Zinc export results in adaptive zinc tolerance in the ectomycorrhizal basidiomycete Suillus bovinus.
Identifieur interne : 001C08 ( Main/Corpus ); précédent : 001C07; suivant : 001C09Zinc export results in adaptive zinc tolerance in the ectomycorrhizal basidiomycete Suillus bovinus.
Auteurs : Joske Ruytinx ; Hoai Nguyen ; May Van Hees ; Michiel Op De Beeck ; Jaco Vangronsveld ; Robert Carleer ; Jan V. Colpaert ; Kristin AdriaensenSource :
- Metallomics : integrated biometal science [ 1756-591X ] ; 2013.
English descriptors
- KwdEn :
- Adaptation, Physiological (MeSH), Basidiomycota (metabolism), Biological Transport (drug effects), Carbonyl Cyanide m-Chlorophenyl Hydrazone (pharmacology), Cell Wall (metabolism), Cytoplasm (metabolism), Kinetics (MeSH), Mycelium (metabolism), Mycorrhizae (metabolism), Proton Ionophores (pharmacology), Soil Pollutants (metabolism), Vacuoles (metabolism), Zinc (metabolism).
- MESH :
- chemical , metabolism : Soil Pollutants, Zinc.
- chemical , pharmacology : Carbonyl Cyanide m-Chlorophenyl Hydrazone, Proton Ionophores.
- drug effects : Biological Transport.
- metabolism : Basidiomycota, Cell Wall, Cytoplasm, Mycelium, Mycorrhizae, Vacuoles.
- Adaptation, Physiological, Kinetics.
Abstract
On Zn-polluted soils, populations of the ectomycorrhizal basidiomycete Suillus bovinus exhibit an elevated Zn tolerance when compared to populations on non-polluted sites. To elucidate the mechanism of Zn tolerance, the time-course of Zn uptake was studied in isolates with contrasting Zn tolerance. Unidirectional fluxes and subcellular compartmentation of Zn were investigated through radiotracer flux analyses. Fluorescence imaging was used to support the subcellular Zn compartmentation. After 2 h of exposure to 200 μM Zn, significantly more Zn was accumulated in Zn-sensitive isolates compared to tolerant isolates, despite similar short-term uptake kinetics and similar extracellular Zn sequestration in cell walls. In Zn-sensitive isolates twice as much Zn accumulated in the cytoplasm and 12 times more Zn in the vacuole. (65)Zn efflux analyses revealed a considerably faster Zn export in the Zn-tolerant isolate. The adaptive Zn tolerance in S. bovinus is therefore achieved by a preferential removal of Zn out of the cytoplasm, back into the apoplast, instead of the usual transfer of Zn into the vacuole. Zn exclusion in the fungal symbiont eventually contributes to a lower Zn influx in host plants.
DOI: 10.1039/c3mt00061c
PubMed: 23715468
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pubmed:23715468Le document en format XML
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Colpaert</name></author><author><name sortKey="Adriaensen, Kristin" sort="Adriaensen, Kristin" uniqKey="Adriaensen K" first="Kristin" last="Adriaensen">Kristin Adriaensen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23715468</idno><idno type="pmid">23715468</idno><idno type="doi">10.1039/c3mt00061c</idno><idno type="wicri:Area/Main/Corpus">001C08</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001C08</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Zinc export results in adaptive zinc tolerance in the ectomycorrhizal basidiomycete Suillus bovinus.</title><author><name sortKey="Ruytinx, Joske" sort="Ruytinx, Joske" uniqKey="Ruytinx J" first="Joske" last="Ruytinx">Joske Ruytinx</name><affiliation><nlm:affiliation>Hasselt University, Centre for Environmental Sciences, Environmental Biology Group, Agoralaan, Gebouw D, 3590 Diepenbeek, Belgium.</nlm:affiliation></affiliation></author><author><name sortKey="Nguyen, Hoai" sort="Nguyen, Hoai" uniqKey="Nguyen H" first="Hoai" last="Nguyen">Hoai Nguyen</name></author><author><name sortKey="Van Hees, May" sort="Van Hees, May" uniqKey="Van Hees M" first="May" last="Van Hees">May Van Hees</name></author><author><name sortKey="Op De Beeck, Michiel" sort="Op De Beeck, Michiel" uniqKey="Op De Beeck M" first="Michiel" last="Op De Beeck">Michiel Op De Beeck</name></author><author><name sortKey="Vangronsveld, Jaco" sort="Vangronsveld, Jaco" uniqKey="Vangronsveld J" first="Jaco" last="Vangronsveld">Jaco Vangronsveld</name></author><author><name sortKey="Carleer, Robert" sort="Carleer, Robert" uniqKey="Carleer R" first="Robert" last="Carleer">Robert Carleer</name></author><author><name sortKey="Colpaert, Jan V" sort="Colpaert, Jan V" uniqKey="Colpaert J" first="Jan V" last="Colpaert">Jan V. Colpaert</name></author><author><name sortKey="Adriaensen, Kristin" sort="Adriaensen, Kristin" uniqKey="Adriaensen K" first="Kristin" last="Adriaensen">Kristin Adriaensen</name></author></analytic><series><title level="j">Metallomics : integrated biometal science</title><idno type="eISSN">1756-591X</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Physiological (MeSH)</term><term>Basidiomycota (metabolism)</term><term>Biological Transport (drug effects)</term><term>Carbonyl Cyanide m-Chlorophenyl Hydrazone (pharmacology)</term><term>Cell Wall (metabolism)</term><term>Cytoplasm (metabolism)</term><term>Kinetics (MeSH)</term><term>Mycelium (metabolism)</term><term>Mycorrhizae (metabolism)</term><term>Proton Ionophores (pharmacology)</term><term>Soil Pollutants (metabolism)</term><term>Vacuoles (metabolism)</term><term>Zinc (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Soil Pollutants</term><term>Zinc</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Carbonyl Cyanide m-Chlorophenyl Hydrazone</term><term>Proton Ionophores</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Biological Transport</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term><term>Cell Wall</term><term>Cytoplasm</term><term>Mycelium</term><term>Mycorrhizae</term><term>Vacuoles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adaptation, Physiological</term><term>Kinetics</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">On Zn-polluted soils, populations of the ectomycorrhizal basidiomycete Suillus bovinus exhibit an elevated Zn tolerance when compared to populations on non-polluted sites. To elucidate the mechanism of Zn tolerance, the time-course of Zn uptake was studied in isolates with contrasting Zn tolerance. Unidirectional fluxes and subcellular compartmentation of Zn were investigated through radiotracer flux analyses. Fluorescence imaging was used to support the subcellular Zn compartmentation. After 2 h of exposure to 200 μM Zn, significantly more Zn was accumulated in Zn-sensitive isolates compared to tolerant isolates, despite similar short-term uptake kinetics and similar extracellular Zn sequestration in cell walls. In Zn-sensitive isolates twice as much Zn accumulated in the cytoplasm and 12 times more Zn in the vacuole. (65)Zn efflux analyses revealed a considerably faster Zn export in the Zn-tolerant isolate. The adaptive Zn tolerance in S. bovinus is therefore achieved by a preferential removal of Zn out of the cytoplasm, back into the apoplast, instead of the usual transfer of Zn into the vacuole. Zn exclusion in the fungal symbiont eventually contributes to a lower Zn influx in host plants. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23715468</PMID><DateCompleted><Year>2014</Year><Month>03</Month><Day>25</Day></DateCompleted><DateRevised><Year>2013</Year><Month>08</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1756-591X</ISSN><JournalIssue CitedMedium="Internet"><Volume>5</Volume><Issue>9</Issue><PubDate><Year>2013</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Metallomics : integrated biometal science</Title><ISOAbbreviation>Metallomics</ISOAbbreviation></Journal><ArticleTitle>Zinc export results in adaptive zinc tolerance in the ectomycorrhizal basidiomycete Suillus bovinus.</ArticleTitle><Pagination><MedlinePgn>1225-33</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1039/c3mt00061c</ELocationID><Abstract><AbstractText>On Zn-polluted soils, populations of the ectomycorrhizal basidiomycete Suillus bovinus exhibit an elevated Zn tolerance when compared to populations on non-polluted sites. To elucidate the mechanism of Zn tolerance, the time-course of Zn uptake was studied in isolates with contrasting Zn tolerance. Unidirectional fluxes and subcellular compartmentation of Zn were investigated through radiotracer flux analyses. Fluorescence imaging was used to support the subcellular Zn compartmentation. After 2 h of exposure to 200 μM Zn, significantly more Zn was accumulated in Zn-sensitive isolates compared to tolerant isolates, despite similar short-term uptake kinetics and similar extracellular Zn sequestration in cell walls. In Zn-sensitive isolates twice as much Zn accumulated in the cytoplasm and 12 times more Zn in the vacuole. (65)Zn efflux analyses revealed a considerably faster Zn export in the Zn-tolerant isolate. The adaptive Zn tolerance in S. bovinus is therefore achieved by a preferential removal of Zn out of the cytoplasm, back into the apoplast, instead of the usual transfer of Zn into the vacuole. Zn exclusion in the fungal symbiont eventually contributes to a lower Zn influx in host plants. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ruytinx</LastName><ForeName>Joske</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Hasselt University, Centre for Environmental Sciences, Environmental Biology Group, Agoralaan, Gebouw D, 3590 Diepenbeek, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nguyen</LastName><ForeName>Hoai</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Van Hees</LastName><ForeName>May</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Op De Beeck</LastName><ForeName>Michiel</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Vangronsveld</LastName><ForeName>Jaco</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Carleer</LastName><ForeName>Robert</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Colpaert</LastName><ForeName>Jan V</ForeName><Initials>JV</Initials></Author><Author ValidYN="Y"><LastName>Adriaensen</LastName><ForeName>Kristin</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Metallomics</MedlineTA><NlmUniqueID>101478346</NlmUniqueID><ISSNLinking>1756-5901</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D061209">Proton Ionophores</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012989">Soil Pollutants</NameOfSubstance></Chemical><Chemical><RegistryNumber>555-60-2</RegistryNumber><NameOfSubstance UI="D002258">Carbonyl Cyanide m-Chlorophenyl Hydrazone</NameOfSubstance></Chemical><Chemical><RegistryNumber>J41CSQ7QDS</RegistryNumber><NameOfSubstance UI="D015032">Zinc</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000222" MajorTopicYN="Y">Adaptation, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001692" MajorTopicYN="N">Biological Transport</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002258" MajorTopicYN="N">Carbonyl Cyanide m-Chlorophenyl Hydrazone</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003593" MajorTopicYN="N">Cytoplasm</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D025282" MajorTopicYN="N">Mycelium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D061209" MajorTopicYN="N">Proton Ionophores</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012989" MajorTopicYN="N">Soil Pollutants</DescriptorName><QualifierName UI="Q000378" 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