meristems, even in the absence of the fungus, and independently of Pi concentration. In agreement with its putative identification as a TF, an eGFP-LjMAMI chimera was localized to the nuclei of plant protoplasts, whereas in transgenic Lotus roots expressing the eGFP-LjMAMI fusion protein under the control of the native promoter, the protein was located in the nuclei of the arbusculated cells. Further expression analyses revealed a correlation between LjMAMI and LjPT4, a marker gene for mycorrhizal function. To elucidate the role of the LjMAMI gene in the mycorrhizal process, RNAi and overexpressing root lines were generated. All the lines retained their symbiotic capacity; however, RNAi root lines and composite plants showed an important reduction in root elongation and branching in the absence of the symbiont. The results support the involvement of the AM-responsive LjMAMI in non-symbiotic functions: i.e. root growth.

DOI: 10.1111/tpj.12045
PubMed: 23051146

Links to Exploration step

pubmed:23051146

Le document en format XML

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<author><name sortKey="Volpe, Veronica" sort="Volpe, Veronica" uniqKey="Volpe V" first="Veronica" last="Volpe">Veronica Volpe</name>
<affiliation><nlm:affiliation>Department of Life Sciences and Systems Biology, University of Torino, Viale Mattioli 25, I-10125, Torino, Italy.</nlm:affiliation>
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<author><name sortKey="Dell Aglio, Elisa" sort="Dell Aglio, Elisa" uniqKey="Dell Aglio E" first="Elisa" last="Dell'Aglio">Elisa Dell'Aglio</name>
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<author><name sortKey="Giovannetti, Marco" sort="Giovannetti, Marco" uniqKey="Giovannetti M" first="Marco" last="Giovannetti">Marco Giovannetti</name>
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<author><name sortKey="Ruberti, Cristina" sort="Ruberti, Cristina" uniqKey="Ruberti C" first="Cristina" last="Ruberti">Cristina Ruberti</name>
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<author><name sortKey="Costa, Alex" sort="Costa, Alex" uniqKey="Costa A" first="Alex" last="Costa">Alex Costa</name>
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<author><name sortKey="Genre, Andrea" sort="Genre, Andrea" uniqKey="Genre A" first="Andrea" last="Genre">Andrea Genre</name>
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<author><name sortKey="Guether, Mike" sort="Guether, Mike" uniqKey="Guether M" first="Mike" last="Guether">Mike Guether</name>
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<author><name sortKey="Bonfante, Paola" sort="Bonfante, Paola" uniqKey="Bonfante P" first="Paola" last="Bonfante">Paola Bonfante</name>
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<author><name sortKey="Giovannetti, Marco" sort="Giovannetti, Marco" uniqKey="Giovannetti M" first="Marco" last="Giovannetti">Marco Giovannetti</name>
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<term>Lotus (genetics)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Mycorrhizae (physiology)</term>
<term>Plant Roots (growth & development)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term>
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<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
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<front><div type="abstract" xml:lang="en">The interaction between legumes and arbuscular mycorrhizal (AM) fungi is vital to the development of sustainable plant production systems. Here, we focus on a putative MYB-like (LjMAMI) transcription factor (TF) previously reported to be highly upregulated in Lotus japonicus mycorrhizal roots. Phylogenetic analyses revealed that the protein is related to a group of TFs involved in phosphate (Pi) starvation responses, the expression of which is independent of the Pi level, such as PHR1. GUS transformed plants and quantitative reverse transcription PCR revealed strong gene induction in arbusculated cells, as well as the presence of LjMAMI transcripts in lateral root primordia and root meristems, even in the absence of the fungus, and independently of Pi concentration. In agreement with its putative identification as a TF, an eGFP-LjMAMI chimera was localized to the nuclei of plant protoplasts, whereas in transgenic Lotus roots expressing the eGFP-LjMAMI fusion protein under the control of the native promoter, the protein was located in the nuclei of the arbusculated cells. Further expression analyses revealed a correlation between LjMAMI and LjPT4, a marker gene for mycorrhizal function. To elucidate the role of the LjMAMI gene in the mycorrhizal process, RNAi and overexpressing root lines were generated. All the lines retained their symbiotic capacity; however, RNAi root lines and composite plants showed an important reduction in root elongation and branching in the absence of the symbiont. The results support the involvement of the AM-responsive LjMAMI in non-symbiotic functions: i.e. root growth.</div>
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<ArticleTitle>An AM-induced, MYB-family gene of Lotus japonicus (LjMAMI) affects root growth in an AM-independent manner.</ArticleTitle>
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<Abstract><AbstractText>The interaction between legumes and arbuscular mycorrhizal (AM) fungi is vital to the development of sustainable plant production systems. Here, we focus on a putative MYB-like (LjMAMI) transcription factor (TF) previously reported to be highly upregulated in Lotus japonicus mycorrhizal roots. Phylogenetic analyses revealed that the protein is related to a group of TFs involved in phosphate (Pi) starvation responses, the expression of which is independent of the Pi level, such as PHR1. GUS transformed plants and quantitative reverse transcription PCR revealed strong gene induction in arbusculated cells, as well as the presence of LjMAMI transcripts in lateral root primordia and root meristems, even in the absence of the fungus, and independently of Pi concentration. In agreement with its putative identification as a TF, an eGFP-LjMAMI chimera was localized to the nuclei of plant protoplasts, whereas in transgenic Lotus roots expressing the eGFP-LjMAMI fusion protein under the control of the native promoter, the protein was located in the nuclei of the arbusculated cells. Further expression analyses revealed a correlation between LjMAMI and LjPT4, a marker gene for mycorrhizal function. To elucidate the role of the LjMAMI gene in the mycorrhizal process, RNAi and overexpressing root lines were generated. All the lines retained their symbiotic capacity; however, RNAi root lines and composite plants showed an important reduction in root elongation and branching in the absence of the symbiont. The results support the involvement of the AM-responsive LjMAMI in non-symbiotic functions: i.e. root growth.</AbstractText>
<CopyrightInformation>© 2012 The Authors The Plant Journal © 2012 Blackwell Publishing Ltd.</CopyrightInformation>
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An AM-induced, MYB-family gene of Lotus japonicus (LjMAMI) affects root growth in an AM-independent manner.

An AM-induced, MYB-family gene of Lotus japonicus (LjMAMI) affects root growth in an AM-independent manner.

Source :

English descriptors

Abstract

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