Assessment of the applicability of a "toolbox" designed for microbially assisted phytoremediation: the case study at Ingurtosu mining site (Italy).
Identifieur interne : 001A68 ( Main/Corpus ); précédent : 001A67; suivant : 001A69Assessment of the applicability of a "toolbox" designed for microbially assisted phytoremediation: the case study at Ingurtosu mining site (Italy).
Auteurs : Anna Rosa Sprocati ; Chiara Alisi ; Valentina Pinto ; Maria Rita Montereali ; Paola Marconi ; Flavia Tasso ; Katarzyna Turnau ; Giovanni De Giudici ; Katarzyna Goralska ; Marta Bevilacqua ; Federico Marini ; Carlo CremisiniSource :
- Environmental science and pollution research international [ 1614-7499 ] ; 2014.
English descriptors
- KwdEn :
- Bacteria (metabolism), Biodegradation, Environmental (MeSH), Euphorbia (growth & development), Euphorbia (metabolism), Euphorbia (microbiology), Fungi (metabolism), Italy (MeSH), Metals, Heavy (analysis), Mining (MeSH), Multivariate Analysis (MeSH), Mycorrhizae (metabolism), Plant Roots (metabolism), Soil (chemistry), Soil Microbiology (MeSH), Soil Pollutants (analysis).
- MESH :
- chemical , analysis : Metals, Heavy, Soil Pollutants.
- chemical , chemistry : Soil.
- geographic : Italy.
- growth & development : Euphorbia.
- metabolism : Bacteria, Euphorbia, Fungi, Mycorrhizae, Plant Roots.
- microbiology : Euphorbia.
- Biodegradation, Environmental, Mining, Multivariate Analysis, Soil Microbiology.
Abstract
The paper describes the fieldwork at the Italian test site of the abandoned mine of sphalerite and galena in Ingurtosu (Sardinia), with the aim to assess the applicability of a "toolbox" to establish the optimized techniques for remediation of soils contaminated by mining activities. A preliminary characterization-including (hydro)geochemistry, heavy metal concentration and their mobility in soil, bioprospecting for microbiology and botany-provided a data set for the development of a toolbox to deliver a microbially assisted phytoremediation process. Euphorbia pithyusa was selected as an endemic pioneer plant to be associated with a bacterial consortium, established with ten selected native strains, including metal-tolerant bacteria and producers of plant growth factors. The toolbox was firstly assessed in a greenhouse pot experiment. A positive effect of bacterial inoculum on E. pithyusa germination and total plant survival was observed. E. pithyusa showed to be a well-performing metallophyte species, and only inoculated soil retained a microbial activity with a high functional diversity, expanding metabolic affinity also towards root exudates. These results supported the decision to proceed with a field trial, investigating different treatments used singly or in combination: bioaugmentation with bacterial consortia, mycorrhizal fungi and a commercial mineral amendment. Microbial activity in soil, plant physiological parameters and heavy metal content in plants and in soil were monitored. Five months after the beginning, an early assessment of the toolbox under field conditions was carried out. Despite the cold season (October-March), results suggested the following: (1) the field setup as well as the experimental design proved to be effective; (2) plant survival was satisfactory; (3) soil quality was increased and bioaugmentation improved microbial activity, expanding the metabolic competences towards plant interaction (root exudates); and (4) multivariate analysis supported the data provided that the proposed toolbox can be established and the field trial can be carried forward.
DOI: 10.1007/s11356-013-2154-3
PubMed: 24197963
Links to Exploration step
pubmed:24197963Le document en format XML
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uniqKey="Montereali M" first="Maria Rita" last="Montereali">Maria Rita Montereali</name></author><author><name sortKey="Marconi, Paola" sort="Marconi, Paola" uniqKey="Marconi P" first="Paola" last="Marconi">Paola Marconi</name></author><author><name sortKey="Tasso, Flavia" sort="Tasso, Flavia" uniqKey="Tasso F" first="Flavia" last="Tasso">Flavia Tasso</name></author><author><name sortKey="Turnau, Katarzyna" sort="Turnau, Katarzyna" uniqKey="Turnau K" first="Katarzyna" last="Turnau">Katarzyna Turnau</name></author><author><name sortKey="De Giudici, Giovanni" sort="De Giudici, Giovanni" uniqKey="De Giudici G" first="Giovanni" last="De Giudici">Giovanni De Giudici</name></author><author><name sortKey="Goralska, Katarzyna" sort="Goralska, Katarzyna" uniqKey="Goralska K" first="Katarzyna" last="Goralska">Katarzyna Goralska</name></author><author><name sortKey="Bevilacqua, Marta" sort="Bevilacqua, Marta" uniqKey="Bevilacqua M" first="Marta" last="Bevilacqua">Marta 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uniqKey="Sprocati A" first="Anna Rosa" last="Sprocati">Anna Rosa Sprocati</name><affiliation><nlm:affiliation>Environmental Characterization, Prevention and Recovery Unit, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Via Anguillarese, 301-00123, Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Alisi, Chiara" sort="Alisi, Chiara" uniqKey="Alisi C" first="Chiara" last="Alisi">Chiara Alisi</name></author><author><name sortKey="Pinto, Valentina" sort="Pinto, Valentina" uniqKey="Pinto V" first="Valentina" last="Pinto">Valentina Pinto</name></author><author><name sortKey="Montereali, Maria Rita" sort="Montereali, Maria Rita" uniqKey="Montereali M" first="Maria Rita" last="Montereali">Maria Rita Montereali</name></author><author><name sortKey="Marconi, Paola" sort="Marconi, Paola" uniqKey="Marconi P" first="Paola" last="Marconi">Paola Marconi</name></author><author><name sortKey="Tasso, Flavia" sort="Tasso, Flavia" uniqKey="Tasso F" first="Flavia" last="Tasso">Flavia Tasso</name></author><author><name sortKey="Turnau, Katarzyna" sort="Turnau, Katarzyna" uniqKey="Turnau K" first="Katarzyna" last="Turnau">Katarzyna Turnau</name></author><author><name sortKey="De Giudici, Giovanni" sort="De Giudici, Giovanni" uniqKey="De Giudici G" first="Giovanni" last="De Giudici">Giovanni De Giudici</name></author><author><name sortKey="Goralska, Katarzyna" sort="Goralska, Katarzyna" uniqKey="Goralska K" first="Katarzyna" last="Goralska">Katarzyna Goralska</name></author><author><name sortKey="Bevilacqua, Marta" sort="Bevilacqua, Marta" uniqKey="Bevilacqua M" first="Marta" last="Bevilacqua">Marta Bevilacqua</name></author><author><name sortKey="Marini, Federico" sort="Marini, Federico" uniqKey="Marini F" first="Federico" last="Marini">Federico Marini</name></author><author><name sortKey="Cremisini, Carlo" sort="Cremisini, Carlo" uniqKey="Cremisini C" first="Carlo" last="Cremisini">Carlo Cremisini</name></author></analytic><series><title level="j">Environmental science and pollution research international</title><idno type="eISSN">1614-7499</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteria (metabolism)</term><term>Biodegradation, Environmental (MeSH)</term><term>Euphorbia (growth & development)</term><term>Euphorbia (metabolism)</term><term>Euphorbia (microbiology)</term><term>Fungi (metabolism)</term><term>Italy (MeSH)</term><term>Metals, Heavy (analysis)</term><term>Mining (MeSH)</term><term>Multivariate Analysis (MeSH)</term><term>Mycorrhizae (metabolism)</term><term>Plant Roots (metabolism)</term><term>Soil (chemistry)</term><term>Soil Microbiology (MeSH)</term><term>Soil Pollutants (analysis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Metals, Heavy</term><term>Soil Pollutants</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Soil</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Italy</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Euphorbia</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacteria</term><term>Euphorbia</term><term>Fungi</term><term>Mycorrhizae</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Euphorbia</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Mining</term><term>Multivariate Analysis</term><term>Soil Microbiology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The paper describes the fieldwork at the Italian test site of the abandoned mine of sphalerite and galena in Ingurtosu (Sardinia), with the aim to assess the applicability of a "toolbox" to establish the optimized techniques for remediation of soils contaminated by mining activities. A preliminary characterization-including (hydro)geochemistry, heavy metal concentration and their mobility in soil, bioprospecting for microbiology and botany-provided a data set for the development of a toolbox to deliver a microbially assisted phytoremediation process. Euphorbia pithyusa was selected as an endemic pioneer plant to be associated with a bacterial consortium, established with ten selected native strains, including metal-tolerant bacteria and producers of plant growth factors. The toolbox was firstly assessed in a greenhouse pot experiment. A positive effect of bacterial inoculum on E. pithyusa germination and total plant survival was observed. E. pithyusa showed to be a well-performing metallophyte species, and only inoculated soil retained a microbial activity with a high functional diversity, expanding metabolic affinity also towards root exudates. These results supported the decision to proceed with a field trial, investigating different treatments used singly or in combination: bioaugmentation with bacterial consortia, mycorrhizal fungi and a commercial mineral amendment. Microbial activity in soil, plant physiological parameters and heavy metal content in plants and in soil were monitored. Five months after the beginning, an early assessment of the toolbox under field conditions was carried out. Despite the cold season (October-March), results suggested the following: (1) the field setup as well as the experimental design proved to be effective; (2) plant survival was satisfactory; (3) soil quality was increased and bioaugmentation improved microbial activity, expanding the metabolic competences towards plant interaction (root exudates); and (4) multivariate analysis supported the data provided that the proposed toolbox can be established and the field trial can be carried forward. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">24197963</PMID><DateCompleted><Year>2015</Year><Month>05</Month><Day>07</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1614-7499</ISSN><JournalIssue CitedMedium="Internet"><Volume>21</Volume><Issue>11</Issue><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>Environmental science and pollution research international</Title><ISOAbbreviation>Environ Sci Pollut Res Int</ISOAbbreviation></Journal><ArticleTitle>Assessment of the applicability of a "toolbox" designed for microbially assisted phytoremediation: the case study at Ingurtosu mining site (Italy).</ArticleTitle><Pagination><MedlinePgn>6939-51</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11356-013-2154-3</ELocationID><Abstract><AbstractText>The paper describes the fieldwork at the Italian test site of the abandoned mine of sphalerite and galena in Ingurtosu (Sardinia), with the aim to assess the applicability of a "toolbox" to establish the optimized techniques for remediation of soils contaminated by mining activities. A preliminary characterization-including (hydro)geochemistry, heavy metal concentration and their mobility in soil, bioprospecting for microbiology and botany-provided a data set for the development of a toolbox to deliver a microbially assisted phytoremediation process. Euphorbia pithyusa was selected as an endemic pioneer plant to be associated with a bacterial consortium, established with ten selected native strains, including metal-tolerant bacteria and producers of plant growth factors. The toolbox was firstly assessed in a greenhouse pot experiment. A positive effect of bacterial inoculum on E. pithyusa germination and total plant survival was observed. E. pithyusa showed to be a well-performing metallophyte species, and only inoculated soil retained a microbial activity with a high functional diversity, expanding metabolic affinity also towards root exudates. These results supported the decision to proceed with a field trial, investigating different treatments used singly or in combination: bioaugmentation with bacterial consortia, mycorrhizal fungi and a commercial mineral amendment. Microbial activity in soil, plant physiological parameters and heavy metal content in plants and in soil were monitored. Five months after the beginning, an early assessment of the toolbox under field conditions was carried out. Despite the cold season (October-March), results suggested the following: (1) the field setup as well as the experimental design proved to be effective; (2) plant survival was satisfactory; (3) soil quality was increased and bioaugmentation improved microbial activity, expanding the metabolic competences towards plant interaction (root exudates); and (4) multivariate analysis supported the data provided that the proposed toolbox can be established and the field trial can be carried forward. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sprocati</LastName><ForeName>Anna Rosa</ForeName><Initials>AR</Initials><AffiliationInfo><Affiliation>Environmental Characterization, Prevention and Recovery Unit, Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Via Anguillarese, 301-00123, Rome, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alisi</LastName><ForeName>Chiara</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Pinto</LastName><ForeName>Valentina</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Montereali</LastName><ForeName>Maria Rita</ForeName><Initials>MR</Initials></Author><Author ValidYN="Y"><LastName>Marconi</LastName><ForeName>Paola</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Tasso</LastName><ForeName>Flavia</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Turnau</LastName><ForeName>Katarzyna</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>De Giudici</LastName><ForeName>Giovanni</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Goralska</LastName><ForeName>Katarzyna</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Bevilacqua</LastName><ForeName>Marta</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Marini</LastName><ForeName>Federico</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Cremisini</LastName><ForeName>Carlo</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>10</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Environ Sci Pollut Res Int</MedlineTA><NlmUniqueID>9441769</NlmUniqueID><ISSNLinking>0944-1344</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012987">Soil</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012989">Soil Pollutants</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="Y">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D028481" MajorTopicYN="N">Euphorbia</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007558" MajorTopicYN="N" Type="Geographic">Italy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019216" MajorTopicYN="N">Metals, Heavy</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008906" MajorTopicYN="N">Mining</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015999" MajorTopicYN="N">Multivariate Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012987" MajorTopicYN="N">Soil</DescriptorName><QualifierName UI="Q000737" 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