Polyphosphate accumulation is driven by transcriptome alterations that lead to near-synchronous and near-equivalent uptake of inorganic cations in an arbuscular mycorrhizal fungus.
Identifieur interne : 001789 ( Main/Corpus ); précédent : 001788; suivant : 001790Polyphosphate accumulation is driven by transcriptome alterations that lead to near-synchronous and near-equivalent uptake of inorganic cations in an arbuscular mycorrhizal fungus.
Auteurs : Yusuke Kikuchi ; Nowaki Hijikata ; Kaede Yokoyama ; Ryo Ohtomo ; Yoshihiro Handa ; Masayoshi Kawaguchi ; Katsuharu Saito ; Tatsuhiro EzawaSource :
- The New phytologist [ 1469-8137 ] ; 2014.
English descriptors
- KwdEn :
- Amino Acids (metabolism), Biological Transport (MeSH), Cations (metabolism), Fungi (metabolism), Gene Expression Regulation, Fungal (physiology), Lotus (microbiology), Mycorrhizae (metabolism), Nitrogen (metabolism), Polyphosphates (metabolism), RNA, Fungal (genetics), RNA, Fungal (metabolism), Transcriptome (MeSH).
- MESH :
- chemical , genetics : RNA, Fungal.
- chemical , metabolism : Amino Acids, Cations, Nitrogen, Polyphosphates, RNA, Fungal.
- metabolism : Fungi, Mycorrhizae.
- microbiology : Lotus.
- physiology : Gene Expression Regulation, Fungal.
- Biological Transport, Transcriptome.
Abstract
Arbuscular mycorrhizal (AM) fungi accumulate a massive amount of phosphate as polyphosphate to deliver to the host, but the underlying physiological and molecular mechanisms have yet to be elucidated. In the present study, the dynamics of cationic components during polyphosphate accumulation were investigated in conjunction with transcriptome analysis. Rhizophagus sp. HR1 was grown with Lotus japonicus under phosphorus-deficient conditions, and extraradical mycelia were harvested after phosphate application at prescribed intervals. Levels of polyphosphate, inorganic cations and amino acids were measured, and RNA-Seq was performed on the Illumina platform. Phosphate application triggered not only polyphosphate accumulation but also near-synchronous and near-equivalent uptake of Na(+) , K(+) , Ca(2+) and Mg(2+) , whereas no distinct changes in the levels of amino acids were observed. During polyphosphate accumulation, the genes responsible for mineral uptake, phosphate and nitrogen metabolism and the maintenance of cellular homeostasis were up-regulated. The results suggest that inorganic cations play a major role in neutralizing the negative charge of polyphosphate, and these processes are achieved by the orchestrated regulation of gene expression. Our findings provide, for the first time, a global picture of the cellular response to increased phosphate availability, which is the initial process of nutrient delivery in the associations.
DOI: 10.1111/nph.12937
PubMed: 25039900
Links to Exploration step
pubmed:25039900Le document en format XML
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sort="Handa, Yoshihiro" uniqKey="Handa Y" first="Yoshihiro" last="Handa">Yoshihiro Handa</name></author><author><name sortKey="Kawaguchi, Masayoshi" sort="Kawaguchi, Masayoshi" uniqKey="Kawaguchi M" first="Masayoshi" last="Kawaguchi">Masayoshi Kawaguchi</name></author><author><name sortKey="Saito, Katsuharu" sort="Saito, Katsuharu" uniqKey="Saito K" first="Katsuharu" last="Saito">Katsuharu Saito</name></author><author><name sortKey="Ezawa, Tatsuhiro" sort="Ezawa, Tatsuhiro" uniqKey="Ezawa T" first="Tatsuhiro" last="Ezawa">Tatsuhiro Ezawa</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25039900</idno><idno type="pmid">25039900</idno><idno type="doi">10.1111/nph.12937</idno><idno type="wicri:Area/Main/Corpus">001789</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001789</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Polyphosphate accumulation is driven by transcriptome alterations that lead to near-synchronous and near-equivalent uptake of inorganic cations in an arbuscular mycorrhizal fungus.</title><author><name sortKey="Kikuchi, Yusuke" sort="Kikuchi, Yusuke" uniqKey="Kikuchi Y" first="Yusuke" last="Kikuchi">Yusuke Kikuchi</name><affiliation><nlm:affiliation>Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Hijikata, Nowaki" sort="Hijikata, Nowaki" uniqKey="Hijikata N" first="Nowaki" last="Hijikata">Nowaki Hijikata</name></author><author><name sortKey="Yokoyama, Kaede" sort="Yokoyama, Kaede" uniqKey="Yokoyama K" first="Kaede" last="Yokoyama">Kaede Yokoyama</name></author><author><name sortKey="Ohtomo, Ryo" sort="Ohtomo, Ryo" uniqKey="Ohtomo R" first="Ryo" last="Ohtomo">Ryo Ohtomo</name></author><author><name sortKey="Handa, Yoshihiro" sort="Handa, Yoshihiro" uniqKey="Handa Y" first="Yoshihiro" last="Handa">Yoshihiro Handa</name></author><author><name sortKey="Kawaguchi, Masayoshi" sort="Kawaguchi, Masayoshi" uniqKey="Kawaguchi M" first="Masayoshi" last="Kawaguchi">Masayoshi Kawaguchi</name></author><author><name sortKey="Saito, Katsuharu" sort="Saito, Katsuharu" uniqKey="Saito K" first="Katsuharu" last="Saito">Katsuharu Saito</name></author><author><name sortKey="Ezawa, Tatsuhiro" sort="Ezawa, Tatsuhiro" uniqKey="Ezawa T" first="Tatsuhiro" last="Ezawa">Tatsuhiro Ezawa</name></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids (metabolism)</term><term>Biological Transport (MeSH)</term><term>Cations (metabolism)</term><term>Fungi (metabolism)</term><term>Gene Expression Regulation, Fungal (physiology)</term><term>Lotus (microbiology)</term><term>Mycorrhizae (metabolism)</term><term>Nitrogen (metabolism)</term><term>Polyphosphates (metabolism)</term><term>RNA, Fungal (genetics)</term><term>RNA, Fungal (metabolism)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Fungal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amino Acids</term><term>Cations</term><term>Nitrogen</term><term>Polyphosphates</term><term>RNA, Fungal</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fungi</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lotus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Fungal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Transport</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Arbuscular mycorrhizal (AM) fungi accumulate a massive amount of phosphate as polyphosphate to deliver to the host, but the underlying physiological and molecular mechanisms have yet to be elucidated. In the present study, the dynamics of cationic components during polyphosphate accumulation were investigated in conjunction with transcriptome analysis. Rhizophagus sp. HR1 was grown with Lotus japonicus under phosphorus-deficient conditions, and extraradical mycelia were harvested after phosphate application at prescribed intervals. Levels of polyphosphate, inorganic cations and amino acids were measured, and RNA-Seq was performed on the Illumina platform. Phosphate application triggered not only polyphosphate accumulation but also near-synchronous and near-equivalent uptake of Na(+) , K(+) , Ca(2+) and Mg(2+) , whereas no distinct changes in the levels of amino acids were observed. During polyphosphate accumulation, the genes responsible for mineral uptake, phosphate and nitrogen metabolism and the maintenance of cellular homeostasis were up-regulated. The results suggest that inorganic cations play a major role in neutralizing the negative charge of polyphosphate, and these processes are achieved by the orchestrated regulation of gene expression. Our findings provide, for the first time, a global picture of the cellular response to increased phosphate availability, which is the initial process of nutrient delivery in the associations.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25039900</PMID><DateCompleted><Year>2015</Year><Month>06</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>204</Volume><Issue>3</Issue><PubDate><Year>2014</Year><Month>Nov</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Polyphosphate accumulation is driven by transcriptome alterations that lead to near-synchronous and near-equivalent uptake of inorganic cations in an arbuscular mycorrhizal fungus.</ArticleTitle><Pagination><MedlinePgn>638-49</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/nph.12937</ELocationID><Abstract><AbstractText>Arbuscular mycorrhizal (AM) fungi accumulate a massive amount of phosphate as polyphosphate to deliver to the host, but the underlying physiological and molecular mechanisms have yet to be elucidated. In the present study, the dynamics of cationic components during polyphosphate accumulation were investigated in conjunction with transcriptome analysis. Rhizophagus sp. HR1 was grown with Lotus japonicus under phosphorus-deficient conditions, and extraradical mycelia were harvested after phosphate application at prescribed intervals. Levels of polyphosphate, inorganic cations and amino acids were measured, and RNA-Seq was performed on the Illumina platform. Phosphate application triggered not only polyphosphate accumulation but also near-synchronous and near-equivalent uptake of Na(+) , K(+) , Ca(2+) and Mg(2+) , whereas no distinct changes in the levels of amino acids were observed. During polyphosphate accumulation, the genes responsible for mineral uptake, phosphate and nitrogen metabolism and the maintenance of cellular homeostasis were up-regulated. The results suggest that inorganic cations play a major role in neutralizing the negative charge of polyphosphate, and these processes are achieved by the orchestrated regulation of gene expression. Our findings provide, for the first time, a global picture of the cellular response to increased phosphate availability, which is the initial process of nutrient delivery in the associations.</AbstractText><CopyrightInformation>© 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kikuchi</LastName><ForeName>Yusuke</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hijikata</LastName><ForeName>Nowaki</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Yokoyama</LastName><ForeName>Kaede</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Ohtomo</LastName><ForeName>Ryo</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Handa</LastName><ForeName>Yoshihiro</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Kawaguchi</LastName><ForeName>Masayoshi</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Saito</LastName><ForeName>Katsuharu</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Ezawa</LastName><ForeName>Tatsuhiro</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>07</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000596">Amino Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002412">Cations</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000070116" MajorTopicYN="N">Lotus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011122" MajorTopicYN="N">Polyphosphates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012331" 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