The interplay between P uptake pathways in mycorrhizal peas: a combined physiological and gene-silencing approach.
Identifieur interne : 001D25 ( Main/Corpus ); précédent : 001D24; suivant : 001D26The interplay between P uptake pathways in mycorrhizal peas: a combined physiological and gene-silencing approach.
Auteurs : Mette Gr Nlund ; Merete Albrechtsen ; I Elisabeth Johansen ; Edith C. Hammer ; Tom H. Nielsen ; Iver JakobsenSource :
- Physiologia plantarum [ 1399-3054 ] ; 2013.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Biological Transport (physiology), Gene Silencing (MeSH), Host-Pathogen Interactions (MeSH), Molecular Sequence Data (MeSH), Mycorrhizae (physiology), Peas (genetics), Peas (microbiology), Peas (physiology), Phosphate Transport Proteins (classification), Phosphate Transport Proteins (genetics), Phosphate Transport Proteins (physiology), Phosphates (metabolism), Phosphorus (metabolism), Phosphorus Radioisotopes (metabolism), Phylogeny (MeSH), Plant Proteins (genetics), Plant Proteins (physiology), Plant Roots (genetics), Plant Roots (microbiology), Plant Roots (physiology), Sequence Homology, Amino Acid (MeSH), Signal Transduction (genetics), Signal Transduction (physiology), Soil (chemistry), Symbiosis (genetics), Symbiosis (physiology).
- MESH :
- chemical , chemistry : Soil.
- chemical , classification : Phosphate Transport Proteins.
- genetics : Peas, Phosphate Transport Proteins, Plant Proteins, Plant Roots, Signal Transduction, Symbiosis.
- chemical , metabolism : Phosphates, Phosphorus, Phosphorus Radioisotopes.
- microbiology : Peas, Plant Roots.
- physiology : Biological Transport, Mycorrhizae, Peas, Phosphate Transport Proteins, Plant Proteins, Plant Roots, Signal Transduction, Symbiosis.
- Amino Acid Sequence, Gene Silencing, Host-Pathogen Interactions, Molecular Sequence Data, Phylogeny, Sequence Homology, Amino Acid.
Abstract
Arbuscular mycorrhizal fungi (AMF) have a key role in plant phosphate (Pi) uptake by their efficient capture of soil phosphorus (P) that is transferred to the plant via Pi transporters in the root cortical cells. The activity of this mycorrhizal Pi uptake pathway is often associated with downregulation of Pi transporter genes in the direct Pi uptake pathway. As the total Pi taken up by the plant is determined by the combined activity of mycorrhizal and direct pathways, it is important to understand the interplay between these, in particular the actual activity of the pathways. To study this interplay we modulated the delivery of Pi via the mycorrhizal pathway in Pisum sativum by two means: (1) Partial downregulation by virus-induced gene silencing of PsPT4, a putative Pi transporter gene in the mycorrhizal pathway. This resulted in decreased fungal development in roots and soil and led to reduced plant Pi uptake. (2) Changing the percentage of AMF-colonized root length by using non-, half-mycorrhizal or full-mycorrhizal split-root systems. The combination of split roots, use of ³²P and ³³P isotopes and partial silencing of PsPT4 enabled us to show that the expression of PsPT1, a putative Pi transporter gene in the direct pathway, was negatively correlated with increasing mycorrhizal uptake capacity of the plant, both locally and systemically. However, transcript changes in PsPT1 were not translated into corresponding, systemic changes in actual direct Pi uptake. Our results suggest that AMF have a limited long-distance impact on the direct pathway.
DOI: 10.1111/ppl.12030
PubMed: 23387980
Links to Exploration step
pubmed:23387980Le document en format XML
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Hammer</name></author><author><name sortKey="Nielsen, Tom H" sort="Nielsen, Tom H" uniqKey="Nielsen T" first="Tom H" last="Nielsen">Tom H. Nielsen</name></author><author><name sortKey="Jakobsen, Iver" sort="Jakobsen, Iver" uniqKey="Jakobsen I" first="Iver" last="Jakobsen">Iver Jakobsen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23387980</idno><idno type="pmid">23387980</idno><idno type="doi">10.1111/ppl.12030</idno><idno type="wicri:Area/Main/Corpus">001D25</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001D25</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The interplay between P uptake pathways in mycorrhizal peas: a combined physiological and gene-silencing approach.</title><author><name sortKey="Gr Nlund, Mette" sort="Gr Nlund, Mette" uniqKey="Gr Nlund M" first="Mette" last="Gr Nlund">Mette Gr Nlund</name><affiliation><nlm:affiliation>Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark.</nlm:affiliation></affiliation></author><author><name sortKey="Albrechtsen, Merete" sort="Albrechtsen, Merete" uniqKey="Albrechtsen M" first="Merete" last="Albrechtsen">Merete Albrechtsen</name></author><author><name sortKey="Johansen, I Elisabeth" sort="Johansen, I Elisabeth" uniqKey="Johansen I" first="I Elisabeth" last="Johansen">I Elisabeth Johansen</name></author><author><name sortKey="Hammer, Edith C" sort="Hammer, Edith C" uniqKey="Hammer E" first="Edith C" last="Hammer">Edith C. Hammer</name></author><author><name sortKey="Nielsen, Tom H" sort="Nielsen, Tom H" uniqKey="Nielsen T" first="Tom H" last="Nielsen">Tom H. Nielsen</name></author><author><name sortKey="Jakobsen, Iver" sort="Jakobsen, Iver" uniqKey="Jakobsen I" first="Iver" last="Jakobsen">Iver Jakobsen</name></author></analytic><series><title level="j">Physiologia plantarum</title><idno type="eISSN">1399-3054</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Biological Transport (physiology)</term><term>Gene Silencing (MeSH)</term><term>Host-Pathogen Interactions (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (physiology)</term><term>Peas (genetics)</term><term>Peas (microbiology)</term><term>Peas (physiology)</term><term>Phosphate Transport Proteins (classification)</term><term>Phosphate Transport Proteins (genetics)</term><term>Phosphate Transport Proteins (physiology)</term><term>Phosphates (metabolism)</term><term>Phosphorus (metabolism)</term><term>Phosphorus Radioisotopes (metabolism)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (physiology)</term><term>Plant Roots (genetics)</term><term>Plant Roots (microbiology)</term><term>Plant Roots (physiology)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Signal Transduction (genetics)</term><term>Signal Transduction (physiology)</term><term>Soil (chemistry)</term><term>Symbiosis (genetics)</term><term>Symbiosis (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Soil</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Phosphate Transport Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Peas</term><term>Phosphate Transport Proteins</term><term>Plant Proteins</term><term>Plant Roots</term><term>Signal Transduction</term><term>Symbiosis</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Phosphates</term><term>Phosphorus</term><term>Phosphorus Radioisotopes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Peas</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Biological Transport</term><term>Mycorrhizae</term><term>Peas</term><term>Phosphate Transport Proteins</term><term>Plant Proteins</term><term>Plant Roots</term><term>Signal Transduction</term><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Gene Silencing</term><term>Host-Pathogen Interactions</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Homology, Amino Acid</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Arbuscular mycorrhizal fungi (AMF) have a key role in plant phosphate (Pi) uptake by their efficient capture of soil phosphorus (P) that is transferred to the plant via Pi transporters in the root cortical cells. The activity of this mycorrhizal Pi uptake pathway is often associated with downregulation of Pi transporter genes in the direct Pi uptake pathway. As the total Pi taken up by the plant is determined by the combined activity of mycorrhizal and direct pathways, it is important to understand the interplay between these, in particular the actual activity of the pathways. To study this interplay we modulated the delivery of Pi via the mycorrhizal pathway in Pisum sativum by two means: (1) Partial downregulation by virus-induced gene silencing of PsPT4, a putative Pi transporter gene in the mycorrhizal pathway. This resulted in decreased fungal development in roots and soil and led to reduced plant Pi uptake. (2) Changing the percentage of AMF-colonized root length by using non-, half-mycorrhizal or full-mycorrhizal split-root systems. The combination of split roots, use of ³²P and ³³P isotopes and partial silencing of PsPT4 enabled us to show that the expression of PsPT1, a putative Pi transporter gene in the direct pathway, was negatively correlated with increasing mycorrhizal uptake capacity of the plant, both locally and systemically. However, transcript changes in PsPT1 were not translated into corresponding, systemic changes in actual direct Pi uptake. Our results suggest that AMF have a limited long-distance impact on the direct pathway.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23387980</PMID><DateCompleted><Year>2014</Year><Month>04</Month><Day>21</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1399-3054</ISSN><JournalIssue CitedMedium="Internet"><Volume>149</Volume><Issue>2</Issue><PubDate><Year>2013</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Physiologia plantarum</Title><ISOAbbreviation>Physiol Plant</ISOAbbreviation></Journal><ArticleTitle>The interplay between P uptake pathways in mycorrhizal peas: a combined physiological and gene-silencing approach.</ArticleTitle><Pagination><MedlinePgn>234-48</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/ppl.12030</ELocationID><Abstract><AbstractText>Arbuscular mycorrhizal fungi (AMF) have a key role in plant phosphate (Pi) uptake by their efficient capture of soil phosphorus (P) that is transferred to the plant via Pi transporters in the root cortical cells. The activity of this mycorrhizal Pi uptake pathway is often associated with downregulation of Pi transporter genes in the direct Pi uptake pathway. As the total Pi taken up by the plant is determined by the combined activity of mycorrhizal and direct pathways, it is important to understand the interplay between these, in particular the actual activity of the pathways. To study this interplay we modulated the delivery of Pi via the mycorrhizal pathway in Pisum sativum by two means: (1) Partial downregulation by virus-induced gene silencing of PsPT4, a putative Pi transporter gene in the mycorrhizal pathway. This resulted in decreased fungal development in roots and soil and led to reduced plant Pi uptake. (2) Changing the percentage of AMF-colonized root length by using non-, half-mycorrhizal or full-mycorrhizal split-root systems. The combination of split roots, use of ³²P and ³³P isotopes and partial silencing of PsPT4 enabled us to show that the expression of PsPT1, a putative Pi transporter gene in the direct pathway, was negatively correlated with increasing mycorrhizal uptake capacity of the plant, both locally and systemically. However, transcript changes in PsPT1 were not translated into corresponding, systemic changes in actual direct Pi uptake. Our results suggest that AMF have a limited long-distance impact on the direct pathway.</AbstractText><CopyrightInformation>© 2013 Scandinavian Plant Physiology Society.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Grønlund</LastName><ForeName>Mette</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Albrechtsen</LastName><ForeName>Merete</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Johansen</LastName><ForeName>I Elisabeth</ForeName><Initials>IE</Initials></Author><Author ValidYN="Y"><LastName>Hammer</LastName><ForeName>Edith C</ForeName><Initials>EC</Initials></Author><Author ValidYN="Y"><LastName>Nielsen</LastName><ForeName>Tom H</ForeName><Initials>TH</Initials></Author><Author ValidYN="Y"><LastName>Jakobsen</LastName><ForeName>Iver</ForeName><Initials>I</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>03</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Physiol Plant</MedlineTA><NlmUniqueID>1256322</NlmUniqueID><ISSNLinking>0031-9317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D028061">Phosphate Transport Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010710">Phosphates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010761">Phosphorus Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012987">Soil</NameOfSubstance></Chemical><Chemical><RegistryNumber>27YLU75U4W</RegistryNumber><NameOfSubstance UI="D010758">Phosphorus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001692" MajorTopicYN="N">Biological Transport</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="N">Gene Silencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018532" MajorTopicYN="N">Peas</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028061" MajorTopicYN="N">Phosphate Transport Proteins</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010710" MajorTopicYN="N">Phosphates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010758" MajorTopicYN="N">Phosphorus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010761" MajorTopicYN="N">Phosphorus Radioisotopes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012987" MajorTopicYN="N">Soil</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>12</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>01</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>01</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>2</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>2</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>4</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23387980</ArticleId><ArticleId IdType="doi">10.1111/ppl.12030</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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