Molecular cloning and functional analysis of a H(+)-dependent phosphate transporter gene from the ectomycorrhizal fungus Boletus edulis in southwest China.
Identifieur interne : 001856 ( Main/Corpus ); précédent : 001855; suivant : 001857Molecular cloning and functional analysis of a H(+)-dependent phosphate transporter gene from the ectomycorrhizal fungus Boletus edulis in southwest China.
Auteurs : Junling Wang ; Tao Li ; Xiaogang Wu ; Zhiwei ZhaoSource :
- Fungal biology [ 1878-6146 ]
English descriptors
- KwdEn :
- Basidiomycota (classification), Basidiomycota (genetics), Basidiomycota (metabolism), China (MeSH), Cloning, Molecular (MeSH), Fungal Proteins (genetics), Fungal Proteins (metabolism), Molecular Sequence Data (MeSH), Mycorrhizae (classification), Mycorrhizae (genetics), Mycorrhizae (metabolism), Phosphate Transport Proteins (genetics), Phosphate Transport Proteins (metabolism), Phosphates (metabolism), Phylogeny (MeSH).
- MESH :
- chemical , genetics : Fungal Proteins, Phosphate Transport Proteins.
- chemical , metabolism : Fungal Proteins, Phosphate Transport Proteins, Phosphates.
- geographic : China.
- classification : Basidiomycota, Mycorrhizae.
- genetics : Basidiomycota, Mycorrhizae.
- metabolism : Basidiomycota, Mycorrhizae.
- Cloning, Molecular, Molecular Sequence Data, Phylogeny.
Abstract
Phosphate transporters (PTs), as entry points for phosphorus (P) in organisms, are involved in a number of P nutrition processes such as phosphate uptake, transport, and transfer. In the study, a PT gene 1632 bp long (named BePT) was cloned, identified, and functionally characterized from Boletus edulis. BePT was expected to encode a polypeptide with 543 amino acid residues. The BePT polypeptide belonged to the major facilitator superfamily and showed a high degree of sequence identity to the Pht1 family. A topology model revealed that BePT exhibited 12 transmembrane helices, divided into two halves, and connected by a large hydrophilic loop in the middle. A yeast mutant complementation analysis suggested that BePT was a functional PT which mediated orthophosphate uptake of yeast at micromolar concentrations. Green fluorescent protein-BePT fusion proteins expressed were extensively restricted to the plasma membrane in BePT transformed yeast, and its activity was dependent on electrochemical membrane potential. In vitro, quantitative PCR confirmed that the expression of BePT was significantly upregulated at lower phosphorus availability, which may enhance phosphate uptake and transport under phosphate starvation. Our results suggest that BePT plays a key role in phosphate acquisition in the ectomycorrhizal fungus B. edulis.
DOI: 10.1016/j.funbio.2014.03.003
PubMed: 24863474
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular cloning and functional analysis of a H(+)-dependent phosphate transporter gene from the ectomycorrhizal fungus Boletus edulis in southwest China.</title><author><name sortKey="Wang, Junling" sort="Wang, Junling" uniqKey="Wang J" first="Junling" last="Wang">Junling Wang</name><affiliation><nlm:affiliation>Laboratory of Conservation and Utilization for Bioresources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan Province, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Tao" sort="Li, Tao" uniqKey="Li T" first="Tao" last="Li">Tao Li</name><affiliation><nlm:affiliation>Laboratory of Conservation and Utilization for Bioresources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan Province, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Xiaogang" sort="Wu, Xiaogang" uniqKey="Wu X" first="Xiaogang" last="Wu">Xiaogang Wu</name><affiliation><nlm:affiliation>Laboratory of Conservation and Utilization for Bioresources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan Province, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhao, Zhiwei" sort="Zhao, Zhiwei" uniqKey="Zhao Z" first="Zhiwei" last="Zhao">Zhiwei Zhao</name><affiliation><nlm:affiliation>Laboratory of Conservation and Utilization for Bioresources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan Province, PR China. Electronic address: zwzhao2001@yahoo.com.cn.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014 May-Jun</date><idno type="RBID">pubmed:24863474</idno><idno type="pmid">24863474</idno><idno type="doi">10.1016/j.funbio.2014.03.003</idno><idno type="wicri:Area/Main/Corpus">001856</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001856</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular cloning and functional analysis of a H(+)-dependent phosphate transporter gene from the ectomycorrhizal fungus Boletus edulis in southwest China.</title><author><name sortKey="Wang, Junling" sort="Wang, Junling" uniqKey="Wang J" first="Junling" last="Wang">Junling Wang</name><affiliation><nlm:affiliation>Laboratory of Conservation and Utilization for Bioresources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan Province, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Tao" sort="Li, Tao" uniqKey="Li T" first="Tao" last="Li">Tao Li</name><affiliation><nlm:affiliation>Laboratory of Conservation and Utilization for Bioresources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan Province, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Xiaogang" sort="Wu, Xiaogang" uniqKey="Wu X" first="Xiaogang" last="Wu">Xiaogang Wu</name><affiliation><nlm:affiliation>Laboratory of Conservation and Utilization for Bioresources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan Province, PR China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhao, Zhiwei" sort="Zhao, Zhiwei" uniqKey="Zhao Z" first="Zhiwei" last="Zhao">Zhiwei Zhao</name><affiliation><nlm:affiliation>Laboratory of Conservation and Utilization for Bioresources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan Province, PR China. Electronic address: zwzhao2001@yahoo.com.cn.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Fungal biology</title><idno type="ISSN">1878-6146</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (classification)</term><term>Basidiomycota (genetics)</term><term>Basidiomycota (metabolism)</term><term>China (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycorrhizae (classification)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (metabolism)</term><term>Phosphate Transport Proteins (genetics)</term><term>Phosphate Transport Proteins (metabolism)</term><term>Phosphates (metabolism)</term><term>Phylogeny (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>Phosphate Transport Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term><term>Phosphate Transport Proteins</term><term>Phosphates</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Basidiomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Molecular Sequence Data</term><term>Phylogeny</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phosphate transporters (PTs), as entry points for phosphorus (P) in organisms, are involved in a number of P nutrition processes such as phosphate uptake, transport, and transfer. In the study, a PT gene 1632 bp long (named BePT) was cloned, identified, and functionally characterized from Boletus edulis. BePT was expected to encode a polypeptide with 543 amino acid residues. The BePT polypeptide belonged to the major facilitator superfamily and showed a high degree of sequence identity to the Pht1 family. A topology model revealed that BePT exhibited 12 transmembrane helices, divided into two halves, and connected by a large hydrophilic loop in the middle. A yeast mutant complementation analysis suggested that BePT was a functional PT which mediated orthophosphate uptake of yeast at micromolar concentrations. Green fluorescent protein-BePT fusion proteins expressed were extensively restricted to the plasma membrane in BePT transformed yeast, and its activity was dependent on electrochemical membrane potential. In vitro, quantitative PCR confirmed that the expression of BePT was significantly upregulated at lower phosphorus availability, which may enhance phosphate uptake and transport under phosphate starvation. Our results suggest that BePT plays a key role in phosphate acquisition in the ectomycorrhizal fungus B. edulis. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24863474</PMID><DateCompleted><Year>2015</Year><Month>01</Month><Day>02</Day></DateCompleted><DateRevised><Year>2014</Year><Month>05</Month><Day>27</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1878-6146</ISSN><JournalIssue CitedMedium="Internet"><Volume>118</Volume><Issue>5-6</Issue><PubDate><MedlineDate>2014 May-Jun</MedlineDate></PubDate></JournalIssue><Title>Fungal biology</Title><ISOAbbreviation>Fungal Biol</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning and functional analysis of a H(+)-dependent phosphate transporter gene from the ectomycorrhizal fungus Boletus edulis in southwest China.</ArticleTitle><Pagination><MedlinePgn>453-61</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.funbio.2014.03.003</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1878-6146(14)00044-0</ELocationID><Abstract><AbstractText>Phosphate transporters (PTs), as entry points for phosphorus (P) in organisms, are involved in a number of P nutrition processes such as phosphate uptake, transport, and transfer. In the study, a PT gene 1632 bp long (named BePT) was cloned, identified, and functionally characterized from Boletus edulis. BePT was expected to encode a polypeptide with 543 amino acid residues. The BePT polypeptide belonged to the major facilitator superfamily and showed a high degree of sequence identity to the Pht1 family. A topology model revealed that BePT exhibited 12 transmembrane helices, divided into two halves, and connected by a large hydrophilic loop in the middle. A yeast mutant complementation analysis suggested that BePT was a functional PT which mediated orthophosphate uptake of yeast at micromolar concentrations. Green fluorescent protein-BePT fusion proteins expressed were extensively restricted to the plasma membrane in BePT transformed yeast, and its activity was dependent on electrochemical membrane potential. In vitro, quantitative PCR confirmed that the expression of BePT was significantly upregulated at lower phosphorus availability, which may enhance phosphate uptake and transport under phosphate starvation. Our results suggest that BePT plays a key role in phosphate acquisition in the ectomycorrhizal fungus B. edulis. </AbstractText><CopyrightInformation>Copyright © 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Junling</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Conservation and Utilization for Bioresources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan Province, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Tao</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Laboratory of Conservation and Utilization for Bioresources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan Province, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Xiaogang</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Laboratory of Conservation and Utilization for Bioresources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan Province, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Zhiwei</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Laboratory of Conservation and Utilization for Bioresources, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, Yunnan Province, PR China. 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